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All Journal Jurnal Sain Veteriner Jurnal Kedokteran Hewan Jurnal Veteriner dan Biomedis Current Biomedicine
Mayasari, Ni Luh Putu Ika
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Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Journal : Current Biomedicine

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Hipertrofi sel goblet usus halus ayam kampung asal pasar tradisional Jakarta yang terinfeksi cacing cestoda Jerica, Monica Silva; Tiuria, Risa; Mayasari, Ni Luh Putu Ika; Nugraha, Arifin Budiman; Subangkit, Mawar
Current Biomedicine Vol. 2 No. 2 (2024): July
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.2.2.93-100

Abstract

Latar Belakang: Ayam kampung merupakan salah satu kebutuhan protein hewani yang sering dicari masyarakat. Pemeliharaan ayam kampung dengan sistem dibiarkan lepas menjadi predisposisi terinfeksi oleh parasit gastrointestinal. Tujuan: Penelitian ini bertujuan mengkaji perubahan sel epitel dan hipertrofi sel goblet pada saluran pencernaan ayam kampung yang terinfeksi cacing cestoda Raillietina spp. secara alami. Metode: Penelitian ini menggunakan tujuh preparat arsip histopatologi usus halus ayam kampung yang terinfeksi cacing cestoda. Sampel usus halus ayam kampung diambil dari dua pasar yang berbeda, yaitu pasar Pluit di Jakarta Utara dan pasar Kebayoran Lama di Jakarta Selatan. Hasil: Hasil pengamatan histopatologis pada preparat usus ditemukan deskuamasi epitel vili dan proliferasi sel-sel kripta yang diakibatkan oleh infeksi cacing cestoda. Sel goblet yang mengalami hipertrofi paling banyak ditemukan pada sampel preparat yang diambil dari pasar Kebayoran Lama, Jakarta Selatan (p>0,05). Simpulan: Dapat disimpulkan bahwa deskuamasi vili dan jumlah hipertrofi sel goblet terjadi akibat infeksi cacing cestoda pada mukosa usus halus ayam kampung.
Dieffenbachia seguine extract nanoemulsion as an intranasal inflammation-inducing agent in rats Sa'diah, Siti; Leong , Ke Yong; Mayasari, Ni Luh Putu Ika; Widi, Leliana Nugrahaning; Alfarisi, Hamzah
Current Biomedicine Vol. 3 No. 2 (2025): July
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.3.2.94

Abstract

Background Dieffenbachia seguine (Jacq.) Schott, commonly known as the dumb cane, is a tropical ornamental plant known for its toxic and proinflammatory properties. Its potential as a natural inducer of inflammation warrants further investigation, particularly via the intranasal route. Objective This study aimed to evaluate the proinflammatory effect of D. seguine leaf extract nanoemulsion by measuring the levels of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) in bronchoalveolar lavage fluid (BALF) following intranasal administration in rats. Methods Wistar rats (n= 24) were divided into six groups: water for injection (WFI, negative control); emulsion base; lipopolysaccharide (LPS, positive control); and D. seguine extract nanoemulsions at concentrations of 1%, 2%, and 4%. Each rat received an intranasal instillation, and IL-6 and TNF-α concentrations in the BALF were quantified using ELISA. Results Only the 4% D. seguine nanoemulsion group showed a significant increase in IL-6 and TNF-α levels compared to the control. Lower concentrations (1% and 2%) did not induce significant cytokine elevation. All treatment groups showed significantly lower cytokine levels than the LPS group. Conclusion D. seguine extract nanoemulsion at 4% concentration demonstrates potential as a natural inflammation-inducing agent via the intranasal route, although its effect remains lower than that of LPS.
Co-Authors Alfarisi, Hamzah Andriyanto Assadah, Nur Syabiq Atik Ratnawati Ellis Dwi Ayuningsih Faisal Faisal I wayan Teguh Wibawan Indrawati Sendow Jerica, Monica Silva Leong , Ke Yong NI LUH PUTU INDI DHARMAYANTI Nugraha, Arifin Budiman Nuradji, Harimurti Pamungkas, Joko Pamungkas Panggabean, Ruben Hasiholan Prayitno, Lilik Putri, Shafa Adela Risa Tiuria RISZA HARTAWAN sa'diah, siti Subangkit, Mawar UUS SAEPULOH Widi, Leliana Nugrahaning