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Phytochemicals Screening, Minerals Composition and Proximate Analysis of Garlic (Allium sativum) Dauda, Maianguwa Abdulrsashid; Aliyu, Sulaiman Abubakar; Goje, Lazaru Joseph; Habibu, Bilyaminu; Joel, Emmanuel Askeb-nde; Umaru, Isaac John; Iseko, Kingsley Iyoko; Istifanus, Dafup Katdel
African Journal of Biochemistry and Molecular Biology Research Vol 1 No 1 (2024): African Journal of Biochemistry and Molecular Biology Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajbmbr.v1i1.3666

Garlic (Allium sativum) is a widely used medicinal plant with potential health benefits. The use of medicinal plants as remedies or medicine in prevention and treatment of several diseases was in place for many years. Garlic (Allium sativum) is among those plants because it possesses those characteristics. In this study, we investigated the phytochemical constituents, mineral composition, and proximate analysis of garlic. The qualitative phytochemicals screening of garlic ethanolic extract reveals the presence of Tannins, Alkaloid, Saponin, steroid, Glycosides, phenols and flavonoid while the quantitative shows that the extract composed of Alkaloid 10%, Saponin 5.30%, Tannin 4.10 µg/ml, Glycosides 4.40%, flavonoid 2.20%, phenols 3.14 µg/ml. The proximate analysis reveals that the garlic extract contains carbohydrate 65.84%, fiber 7.14%, fat 2.15%,ash 9.90%,moisture 7.58%, protein 7.30% The determination of some minerals composition in (mg/kg) reveals that the extract is composed of sodium(22mg/kg), potassium(531.5 mg/kg), zinc(7.1 mg/kg), copper(0.36 mg/kg), Iron(2.7 mg/kg), calcium(233 mg/kg) and magnesium(35.4 mg/kg) respectively were also detected, highlighting garlic's potential as a nutraceutical food supplement.. This research contributes valuable insights into the bioactive components of garlic, supporting its traditional use and potential health benefits. Further studies could explore its antioxidant, antimicrobial, and therapeutic properties.

Production and Characterisation of Peroxidase from Aspergillus terrens Isolated from Water Sample in Wukari, Taraba State, Nigeria Timothy, Mgbede; Abah, Moses Adondua; Ayo, Victoria Ifeoluwa; Ahmed, Maryam Usman; Oyibo, Okpanachi Nuhu; Ijeomah, Nkechi Gift; Gbadebo, Adetoyi Moses; A, Ajala-Lawal R.; Najeeb, Aliyu Olamilekan; Morolake, Olabanji Martina; Iseko, Kingsley Iyoko; Istifanus, Dafup Katdel; Umaru, Isaac John
African Journal of Biochemistry and Molecular Biology Research Vol 1 No 1 (2024): African Journal of Biochemistry and Molecular Biology Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajbmbr.v1i1.3667

The use of biocatalysts like enzymes in the development of environmentally sustainable processes has been reported as an ecofriendly approach for the current bioeconomy. This research aimed at producing and characterizing peroxidase from Aspergillus terrens isolated from water sample in Wukari, Taraba State, Nigeria. Fungi species from surface water sample obtained in Federal University Wukari, Taraba Nigeria were isolated through serial dilution of peptone water and was cultured in a room temperature in different petri dishes using Potato Dextrose Agar (PDA). A portion of the fungi growth chosen from the best growth of cultured water sample were cut and transferred into the 250 Erlenmeyer flask each containing an acceptable proportion of phosphate buffer, pH 6.0. The screening for peroxidase production was aseptically performed on a plate by inoculating the fermented medium with azur B dye. The appearance of a clear zone indicated a positive result for peroxidase activity. The incubation of the peroxidase enzyme was conducted over eleven days, with the highest enzyme yield observed on the eighth day. The results obtained in this study showed that the effect of pH on peroxidase activity was studied, revealing that the optimum pH for enzyme activity was 7.5. Peroxidase activity was lowest at pH 1. Temperature variation studies showed that the optimum temperature for peroxidase activity was 30°C. Peroxidase activity changed as substrate concentration was varied. Peroxidase activity was revealed to be highest at substrate concentration 1.9 mM. The kinetic parameters for peroxidase were investigated to be: Vmax = 10.57082; Km = 0.244186. The evidence presented in this research has shown that peroxidase can be produced in mass by using of white rot fungi from/around our surroundings and can be explored as a cheap source of peroxidase for industrial and biotechnological purposes. In conclusion, producing peroxidase from local isolates presents an eco-friendly and affordable alternative for industrial applications, contributing to environmental sustainability and public health.

Effect of Medicinal Plants on Liver and Malaria Pathogenesis Joel, Emmanuel Askeb-nde; Umaru, Isaac John; Iseko, Kingsley Iyoko; Istifanus, Dafup Katdel
African Journal of Biochemistry and Molecular Biology Research Vol 1 No 1 (2024): African Journal of Biochemistry and Molecular Biology Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajbmbr.v1i1.3674

This seminar reviewed the literatures on the effects of medicinal plants on liver and malaria pathogenesis. Malaria parasite has a complex life cycle that takes place both inside the mosquito and human beings. Generally, diagnosis of malaria is classified into clinical and parasitological diagnoses. Lack of clear understanding on the overall biology of Plasmodium (malaria), its life cycle and its mechanism of action has created a challenge in an effort to develop new drugs, and preventive methods against malaria such as using malaria vaccines and vector control. They have been a rise in the use of herbal supplements, natural products, and traditional medicines over the years. The use of herbal plants or their preparations in the management of various diseases including liver diseases has been practiced for several decades and its extension in current dispensation is recognized. It has been shown that the effect of medicinal plants is somehow related to belief, tradition and culture of the community. However, there are growing concerns related to the safety and toxicities of these medicines. These herbal medicines are associated with complications such as liver damage with a high incidence of mortalities and morbidities. Clinical manifestations range from asymptomatic cases with abnormal liver functions tests to sudden and severe liver failure necessitating liver transplantation. The liver is a very important organ with a lot of functions such as metabolism, detoxification, and storage of nutrients for the host to survive. Standard liver marker enzymes such as ALT, AST, ALP, albumin, globulin are essential when carrying out liver function tests. Medicinal plant components are essential for and can be beneficial or detrimental to the healthy or diseased liver. Medicinal Plants are an essential part of the human diet and comprise various compounds (photochemical) such as alkaloid (pyrrolizidine), kavalactone which are related to liver health. Selected medicinal plants can provide nutritional and medicinal support for liver diseases. At the present, the knowledge of the effects of medicinal plants on the liver is still incomplete. The most urgent task at the present time is to find the best dietary and medicinal plants for liver health in an endless list of candidates globally. This review updates the knowledge about the effects of medicinal plants consumption on the health of the liver, putting particular emphasis on the potential beneficial and harmful impact of medicinal plants on liver function.

Effects of Ethanolic Extracts of Fruits of Acacia nilotica and Flowers of Calotropis procera on Liver Function of Aspirin-Induced Male Albino Rats Ismail, Muhammad Zuhairah; Chinedu, Imo; Adebisi, Arowora Kayode; Philip, Shadrach; Mohammed, Rashida Ismail; Umaru, Isaac John; Iseko, Kingsley Iyoko; Istifanus, Dafup Katdel
African Journal of Biochemistry and Molecular Biology Research Vol 1 No 1 (2024): African Journal of Biochemistry and Molecular Biology Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajbmbr.v1i1.3675

This study examined the effects of ethanolic extracts of fruits of Acacia nilotica and flowers of Calotropis procera on the liver function of male albino rats. Acacia nilotica fruits and Calotropis procera flowers are commonly consumed by natives in northern Nigeria for therapeutic purposes. Eighty-four (84) healthy male albino rats were used for this study. The animals were randomly distributed into six groups of ten animals each, while 24 rats were used for lethal dose (LD50) analysis. The animals were administered ethanolic extracts of fruits of Acacia nilotica and flowers of Calotropis procera for 5 and 14 days respectively after induction with aspirin. The study was carried out in two phases: The animals were induced with 500 mg/kg body weight of aspirin 12 hours before the commencement of the experiment, the test animals were administered ethanolic extract of fruit of Acacia nilotica and flowers of Calotropis procera for 5 and 14 days and sacrificed at the end of each test phase respectively, blood was collected for biochemical analysis. The liver was harvested at the end of each phase, processed for histological investigation and photomicrographs taken. The result of liver function parameters showed that the mean values of alanine transaminase (ALT) after five-day of treatment decreased non-significantly (p>0.05) in groups 4, 5 and 6 and decreased significantly (p<0.05) in group 4 when compared to normal control. Also, ALT increased significantly (p<0.05) in group 2. There was a general decreasing trend in the mean values of aspartate transaminase (AST) across all groups except group 2. The mean values of alkaline phosphatase (ALP) decreased significantly (p<0.05) in all groups, but increased non-significantly in group 2. The same trend was observed in the values of ALT, AST, and ALP after 14 days of treatment. Photomicrograph of the liver section of normal rats showed normal central vein, sinusoids and hepatocytes as well as that of the treated animals. Photomicrograph of liver section of rat administered aspirin only showed slightly dilated portal triad after five days. The study suggests that the ethanolic extracts from fruits of Acacia nilotica and flowers of Calotropis procera may have active ingredients that are capable of improving some liver functions.

Analysis of Bioactive Constituents (HPLC) of Chloroform Leaf Extract from Kalanchoe pinnata in Takum, Taraba State Nigeria Joel, Emmanuel Askebnde; Wilson, Ubur Terzulum; Umaru, Isaac John; Iseko, Kingsley Iyoko; Istifanus, Dafup Katdel; Oladele, Gloria Omonefe
African Journal of Sciences and Traditional Medicine Vol 1 No 1 (2024): African Journal of Sciences and Traditional Medicine
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajstm.v1i1.3676

HPLC techniques were employed to analyze the bioactive components of the chloroform leaf extract of Kalanchoe pinnata leaf from Takum in Taraba State. A botanist from Taraba State University's Department of Biological Sciences in Jalingo identified the plant leaves, which were then gathered, cleaned, and processed. Using cold maceration, extraction was done by weighing 1500g of the powdered leaf into 1 liter of ethanol and distill water, respectively. Until further investigation, the extracts were stored in a refrigerator in a firmly closed container. The injection valve was used for the HPLC analysis, and the conditions were 20 μl, a UV variable wavelength detector (set at 300 nm), reprosol 100 C8mn, 5 μm 4.6 x 150mm column (30°C), and sensitivity of 0.001. Phosphate buffer (v/v) was used as an aqueous solvent (A) and CH3CN as an organic solvent (B) in HPLC. Gradient elution of the analytes occurred at a flow rate of 1 milliliter per minute. Software generated chromatograms. Operating temperature of the HPLC instrument was room temperature (23 ± 2°C). After injecting 20 μl of each diluted extract into the HPLC three times, the average peak areas were produced and utilized for quantification. Based on the HPLC analysis, the chloroform leaf extract of Kalanchoe pinnata contained the following bioactive constituents: quercetin, gallic acid, and chlorogenic acid. Additionally, the presence of vitamins B1 and B3 was also noted. The different bioactive constituents eluted at different retention times, and their respective amounts were also detected.

Analysis of Bioactive Constituents (HPLC) of Chloroform Leaf Extract from Kalanchoe pinnata in Takum, Taraba State Nigeria Joel, Emmanuel Askebnde; Wilson, Ubur Terzulum; Umaru, Isaac John; Iseko, Kingsley Iyoko; Istifanus, Dafup Katdel; Oladele, Gloria Omonefe
African Journal of Sciences and Traditional Medicine Vol 1 No 1 (2024): African Journal of Sciences and Traditional Medicine
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajstm.v1i1.3676

HPLC techniques were employed to analyze the bioactive components of the chloroform leaf extract of Kalanchoe pinnata leaf from Takum in Taraba State. A botanist from Taraba State University's Department of Biological Sciences in Jalingo identified the plant leaves, which were then gathered, cleaned, and processed. Using cold maceration, extraction was done by weighing 1500g of the powdered leaf into 1 liter of ethanol and distill water, respectively. Until further investigation, the extracts were stored in a refrigerator in a firmly closed container. The injection valve was used for the HPLC analysis, and the conditions were 20 μl, a UV variable wavelength detector (set at 300 nm), reprosol 100 C8mn, 5 μm 4.6 x 150mm column (30°C), and sensitivity of 0.001. Phosphate buffer (v/v) was used as an aqueous solvent (A) and CH3CN as an organic solvent (B) in HPLC. Gradient elution of the analytes occurred at a flow rate of 1 milliliter per minute. Software generated chromatograms. Operating temperature of the HPLC instrument was room temperature (23 ± 2°C). After injecting 20 μl of each diluted extract into the HPLC three times, the average peak areas were produced and utilized for quantification. Based on the HPLC analysis, the chloroform leaf extract of Kalanchoe pinnata contained the following bioactive constituents: quercetin, gallic acid, and chlorogenic acid. Additionally, the presence of vitamins B1 and B3 was also noted. The different bioactive constituents eluted at different retention times, and their respective amounts were also detected.

A Review: Biochemical Role of House Fly in the Transmission of Medically Important Parasites Joel, Emmanuel Askebnde; Alooma, Emmanuel; Wilson, Ubur Terzulum; Umaru, Isaac John; Iseko, Kingsley Iyoko; Istifanus, Dafup Katdel
Kwaghe International Journal of Sciences and Technology Vol 1 No 1 (2024): Kwaghe International Journal of Sciences and Technology
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/kijst.v1i1.3685

House flies (Musca domestica) can be found everywhere in the world except Antarctica, especially in regions with dense populations, hot weather, and inadequate hygiene. They are insects of small to medium size, featuring a unique gray body and big compound eyes that are colored red. House flies experience full metamorphosis, which includes four separate life phases: egg, larva (maggot), pupa, and adult. House flies are seen as important pests for public health because they can carry various pathogens, such as bacteria, viruses, protozoa, and parasites. Because of their close connection to human actions, capability to travel far distances, and inclination to breed in decaying organic material, they are highly effective carriers for transmitting different illnesses. House flies have been linked to the spread of more than 100 different disease-causing microorganisms, leading to illnesses like gastroenteritis, dysentery, and cholera. Apart from bacterial pathogens, house flies have also been discovered to play a role in spreading different viruses and medically significant parasites. This review describes the physical characteristics and behavior of the house fly, highlighting its unique attributes like compound eyes, antennae, thorax, abdomen, and wings. The stages of development of a house fly, from egg to larva, pupa, and adult. It also offers distinct ways in which house flies can spread medically significant parasites, emphasizing the public health impact of this occurrence, especially in regions with inadequate sanitation and restricted healthcare access. It is essential to comprehend the role of house flies in transmitting these parasites in order to create successful control methods and enhance public health results.

A Review: Biochemical Role of House Fly in the Transmission of Medically Important Parasites Joel, Emmanuel Askebnde; Alooma, Emmanuel; Wilson, Ubur Terzulum; Umaru, Isaac John; Iseko, Kingsley Iyoko; Istifanus, Dafup Katdel
Kwaghe International Journal of Sciences and Technology Vol 1 No 1 (2024): Kwaghe International Journal of Sciences and Technology
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/kijst.v1i1.3685

House flies (Musca domestica) can be found everywhere in the world except Antarctica, especially in regions with dense populations, hot weather, and inadequate hygiene. They are insects of small to medium size, featuring a unique gray body and big compound eyes that are colored red. House flies experience full metamorphosis, which includes four separate life phases: egg, larva (maggot), pupa, and adult. House flies are seen as important pests for public health because they can carry various pathogens, such as bacteria, viruses, protozoa, and parasites. Because of their close connection to human actions, capability to travel far distances, and inclination to breed in decaying organic material, they are highly effective carriers for transmitting different illnesses. House flies have been linked to the spread of more than 100 different disease-causing microorganisms, leading to illnesses like gastroenteritis, dysentery, and cholera. Apart from bacterial pathogens, house flies have also been discovered to play a role in spreading different viruses and medically significant parasites. This review describes the physical characteristics and behavior of the house fly, highlighting its unique attributes like compound eyes, antennae, thorax, abdomen, and wings. The stages of development of a house fly, from egg to larva, pupa, and adult. It also offers distinct ways in which house flies can spread medically significant parasites, emphasizing the public health impact of this occurrence, especially in regions with inadequate sanitation and restricted healthcare access. It is essential to comprehend the role of house flies in transmitting these parasites in order to create successful control methods and enhance public health results.

Burnout as a Predictor of Quality of Life Among the Federal Road Safety Corps Employees in Benue State Sector Command Zakariah, Danladi; Akafa, Vika Tensaba; Akafa, Tensaba Andes; Oladele, Gloria Omonefe; Iseko, Kingsley Iyoko; David, Izam Emmanuel; Ujah, Oche Williams
Kwaghe International Journal of Arts, Humanities and Religious Studies Vol 1 No 1 (2024): Kwaghe International Journal of Arts, Humanities and Religious Studies
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/kijahrs.v1i1.3983

This study investigated the relationship between burnout and quality of life among the Federal Road Safety Corps Employees in Benue State Sector Command. The study employed cross sectional and expost facto design where 200 participants consisting of 161 (80.5%) males, 37 (18.5%) females and 2 (1.0%) did not indicate their sex. Their ages ranged from 21-60 years with means age of 35.55 (SD=7.21). Maslach Burnout Inventory (MBI) and quality of Life Scale (WHOQOL) were used for data collection using Multistage, Stratified random and systematic sampling techniques. Statistical analysis involved the use of multiple regressions. Findings indicated that, there was a significant negative relationship between burnout and quality of life among the Federal Road Safety Corps Employees in Benue State Sector Command. The result of the finding reviewed that there was a significant negative relationship between burnout and the overall quality of life among the Federal Road Safety Corps employees in Benue State Command [R=.562, R2=.316, F (3,178)=27.376, p<.001]. This implies that burnout accounted for 31.6% of the total variance observed in quality of life among the Federal Road Safety Corps employees in Benue State Sector Command. The result of the study also showed the individual contributions of the various dimensions of burnout to overall quality of life which indicated that emotional exhaustion made the highest significant negative contribution (β=-.414, t=-5.180, p<.001) followed by depersonalization (β=-.235, t=-3.081, p<.01) while personal accomplishment made no significant contribution to the model (β=.027, t=.355, p>.05) respectively. Based on the findings of this study, it was recommended that conscious efforts should be made to enhance quality of life among the Federal Road Safety Corps Employees through reduction of burnout stress inherent in the work place among the Federal Road Safety Corps Employees in Benue State Sector Command and Nigeria at large.

Public Knowledge, Attitude And Acceptability of COVID-19 Vaccine among Traders in Wuse Market Abuja: A Cross-Sectional Study Akafa, Tensaba Andes; Oladele, Gloria Omonefe; Iseko, Kingsley Iyoko; Akafa, Vika Tensaba; David, Izam Emmanuel; Ujah, Oche Williams; John, Tongle Nanle; Nanmar, Chakfa; Tyem, Lawal Danjuma; Umaru, Isaac John
African Journal of Medicine, Surgery and Public Health Research Vol 1 No 1 (2024): African Journal of Medicine, Surgery and Public Health Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajmsphr.v1i1.3976

The effect of the COVID-19 pandemic on public health has been unpleasant. Vaccination is a critical measure to decrease COVID-19 economic loss and public health burden. This is very helpful to control the COVID-19 pandemic. The process of halting COVID-19 solely depends on population uptake of the vaccination process and the adoption of non-pharmacological measures. This in turn relies on the public willingness to get vaccinated. The public acceptability of this vaccination is further determined by the level of knowledge and trust in currently available COVID-19 vaccines. Therefore, this paper aims to discuss the findings of public knowledge, attitude, and acceptability of COVID-19 vaccines among traders in Wuse Market Abuja. A cross-sectional study was conducted among different traders in the Abuja Wuse Market to assess their knowledge, attitude and level of acceptability toward COVID-19 vaccinations using a self-administered survey questionnaire with the following four sections; socio-demographic characteristics, knowledge assessment questions, attitude assessment, and acceptability questions. A total of 166 participants completed the questionnaire. The knowledge of the COVID-19 vaccinations among the study participants was high with 143(86%) respondents stating that they knew about COVID-19 vaccinations. Among the traders that participated, 74(44.36%) believed that vaccines are safe and 109 (66.02%) agreed that vaccines are critical to protect the public from COVID-19 infection. But only, 45 (27.4%) accept to receive the vaccine. Participants’ attitudes, knowledge, and level of acceptability were significantly affected by age, gender, education level, and marital status (P <0.001). Our findings suggest that there is good knowledge and attitude toward the vaccination process against COVID-19 among study participants in spite of low acceptability. Awareness campaigns are therefore necessary to disseminate reliable knowledge about COVID-19 vaccines and demystify the COVID-19 conspiracy theory in order to raise the level of vaccination acceptance.

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