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All Journal Journal of Natural Sciences and Mathematics Research Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) AGROMIX BUANA SAINS Journal of Food Technology and Agroindustry JURNAL ILMIAH AGRINECA Jurnal Abdiraja Fruitset Sains : Jurnal Pertanian Agroteknologi Jurnal Manajemen Agribisnis dan Agroindustri
Helilusiatiningsih, Nunuk
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Application of POC fertilization from organic waste and humic acid on the growth and production of sweet corn (Zea mays L. Saccharata sturt) Helilusiatiningsih, Nunuk; Syahara, Muhammad Alwi
AGROMIX Vol 15 No 2 (2024)
Publisher : Fakultas Pertanian Universitas Yudharta Pasuruan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35891/agx.v15i2.4434

Abstract

Introduction: Sweet corn in Indonesia was the main commodity after rice, and it was useful for food, the snack processing industry, semi-finished ingredients, side dishes, and animal feed. Problems with sweet corn farming include limited land, inappropriate cultivation technology, expensive superior seeds, insufficient water availability, fertilizers and pesticides that do not meet needs, as well as expensive and difficult labor, and competition with other commodities. The quality of the corn was that the husk was bright green and slightly moist, the sweet corn tassels were light brown and moist, and the grain texture was dense. The research aimed to analyze the effect of adding humic acid and POC on the growth and yield of sweet corn. Methods: The experimental design was RAKF. The first factor was humic acid (A) consisting of 4 levels: control, 5 kg/ha, 10 kg/ha, and 15 kg/ha. The second factor is POC (P) consisting of 5 levels: control, 60 ml/l, 90 ml/l, 120 ml/l, and 150 ml/l so there are 20 experimental combinations. Analysis used the F count test, 5% BNT test, and 5% DMRT test. Results: In the analysis of plant height, number of leaves, and weight of corn with and without husks, the best combination was A3P4.The P4 level (150 ml/L) has the highest level of sweetness but is not significantly different, the degree of sweetness was 16.25 brix. Addition of humic acid at a concentration of 15 kg/Ha. Produces a sweetness degree of 18.35 brix. Conclusion: The results of the study indicated a significant influence from the combination of treatments, as well as an interaction between the experimental treatments. The most effective combination was A3P4, which involved a humic acid concentration of 15 kg/ha and a POC dose of 150 ml/L. This combination yielded the best results for the tested parameters and sweetness levels.
Characterization and Antioxidant Activity Assay of Essential Oil Parts of Rosella (Hibiscus sabdariffa L.) Helilusiatiningsih, Nunuk; Syahara, Muhammad Alwi; Irawati, Titik
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 13 No. 3 (2024): September 2024
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v13i3.671-678

Abstract

Rosella (Hibiscus sabdariffa L.) is known as a plant whose calyx contain high levels of antioxidant bioactive compounds and good inhibitory power against free radicals. However, other parts of the rosella plant have not yet been utilized for their antioxidant content and activity. The aim of this research is to compare the profiles of essential oils of parts of the rosella plant (seeds, leaves and flower petals) and their antioxidant activity. This research method begins with the process of extracting essential oils using the water-steam distillation method. Furthermore, the profile of the essential oil is known from the results of yield calculations and characterization using the GC-MS instrument. The antioxidant activity was tested using the DPPH method. The yield of essential oils obtained was 0.0107% for seeds, 0.0087% for leaves and 0.0136% for flower petals. GC-MS characterization shows that the most abundant chemical components contained in the essential oils of seeds, leaves, and flower petals are fatty acid compounds (2-propanoic acid, butanoic acid, hexanoic acid) and esters (neopentyl isobutyrate). The results of the antioxidant activity test showed the IC50 value for each essential oil, namely, seeds: 30.15 μg/ml, leaves: 171.27 μg/ml and flower petals: 126.58 μg/ml. The compound characteristics obtained showed the best results for rosella seed essential oil, this was supported by the fairly high antioxidant activity test results IC50 <50 μg/ml. Keywords: Antioxidant activity, Characterization, Essential oils, IC50, Yield.
Antioxidant activity assay of Roselle (Hibiscus sabdariffa) seeds Ethanol extract with DPPH radical scavenging using UV-Vis Spectrophotometer Syahara, Muhammad Alwi; Helilusiatiningsih, Nunuk; Irawati, Titik
Journal of Natural Sciences and Mathematics Research Vol. 10 No. 1 (2024): June
Publisher : Faculty of Science and Technology, Universitas Islam Negeri Walisongo Semarang

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Abstract

Hibiscus sabdariffa (Roselle) is known to have a strong antioxidant compound. Thus, the calyx becomes the most important area to research. Another part of this plant that was starting to be known for its benefits is rosella seeds. Roselle seeds are a source of fat-soluble antioxidants. The aim of this study was to investigate the antioxidant activity of Roselle Seed Ethanol Extract (RSEE) with the DPPH (1,1-Diphenyl-2 Picrylhidrazyl) radical scavenging method. This research begins by doing the extraction of Roselle seeds using ethanol (96%) as a solvent and then assessing the antioxidant activity (Radical Scavenging Activity/RSA) of the extract, which is compared with the ascorbic acid standard at several concentrations (5-200 ppm). Antioxidant activity was assessed with DPPH Radical Scavenging using UV-Vis spectrophotometer and IC50 value parameters. The results of this study were the absorbance of DPPH from spectrophotometer at 517 nm was 0,824 (purple color). The absorbance of RSEE and ascorbic acid at 5 ppm to 200 ppm, respectively 0.523-0.124 and 0.594-0.112. The antioxidant activity (RSA) of the RSEE and ascorbic acid, respectively was  57.93% and 59.24%. The IC50 value of RSEE was 30.158 μg/ml and as comparison is ascorbic acid, the IC50 value was 26.948 μg/ml. Thus, the antioxidant activity of roselle seeds ethanol extract (RSEE) belongs to a very strong category (<50 μg /ml). This is due to the presence of several phenolic compounds and unsaturated fatty acid derivatives.
Co-Authors Adji, Galih Edy Soenyoto Endro Puji Astoko FITRIYAH, NUR Haliza, Nadiyah Nur Indis, Nadhifah Al Irawati, Titik Mardiana, Yushi Muhammad Alwi Syahara, Muhammad Alwi Nastiti Winahyu Naufa Anwar, Diva Nunuk Helilusiatiningsih Purbowo, Muhammad Lukman RIBUT SANTOSA Rosanti, Aulia Dewi Sani, Muhammad Yulius Setyo Utomo, Pamuji