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All Journal Jurnal Kedokteran Brawijaya Majalah Kedokteran Bandung JIMKesmas (Jurnal Ilmiah Mahasiswa Kesehatan Masyarakat) Preventif Journal Pharmaceutical Sciences and Research (PSR)
Lister, I Nyoman Ehrich
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Education Medicine & Pharmacology Public Health

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Journal : Jurnal Kedokteran Brawijaya

 1 
Comparison of Antioxidant and Anti-Tyrosinase Activities of Pineapple (Ananas comosus) Core Extract and Luteolin Compound Vrianty, Dela; Qodariah, Rismawati Laila; Widowati, Wahyu; Sinaga, Ade Putra Fratama; Fibrina, Dewi; Fachrial, Edy; Lister, I Nyoman Ehrich
Jurnal Kedokteran Brawijaya Vol 30, No 4 (2019)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (142.679 KB) | DOI: 10.21776/ub.jkb.2019.030.04.2

Abstract

Free radicals and UV exposure can cause aging. Aging prevention needs substances that can prevent molecular oxidation reactions in cells and inhibit the activity of enzymes that trigger aging. Research on pineapple skin and flesh extract (Ananas comosus (L.) Merr.) reported the presence of luteolin compound which functions as antioxidants and antityrosinase. However, in this study, the object used was pineapple core extract (PCE), which has not been widely known for its antioxidant and antityrosinase activity. Therefore, the purpose of this study was to determine the content of phytochemical compounds, antioxidant activity, and inhibitory activity of tyrosinase enzymes by PCE and then compared with the luteolin (LT) compound test results using Fansworth method, DPPH scavenging activity assay and tyrosinase enzyme inhibition assay. Based on the study results, the phytochemical compounds contained in PCE were tannins and triterpenoids.  PCE and LT produced the highest DPPH scavenging activity, which was 64.86% and 59.32% (final concentration 200μg/ml and 6.25μg/ml) and the highest tyrosinase inhibition activity, which was 60.52% and 85.02% (final concentration 100 μg/ml). Antioxidant activity was determined based on IC50 of 87.46µg/mL and 4.17 µg/ml respectively. IC50 tyrosinase enzyme inhibition EBN and LT respectively at 62.27µg/ml and 5.25 µg/ml. Antioxidant activity through DPPH free radical scavenging test and tyrosinase enzyme inhibition activity by PCE was lower than LT.
Antioxidant and Anti-Hyaluronidase Activities of Dragon Fruit Peel Extract and Kaempferol-3-O-Rutinoside Liana, Liana; Rizal, Rizal; Widowati, Wahyu; Fioni, Fioni; Akbar, Khainir; Fachrial, Edy; Lister, I Nyoman Ehrich
Jurnal Kedokteran Brawijaya Vol 30, No 4 (2019)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (172.286 KB) | DOI: 10.21776/ub.jkb.2019.030.04.3

Abstract

Aging is a natural process in human life and is triggered by the presence of free radicals (ROS). The use of antioxidants from natural ingredients is one of the breakthroughs to overcome aging and counteract the harmful effects caused by the free radicals. This study aimed to determine and compare the antioxidant activity of H2O2 scavenging and hyaluronidase inhibition of red dragon fruit peel extract (DFPE) and kaempferol-3-o-rutinoside (KOR) compounds. Dragon fruit peel extract (DFPE) is obtained through extraction by maceration method using 70% ethanol solvent. The design of this study included antioxidant and anti-aging activity assay of EKBN and KOR at the series concentration of 15.63; 31.25; 62.50; 125; 250; 500 µg/mL through H2O2 scavenging, as well as the DFPE and KOR hyaluronidase inhibition assay at the series concentration of 5.21; 10.42; 20.83; 41.7; 83.33; 166.67 µg/mL. EKBN shows that the average activity of H2O2 scavenging is lower than KOR. In addition, the IC50 values of KOR for H2O2 scavenging is lower (351.46±2.30ug/mL) than DFPE (409.64±23.17ug/mL). While, KOR also has higher values of inhibitory activity than of the DFPE. However, the IC50 value of KOR for hyaluronidase inhibition activity was 84.07±10.46µg/mL, equivalent to the IC50 value of DFPE (85.32±10.24µg/mL). The presence of antioxidant and anti-aging activity in the EKBN is probably caused by betalain and the KOR compound itself contained in red dragon fruit. The results of the paired-samples T-test on antioxidant activity and anti-aging of DFPE and KOR showed non-significant difference. Thus, DFPE has an equivalent antioxidant and anti-aging through H2O2 scavenging and hyaluronidase activity as possessed by the KOR compound.
Co-Authors AA Sudharmawan, AA Ainy, Al-Annisa Fadhila Armansyah, Adek Edy Fachrial Ferdinand, Sahna Fibrina, Dewi Fioni, Fioni Geeta, Ginting, Chrismis Novalinda Girsang, Ermi Hanna Sari Widya Kusuma, Hanna Sari Widya Intisari, Yayo Irwan, Mulia Jusri, Ridza Khainir Akbar, Khainir Liana Liana Marpaung, Hans Hotma Nabila, Pivi Nasution, Ali Napiah Purba, Rosi Krisdayanti Qodariah, Rismawati Laila Rizal Rizal Silaban, Agnes Novica Putri Sinaga, Ade Putra Fratama Siregar, Hafiz Hawari Siregar, Irma Dolsyeria Sofiyana, Maria Sormin, Delores Elisabeth Tarigan, Nuzulul Akbar Vrianty, Dela WAHYU WIDOWATI Widodo, Wahyu Setia