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International Journal of Applied Biology
Published by Universitas Hasanuddin
ISSN : 25802410     EISSN : 25802119     DOI : -
Core Subject : Science, Agriculture, Social, Engineering,
Agriculture, Biological Sciences & Forestry Automotive Engineering Energy Environmental Science
International Journal of Applied Biology is an international, peer reviewed journal, publishing high-quality research. International Journal of Applied Biology only publishes manuscripts in English. Please see the journal's Focus & Scope for information about its Scope and peer-review policy. International Journal of Applied Biology is available in print and online version.
Arjuna Subject : -
Articles 168 Documents
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Ligation, Transformation and Characterization of Rv 1926c Mycobacterium tuberculosis to Escherichia coli JM 109 For Latent Tuberculosis Immunodiagnostic Rosana Agus
International Journal of Applied Biology Vol. 2 No. 2 (2018): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v2i2.5813

Abstract

Tuberculosis caused by Mycobacterium tuberculosis is the biggest infectious disease causing human death in the world. The main challenge in controlling tuberculosis is to quickly and accurately diagnose tuberculosis infection. Several kits have been produced to diagnose tuberculosis, but have different sensitivity and specificity. This shows that the kit is not yet ideal for diagnosing tuberculosis, so the search for candidates for specific antigens still needs to be done. One potential antigen is the Rv 1926c encoding MPT 63 protein. This protein is known to induce Th1 cells and produce IFN λ from PBMC cells of patients infected with tuberculosis. The purpose of this study was to clone the Rv 1926c from Mycobacterium tuberculosis as a tuberculosis immunodiagnostic kit. The method used is isolating Rv 1926c with PCR, ligation to pGEM-T vector and transformation to E.coli host cell JM 109. Clone characterization was carried out by PCR and migration analysis. The results obtained are the recombinant clones obtained have successfully inserted with the Rv 1926 c
Bioaccumulation Monitoring of Heavy Metal Chromium (Cr) in Fish Oreochromis mossambicus in The Floating Net Cultivation in Sidoarjo Krembangan River sonny kristianto
International Journal of Applied Biology Vol. 2 No. 2 (2018): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v2i2.5089

Abstract

ABSTRACTFloating Net is a simple alternative cultivation methods. The river there is a garden in the Krembangan District Sidarjoi which is used as a place of cultivation of  Floating net fish oreochromis mossambicus (Oreochromis mossambicus), on the other hand is where waste disposal by the local community and some industries . This research aims to know the accumulation of heavy metals chromium (Cr) in the flesh of fish oreochromis mossambicus (Oreochromis mossambicus) and find out the feasibility of fish consumption oreochromis mossambicus at floating net on the river Krembangan Sidoaarjo. This research are observatif by using Purposive Random Sampling techniques. test the content of heavy metal chromium (Cr) in the flesh of fish oreochromis mossambicus (Oreochromis mossambicus) method using AAS (Atomic Absorbtion Spectrofotometry). The data generated in the analysis in quantitative descriptive.  In this research show that the heavy metal content of chromium (Cr) in the flesh of fish oreochromis mossambicus highs station III of 0.52 mg/Kg; I station of 0.51 mg/Kg II station of 0.48 mg/Kg on a sampling sedangkang the morning, at night the highest value on the station III of 0.50 mg/Kg; I station of 0.46 mg/Kg and the II of 0.39 mg/Kg. Average deposits of heavy metal Cr on fish oreochromis mossambicus (o. mossambicus) is still under the raw quality of the already established by the Director-General of POM 1989 i.e. amounted to 2.5 mg/kg but fall into the category bioakumulasi medium, so that fish oreochromis mossambicus is still worthy of consumption, but need to watch out for, considering the Cr are toxic, carcinogenic, bioakumulatif and biomagnifikasi would potentially interfere with health.  
Identification of Pseudomonas fluorescens using different biochemical tests Bikram Nepali; Sabin Bhattarai; Jiban Shrestha
International Journal of Applied Biology Vol. 2 No. 2 (2018): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v2i2.5260

Abstract

Pseudomonas fluorescens not only enhances the plant growth but also controls the fungal pathogens by production of anti fungal metabolites. The objective of this experiment was to identify P. fluorescens using different biochemical tests. This research was carried out in Plant Pathology Laboratory at Agriculture and Forestry University (AFU), Rampur, Chitwan, Nepal. The result of this experiment indicated that P.  fluorescens gave positive result for Catalase test, Gelatin liquefaction, Fluorescent pigment and Oxidase test but negative result for starch hydrolysis test. The colony of P. fluorescens was maximum in maize seed than that of rice seed after two hours of inoculation, whereas higher number of colony was found in rice seed than that of maize seed after twenty four hours of seed inoculation. This findings is useful for identifying colony of P. fluorescens  per seed which is necessary for better seedling growth and effective biological control of pathogens.
Effect of seedling age, seeding density and nitrogen fertilizer on growth and grain yield of rice (Oryza sativa L.) Saroj Thapa; Khagendra Thapa; Jiban Shrestha; Amit Chaudhary
International Journal of Applied Biology Vol. 3 No. 1 (2019): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v3i1.6688

Abstract

Rice (Oryza sativa L.) is first staple crop of Nepal. The national average yield of rice is less than its potential yield, for which poor agronomic management has been reported as the critical factor. Among various agro-management practices seedling age, seeding density and nitrogen rates significantly affect the growth and yield of rice. The lower seeding density produces the taller plant, more effective tiller, lower sterility and higher grain yield. On the other hand, seedling of older age from higher seeding density gives the lowest yield. Transplanting younger seedling with low seeding density and application of recommended dose of nitrogen gives a higher yield. This article summarizes various effects of seedling age, seeding density and nitrogen rates on plant height, number of effective tillers, number of filled grains, thousand grain weight and grain yield of rice. This information may be useful for maize growers and researchers.
Molecular Identification of Cellulolytic Bacteria From Mangrove Sediment at Tin Minning Region In West Bangka Ardiansyah Kurniawan; Suci Puspita Sari; Euis Asriani; andi kurniawan; abu bakar sambah; asep awaludin prihanto
International Journal of Applied Biology Vol. 3 No. 1 (2019): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v3i1.5848

Abstract

Cellulose as an abundant source of glucose in Indonesia requires acceleration of decomposition utilizing cellulolytic bacteria.  Cellulolytic bacteria can be obtained from the isolation of mangrove organic matter, such as sediments. Muntok Sub-district is one of the regions with the most tin mining in West Bangka Regency also has mangroves in the coastal area. Exploration of cellulolytic bacteria in mangroves with different environmental characteristics encourages researchers to find new bacterial strains that produce cellulase enzymes with new properties. Thirteen isolates were successfully isolated from three locations. Tembelok mangrove sediments produced Seven bacterial isolates, Peltim Mangrove samples produced three isolates and from Sukal Mangrove three isolates were obtained. Seven isolates showed clear zones in the Lugol test and three isolates including were gram-positive bacteria. Molecular test with 16S rRNA analysis showed TBL1 isolate has 85% similar identity of  Vibrio parahaemolyticus strain HY3 and TBL2 isolate has 98% similar identity of Bacillus amyloliquefaciens strain HS8. Bacillus amyloliquefaciens potential to further study as cellulose degrading bacteria for feed ingredients.
Study of Several Stages of Maturity and Storage Temperature on Color Changes and Shelf Life of Mangosteen (Garcinia mangostana L.) Inanpi Hidayati Sumiasih; Roedhy Poerwanto; Darda Efendi
International Journal of Applied Biology Vol. 3 No. 1 (2019): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v3i1.5967

Abstract

Color and freshness of mangosteen are important characters as benchmarks for consumers in the selection and purchase of mangosteen in the market. Color, freshness and shelf life of mangosteen are affected by the stage of maturity at harvest and the correct storage temperature. Information about the correct maturity stage for harvesting and storage temperature of mangosteen are needed by the mangosteen farmers, local merchant, and exporters as an effort to maintain the quality of fresh product. The objective of this research was to study the effect of several maturity stage at harvest and storage temperature to mangosteen color changes and shelf life during storage. The research used Completely Randomized Design of two factors. The first factor was the fruit maturity stage at harvest consisting of: Maturity Stage 1, 2, 3 and 4. The second factor was storage temperature of 15 oC and room temperature. The result of harvesting mangosteen at Maturity Stage 1 could maintain skin color longer than at Maturity Stage 2, 3, and 4. Mangosteens that were harvested at Maturity Stage 1 and 2, combined with storage temperature of 15 oC could maintain fruit quality up to 30 days after harvest and could be used for export market. While harvesting at Maturity Stage 3 could maintain fruit quality up to 25 days after harvest and Maturity Stage 4 up to 20 days after harvest. Harvesting at Maturity Stage 4 followed by 15 oC storage temperature and all Maturity stages combined with room temperature storage could be used for local market.Keyword: fruit color; horticultural commodities; queen of tropical fruits; shelf life 
The Use Extract of Siam Leaf Eupatorium odoratum L. as Alternative Material In Lowering Blood Glucose Ulfah Nur Amaliah; Eva Johannes; Munif S Hasan; Elis Tambaru
International Journal of Applied Biology Vol. 3 No. 1 (2019): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v3i1.5868

Abstract

The use of medicine as oral hypoglycemic and insulin have some limitation, the treatment for diabetics needs more expensive cost relatively because it uses in the long term and can give the unwanted  side effect. At the recent time, one of the conducted efforts is by using the plant as alternative medicine. This research aims to know the potential of leaves’ extract and to know the effectiveness of siam weed leaves extract’s dosage which most effectivein lowering the level of blood glucose in mice which is induced by alloxan. On this research, anti-diabetic effect of siam weed leaves was observed to male mice which had been induced by alloxan until the level of blood glucose >200 mg/dl. The given treatment orally was conducted during seven days with three variations of dosage concentration of infused extract, they are 5%, 10% and 20 %. Furthermore, it was also used the comparison i.e. infused extract of cinnamon bark with 10 % concentration. Data analyse used SPSS 23.0 program with one way ANOVA then was continued by using Duncan test. The research result shows that the siam weed leaves extract with dosage concentration 5%, 10% and 20% can decrease blood glucose level on mice that have suffered hyperglycemia and most effective on 20%. Thus, it can be concluded that siam weed leaves extract have the potential in lowering blood glucose.
Inhibition of Juice Komba-Komba (Chromolaenaodorata) Leaf Against Growth of Bacteria Staphylococcus aureus Reni Yunus; Muhammad Hilam; Satya Darmayani
International Journal of Applied Biology Vol. 3 No. 1 (2019): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v3i1.6062

Abstract

Nowadays many plants can be used as traditional medicines to overcome various diseases including infections, because the use of traditional medicines is relatively safer compared to drugs derived from chemicals. One of the local plants in Southeast Sulawesi that has been empirically used as a medicine for wounds is a race of plants. Komba-komba leaves (Chromolaena odorata) containcompounds alkaloid, flavonoids, tannins, phenols, triterpenoids, saponins and steroids which have their respective functions in inhibiting the growth of microorganisms or antibacterial. This study aims to determine the inhibition of komba-komba leaf extract (Chromolaena odorata) on the growth ofbacteria Staphylococcus aureus. This type of research is experimental laboratories. The method used was agar diffusion with 5 treatment concentrations, namely komba-sheep leaf extract concentration (Chromolaena odorata) 20%, 40%, 60%, 80% and 100%, positive control (tetracycline) and negative control (aquadest) and repetition . The results showed that there was a inhibition zone of komba-komba leaf extract (Chromolaena odorata) on the growth ofbacteria Staphylococcus aureus at a concentration of 20% at 2 mm, a concentration of 40% at 3.7 mm, a concentration of 60% at 4.7 mm, a concentration of 80% 6.2 mm and a concentration of 100% by 8 mm.
The Potential of Sea Worm Perinereis aibuhitensis Extract as Anti-microbe toward Bacteria Salmonella typhi and Fungus Candida albicans Andi Evi Erviani; Asadi Abdullah; Nurwahida Nurwahida; Febriany Tairas
International Journal of Applied Biology Vol. 3 No. 1 (2019): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v3i1.5871

Abstract

The research about the potential of sea worm Perinereis aibuhitensis extract as anti-microbe toward bacteria Salmonella typhi and fungus Candida albicans had been conducted. The aim this research is to know the extract’s concentration of sea worm Perinereis aibuhitensis which is effective to obstruct the growth of bacteria Salmonella typhi and fungus Candida albicans. The result of research that was obtained shows that sea worm’s extract which used ethanol solution 96% and was needed in the culture of bacteria Salmonella typhi with the concentration of sea worm’s extract 7.5%, 15%, 30%, and 60% involved the form of obstruction zone at the medium with duration incubation 1x24 hours and 2x24 hours. Based on the research which was conducted, it can be concluded that the sea worm Perinereis aibuhitensis extract had the potential as anti-microbe toward bacteria Salmonella typhi  because can obstruct the growth of that bacteria, but it had no effect as anti-microbe toward fungus Candida albicans.
Lectin Protein Spodoptera litura Activity After Exposured by Biopesticide from Mirabilis jalapa Dina Maulina; Sutiman B Sumitro; Muhammad Amin; Sri Rahayu Lestari
International Journal of Applied Biology Vol. 3 No. 1 (2019): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v3i1.6080

Abstract

Spodoptera litura is folifagus agricultural pest with a tendency of resistance towards chemistry compound. This becomes a reason in the efforts to overcome it. The use of botanical material becomes one of the alternatives in controlling its population. The plant of Mirabilis jalapa has the active biopesticide compound that becomes its alternative prevention. This plant has the potential chemistry compound as biopesticide which works toward weakening immunity that is ended by the death of S. litura.  This research aims to analyze the existence of lectin protein as an indicator of immunity reaction’s activation Spodoptera litura after exposing biopesticide M. jalapa. Lectin test was conducted by using spot-test hemagglutination assay (HA) then was seen its speed forming of titer. The result of research shows that lectin was on the part of hemolimf S. litura supernatant. The result test shows that on the concentration 0.2% binding of lectin, carbohydrate and erythrocyte cells of vertebrate formed faster. The speed of titer forming was influenced by the number of hemosit. This is caused by immulectin receptors were on the cell’s surface. Therefore, exposing M. jalapa can induce lectin activation which functioned as the recognizing receptor of strange object which directly bound with carbohydrate related to the reaction of body immunity.Key words: biopesticide Mirabilis jalapa, lectin, immunity system, Spodoptera litura.

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