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All Journal Jurnal Ilmu dan Teknologi Kelautan Tropis Bawal : Widya Riset Perikanan Tangkap Journal of Fisheries & Marine
Wulandari, Diah Anggraini
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Library & Information Science

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Effect of Ultrasonic Assisted Extraction with Ethanol for Removing Lipid on Catfish (Pangasius sp.) Skin as a Collagen Source and Its Characteristics Agustina, Maulida; Patmawati, Patmawati; Mubarok, Shofy; Sulmartiwi, Laksmi; Wulandari, Diah Anggraini; Zai, Khadijah; Siva, Raseetha; Pujiastuti, Dwi Yuli; Nirmala, Dwitha; Carattri Kusuma Werdani, Money; Moechthar, Oemar; Alwi Husein, Mohamad Akmal
Jurnal Ilmiah Perikanan dan Kelautan Vol. 16 No. 1 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v16i1.46061

Abstract

Abstract Fish skin is rich in collagen (80%-90%). However, catfish (Pangasius sp.) skin has a high lipid content, which can reduce the quality of collagen. Therefore, treatment is needed to remove the lipid using ultrasonic assisted extraction (UAE) with ethanol. Hence, the aim of the present study was to remove lipid content from catfish skin as a raw material for collagen by using UAE with different concentrations of ethanol (25, 50, and 75%). The research was conducted in three stages: removing impurities, collagen extraction, and characterization of collagen. The result exhibited that UAE-ethanol treatment was capable of removing up to 85.6% of lipid content, and the produced collagen had the potential to be utilized as an alternative source of collagen based on its properties. Highlight Research Ethanol could be used as extractor of lipid from catfish skin of Pangasius Ultrasonic assisted extraction could increase efficiency of lipid extraction from catfish skin. Fish skin of Pangasius sp. has a potential source for collagen. Collagen from skin of Pangasius sp. has a good characteristic with high protein content.
DIVERSITAS MOLUSKA DI PANTAI PAMEUNGPEUK, GARUT SELATAN, JAWA BARAT Mudjiono; Safaat, Muhammad; Sugara, Ayub; Wulandari, Diah Anggraini
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 14 No. 1 (2022): Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jitkt.v14i1.34718

Abstract

Moluska memiliki cara unik dalam mempertahankan dirinya pada daerah intertidal, selain itu moluska di daerah ini memiliki keanekaragaman yang berbeda dibandingkan zona lainnya. Oleh karena itu, perlu kajian mendalam terkait keanekaragaman dan cara adaptasi moluska pada daerah tersebut. Tujuan dari penelitian ini adalah untuk mengetahui jenis dan keanekaragaman moluska, cara adaptasi moluska pada gelombang tinggi, potensi ekonomis moluska, serta menentukan klaster komunitas moluska di Pantai Pamengpeuk, Jawa Barat. Penelitian ini berlokasi di Pantai Bubujung, Pantai Karang Paranje, Pantai Santolo Indah, Pantai Karang Papak, Pantai Taman Manalusu, Pantai Cicalobak, Pantai Karang Wangi, Pantai Ranca Buaya 2 dan Pantai Ranca Buaya 1 dengan menggunakan metode purposive sampling. Hasil pengamatan ditemukan 37 spesies moluska yang terdiri dari 33 jenis gastropoda dan 4 jenis bivalvia. Moluska di daerah ini beradaptasi dengan cara bersembunyi pada bebatuan dan mencari daerah yang lembab untuk mempertahankan kadar air di dalam tubuhnya. Famili dengan jumlah individu dan penyebaran tertinggi adalah Nodilittorina, Trochoides, dan Littorinidae. Indeks keanekaragaman (H') moluska tertinggi yaitu Pantai Ranca Buaya 1 dengan indeks keanekaragaman 2,8, sedangkan indeks kemerataan tertinggi (e) yaitu Pantai Parenje (0,92). Moluska yang berpotensi ekonomis untuk dikembangkan di Pantai Pamengpeuk, Garut Selatan antara lain Planaxis sulcata, Nerita polita, Nerita picea, Cerithium eburneum, dan Turbo setosus. Pantai Pemengpeuk memiliki tingkat keanekaragaman yang tinggi sehingga berpotensi untuk dikembangkan dalam rangka meningkatkan kelestarian dan perekonomian masyarakat di wilayah tersebut.
PENGGUNAAN DNA BARCODING DALAM MENGIDENTIFIKASI LARVA GASTROPODA (FAMILY CYMATIIDAE) DI PERAIRAN KEPULAUAN SANGIHE- TALAUD, SULAWESI UTARA Simbolon, Anna Rejeki; Ompi, Medy; Widyastuti, Ernawati; Wulandari, Diah Anggraini
BAWAL Widya Riset Perikanan Tangkap Vol 13, No 3 (2021): (DESEMBER) 2021
Publisher : Politeknik Kelautan dan Perikanan Sorong

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/bawal.13.3.2021.%p

Abstract

Perairan Kepulauan Sangihe-Talaud merupakan wilayah pemijahan hewan laut, sehingga berbagai jenis larva hewan laut dapat ditemukan di wilayah ini. Identifikasi larva gastropoda secara morfologi sangat sulit dilakukan karena tingginya kesamaan morfologi antar spesies pada fase larva. DNA Barcoding dapat digunakan untuk mengidentifikasi larva berbagai hewan laut. Penelitian ini bertujuan untuk mengidentifikasi larva gastropoda dari Family Cymatiidae di Perairan Kepulauan Sangihe-Talaud. Sampel larva diambil dengan menggunakan alat Issaac Kid Midwater Tramp (IKMT) di kedalaman 1000 m di bawah permukaan laut pada siang dan malam hari. Barcoding DNA menggunakan primer universal LCO 1490 dan HCO 2198, pengeditan dan alignment sekuen DNA dalam Geneious ver. 9 dan identifikasi menggunakan BLAST di GenBank NCBI. Pohon filogenetik dikonstruksi menggunakan metode neighbor joining (NJ) dan model Kimura-2i dalam MEGA X. Sebanyak 39 spesimen larva Cymatiidae terdiri atas 6 genus yaitu Reticutriton, Monoplex, Turritriton, Cymatium, Gutturnium, dan Ranularia; lima spesies teridentifikasi: Reticutriton pfeifferianus, Monoplex aquatilus, Monoplex comptus, Cymatium cingulatum dan Gutturnium muricinum, dengan tingkat kesamaan 97,32-100%. Berdasarkan pohon filogenetik sebagian sekuen individu termasuk kelompok monofiletik sedangkan genus Monoplex bersifat polifiletik dan mengelompok dalam genus yang berbeda dengan nilai boostrap yang cukup tinggi. Jarak genetik antar spesies berkisar 0,0761-0,1737 dengan jarak genetik antar spesies terendah pada Monoplex comptus dan Reticutricon pfeifferianus. Penelitian ini menyimpulkan DNA barcoding dapat digunakan dalam mengidentifikasi larva Gastropoda khususnya family Cymatiidae. Identifikasi jenis hewan laut secara dini menjadi salah satu kunci penting dalam pengelolaan lestari jenis hewan laut. Identifikasi larva yang tepat akan berguna dalam pengelolaan konservasi suatu wilayah perairan.The seas around the Sangihe-Talaud Islands are a spawning ground for marine animals, so many marine animals' larva can be found in this area. Morphological identification of gastropod larvae is complicated because of the high morphological similarities between species in the larval stage. DNA Barcoding can be used to identify the larvae of various marine animals. This study aims to identify gastropod larvae, especially the family Cymatiidae, in the waters around the Sangihe-Talaud Islands. Larvae samples were collected using an Isaac Kid Midwater Tramp (IKMT) at a depth of 1000m below sea level day and night. DNA barcoding used the universal primers LCO 1490 and HCO 2198. DNA sequences were edited and aligned in Geneious ver nine and identified using the NCBI GenBank BLAST routine. A phylogenetic tree was constructed using the neighbor-joining (NJ) method with the Kimura-2 model. 39 Cymatiidae larvae were identified as belonging to 6 genera: Reticutriton, Monoplex, Turritriton, Cymatium, Gutturnium, and Ranularia; five species were identified: Reticutriton pfeifferianus, Monoplex aquariums, Monoplex computers, Cymatium cingulated and Gutturnium muricinum, with a similarity 97.32-100%. Based on the phylogenetic tree, some of the individual sequences belong to monophyletic groups, while the Monoplex genus is polyphyletic and grouped into several genera with relatively high bootstrap values. The genetic distance between species ranged from 0.0761-to 0.1737 with the lowest inter-species genetic distance between Monoplex computers and Reticutricon pfeifferianus. This study concludes that DNA barcoding can be used to identify gastropod larvae, in particular the family Cymatiidae. Early identification of marine animal species is an important key to the sustainable management of marine animals. Identification of gastropod larvae can support the conservation management of marine waters.
Co-Authors Alwi Husein, Mohamad Akmal Anna Rejeki Simbolon, Anna Rejeki Ayub Sugara Carattri Kusuma Werdani, Money Dwi Yuli Pujiastuti Dwitha Nirmala Ernawati Widyastuti, Ernawati Maulida Agustina Medy Ompi Mubarok, Shofy Mudjiono N. Juni Triastuti Oemar Moechthar Patmawati Patmawati, Patmawati Safaat, Muhammad Siva, Raseetha zai, Khadijah