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All Journal Jurnal Al Azhar Indonesia Seri Ilmu Sosial Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Exhibition and Seminar on Science and Creative Technology – Al Azhar Proceeding
Puspitasari, Riris Lindiawati
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Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Electrical & Electronics Engineering Environmental Science Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Social Sciences

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Comparison Of Liquid Luria Broth Media and Molasses Media on Recombinant L-Arabinose Isomerase Enzyme Production From Geobacillus Stearothermophilus Maulida, Aulia; Puspitasari, Riris Lindiawati; Noriko, Nita
EXSACT-A Vol 2, No 1 (2024)
Publisher : Universitas Al Azhar Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36722/exc.v2i1.2374

Abstract

L-Arabinose isomerase (L-AI) is a type of intracellular enzyme catalyzes the reversible isomerization reaction of L-arabinose to L-ribulose, besides that it can convert D-galactose to D-tagatose. One of the bacteria that can produce this enzyme is Geobacillus stearothermophilus. The high price of instant media, which reaches IDR 1,500,000 to IDR 3.000.000,- for every 500 g and the abundance of natural resources that can be used as a medium for the growth of microorganisms encourages researchers to find alternative media from materials that are easy to get and do not require expensive costs. The researchers compared the effectiveness of liquid Luria Broth (LB) media and molasses media for enzyme production. The research involved inoculating bacteria in both media types, followed by subculture and incubation. IPTG was added to induce enzyme production, followed by cell harvesting through centrifugation and sonication. Enzyme activity was evaluated using SDS PAGE and the cysteine carbazole method. Molasses showed promising results due to water solubility, enzyme activity, abundance, and cost-effectiveness. Molasses appears as an alternative with active enzymes, water solubility, abundant availability, and significant cost savings, making it a viable choice for enzyme production.Keywords— L- Arabinose Isomerase, Molasses, Enzyme
Evaluasi Program Merdeka Belajar Kampus Merdeka Mandiri Prodi Biologi Universitas Al Azhar Indonesia: Studi Kasus Respon Mahasiswa Wijihastuti, Risa Swandari; Perdana, Analekta Tiara; Pambudi, Arief; Alamsyah, Firman; Puspitasari, Riris Lindiawati; Effendi, Yunus; Fauzia, Nurul; Dianita, Adinda Citra
Jurnal Al Azhar Indonesia Seri Ilmu Sosial Vol 3, No 2 (2022): Juni 2022
Publisher : Universitas Al Azhar Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36722/jaiss.v3i2.1030

Abstract

Merdeka Belajar Kampus Merdeka (MBKM) merupakan salah satu program yang dilakukan pemerintah untuk meningkatkan kualitas pendidikan di Indonesia. Program tersebut terdiri dari 8 kegiatan yang dapat mengasah kompetensi hard skills maupun soft skills mahasiswa. Beberapa kegiatan Program MBKM telah dilakukan di Program Studi Biologi Universitas Al-Azhar Indonesia (Prodi Biologi UAI), yaitu program pertukaran pelajar, program asistensi mengajar dan program magang bersertifikat. Penelitian ini bertujuan untuk menjelaskan hasil evaluasi respons mahasiswa terhadap Program MBKM mandiri Prodi Biologi UAI. Penelitian dilakukan dengan metode kuantitatif menggunakan survey untuk mengetahui respons dari mahasiswa baik yang belum dan sudah melaksanakan Program MBKM. Hasil evaluasi respons mahasiswa menunjukan bahwa mahasiswa Prodi Biologi UAI sering mendengar istilah MBKM dan mengetahui bahwa Prodi Biologi UAI menyelenggarakan Program MBKM. Sebagian mahasiswa Prodi Biologi UAI yang belum mengikuti Program MBKM sangat berminat untuk bergabung, dan mahasiswa yang telah mengikuti Program MBKM merasa sangat puas dan ingin mengikuti program lainnya.Kata Kunci – MBKM, Prodi Biologi Universita Alzhar Indonesia, Respon
Cortisol Analysis of Pterygoplichthys pardalis from Ciliwung River Zhafirah, Adienda Yoesmah; Puspitasari, Riris Lindiawati
Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) 2020: Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (261.615 KB)

Abstract

The existence of Pterygoplichthys pardalis as one Loricariidae species were introduced to Indonesia and dominated the Ciliwung River. The high contaminant in Ciliwung River can lead to high stress levels so that fish experienced a decline due to the death or failure to reproduce. But not entirely external stressors affect the existence Pterygoplichthys pardalis. P. pardalis stress levels need to determined the adaptive mechanism polluted environment through the concentration of the hormone cortisol. This study was conducted to identify the level of stress P. pardalis based on concentration of the hormone cortisol. The method used a sample of fish raised in tank and fish from the river. Fish plasma will be analyzed using the ELISA method measure the cortisol. The higher concertration of cortisol showed more stress in Ciliwung fish. Based on the statistic result showed the significance value between two groups was 0.012 (sig<0.05). This assumed were difference cortisollevel between fish from tank and river. The results show that P. pardalis collected from river have higher concentrations of the cortisol than from tank. The cortisol level was 113.06 ng / ml.
Cortisol Analysis of Pterygoplichthys pardalis from Ciliwung River Zhafirah, Adienda Yoesmah; Puspitasari, Riris Lindiawati
Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) 2020: Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (260.539 KB)

Abstract

The existence of Pterygoplichthys pardalis as one Loricariidae species were introduced to Indonesia and dominated the Ciliwung River. The high contaminant in Ciliwung River can lead to high stress levels so that fish experienced a decline due to the death or failure to reproduce. But not entirely external stressors affect the existence Pterygoplichthys pardalis. P. pardalis stress levels need to determined the adaptive mechanism polluted environment through the concentration of the hormone cortisol. This study was conducted to identify the level of stress P. pardalis based on concentration of the hormone cortisol. The method used a sample of fish raised in tank and fish from the river. Fish plasma will be analyzed using the ELISA method measure the cortisol. The higher concertration of cortisol showed more stress in Ciliwung fish. Based on the statistic result showed the significance value between two groups was 0.012 (sig<0.05). This assumed were difference cortisollevel between fish from tank and river. The results show that P. pardalis collected from river have higher concentrations of the cortisol than from tank. The cortisol level was 113.06 ng / ml.
Co-Authors Arief Pambudi Dianita, Adinda Citra Fauzia, Nurul Firman Alamsyah Maulida, Aulia Nita Noriko Perdana, Analekta Tiara Wijihastuti, Risa Swandari Yunus Effendi Zhafirah, Adienda Yoesmah