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POTENSI FITOKIMIA DAN AKTIVITAS ANTIMIKROBA DAUN SIRSAK (Annona Muricata Linn.) SEBAGAI KANDIDAT BAHAN PAKAN AYAM PEDAGING Londok, Jola J.M.R.; Mandey, Jet S.
JURNAL LPPM BIDANG SAINS DAN TEKNOLOGI Vol 1, No 1 (2014)
Publisher : JURNAL LPPM BIDANG SAINS DAN TEKNOLOGI

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Penelitian ini bertujuan untuk mempelajari potensi antibakteri daun sirsak sebagai kandidat bahan pakan ayam pedaging. Peneitian ini merupakan informasi awal dalam menentukan arah penggunaan daun sirsak. Penelitian dilakukan dengan menganalisis kandungan fitokimia meliputi analisis proksimat, analisis van Soest, dan analisis skrin fitokimia untuk melihat potensi daun sirsak yang ada di lokasi penelitian. Komposisi kimia tepung daun sirsak dalam penelitian ini yang diperoleh dari analisis proksimat dan Van Soest. Kandungan zat-zat makanan daun sirsak terdiri dari 87.58% bahan kering, 8.93% abu, 16.9% protein, 28.36% serat kasar, 4.76% lemak kasar, 28.63% Beta-N, 2.09% Ca, dan 0.35% P. Kandungan energi bruto sebesar 4195 kkal/g. Hasil analisis Van Soest menunjukkan bahwa dalam daun sirsak terdapat 53.96% NDF (neutral detergent in-soluble fiber), 49.61% ADF (acid detergent insoluble fiber), 4.08% hemiselulosa, 34.71% selulosa, 14.64 lignin dan 0.25 silika. Hasil skrining fitokimia kualitatif dengan pelarut etanol menunjukkan bahwa pada daun sirsak terdapat metabolit sekunder berupa steroid, flavonoid, tannin, dan saponin. Hasil penelitian menunjukkan bahwa daun sirsak dapat digunakan sebagai salah satu bahan penyusun ransum pada ternak ayam, dengan memperhatikan kandungan seratnya. Kandungan flavonoid dan saponin sebagai metabolit sekunder dapat diisolasi untuk dijadikan senyawa antioksidan potensial.Kata Kunci: Daun sirsak, Annona muricata Linn., fitokimia

Pengaruh Pembatasan pakan terhadap performa ayam pedaging strain yang berbeda Lantowa, Z.; Londok, J.J.M.R.; Imbar, M.R.
ZOOTEC Vol 41, No 1 (2021)
Publisher : Universitas Sam Ratulangi

THE EFFECT OF FEED RESTRICTION ON THE PERFORMANCE OF BROILERWITH DIFFERENT STRAINS. This study aims to measure the growth performance of broilers with different strains through a feed restriction pattern at the beginning of growth (starter). This study used 72 day-old broilers consisting of 36 Cobb strains and 36 Lohman strains selected from each of them. -each 100 heads to get almost the same body weight obtained successively from PT. Charoen Phokphand and PT. Japfa Comfeed Indonesia. The design used was a completely randomized design (CRD) with a 2x4 factorial pattern with 3 replications. The A factor is the difference in strains (A1 = Lohman, A2 = Cobb), while the B factor is the time of feed restriction (B0 = no feed restriction, B1 = feed restriction starts on day 8, B2 = Feed restriction starts on day 11, B3 = Feed restriction begins On day 14. There were 8 treatment combinations. Feed restriction was carried out as much as 20 percent. The measured variables were feed consumption, body weight gain, and feed conversion. The results of the analysis of variance showed that there was a significant interaction (P <0.05) between the strains different from the start of the feed restriction on feed consumption and body weight gain, while the feed conversion variable did not show a significant interaction (P> 0.05) .Tukey's test results showed that the feed consumption in the combination of Lohman strain treatment with limitation time of 11 days (98.95) is not significantly different (P> 0.05) with the combination of A1B0 (97.52), A2B3 (91.34), but A2B0 (95.36) is significantly different (P <0.05) is higher with all combinations treatment. The results of the analysis of variance showed that there was a significant interaction (P <0.05) between factor A and factor B. The results of the Tukey test showed that the treatment of A1B2 (54.63) was not significantly different (P> 0.05) with A1B1 (51, 70), thus with treatment A2B0 (48.13), treatment A1B0 (49.93) with A2B1 (49.13), A2B3 (49.12), A2B0 (48.13) and A2B2 (47.21) . Lohman strain broiler and 20% restriction with restriction time starting on the eleventh day gave the best performance response.Â Keywords: Strain, feed restriction, time limitation

POTONGAN KOMERSIAL KARKAS AYAM BROILER STRAIN COBB YANG MENGALAMI PEMBATASAN PAKAN DAN PEMBERIAN SUMBER SERAT KASAR BERBEDA PADA PERIODE GROWER Tumiran, Megawati; Rompis, John E. G.; Mandey, Jet S.; Nangoy, Fredy J.; Londok, Jola J.M.R.
ZOOTEC Vol 39, No 1 (2019)
Publisher : Universitas Sam Ratulangi

Study was conducted to evaluate the effect of feed restriction and source of different crude fiber on carcass weight and commercial pieces of broiler with Cobb strain. Study was using 24 broilers with Cobb Strain. Restriction of 20 percent feed and different sources of crude fiber were applied to broilers at age of 21to 28 days old. Ration was fed ad libitum to broilers until birds reached age of42 days old. . This research was using a Completely Randomized Design (CRD) in Factorial pattern of 2x4 with 3 replications. As a factor A was restricted feeding consisted of without restrictions (A0), and 20 percents restricted ration (A1). A factor B was source of crude fiber consisted of commercial feed (B0), commercial feed with coffee hull meal (B1), commercial feed with rice bran (B2), and commercial feed with coconut pulp (B3). There were treatment combinations including A0B0, A0B1, A0B2, A0B3, A1B0, A1B1, A1B2 and A1B3. The variables measured were percentages of commercial pieces consist of breast, thigh, wing and brisket. The data analyzed used variance analysis. The honestly significance difference test was used to determine which one of treatment was significantly different from each other. The results showed that the combination treatment of feed restriction and source of crude fiber in feed gave a very significant different effect (P <0.05) on carcass weight of broiler with Cobb strain. Sources of fiber crude affected significantly (P<0.05) percentages of thigh, breast, brisket and live weight. Treatment of feed restriction affected significantly (P<0.05) percentage of wing. Restriction of 20 percents with coconut pulp as source of crude fiber produced better commercial carcass of broilers withCobb strain.Â Keywords: Feed restriction, crude fiber source, broiler commercial pieces, Cobb strain

PENGARUH PEMUPUKAN ANORGANIK DAN ORGANIK TERHADAP PRODUKSI TANAMAN JAGUNG SEBAGAI SUMBER PAKAN. Dewanto, Frobel G.; Londok, Jola J.M.R.; Tuturoong, Ronny A.V.; Kaunang, Wilhelmina B.
ZOOTEC Vol 32, No 5 (2013): Edisi Januari
Publisher : Universitas Sam Ratulangi

Suatu penelitian telah dilakukan untuk melihat sejauh mana pengaruh pemupukan anorganik dan organik terhadap tinggi tanaman, panjang dan lingkar tongkol serta bobot pipilan kering jemur jagung. Penelitian ini dilaksanakan di lahan pertanian milik keluarga M. Manoppo yang dimulai sejak bulan April 2009 sampai dengan Oktober 2009 di Desa Koka Kecamatan Tombulu Kabupaten Minahasa.Â Jumlah petak yang digunakan dalam penelitian ini sebanyak 20 (dua puluh) petak yang ditempatkan secara acak dengan ukuran 2 x 2 meter dengan jarak tanam 50 cm x 70 cm dan jenis benih jagung yang digunakan adalah jagung hibrida (NUSANTARA 1). Rancangan penelitian yang digunakan adalah Rancangan Acak Kelompok (RAK), terdiri dari 5 kelompok dan 4 perlakuan yang terdiri dari T0 (tanpa pemupukan), T1 (pemupukan anorganik dengan dosis 3,67 kg/petak), T2 (pemupukan organik dengan dosis 1,4 kg/petak) dan T3 (menggabungkan pupuk anorganik dengan dosis 1,83 kg/petak dan pupuk organik dosis 0,7 kg/petak). Variabel yang diukur meliputi tinggi tanaman, panjang dan lingkar tongkol serta bobot pipilan kering jemur. Pengambilan data dilakukan disaat panen sampai dengan selesai penjemuran jagung yang telah dipipil. Data yang diambil kemudian ditabulasi dan diuji menurut analisis keragaman untuk melihat pengaruh perlakuan. Uji lanjut Beda Nyata Jujur (BNJ) dilakukan untuk melihat perbedaan antara masing-masing perlakuan. Hasil analisis keragaman menunjukkan bahwa perlakuan memberi pengaruh yang berbeda nyata (P > 0,05) terhadap panjang dan lingkar tongkol serta bobot pipilan kering jemur. Dari penelitian yang telah dilakukan diambil kesimpulan bahwa pemupukan dengan menggabungkan antara pupuk anorganik dan organik lebih meningkatkan produksi tanaman jagung baik itu panjang tongkol, lingkar tongkol dan bobot pipilan kering jemur.

PENGARUH PEMBERIAN SUMBER SERAT BERBEDA DALAM PAKAN TERHADAP KECERNAAN SEMU SERAT KASAR DAN HEMISELULOSA PADA AYAM PEDAGING STRAIN COBB Sasae, Yasinta Y.A.; Londok, J.J.M.R.; Tulung, B.; Rahasia, C.A.
ZOOTEC Vol 40, No 1 (2020)
Publisher : Universitas Sam Ratulangi

THE EFFECT OF DIFFERENT FIBER SOURCES IN THE FEED TO DIGESTIBILITY OF CRUDE FIBER AND HEMICELLULOSE IN COBB STRAIN OF BROILERS. This research was carried out with the aim to determine the effect of providing different sources of fiber in feed on the apparent digestibility of crude fiber and hemicellulose in cobb strain of broilers. This study used 20 cobb strain of broilers 35-day-old. The design used was a Completely Randomized Design (CRD) with the treatment of 4 different fiber sources in the feed which was repeated 5 times. The treatments consisted of: R0 (commercial feed),R1 (commercial feed with coffe skin), R2 (commercial feed with rice bran), R3 (commercial feed with coconut pilp). Variables measured were: apparent digestibility of crude fiber and hemicellulose. Apparent-digestibility measurements using the total collection method. Data were analyzed using general linear model (MINITAB version 16), then differences between treatments were tested with Tukeyâ€™s simultaneous test. Apparent digestibility of crude fiber feed has a very different (P<0,01) with treatment R2 and R1. The treatment of R2 was not significantly different (P>0,05) from R1. Apparent digestibility test for hemicellulose shows that R3 is the same asÂ R0, but it is significantly different (P<0,01) with treatment R2 and R1. The treatment od R2 was not significantly different (P>0,05) from the treatment of R1. It was concluded that coconut pilp as a source of fiber in the feed of strain cobb of broilers provides better digestibility of coarse and hemicellulose fibers than coffe huks and coffe branKeywords: Apparent digestion of crude fiber, Apparent digestion of hemicellulose, strain cobb of broiler

KUALITAS KARKAS AYAM PEDAGING YANG DIBERI RANSUM MENGANDUNG LIMBAH SAWI Londok, Jola J.M.R.; Rompis, John E.G.; Mangelep, Claudya
ZOOTEC Vol 37, No 1 (2017)
Publisher : Universitas Sam Ratulangi

ABSTRACT CARCASS QUALITY OF BROILERS FED RATION CONTAINING WASTE OF SAWI. Study was conducted to evaluate the effect of substituting part of ration with sawi waste meal on carcass quality and abdominal fat of broilers. Total of sixty unsexed day old chick broilers of MB-202 P strain were randomly divided into twenty units of pens, each of pen was filled with three heads of chicks.Â Birds were maintained during forty days. Levels of substituting part of ration with sawi waste meal wereÂ 0, 5, 10, 15, and 20 percents. Each treatment had four replications using completely randomized design with 5 x 4. Fresh water and ration were given ad libitum. Birds from day old chick to the age of twenty one days old were fed with BR-21E ration for all treatments. The treatments of ration were fed at ages of twenty two days old to the end of study (period when all animals were slaughtered). Data were analyzed using F test and Tukey test for the significant F test. Results showed that control treatments were significantly different (P<0.05) with those others four treatments for carcass weight, but not significantly different with those others four treatments for carcass percentages and abdominal fat. Therefore, sawi waste meal can be used to substitute part of ration up to 20 percents for broilers ration in term of the carcass percentages and abdominal fat. Â Key words: Sawi waste meal, broilers, carcass quality.

Antioxidant Enzyme Activity and Malondialdehyde Concentration on Broiler Fed Contain Lauric Acid and Areca vestiaria Giseke Jola Josephien Mariane Roosje Londok; Sumiati Sumiati; I Komang Gede Wiryawan; Wasmen Manalu
Buletin Peternakan Vol 42, No 2 (2018): BULETIN PETERNAKAN VOL. 42 (2) MAY 2018
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v42i2.31767

The objective of the study was to determine antioxidant enzyme activity and concentration of malondialdehyde on broiler which consumed feed containing lauric acid and natural antioxidant from Areca vestiaria Giseke. The study used 240 day-old chicks of Lohmann MB 202 P strain placed randomly at 24 experimental cage units (10 chickens each) of the litter system. The average temperature of the cage at the study was 25.80 to 32.080 C with relative air humidity rate of 67.69 to 86.20%. The research method followed a Factorial Completely Randomized Design 2 x 4 with 3 replications. The first factor was the source of lauric acid in feed, i.e. conventional coconut oil (CO) and pure lauric acid (LA). The second factor was the source of antioxidant in the form of dosage of Areca vestiaria (AV) and vitamin E supplementation (TF) with four levels of AV, ie 0, 625 mg.kg-1, 1250 mg.kg-1, and TF at a dose of 200 mg .kg-1 feed. Feed and drinking water were given ad libitum. Meat sampling to measure SOD, catalase and MDA was taken at the end of the study by first chickens were fasted for ± 8 hours (overnight). The sample used was right breast meat of experimental male chicken. The data were average from three experimental chickens. The data obtained were analyzed for the variant with the general linear model on MINITAB (version 16), then tested the differences between treatments using Tukey's honestly significant difference (HSD). The results showed that the source of laurate had a significant effect on SOD, catalase, and MDA, while antioxidant concentration significantly decreased SOD and MDA compared with control. The interaction between lauric sources and antioxidant concentrations affected catalase and MDA. The use of coconut oil as much as 3% and antioxidant supplementation of Areca vestiaria Giseke in feed at a dose of 1250 mg kg-1 could decrease lipid oxidation product of meat to produce a healthy food product.

Profil Hematologi Ayam Pedaging yang Diberi Ransum Mengandung Asam Laurat dan Pinang Yaki Sebagai Sumber Antioksidan Alami (HAEMATOLOGY PROFILE OF BROILER FED LAURIC ACID AND ARECA VESTIARIA GISEKE AS A SOURCE OF NATURAL ANTIOXIDANT) Jola Josephien Mariane Roosje Londok; Wasmen Manalu; I Komang Gde Wiryawan; Sumiati Sumiati
Jurnal Veteriner Vol 19 No 2 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The objectives of the present study were to investigate the effect of dietary coconut oil as a source of lauric acid and Areca vestiaria Giseke (AV) as a natural antioxidant source on some haematological profile. Two hundred and forty (240) day-old unsexed Lohmann broiler chicks (MB-202 P) were divided into twenty four experimental units (ten chicks per unit) and arranged in a completely randomized design with a 2 x 4 factorial arrangement. Each experimental unit was repeated 3 times each with ten chicks. The first factor was the source of lauric acid in the ration consisted of 2 levels i.e., coconut oil and pure lauric acid. The second factor was dose of antioxidant consisted of 4 levels i.e., 0 (without antioxidant [AV and lauric acid] supplementation), AV at a dose of 625 mg kg-1 ration, AV at a dose of 1250 mg kg-1 ration, and tocopherol at a dose of 200 ppm). Blood sampling were collected from chicken at the end of experiment period (age 35 days). Total number of erythrocyte and leukocyte, Hb, PCV, together with absolute count of deferentiation of leukocyte were determined. General linear model and Tukey test were used for statistical analysis of the result. Blood sampling were collected from chicken at the end of experiment period (age 35 days). Total number of erythrocyte and leukocyte, Hb, PCV, together with absolute count of deferentiation of leukocyte were determined. General linear model and Tukey test were used for statistical analysis of the result. The study showed that source of lauric acid, antioxidant concentration and its interaction gave the same effect (P>0.05) to the erythrocytes, leucocytes, Hb, PCV, MCH, MCHC, and monocyte percentage of the broiler. The MCV in chickens consuming pure lauric acid was significantly higher than that of coconut oil. MCV values in chickens that received antioxidant AV at a dose of 625 mg kg-1 were significantly higher (P<0.05) than the others. The source of lauric acid was significant (P<0.01) affecting the percentage of lymphocytes and eosinophils, whereas the concentrations of antioxidants significantly affected the percentage of lymphocytes and heterophils, as well as the apparent effect (P<0.05) indicated by the eosinophil and basophil percentages.. The results of this study indicate that the use of lauric acid and concentration of antioxidant AV in the ration does not change the health status of broiler.

Kecernaan in vitro pakan broiler yang mengandung level asam laurat dan serat kasar berbeda Y.H.S. Kowel; A. Bagiu; J.J.M.R. Londok
ZOOTEC Vol. 42 No. 1 (2022)
Publisher : Universitas Sam Ratulangi

Penelitian kecernaan mengunakan metode in vitro dengan pakan yang mengandung level asam laurat dan serat kasar berbeda untuk mengukur tingkat kecernaan bahan kering dan bahan organik pakan. Penelitian ini menggunakan rancangan acak lengkap (RAL) pola faktorial 3x2x4. Faktor A adalah level asam laurat yaitu A1 = 1,3%; A2 = 1,95%; dan A3 = 2,6%. Faktor B adalah level serat kasar yaitu B1 = 6% dan B2 = 8%. Setiap kombinasi perlakuan diulang 4 kali. Parameter yang diukur adalah kecernaan bahan kering dan kecernaan bahan organik tahap 2 (pepsin digestibility), serta kadar air dan kadar abu pakan. Hasil analisis keragaman menunjukkan bahwa perlakuan memberikan pengaruh berbeda sangat nyata (P<0,01) terhadap kecernaan bahan kering (36,48% - 41,87%) dan kecernaan bahan organik (33,21% - 38,69%) serta kadar air (11,98% - 13,66%) dan kadar abu pakan (8,09% - 11,30%). Peningkatan pemberian level asam laurat memberikan tingkat kecernaan yang lebih baik ini disebabkan karena mempunyai bobot molekul yang lebih kecil sehingga tidak diperlukan energi yang tinggi dan hanya membutuhkan sedikit enzim untuk memecah lemak tersebut menjadi bentuk yang siap diserap. Berdasarkan hasil penelitan yang telah dilakukan dapat ditarik kesimpulan penggunaan 2,6% level asam laurat dan 6% serat kasar memberikan kecernaan bahan kering dan bahan organik secara in vitro yang lebih baik.Â Kata Kunci: Asam laurat, serat kasar, kecernaan

Kecernaan bahan kering dan bahan organik ransum broiler yang ditambahkan mananoligosakarida (MOS) berasal dari ampas kelapa J. Suak; J.J.M.R. Londok; Y.H.S. Kowel
ZOOTEC Vol. 43 No. 2 (2023)
Publisher : Universitas Sam Ratulangi

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Penelitian ini dilaksanakan dengan tujuan untuk mengetahui kecernaan bahan kering dan bahan organik ransum broiler yang ditambahkan manan oligosakarida (MOS) berasal dari ampas kelapa. Penelitian ini menggunakan 20 ekor ayam pedaging strain CP707 berumur 35 hari. Metode percobaan menggunakan rancangan acak lengkap (RAL), dengan 5 perlakuan 4 ulangan, Sebagai perlakuan adalah level ektraksi MOS (0, 1, 2, 3 dan 4%). Setiap perlakuan diulang 4 kali. Peubah yang di ukur yaitu konsumsi bahan kering ransum, kecernaan bahan kering, dan kecernaan bahan organik. Data analisis menggunakan Minitab versi 16. Hasil analisis ragam menunjukkan bahwa perlakuan berbeda tidak nyata terhadap nilai konsumsi dan kecernaan bahan kering ransum, sedangkan untuk kecernaan bahan organik menunjukkan perbedaan yang nyata antar perlakuan. Uji Tukey menunjukan R0 berbeda nyata dengan R1, tapi berbeda tidak nyata dengan R2, R3, dan R4. R1 berbeda nyata dengan R2 dan R4, tapi berbeda tidak nyata dengan R3. R2 berbeda tidak nyata dengan R3 dan R4. Disimpulkan bahwa penambahan ampas kelapa sebagai sumber manan oligosakarida (MOS) sebanyak 1% dalam ransum broiler tidak mengubah konsumsi dan kecernaan bahan kering namun memberikan nilai kecernaan bahan organik terbaik. Kata kunci: kecernaan bahan kering, kecernaan bahan organik, ampas kelapa, manan oligosakarida. ABSTRACT Digestibility of Dry Matter and Organic Matter of Broiler Feed Added Coconut Pulp as a Source of Mannan Oligosaccharides (MOS). This study was conducted with the aim of determining the digestibility of dry matter and organic matter in broiler feeds added coconut pulp as a source of mannan oligosaccharides (MOS). This study used 20 broiler chickens CP707 strain aged 35 days. The experimental method used a completely randomized design (CRD), with 5 treatments and 4 replications. The treatments were level of MOS extracted (0, 1, 2, 3, and 4%). Each treatment was repeated 4 times. The variables measured included dry matter consumption of the ration, dry matter digestibility, and organic matter digestibility. Data ware analyzed using Minitab version 16. The results of the analysis of variance showed that the treatment was not significantly different on the consumption value of dry matter rations and digestibility of dry matter, but for digestibility of organic matter showed significantly different. Tukey’s test that R0 was significantly different from R1, but not significantly different from R2, R3, and R4. R1 is significantly different from R3. R2 is not significantly different from R3 and R4. It was concluded that the addition of coconut pulp as a source of 1% mannan oligosaccharide (MOS) in broiler rations did not change the consumption and digestibility of dry matter but gave the best organic matter digestibility values. Keywords: dry matter digestibility, organic matter digestibility, coconut pulp, mannan oligosaccharides.

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