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International Journal of Applied Biology
Published by Universitas Hasanuddin
ISSN : 25802410     EISSN : 25802119     DOI : -
International Journal of Applied Biology is an international, peer reviewed journal, publishing high-quality research. International Journal of Applied Biology only publishes manuscripts in English. Please see the journal's Focus & Scope for information about its Scope and peer-review policy. International Journal of Applied Biology is available in print and online version.
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Articles 168 Documents
Performance Assessment of Public Health Center Madising Na Mario Parepare, South Sulawesi, Indonesia Ajeng Dyah Pythaloka; Muhammad Yusri Abadi; Darmawansyah Darmawansyah
International Journal of Applied Biology Vol. 3 No. 1 (2019): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v3i1.5927

Abstract

Implement health efforts both public health efforts are first rate and first rate individual health efforts are needed health center management be integrated and continuous in order to produce the performance of Public Health Center effective and efficient. This study aims to find out about the picture of the level of achievement of performance results pertaining to quality health center health center services, management of health centers and health care Public Health Center Madising Na Mario Pare-pare. This research method is quantitative descriptive method, whereby it purports to describe the level of achievement of the performance results of health centers. From the research achievement of quality performance owned health center services obtained an average value of 9,7 performance with good performance categories. Public Health Center management obtained an average performance score of 10 with a good performance categories. Health services obtained the value of the average performance of 70,9% with less performance categories. Therefore, efforts to improve the performance of health centers needed professional human resources and improvement of health facilities, especially the budget required.
Analysis of Flavonoid Levels of Enhalus acoroides in Different Coastal Waters in Ambon Island, Indonesia Prelly MJ Tuapattinaya; Dominggus Rumahlatu
International Journal of Applied Biology Vol. 3 No. 1 (2019): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Seagrass contains bioactive compounds or secondary metabolites which can be used as potential drugs. This research aims at analyzing the levels of flavonoids of the leaves of seagrass Enhalus acoroides in three different coastal waters. The sampling of seagrass leaves was carried out in three locations in the coastal waters of Ambon Island, namely the coastal waters of Galala, Rutong, and Waai. The levels of flavonoid were identified using microscopic-microchemical methods. The test results of the flavonoid levels were analyzed descriptively. The results of this research showed that samples of seagrass leaves from the coastal waters of Galala, Rutong, and Waai, after added with NaOH, AlCl3, and NH3reagents showed a color change. It means that the samples from the three coastal waters were positive to contain flavonoids. Therefore, the analysis was continued to determine the average levels of flavonoids of E. acoroides. The average levels of flavonoid in the three coastal waters of Galala, Rutong, and Waai were 0.0192%, 0.1475%, and 3.5697% respectively. The environmental conditions and substrate of seagrass E. acoroides in the coastal waters of Rutong and Waai caused higher levels of flavonoids than the levels of flavonoids in the coastal waters of Galala.
Antioxidant Activity In Combination Extract of Acorus Calamus L. (Dlingu) and Allium Sativum (Garlic) Pramita Laksitarahmi Isrianto; Putu Oky Ari Tania
International Journal of Applied Biology Vol. 3 No. 1 (2019): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v3i1.5471

Abstract

One of the efforts to optimize the utilization of natural materials that is used them as medicinal plants, including Dlingu (Acorus calamus L.) and Garlic (Allium sativum) which have the potential as antioxidants. This study aims to determine the effectiveness of a combination extract of Acorus calamus L. (Dlingu) and Allium sativum (Garlic) on antioxidant activity. This research is an experimental research which is the test of antioxidant content and antioxidant activity test with 5 series of concentrations and 4 times of repetition. The research materials, namely dlingu stem and single clove garlic tubers were extracted, phytochemical screening was carried out and then antiradical free IC50 was determined. Based on the test results of the chemical extract of dligu and single clove garlic, the highest content was alkaloid 5.16%, tannin 4.05%, saponin 3.01%, and flavonoid 2.18%. The test results of the chemical antioxidant activity of extracts dligu and single clove garlic contain IC50 values of 17.062 ppm, including a very strong class of antioxidants.Keywords: antioxidant, Dlingu, Single Clove (Male) Garlic.
The Ability of Ferns to Accumulate Heavy Metals (Hg, Pb And Cd) In The Waters of The Gorontalo River muhamad iksan; La Aba Aba; Kusrini Kusrini
International Journal of Applied Biology Vol. 3 No. 1 (2019): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v3i1.5930

Abstract

Sustainable  development  can be caused by more human’s activity. This activity, it will have an impact on the surrounding  environment  which  can  disrupt  the  stability  of  the  ecosystem,  one  of  which  is  the  river  water ecosystem.  The  research  aims  to  determine  the  ability  of  ferns  in  accumulating  heavy  metals  so  that  they  can  be  used  as  indicators  of  the  depletion  of  river  waters  ecosystem  environments  and  can  be  used  as  fitoremediant areas  of  waters  which  accumulate  heavy  metals.  This  research   is  descriptive  quantitative.  Retrieval  of  initial  data or  sample  in  this  study  used  survey  methods  and  random  sampling  techniques,  testing  samples by  using   the Ranger  X-ray  Flourenciece  (XRF)  method.  Based  on  the  results  of  the  study  that, ferns  can  accumulate  heavy metals,  the  parts  that  accumulate  are  located  below  the  root  surface  and  above  the  soil  surface,  namely stems and  leaves.  In  the  roots  have  more  amount  in  accumulating  heavy  metals  than  in  the  stem  and  leaves, this  is because  the  metal  is  indicated  on  the  soil.  From  these  results,  it  can  be  concluded  that  ferns  can  be used as water  purification  plants  in  river  ecosystems  that  are  indicated  by  heavy  metals.
Biomass and Carbon Uptake of Mangrove Forests Pohorua Village, Muna Regency muhamad iksan
International Journal of Applied Biology Vol. 3 No. 2 (2019): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v3i2.8448

Abstract

Environmental conditions caused by air pollution are so large that it impacts on changes in the ecosystem that affects all aspects of human life. Climate change is caused by increasing greenhouse gases in the earth's atmosphere because the Earth's atmosphere receives more carbon than it releases. This study aims to determine the potential of biomass and carbon uptake in mangrove stems in Pohorua village, Muna Regency. The research is quantitative descriptive, sampling using the Point Center Quarted Method (PCQM) technique measured around the height of the chest height mangrove tree (DBH). Data analysis was performed using an allometric equation in which each mangrove plant has a specific gravity. Carbon uptake found in mangroves stored in the roots, stems and leaves of mangrove plants, the results of the study showed that mangroves can absorb carbon quickly.
Genetic inheritance of multiple traits of blast, bacteria leaf blight resistant and drought tolerant rice lines Mohd Rafii Yusop
International Journal of Applied Biology Vol. 3 No. 2 (2019): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v3i2.7760

Abstract

Rice (Oryza sativa L.) is a cereal and staple food crop of over half of the world’s population. Blast, bacteria leaf blight and drought stresses affect yield of rice drastically ranging from 1-100% loss depending on the severity of disease and water deficit condition. Resistance and tolerance high yielding varieties of blast (Putra1) and drought (MR219 IR99784-156-137-1-3) respectively and also IRBB60 (bacteria leaf blight) were used. The research considered the genetic inheritance of the new improved lines and their interactions. Pedigree breeding method was used to develop two single, double and three-way (and reciprocal) crosses through marker-assisted selection. Southern blot analysis was used to determine success of introgression of resistance/tolerance genes/QTLs and selection, also validated by phenotyped results. Agro-morphological and yield parameters of the various populations were analysed. The results indicated levels of significant differences amongst and between treatments for non-drought stress (NS) and reproductive drought stress (RS) and their interactions. There were significant variation among parents and improved lines on some traits in NS treatment, but RS significantly affected parameters of DF, FFG, YM and most especially the susceptible parent, while the improved lines were tolerant.  Significant interactions was recorded (P≤0.05) between treatment and variety (Trt*Var.) on PL, T, FFG and GLW. Cluster analysis and PCA of relationship among the 9 traits in the two treatments revealed that each of single, double and three-way (and reciprocal) crosses had good lines either under NS and RS. 
Study of The Effect of Sugar and Lime Juice Proportion on the Quality of Starf Ruit Sorbet Hermawan Seftiono; Gracecilia Yohanna Panjaitan; Inanpi Hidayati Sumiasih
International Journal of Applied Biology Vol. 4 No. 1 (2020): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v4i1.9181

Abstract

Star fruit is a non-seasonal fruit that can be harvested three to four times a year. One possible way to process a star fruit is to make it into sorbet. Additional ingredients such as sugar and lime juice will help improve the quality of star fruit sorbet. The purpose of this study was to acquire the best formulation of honey star fruit sorbet by adding sugar and lime juice and to find out the effect of different concentration of sugar and lime juice on the level of predilection, physical traits, level of vitamin C, and antioxidants activity. This study consisted of two phases; the production of lime essence in phase one and the making of star fruit sorbet in phase two as the main study. The result of best formulations selected based on organoleptic test of panellists’ level of predilection were formulation of 150 g sugar + 30 g lime, 90 g sugar + 60 g lime, and 120 g sugar + 60 g lime. Analysis of variance results indicated different formulation of star fruit sorbet had significant effect (P<0.05) on colour, flavour, aroma, and overall but showed no difference on the texture of star fruit sorbet. Overall, the best formulation was 150 g sugar + 30 g lime juice with overrun score of 23.52%, pH 4.20, sugar content of 26.85 oBrix, melting time of 43.16 minutes, vitamin C content of 0.968 mg, and antioxidant activity of 320.86 ppm.Keyword: antioxidant activity; hedonic test; organoleptic test.
A review on gene pyramiding of agronomic, biotic and abiotic traits in rice variety development Ibrahim Silas Akos; Mohd Rafii Yusop; Mohd Razi Ismail; Shairul Izan Ramlee; Norazyah Abd Aziz Shamsudin; Asfaliza Binti Ramli; Bello Sani Haliru; Muhammad Ismai'la; Samuel Chibuike Chukwu
International Journal of Applied Biology Vol. 3 No. 2 (2019): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v3i2.7818

Abstract

Rice Oryza sativa L is a staple food crop, and its seeds are the most important component part of the agronomic trait of the cereal crop, rich in nutrient and of economic value to human and even livestock. But, it is often threatened by various abiotic and biotic conditions that reduce the yield, because of high incidences of infectious disease agents and non-pathogenic conditions respectively. Pyramiding of the requisite resistance and tolerance genes into single elite high yielding variety of rice, confers wider spectrum of stress management, resulting to development of single multiline variety of rice. Marker-assisted selection utilizes DNA marker-linked primers for blast resistant gene (RM8225;Piz, RM6836;Piz, Pi2,Pi9), bacteria leaf blight (RM224; Xa-4, RM122;xa-5, RG136; xa-13, RM21;Xa-21) and drought tolerance (RM236;qDTY2.2, RM520;qDTY3.1, RM511;qDTY12.1) in pedigree, backcross and recurrent selection breeding methods. The objectives are to create awareness on the environmental safety of host-resistance, significance of single multiline resistance variety, effect of the interaction of stress conditions and associated simple sequence repeat (SSR) linked markers.
Performance evaluation of maize in Jumla district of Nepal: from yielding perspective Jiban Shrestha
International Journal of Applied Biology Vol. 3 No. 2 (2019): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v3i2.8060

Abstract

The performance evaluation of maize genotypes  at a specific growing region is a key to crop improvement. The objectives of this study were to evaluate grain yield of maize genotypes at Bijayanagar (Mahatgaun VDC) and Tallo Lorpa (Dillichaur VDC), Jumla District of Nepal during spring seasons of 2012 and 2013. The maize genotypes were significant (P< 0.05) for grain yield, days to tasseling and silking and  disease scoring where as anthesis-silking interval, plant height and ear height were non significant. The pooled analysis of the results showed that the highest grain yield was produced by Ganesh-1 (4830 kg/ha) followed by KKT-POP (4408 kg/ha), KKT-14 (3952 kg/ha) and MGU-08 (3913 kg/ha, respectively. From this study, Ganesh-1, KKT-POP, KKT-14 and MGU-08 could be preferable choice for cultivation in Jumla district of Nepal.
Performance evaluation of lentil and chickpea genotypes in Doti district of Nepal Hari Kumar Prasai
International Journal of Applied Biology Vol. 3 No. 2 (2019): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v3i2.8113

Abstract

Lentil and chickpea are important winter legumes of Nepal. The experiments were carried out to evaluate the agronomic performance of lentil and chickpea genotypes at research field of Regional Agricultural Research Station (RARS), Bhagetada, Doti, Nepal during winter seasons of 2012 and 2013. The 16 lentil genotypes were evaluated in both years whereas 20 and 14 genotypes of chickpea were evaluated in 2012 and 2013 respectively. The genotypes were evaluated in a randomized complete block design with three replications. The results showed that both lentil and chickpea genotypes indicated differences for their morphological traits namely plant height, flowering, maturity, pods/plant and grain yield. The combined analysis of trials over years showed that lentil genotype namely Black Masuro produced the highest grain yield (790 kg/ha) followed by LG 12 (746 kg/ha) and ILL 3111 (747 kg/ha), respectively. Similarly, chickpea genotypes namely ICCX 840508-31 produced the highest grain yield (953 kg/ha) followed by ICCX 840508-40 (911 kg/ha) and BG 372 (850 kg/ha), respectively. It is suggested that the superior genotypes derived from these experiments could be further evaluated in farmers’ fields  before making recommendation for general cultivation

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