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International Journal of Applied Biology
Published by Universitas Hasanuddin
ISSN : 25802410     EISSN : 25802119     DOI : -
Core Subject : Science, Agriculture, Social, Engineering,
Agriculture, Biological Sciences & Forestry Automotive Engineering Energy Environmental Science
International Journal of Applied Biology is an international, peer reviewed journal, publishing high-quality research. International Journal of Applied Biology only publishes manuscripts in English. Please see the journal's Focus & Scope for information about its Scope and peer-review policy. International Journal of Applied Biology is available in print and online version.
Arjuna Subject : -
Articles 168 Documents
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The Potential of Algae Spyrogyra peipingensis to Produce Nanogold in Batch Culture Medium Arbaina Syahdinnur; Agusrahman Ekaputra Abas; Chitriani Armidha
International Journal of Applied Biology Vol. 2 No. 1 (2018): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v2i1.4450

Abstract

The biosynthesis of gold nanoparticles by using algae Spirogyra peipingensis was conducted. This research aimed to determine biosynthesis of gold nanoparticles by using algae Spirogyra peipingensis with different concentration and incubation time. The synthesis of gold nanoparticles using HauCl4 solution with variations of concentration 5 ppm, 10 ppm, 15 ppm and 20 ppm respectively in 1 liter of aquabidest. Algae Spirogyra peipingensis was grown in HauCl4 medium with the addition of 0.2 gr Sulfahri-01 nutrient. Then each 5 gram Spirogyra algae incubated in HAuCl4 medium with the addition of Sulfahri-01 0.2 gram nutrients. 4 hours long incubation under exposure to sunlight. Nanoparticle size determination is done by looking at the color that appears in the solution. From the results of the research, it is known that Spirogyra peipingensis algae is able to synthesize gold nanoparticles characterized by the color change in algae biomass from green to purple color after being treated and forming gold nanoparticles with size 40-60 NM. The best-used HAuCl4 consent is 5 ppm with the smallest particle size. KeywordIntracelullar, Spirogyra peipingensis,HAuCl4,Biosynthesis.  Article HistoryReceived 8 May 2017 Accepted 3 July 2017                                                                               
The Analysis of β-cryptoxanthin and Zeaxanthin using HPLC in the Accumulation of Orange Color on Lowland Citrus Inanpi Hidayati Sumiasih; Roedhy Poerwanto; Darda Efendi; Andria Agusta; Sri Yuliani
International Journal of Applied Biology Vol. 1 No. 2 (2017): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v1i2.3066

Abstract

Citrus peel color is one of the main quality attributes which was caused by the accumulation of carotenoids and its derivatives, especially β-citraurine. It makes citrus peel color looks attractive (orange). The orange color is a mixture of β-cryptoxanthin with β-citraurin. The objectives of this study were (1) to observe the effect of precooling and duration of proper ethylene exposure in the formation of orange color on citrus peel, (2) to identify and determine the β-cryptoxanthin content and total chlorophyll on citrus peel. Citrus was from Tuban, East Java while the study was conducted at PKHT IPB and LIPI. Precooling and without precooling treatment prior to injection of 100 ppm of ethylene exposed at 15 °C, duration of exposure control (0), 24, and 48 hours. The results show that the best color of the Citrus Color Index (CCI) is the precooling treatment and the duration of ethylene exposure for 24 hours, which can reduce total chlorophyll content about 8 times and proved to increase β-cryptoxanthin pigment content five times in accelerating the formation of orange citrus reticulata peel color to bright orange. Degreening has no significant effect on total dissolved solids and the firmness level of citrus fruits.Keyword: β-cryptoxhantin; citrus; chlorophyll; degreening; ethylene zeaxanthin.
Biodiversity and distribution of gastropods at seagrass meadow of Balangdatu waters Tanakeke Island South Sulawesi Indonesia Magdalena Litaay; Marwa Deviana; Dody Priosambodo
International Journal of Applied Biology Vol. 1 No. 2 (2017): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v1i2.3134

Abstract

The research about the biodiversity of gastropod has been conducted in seagrass meadow of Balangdatu waters, Tanakeke Island, South Sulawesi. The research aims to assess the diversity of gastropod species in Balangdatu waters. Sampling was conducted using quadrate transect method systematically. Three replicates of transect were applied for each station. The result indicates there were 34 species of gastropods from 14 genera and 14 families were found. Diversity index from every station varies from 1,661 to 2, 899. These values range from low to moderate. The diversity, Evenness, and dominance indices showed that Balangdatu waters still in good condition and sustain habitat for gastropod.
The Effect of Method and Ripening Duration on the Content of β-Carotene of Fe’i Banana (Musa troglodytarum L.) Fredy Leiwakabessy
International Journal of Applied Biology Vol. 2 No. 1 (2018): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v2i1.4020

Abstract

Research was conducted to determine the effect of the method and duration of ripening on the content of β-carotene of Fe’i banana, as well as determine the method and duration of ripening is effective in maintaining the nutritional value of Fe’i Banana. Through laboratory experimental with randomized complete design consisting of 19 groups of treatments and three repetitions, the data obtained were analyzed using statistical tests ANOVA and continued with Duncan test. Results of the analysis showed that method ofripening statistically significant difference (p <0,05) on the content of β-carotene in the banana sticks sky. Long ripening length statistically significant difference (p <0, 05) on the content of β-carotene in the Fe’i Banana. Grilled ripening method is more effective against β-carotene content in the Fe’i Banana and takes1 0 minutes of ripening length more effectively on the content of β-carotene on a Fe’i Banana.
Application of Cigeratte Filter from Algae Spyrogira peipingensis to Reduce Nicotine, Tar, and Carbon Monoxide Syamsul Bahri
International Journal of Applied Biology Vol. 1 No. 1 (2017): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v1i1.2014

Abstract

Algae Spirogyra peipingensis is a group of green algae from the order Zygnematales. Spirogyra algae serves as a bioremediation agent. In addition Spirogyra algae has great potential in reducing toxin levels in cigarette smoke. Because the biomass of alga Spirogyra has an irregular shaped surface texture and consists of amino acid, amide, carboxylic, hydroxyl, and carbonyl group that can bind various heavy metals and other harmful compounds. Alga Spirogyra peipingensis samples used are algae from Bone, South Sulawesi. Prior to the trial, the first pretreatment and processed to become powder. Algae powder was then applied to cigarette filters and tested on several respondents. Results showed that there was a significant decrease  tar, nicotine and carbon monoxide levels in cigarette smoke by using Spirogyra peipingensis algae but the most significant decrease of carbon monoxide, while tar and nicotine decreased even though not as high as carbon monoxide. 
Development Michrosphere Bioadhesive Delivery System From Secondary Metabolit Actynomycetes M10a Rhizosphere Mangrove As Supportive Therapy of Colon Cancer Patients Fajrin Hasan Basri
International Journal of Applied Biology Vol. 2 No. 1 (2018): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v2i1.4615

Abstract

Colon cancer is a cancer that occurs in the large intestine when abnormal cells grow in the colon or rectum layer.  Inflammation Symptoms  in the colon are usually characterized by a polyp that has cancer risk. Approximately 96% of colon cancer cases are caused by adenocarcinomas that develop from the glandular tissue. Utilization of secondary metabolites from Actynomycetes Mangrove forests can be used as supportive therapy for colon cancer patients that have already through apoptotic testing so it can directly treat the target of illness. This  study was prepared in the form of  Michrosphere Bioadhesive  using secondary metabolite  Actynomycetes M10a rhizosphere mangrove. The purpose of this study was to obtain the development of  Michosphere Bioadhesive  preparations from secondary metabolites  Actynomycetes M10a  rhizosphere mangrove  as supportive therapy for colon cancer patients. Method of the research is base on Laboratory experiment which started with Purification  of  Actynomycetes  isolate, Secondary Metabolite Fermentation,  Michrosphere Bioadhesive  Formulation and evaluation of  Michrosphere  Bioadhesive  preparation. The concentration of secondary metabolite Actynomycetes M10a rhizosphere mangrove that been used for each formula are 400 μl, 500 μl, and 600 μl, where the percentage of submerged in F1, F2, and F3 are 93,94%, 96,96%, and 94, respectively, 12%. Particular Particle Size Distribution is F1 (3.1μm), F2 (4.4 μm), F3 (3.9 μm). The rest angles obtained are F1 (0.002O), F2 (0.002O), F3 (0.0016O), respectively. Applied efficiency was obtained by F1 (214 nm), F2 (210 nm), F3 (211 nm). Dissolution results show the absorbance results at 266 nm wavelength. From the results obtained that meet the specifications seen in Formula 2.  Keywords: Colon Cancer,  Actynomycetes  M10a  rhizosphere mangrove, Michosphere Bioadhesive.
Agronomic performance and genotypic diversity for morphological traits among early maize genotypes Bhim Nath Adhikari; Jiban Shrestha; Bishal Dhakal; Bishnu Prasad Joshi; Naba Raj Bhatta
International Journal of Applied Biology Vol. 2 No. 2 (2018): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v2i2.5633

Abstract

Detailed information on the genetic diversity between maize germplasm (Zea mays L.) is useful for their systematic and efficient use in breeding programs. Fourteen early maize genotypes were studied to assess their performance and genotypic diversity at Doti, Nepal in 2015. Days to tasseling, days to silking, plant height, ear height, ear length, ear diameter and grain yield were significant among genotypes. Genotype SO3TEY-PO-BM, COMPOL-NIOBP and ACROSS-99402 were found higher yielder with earlier maturity. Days to tasseling (0.85), days to silking (0.82), plant height (0.79), ear length (0.71) and ear diameter (0.66) were found highly heritable traits. Grain yield (0.39) and ear height (0.47) medium and remaining traits showed low heritability. High PCV was observed for grain yield (35.10%), number of plants/plot (34.46%), tesseling silking interval (26.85%), harvested ears/plot (24.45%) and husk cover rating (22.85%) where other traits showed medium to low PCV. Grain yield showed high GCV (21.96%), ear height and husk cover had medium and remaining traits showed low GCV (<10%). Plant height (r₌0.498), harvested plants/plot (r₌0.412), harvested ear/plot (r₌0.762), ear length (r₌0.472) and ear diameter (r₌0.470) showed significant positive correlation with grain yield. The yield can be improved if selection applied in favor of those yield components.
Vegetation structure, species diversity, and mangrove zonation patterns in the Tanjung Panjang Nature Reserve Area, Gorontalo, Indonesia Dewi Wahyuni Baderan; Ramli Utina; Nuraini Lapolo
International Journal of Applied Biology Vol. 2 No. 2 (2018): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v2i2.5752

Abstract

This study aimed to analyze the structure of vegetation, diversity and patterns of mangrove zonation in the Tanjung Panjang Nature Reserve area. Data collection of mangrove vegetation structure was carried out by using the line transect method (plot measuring 20 m x 20 m), measurement of environmental parameters that supported mangrove life, and 12 soil samples carried out by laboratory tests. The sample distribution was 18 research sample points. The results showed that mangrove vegetation density both at the level of trees, saplings and seedlings was in the category of total damage with density values at tree level 0.04-0.22 ind/ha), sapling 0- 0.07ind/ha), and seedlings 0.02-0.08 ind/Ha). The percentage of closure types ranged from 0% - 38%, meaning that the criteria of damage was rare, which was <50%, and some were included in the criteria of total damage, which was 0%. The index of mangrove species diversity was sequential starting from the level of trees, saplings and seedlings, which were 0.37-1.53, 0.00-0.31, and 0.00, where the tree level diversity index was in the medium category, and the sapling and seedling levels were in the low category.
Diversity of Ordo Lepidoptera In Mangkok Resort, Sebangau National Park, Central Kalimantan Achmad Faqih
International Journal of Applied Biology Vol. 2 No. 2 (2018): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v2i2.5815

Abstract

The diversity of the butterfly in Sebangau National Park, particularly in the Mangkok Resort area has different morphological features in each species. The differences distinguish each species between families. Steps to know the diversity species or populations can be measured and identified based on similarities or differences in Shannon-Wiener's diversity analysis. This research was conducted by random purposive sampling using insect net and food trap. Observation and sampling were conducted in the area of natural forest and forest restoration during the dry and rainy seasons. The results showed, the species obtained and identified as many as 25 species from 4 families namely Papilionidae, Nymphalidae, Pieridae and Lycaenidae. Butterflies obtained from food trap are 24 individuals from 5 species. The level of diversity, evenness and richness of the species of butterflies in each region during the rainy and dry seasons which are the location of the study are included in the "medium" category with the average value of the diversity index, the evenness and the richness of the butterfly species of 2.502. The relative dominance and relative abundance of the butterfly species in the Mangkok resort shows no species communities that are extreme dominant from species other.
Productivity of maize (Zea mays L.) as affected by varieties and sowing dates Dil Bahadur Gurung; Balram Bhandari; Jiban Shrestha; Mahendra Prasad Tripathi
International Journal of Applied Biology Vol. 2 No. 2 (2018): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v2i2.4673

Abstract

Genotypic yield potential of maize varieties is greatly affected by sowing dates. In order to investigate the effects of sowing dates and varieties on the grain yield of maize, the field experiment was carried out at research field of National Maize Research Program (NMRP), Rampur, Chitwan, Nepal from April 2009 to March 2010.  Three varieties namely Rampur Composite, Arun-2 and Gaurav were sown at every week. The results of experiment showed that interaction effect of variety and sowing date on grain yield of maize was significant. Rampur Composite produced highest grain yield (6.1 t/ha) in August and lowest yield (2.6 t/ha) in May. Similarly Arun-2 produced highest yield (4.6 t/ha) in August and lowest yield (2.1 t/ha) in May. Gaurav produced highest grain yield (5.1 t/ha) in September followed by 4.9, 4.8 and 4.6 t/ha in February, July and August respectively and lowest yield (1.5 t/ha) in November. The sowing date was highly significant on grain production. The highest grain production was 5.1 t/ha in August followed by in February (4.9 t/ha), September (4.6 t/ha) and March (4.4 t/ha) respectively. The lowest grain yield was produced in May (2.4 t/ha). Therefore it was concluded that August planting was best for higher grain production of maize varieties (Rampur Composite, Arun-2 and Gaurav) in terai region of Nepal.

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