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Isolasi, Karakterisasi dan Amobilisasi α-Amilase dari Aspergillus niger FNCC 6018 Nisa', Khairun
Chem Info Journal Vol 1, No 1 (2013)
Publisher : Chem Info Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (328.352 KB)

Abstract

Aspergillus niger FNCC 6018. The purposes of this research are to get specific activity data of isolated α-amylase from Aspergillus niger FNCC 6018, get optimum condition of enzyme data, include: pH and temperature, and get activity data of immobilized α-amylase in matrix bacto agar and recurrence test. The method is use in this research to determine α-amylase activity test by measuring maltose that produce from α-amylase which hydrolize amylose with Bernfeld method using DNS (3,5-dinitrosalicylic acid). Amount of protein was measured by Lowry method using standard BSA (Bovine Serum Albumin). The result of this research show that α-amylase enzyme isolated from Aspergillus niger FNCC 6018 which ammonium sulphate fraction 3 (40-60%) has the highest specific activity amount 5.7 Unit/mg protein. Optimum condition α-amylase from Aspergillus niger FNCC 6018 was achieved at optimum pH value 5.0 and temperature 36οC. Immobilized α-amylase in matrix bacto agar had specific activity amount 1.95 Unit/mg protein and use until twice recurrence.
HOTS-Oriented Student Worksheets with Blended Learning: Improving Students' Science Process Skills Nurulsari, Novinta; Nisa', Khairun; Viyanti, Viyanti; Yassine, Brahimi
Online Learning In Educational Research (OLER) Vol 3, No 1 (2023): Online Learning in Educational Research
Publisher : CV FOUNDAE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58524/oler.v3i1.228

Abstract

Today's education must meet the needs of 21st-century learning and developments in the digital age. HOTS and science process skills must be trained to carry out scientific investigations. This experimental research aims to identify the improvement of students' science process skills affected by HOTS-oriented student worksheets through blended learning conducted in one of the high schools in Pringsewu District, Lampung Province. The subjects of this study were students of class X SMA Negeri 2 Pringsewu, with a population of 180 people. The research sample was selected using a purposive sampling technique so that class MIPA 2 was obtained as an experimental class with a total of 29 students and MIPA 4 class as a control class with a total of 31 students. The research method used was a non-equivalent control group design which aims to look for the effect of a causal relationship between two factors. The data collection technique used was a pretest and posttest design using a science process skills instrument. The n-gain results show an increase in 5 indicators of students' science process skills, where the increase occurs in the medium category. The results showed an increase after using HOTS-Oriented Student Worksheets (SW) on impulse-momentum material through blended learning to improve students' science process skills.
Negotiating Gender Equality: NU Scholars’ Perceptions of Women’s Role in Sumenep Rozi, Achmad Bahrur; Jamila, Lailatul; Nisa', Khairun; Rosmini, Rosmini; Ulfah, Ulfah
Islamica: Jurnal Studi Keislaman Vol. 20 No. 1 (2025): September
Publisher : Postgraduate Studies of UIN Sunan Ampel Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15642/islamica.2025.20.1.52-75

Abstract

The 1997 National Conference of Nahdlatul Ulama (NU) Scholars in Lombok issued a groundbreaking fatwā permitting women to hold the position of vice president—a progressive step in advanc­ing gender justice within Islamic discourse. However, at the grassroots level, gender segregation remains deeply entrenched, particularly in Sumenep Regency. This study explores how NU feminists in Su­menep negotiate their struggle for gender equality and how local kiai and religious scholars respond to these efforts. Employing a qualita­tive approach with data collected through literature review, observa­tion, interviews, and documentation, this research is framed by Is­lamic feminist theory. The findings reveal that gender discourse in NU Sumenep remains weak when confronted with the religious authority of kiai and traditional scholars. Their ambivalent and skeptical re­sponses toward gender equality activism indicate that the discourse promoted by the NU Central Board (PBNU) has not been effectively internalized at the local level. Furthermore, the absence of pesantren-based initiatives on gender awareness perpetuates gender segregation, resulting in unequal competencies and opportunities between male and female students. This study highlights the gap between NU’s pro­gressive theological discourse and its limited cultural translation among rural communities.
Assessing Secondary Students’ Attitudes Toward Global Warming Putra, Bayu Eka; Suhandi, Andi; Aviyanti, Lina; Amiruddin, Mohd Zaidi Bin; Nisa', Khairun
Jurnal Ilmiah Pendidikan Fisika Vol 9, No 2 (2025): JUNE 2025
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jipf.v9i2.15319

Abstract

Global warming constitutes a critical topic that must be understood by students, given its far-reaching impacts on the environment, human life, and planetary sustainability. Students’ attitudes and responses toward the causes, consequences, and mitigation strategies of global warming play a pivotal role in fostering ecological awareness and promoting environmentally responsible behavior from an early age. This study aims to explore students' attitudes toward global warming in the context of physics education, focusing on their understanding of the issue and its impact on their perspectives on related policies and actions. This study employed a quantitative approach involving 40 tenth-grade students from a senior high school in Indonesia who were studying under the Kurikulum Merdeka. Data were collected using a 4-point Likert scale that measured students' attitudes towards seven main aspects related to global warming. The results showed that the majority of students have a positive attitude towards integrating material on global warming into physics learning and demonstrate awareness of the importance of addressing this issue. However, there are differences of opinion regarding policies on the use of fossil energy and alternative energy. Some students still show a conservative attitude towards fossil energy policies, while others support the policy of using alternative energy that is more environmentally friendly. This study provides important insights into the importance of integrating environmental issues in physics education and its role in shaping a younger generation that is more aware of climate change. The findings are expected to contribute to the development of educational programs that are more relevant and meaningful in raising environmental awareness and sustainability among students.