Auliya Ilmiawati
Department Of Chemistry, Faculty Of Mathematics And Natural Sciences, IPB University, Bogor 16680, Indonesia. Tropical Biopharmaca Research Center-Institute Of Research And Community Services, IPB University, Bogor 16128, Indonesia

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Antioxidant Capacity, Phytochemical Profile, and Clustering of Pomegranate (Punica granatum L.) Peel Extracts Using Different Solvent Extraction Rafi, Mohamad; Wulansari, Laela; Septaningsih, Dewi Anggraini; Purnomo, Tsania Firqin; Auliatifani, Reza; Khaydanur, Khaydanur; Ilmiawati, Auliya; Yulianti, Wina; Nengsih, Nunuk Kurniati; Suparto, Irma Herawati; Kusuma, Wisnu Ananta
Journal of Tropical Life Science Vol 11, No 3 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Pomegranate has valuable nutritional content and contains various bioactive compounds, one found in the fruit's peel. The utilization of these bioactive compounds could be used as herbal medicines and supplements, such as antioxidants. This study aimed to determine the antioxidant capacity, phytochemical profile, and pomegranate peel extract grouping using different extracting solvents. The three extracting solvents used were water, 70% ethanol, and ethanol p.a. Antioxidant capacity of the three extracts was measured using the DPPH and CUPRAC methods. We also determined the total phenolic and flavonoid levels and the TLC fingerprint analysis and FTIR spectrum of the pomegranate peel extracts. The 70% ethanol extract owned the largest antioxidant capacity than the other two extracts with a value of 358.67 and 2981.59 µmol trolox/g dried sample using the DPPH and CUPRAC methods, respectively. The three pomegranate peel extracts' total phenolic and flavonoid levels ranged from 287.26–1068.81 mg GAE/g dried sample and 0.24-0.75 mg QE/g dried sample. TLC fingerprint analysis of pomegranate peel extract yielded 2, 6, and 6 bands for water extract, 70% ethanol, and p.a ethanol, respectively. The three extracts can be grouped based on FTIR spectrum data using principal component analysis using three principal components with a total variance of 93%. The results obtained show that using different extracting solvents provides different antioxidant capacities and phytochemical profiles.
Antioxidant Capacity, Phytochemical Profile, and Clustering of Pomegranate (Punica granatum L.) Peel Extracts Using Different Solvent Extraction Mohamad Rafi; Laela Wulansari; Dewi Anggraini Septaningsih; Tsania Firqin Purnomo; Reza Auliatifani; Khaydanur Khaydanur; Auliya Ilmiawati; Wina Yulianti; Nunuk Kurniati Nengsih; Irma Herawati Suparto; Wisnu Ananta Kusuma
Journal of Tropical Life Science Vol. 11 No. 3 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.03.14

Abstract

Pomegranate has valuable nutritional content and contains various bioactive compounds, one found in the fruit's peel. The utilization of these bioactive compounds could be used as herbal medicines and supplements, such as antioxidants. This study aimed to determine the antioxidant capacity, phytochemical profile, and pomegranate peel extract grouping using different extracting solvents. The three extracting solvents used were water, 70% ethanol, and ethanol p.a. Antioxidant capacity of the three extracts was measured using the DPPH and CUPRAC methods. We also determined the total phenolic and flavonoid levels and the TLC fingerprint analysis and FTIR spectrum of the pomegranate peel extracts. The 70% ethanol extract owned the largest antioxidant capacity than the other two extracts with a value of 358.67 and 2981.59 µmol trolox/g dried sample using the DPPH and CUPRAC methods, respectively. The three pomegranate peel extracts' total phenolic and flavonoid levels ranged from 287.26–1068.81 mg GAE/g dried sample and 0.24-0.75 mg QE/g dried sample. TLC fingerprint analysis of pomegranate peel extract yielded 2, 6, and 6 bands for water extract, 70% ethanol, and p.a ethanol, respectively. The three extracts can be grouped based on FTIR spectrum data using principal component analysis using three principal components with a total variance of 93%. The results obtained show that using different extracting solvents provides different antioxidant capacities and phytochemical profiles.
Co-Authors Abdul Rohman Adiba, Asfiah Agusta, Dhea Demitri Akhiruddin Maddu Alfi Hudatul Karomah Andi Thaariq Muhammad Arinana Arinana Arini Septianti Asep Hidayat Asep Hidayat Asyari, R. Mugni Chairil Arbi Auliatifani, Reza Ayu Rahmania Lestari Bambang Pontjo Priosoeryanto Budi Arifin Budi Arifin Budi Arifin Budiarti, Eka Desi Wulandari, Desi Eka Budiarti Erlina, Iis Fadia Fahira Fadilah, Nisa Lelita Failasufa, Adzkia Febriany, Retno Putri Fiqri, Muhammad Gustini Syahbirin Gustini Syahbirin Hafizah Fatunisa Hanhan Dianhar Ika Resmeiliana Irma Herawati Suparto Irmanida Batubara Ishika Jauza Nasywa Kenang Sagraha, Ghiffari Khaydanur Khaydanur Khaydanur, Khaydanur Kurniawanti, Kurniawanti Laela Wulansari Larasati, Rizki Marsha Lutfia Mutmainnah Luthfan Irfana Luthfan Irfana Luthfan Irfana M. Raffi Rayandhika Melinia Falestin Moch Ikkbal Aditya Kasmaran Mohamad Rafi Mohamad Rafi Mohamad Rafi Muhamad Insanu Mujahidah, Ummahatul Nafisa Muthia Wafa Nanik Siti Aminah Nasution, Lise Meitner Aribah Nazhifah, Athiq Nengsih, Nunuk Kurniati Ningtias, Widya Sekar Ayu Nunuk Kurniati Nengsih Priandanda, Rafi Chandra Pujiyati Pujiyati Pujiyati, Purnomo, Tsania Firqin Purwantinigsih Sugita Purwantiningsih Sugita Purwantiningsih Sugita Rahayu, Dyah Utami Cahyaning Rahmah, Lutfita Anisa Rahmani, Nabiila Reza Auliatifani Risda Fardilya Nihayah Sagraha, Ghiffari Kenang Sandra Arifin Aziz Septaningsih, Dewi Anggraini Setyanto Tri Wahyudi Siti Sadiah Taopik Ridwan Tsania Firqin Purnomo Utami Dyah Syafitri Wina Yulianti Wisnu Ananta Kusuma Wulansari, Laela Yulianti, Dwi Indah