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Journal : e-Journal Pustaka Kesehatan

Efek Stresor Rasa Sakit Renjatan Listrik terhadap Limfosit dan Makrofag pada Gingiva Tikus Sprague Dawley (The Effect of Electrical Shock Stressor on Lymphocytes and Macrophages in Gingival Tissue of Sprague Dawley Rats) Vananda Duanta Kantasa; Banun Kusumawardani; Herniyati .
Pustaka Kesehatan Vol 4 No 1 (2016)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Background: Stress has negative impact for oral health and can cause periodontal disease, because the secretion of cortisol increase when stress occur. The excessive secretion of cortisol assumed to decrease the number of lymphocytes and macrophages in gingiva which play a role on immunity of oral health. Objective: To determine the effect of electrical shock stressor on the number of lymphocytes and macrophages in gingival tissue of Sprague Dawley rats. Methods: Sprague Dawley rats were given electrical shock for 7 days (S1), 14 days (S2), and 28 days (S3). Lymphocytes and macrophages observed on histological preparation in interdental gingiva between first molar and second molar used binocular microscope, 1000x magnification. Result: The results showed that the number of lymphocytes in S1 group (3,67±0,816), S2 (3,83±0,752), and S3 (3,17±0,527), also macrophages in S1 group (0,83±0,752), S2 (1,00±0,894), and S3 (0,67±0,816) were lower than the number of lymphocytes (0,67±0,816) and macrophages (2,00±0,632) in control group. The number of lymphocytes (p = 0,002) and macrophages (p = 0,033 ) had significant difference between groups. Conclusion: The electrical shock stressor decreased the number of lymphocytes and macrophages in gingival tissue of Sprague Dawley rats that could cause the oral cavity risk to periodontal infection.   Keywords: electrical shock stressor, gingiva, lymphocytes, macrophages, stress
Efek Induksi Gaya Mekanis Ortodonti Terhadap Perubahan Jumlah Sel Osteoblas Tulang Alveolar Gigi Tikus Pada Daerah Tarikan Reganita Nurmaulawati Saputri; Herniyati Herniyati; Dwi Prijatmoko
Pustaka Kesehatan Vol 9 No 2 (2021): Volume 9 No.2, 2021
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v9i2.20035

Abstract

Orthodontic treatment is one treatment that can improve the dental malocclusion. Bone remodeling involves the coordination of three cell types namely osteocytes, osteoclasts, and osteoblasts. Osteoblast cells is important in the process of bone remodeling, especially in the process of bone aposition in tension side. This study aimed to analyze the changes in number of osteoblasts cell in tension side of Sprague dawley alveolar bone which induced by orthodontic force during one weeks, two weeks, and three weeks. 36 rats were divided into 6 groups, the treatment group with apllied the orthodontic force for 1 weeks (P-1), 2 weeks (P-2), and 3 weeks (P-3) and thr control group without apllied the orthodontics force group for 1 weeks (K-1), 2 weeks (K-2), and 3 weeks (K-3). Wire ligature 0,20 mm was apllied on the right region of maxillary first Molar and Incicivus, then mesially activated with maximally tension gauge of Orthodontic appliance of Ni-Ti closed coil springs. Those samples were sacrified and sliced vertically on the regio right maxillary bone with mesiodistal position, then the preparation of histological section with Hematoxilyn Eosin staining (HE) was made. The number of osteoblast cells were observed in 3 areas of tension side using microscope with 400 magnification. The result showed that the osteoblasts number of 2- and 3-weeks treatment groups were greater significantly than control group. It concluded that the induction of orthodontic appliance mechanic increased the number of osteoblast on tension area. The increasing of osteoblast number is paralel with the duration of the orthodontic appliance mechanic induction.
Efek Seduhan Kopi Robusta terhadap Jumlah Limfosit pada Daerah Tarikan Ligamen Periodontal Gigi Marmut (Cavia Cobaya) yang Diinduksi Gaya Mekanis Ortodonti Ginanjar Hidayatullah; Herniyati Herniyati; Leliana Sandra Deviade Putri
Pustaka Kesehatan Vol 7 No 3 (2019): Volume 7 No.3, 2019
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v7i3.11560

Abstract

Malocclusion is an occlusion that deviates from the normal state, so it needs to be treated using an orthodontic device. The formation of new alveolar bone after obtaining an orthodontic force will be surrounded by osteoid bones are not stable enough (relapse). Robusta coffee (Coffea robusta) has flavonoids which can increase lymphocyte proliferation. The purpose of this study was to analyze the effect of robusta coffee on the number of lymphocyte cells in the tension area of marmot’s (Cavia cobaya) periodontal ligament which induced by mechanical orthodontic forces. This type of research is laboratory experimental with the Post Test Only Control Group Design. The average number of lymphocyte cells in group A = 0.75, B = 1.45, C = 1.26, D = 1.54. One-way Anova test results showed that the number of lymphocyte cells in all groups showed a significant difference (p <0.05). The results of the post hoc LSD test showed that group A had significant differences with group B and group D (p <0.05). While group C did not have a significant difference with group A (p> 0.05). The conclusion obtained is that the provision of robusta coffee brew for 2 weeks can increase the number of lymphocyte cells in the tension area of marmot’s (Cavia cobaya) periodontal ligament which induced by orthodontic mechanical forces compared to without robusta coffee brew.
Perbedaan Kekuatan Tarik Bahan Adhesif Total-Etchdengan Bahan Adhesif Self-Etchpada BondingBraket Ortodonsi (The Difference of Tensile Strength Between Total-Etch Adhesive Material and Self-Etch Adhesive Material for Orthodontic Bracket Bonding) Dita Nur Ekasari; Herniyati .; Dwi Prijatmoko
Pustaka Kesehatan Vol 2 No 3 (2014)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Background.Orthodontic treatment using a bracket attached to the teeth dentofasial aiming to get a good look aesthetically. At the beginning of orthodontic bracket bonding systems to enamel surface through several stages of work so that they can spend time, includes teeth cleaning, basting phosphoric acid etching, washing with water, drying, basting material bonding / primer, and application of composite resin as a bracket adhesive material called total-etch technique. But as the times have found self-etch adhesive that further simplify the time and phases of work, the incorporation of phosphoric acid etching and bonding materials / primer in one package. Tensile strength is the strong adhesive tensile or the ability of an object to hold while receiving an attractive force and the force coming from the direction perpendicular to the surface of the object, for example when there is an attractive force toward the labial or buccal tooth driven. Purpose. This study aimed to determine differences in tensile strength and know the best adhesive that can be used between total-etch adhesive with a self-etch adhesive on orthodontic bracket bonding. Methods.This study used 16 pieces mandibular firstpremolars were divided into two groups, each consisting of eight teeth. The first group used a total-etch adhesive, while the latter uses self-etch adhesive. Bracket adhesive tensile strength was measured using Torsee's Digital System Universal Testing Machine. Results.Independent t-test showed a significant difference between groups in total-etch adhesive with a self-etch adhesive (p <0.05). Conclusions and suggestions.There is a significant difference in tensile strength measurements of total-etch adhesive with adhesive and self-etch adhesive total-etch proved better than a self-etch adhesive on orthodontic bracket bonding. Further research needs to be done with respect to the composite resin material used, the stages of research, and tools used tensiletest. Keywords:bonding, self-etch adhesive, tensile strength, total-etch adhesive
Prevalensi Kebiasaan Buruk Sebagai Etiologi Maloklusi Klas I Angle Pada Pasien Klinik Ortodonsia RSGM Universitas Jember Tahun 2015-2016 Arimbi Gupitasari; Herniyati .; Leliana Sandra Devi Ade Putri
Pustaka Kesehatan Vol 6 No 2 (2018)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v6i2.8660

Abstract

Habit is a repetitive act that is done spontaneously and commonly occurs in childhood. A habit in the oral cavity that can cause malocclusion is called a bad habit. Bad habits can affect the dentofacial functions such as the process of chewing, talking, tooth occlusion, periodontal tissue, and esthetic. If bad habits continue after six years old, they can cause malocclusion. The etiology of malocclusions can be classified into general and local factors. Bad habit is one of the general factors that play a role in the occurrence of malocclusion. Kinds of bad habits are sucking the thumb/finger, pushing the tongue, biting the lips and nails, wrong swallowing habits, breathing through the mouth, and bruxism. The study investigate the prevalence of bad habit and bad habit that mostly happened as etiology of Angle’s Class I malocclusion in patient of Orthodontic Clinic RSGM Jember University. This descriptional study with total sampling technique was conducted by identifying dental records and study models of Angle’s Class I malocclusion that caused by bad habit. Obtained data were tabulated and analysed so that conclusion could be made. Prevalence of Angle’s Class I malocclusion caused by bad habits was 9.4% with the largest distribution in thumb/finger sucking (38,7%), in the age group 9-10 years (50%), and in the male gender (53,6%). Keywords: Prevalence of bad habits, etiology of Angle’s Class I malocclusion
Ekspresi BMP2 dan TRAF6 pada Jaringan Periodontal setelah Pemberian Ekstrak Kopi Robusta selama Pergerakan Gigi Ortodonti Herniyati Herniyati; Leliana Sabdra deviade Putri; Hestieyonini Hadnyanawati; Happy Harmono
Pustaka Kesehatan Vol 11 No 2 (2023): Volume 11 No.2, 2023
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v11i2.38029

Abstract

Orthodontic tooth movement occurs due to mechanical stimulation followed by alveolar bone and periodontal ligament remodeling. Bone remodeling is the process of bone resorption in areas of tension and bone formation in areas of tension. Various techniques and drugs are used to speed up orthodontic treatment. This study aims to analyze the expression of BMP2 and TRAP6 on orthodontic tooth movement after the administration of Robusta coffee extract. Twenty-four Wistar rats were divided into 4 groups: C1 (2 weeks) and C2 (3 weeks) groups: rats were given orthodontic mechanical force (OMF) and T1 (2 weeks) and T2 (3 weeks) groups rats were given OMF and Robusta coffee extract. OMF in rats was performed by moving the maxillary right permanent molars mesially with a Niti closed coil spring. Observations were made on the 15th and 22nd day by immunohistochemical examination to determine the expression of BMP2 and TRAP6. Administration of Robusta coffee extract for 2 weeks and 3 weeks increased BMP2 and TRAP6 expression (p <0.05). Administration of Robusta coffee extract increased the expression of BMP2 and TRAP6 in the alveolar bone during orthodontic tooth movement.