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All Journal e-Journal Pustaka Kesehatan Journal of Mathematical and Fundamental Sciences STOMATOGNATIC- Jurnal Kedokteran Gigi UNEJ e-Proceeding Dental Journal (Majalah Kedokteran Gigi) BIOEDUKASI Jurnal Material Kedokteran Gigi
Pujiana Endah
Bagian Biomedik, Fakultas Kedokteran Gigi, Universitas Jember
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Earth & Planetary Sciences Education Environmental Science Health Professions Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics

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INFEKSI JAMUR CANDIDA PADA PENDERITA HIV/AIDS Lestari, Pujiana Endah
STOMATOGNATIC- Jurnal Kedokteran Gigi Vol 10, No 1 (2013)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

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Abstract

HIV / AIDS remains a global problem, more than 34 million people infected with HIV / AIDS. When a person with HIV / AIDS will become vulnerable to opportunistic infections. Manifestations of Candida fungal opportunistic infections in people with HIV / AIDS is most commonly a Oropharyngeal candidiasis. In addition, oral fungal infections include: acute pseudomembranous candidiasis, acute atrophic candidiasis, chronic atrophic candidiasis, chronic hyperplastic candidiasis and candida cheilosis. Fungal infections are common in people with HIV / AIDS as it relates to the level of immunosuppression, which can be an indicator of HIV infection and prediction of progression to AIDS infection.
PERAN FAKTOR VIRULENSI PADA PATOGENESIS INFEKSI Candida albicans Lestari, Pujiana Endah
STOMATOGNATIC- Jurnal Kedokteran Gigi Vol 7, No 2 (2010)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

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Abstract

Candida albicans infection is a fungal infection that most often encountered in the clinic and often recurrent. C. albicans infection remains a significant clinical problem. C. albicans can cause severe infection in mucosal and systemic, especially in patients with immunological disorders. Pathogenesis of C. albicans infection through the development of the virulence factors and specific strategies for success in colonization, infection of thehost tissue. The purpose of this paper is to describe the role of virulence factors in pathogenesis of C.albicans infection. In C. albicans infection, the role of virulence factors are very important, in addition to the role of an impaired immune status. Some virulence factors involved in infection of C. albicans are phenotypic switching, dimorfims morphology, adhesion, secretion of hydrolytic enzymes and others.
Perlekatan Streptococcus mutans pada Aplikasi Fissure Sealant Berbahan Resin Dibandingkan dengan Ionomer Kaca Fuji VII (The Attachment of Streptococcus mutans for Fissure Sealant application Made of Resin Compare With Glass Ionomer Fuji VII) Nazala Zetta Zettira; Niken Probosari; Pujiana Endah Lestari
Pustaka Kesehatan Vol 5 No 3 (2017)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Background : Dental caries is the hard tissue disease. Caries is caused by multiple factors that is of them are morphology the surface of the tooth and bacteria Streptococcus mutans. The pit and fissure are primary habitats of S. mutans. Therefore, prevention of fissure sealants are needed. The materials of fissure sealant are composite resin and glass ionomer fuji VII. S. mutans can attach to foreign objects, one of them is material restoration. Objective : The purpose of this research is to know the difference of S. mutans attachment for fissure sealant application made of resin and glass ionomer fuji VII. Method : The experimental laboratory was post test only control design. The samples size from two group are 32 samples. The tool that used is spectofotometer with unit of measurement is colony forming unit a mL. Result and Conclusion : The average of S. mutans attachtment of composite resin and glass ionomer are 5,201 x 108 and 3,963 x 108. The attachtment was tested by independent sample T-tes there was differences of S. mutans attachment for fissure sealant application made of resin and glass ionomer fuji VII. Resin composite have a higher total number than ionomer fuji VII. Keywords: dental caries, fissure sealant, glass ionomer, resin, s. mutans,
Pengaruh Pemberian Monosodium Glutamat pada Induk terhadap Fase Erupsi Gigi Anak Tikus Wistar (Rattus norvegicus) Generasi Pertama (The Effect of Monosodium Glutamate Administration on Mothers Rat (Rattus norvegicus) to the Tooth Eruption Phase of the Fir Mindiya .; Didin Erma Indahyani; Pujiana Endahl Lestari
Pustaka Kesehatan Vol 5 No 3 (2017)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Background: Monosodium glutamat (MSG) is a kind of food additives which consumes widely in society. It may damage the hypothalamic arcuate nucleus and disturb the endocrin regulation system. This condition may delay the tooth eruption phase. Aim: To analyze the effect of MSG administration on the neonatal rats tooth eruption phase, whose mothers were given MSG during gestational period also during gestational and lactation period, to determine the difference between neonatal male and female tooth eruption phase. Materials and Methods: The first molar of right mandibular of neonatal rats from the control group (K1), the treatment group which mothers were given MSG during pregnant period (K2) and the mothers which were given MSG during pregnant and lactation period (K3) were collected as samples. The samples were examined by periapical rontgent to observe the tooth eruption phase. The result was measured by Corel Draw X3. Results and Conclusion: The tooth eruption phase in the treatment group (K2 and K3) are slower than the control group (K1), and there is no difference between neonatal male and female tooth eruption phase. Kata Kunci: hypothalamic arcuate nucleus, monosodium glutamate, tooth eruption
Hubungan Merokok Bernikotin terhadap Penurunan Volume Saliva pada Perokok di Kabupaten Tulungagung (Relationship of Smoke Containing Nicotine to Decrease in the Volume of Saliva in Smokers at Tulungagung District) Ichdanisa Fitriasani; Pujiana Endah Lestari; Leni Rokhma Wati
Pustaka Kesehatan Vol 5 No 3 (2017)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Back Ground: Nicotine is one of the contents of cigarette that bad for health. Nicotine has an influence on the nervous system, which one led to decrease in saliva production. Object: Tulungagung is a district where has a high number of smokers, also there is an identical smoking habit called nyethe. The aim of this study was to determine relationship of the effect of smoking that contains nicotine to decreased in saliva production. Method: The research method used an analitic obsevational study with cross sectional observation. The study was conducted by taking saliva samples of 100 smokers and 100 non smokers at district Tulungagung. Result: The pearson correlation test result showed that the significancy value (sig (2-tailed)) was 0.00 which was smaller than α (0.05) and had a value of peason correlation 0f -0,566. Conclusion: The test result obtained from the pearson correlation was able to explained that nicotine had a relation with saliva volume decreased. The result showed that smoke containing nicotine had influence on reducing the volume of saliva, more often smoke containing nicotine so the lower volume of saliva produced. Keywords: cigarette, nicotine, saliva, tulungagung
ROBUSTA COFFEE BEANS DECREASE OF INFLAMMATION IN DENTAL CARIES I Dewa Ayu Ratna Dewanti; I Dewa Ayu Susilawati; Pujiana Endah; Roedy Budirahardjo
UNEJ e-Proceeding Proceeding of 1st International Conference on Medicine and Health Sciences (ICMHS)
Publisher : UPT Penerbitan Universitas Jember

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Abstract

The coffee plant is one commodity that is developedin kabuapaten Jember. Coffee chemical constituents,such as flavonoids, xanthine, antioxidants, alkaloids,polyphenols may serve as anti-inflammatory,antibacterial, platelet aggregation. Polyphenolbioavailability of coffee has also been studied.1.2.3While Namboodiripad, K. Srividya (2009) provedtheir coffee inhibition zone against S. mutans. Theimmune response of dental caries among othersrelated to cytokines are expressed odontoblast layer,such as IL-1β, IL-1α and TNF-α.4.5 Thus it is said thatcoffee is thought to inhibit dental caries by means ofmodulating the immune response. This niaccordance with the Decree of the Minister of Statefor Research and Technology of the Republic ofIndonesia Number: 02 / M / Kp / II / 2000 onDevelopment and Engineering for NationalDevelopment (JAKSTRA 2000-2004) in the field ofdentistry which has two areas of research focusinclude health field for prevention and treatment ofdiseases and new materials related to ceramics,polymer composites, engineering materials(materials science and engineering), as well as newmaterial derived from ingredients that are natural.6One of the natural ingredients that are known toeveryone is coffee. Where coffee is one of theexcellent programs that can improve theperformance of Jember University's Strategic Plan.This type of coffee is widely known in Indonesiathere are three kinds, namely Arabica, Robusta andLiberika. In general, farmers grow robusta, becausein this type of coffee has more superior properties,so it is very fast growing. Even the coffee is thedominant species of coffee plantations in Indonesia.In addition, it contains prolifenol Robusta coffee,caffeine and chlorogenic acid, which is higher thanthe other coffee types. Currently, the production ofrobusta coffee production reached third copiesworldwide.7.8 Coffee brews are very widelyconsumed in the world due to their pleasant flavorand taste as well as for their stimulating effect onthe mental and physical activities. Several years ago,in California, a research conducted by TakayukiShibamoto, a professor of environmental toxicology,found that freshly brewed coffee contains potentantioxidants equal to the amount found in threeoranges. Antioxidants in general have been linked toa number of potential health benefits, includingprotection against heart disease and cancer,protection against liver and colon cancer, type 2diabetes, and Parkinson's disease..9 Coffee chemicalconstituents, such as flavonoids, xanthine,antioxidants, alkaloids, polyphenols may serve asanti-inflammatory, antibacterial, plateletaggregation.1.2.3 Roasted coffee also has antiadhesiveproperties which prevent adhesion of S.mutans and other harmful materials on the teethand whiten teeth.4 Previous research by a team ofother researchers of Excellence coffee beans for thetreatment of cancer of the oral cavity, an article onPotential immunomodulatory coffee beans to dentalcaries and the potential immunomodulatory Robustabeans to dental caries.10.11.12 In this study Robustabeans made in the form of pasta because pastaShape is the most popular because it can be easilyused and harden quickly.Capping pupa is one of the more conservativetreatments to prevent dental caries has been on thepulp or pulp exposure due to mechanical factors.Interest Capping pulp to maintain the vitality ofdental pulp. Pulp tissue is important in the formationof secondary dentine and reparative dentin inresponse to biological and pathological stimuli.Capping pulp is the application layer or theprotective material or materials for treatment overan open pulp, such as calcium hydroxide which willstimulate the formation of reparative dentin.13Capping pulp after the treatment, the healingprocess will occur in the pulp tissue covering theinflammatory response, collagen formation and theformation of new dentin. Inflammatory responseshould occur as a sign of the host defense. Acuteinflammation occurs a few minutes, hours or days,while chronic inflammation occurs after acuteinflammation. Inflammatory played by inflammatorycells such as PMN (polymorphonuclear,mononuclear cells (monocytes, macrophages,lymphocytes, mast cells, plasma cells). PMN are cellsleading that respond to injury and most frequentlyProceeding ICMHS 2016 ISBN 978-602-60569-3-1174seen in inflammation. These cells react in the earlystages of inflammation (acute) up to 28 hours,followed by macrophages, lymphocytes and plasmacells (chronic).14 Chronic inflammation is said to bethe beginning of the healing process. Although theresponse innate was important in inflammation, butan adaptive response also plays a role, becausecytokines were producted by T cells induceinflammation. To obtain optimal care, the necessaryunderstanding in depth about how the composition,antibacterial properties, biocompatibility, effect ondentin and pulp as well as the technique of using aresin adhesive as a material Capping pulp directly. Inhandling damage teeth, either by decay or trauma,maintain vital pulp tissue remains the mostimportant things to be done by a dentist because ofthe non-vital teeth, the teeth tend to be susceptibleto fracture. The development of the field ofconservation science teeth adapted to thedevelopment of basic science related andtechnological advances applied which is thedevelopment of science in the field of clinical(clinical science). Dental conservation therapy aimsto maintain its position of teeth as long as possible inorder to work longer. This objective can be achievedby treating the hard tissue or soft tissue of a tooth totooth structure back to normal, or at least close tonormal.15
Analysis of protein profile of neem leaves juice (azadirachta indica l. Juss) I Dewa Ayu Ratna Dewanti; I Dewa Ayu Susilawati; Pujiana Endah Lestari; Roedy Budirahardjo
UNEJ e-Proceeding 2016: Proceeding The 1st International Basic Science Conference
Publisher : UPT Penerbitan Universitas Jember

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Abstract

The current trend of the management of diseases has shifted toward modulating the immune response of the patients. This is achieved by the administration of immunomodulatory substance obtained from the immunogenic medicinal plant, such as neem. The immunogenic properties of neem has been associated with its protein content. Aim. This study aimed to characterize the proteins and immunogenic components of neem leaves juice using sodium dodecyl Sulphate method Polyacrilamide gel electrophoresis (SDS-PAGE). Materials and Methods. Neem leaf juice was extracted for proteins with freeze dry method. Samples that were to be run were added with RSB with the ratio of 1:1, then heated in boiled water for ± 5 minutes. An amount of 15 µl from each sample was then put into the wells. Samples were run in 120 Volt for 60-80 minutes. Gel was then taken, stained with silver nitrate for 1 hour, and was then stained every 1.5 hours. Protein bands formed were then observed. Results. The protein fraction of neem leaves juice consisted of proteins with molecular weights (MW) as follows: 11 kDa, 13 kDa, 30 kDa, 62 kDa, 70 kDa, 81 kDa. Conclusion. All protein fractions of neem leaves juice are potentially immunogenic components.
ROBUSTA COFFEE BEANS INCREASE LEVELS OF TNF-α AS A RESPONSE TO Streptococcus mutans Pujiana Endah Lestari; I Dewa Ayu Susilawati; I Dewa Ayu Ratna Dewanti; Roedy Budirahardjo
UNEJ e-Proceeding Proceeding of 1st International Conference on Medicine and Health Sciences (ICMHS)
Publisher : UPT Penerbitan Universitas Jember

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Abstract

Coffee plant is commodity exports that wasencouraging because it has relatively high economicvalue in the world market. Coffee plantations aremanaged by people, and this day its grow continuallyin several provinces in Indonesia so expansion isincreasing continuously. Coffee production inIndonesia ranks the fourth largest in the world afterColombia, Brazil and Vietnam (Zainuddin andMurtisari, 1995; Simanihuruk and Sirait, 2010). Inaddition, the coffee plant is one of the leadingcommodities developed in Jember. Chemical contentof coffee such as flavonoids, xanthine, antioxidants,alkaloids, polyphenols can serve as a bitter taste,antibacterial, platelet aggregation. Thebioavailability of polyphenols coffee has also beenstudied (Scalbert et al., 2000; Coralie et al., 2006;Naziq, 2012).Namboodiripad and Srividya (2009) prove theexistence of the inhibition zone coffee against S.mutans. These bacteria are structurally andantigenetically express a surface protein that arecalled antigen I / II, B, Sr and PAC that have amolecular weight of 185 kDa. These antigens by theresearchers were assigned that play a role in thepathogenesis of dental caries, and its are effective asa vaccine in the prevention of dental caries. I/IIantigens of S. mutans have properties of adhesive,that the bacteria are used attachment to hostcomponents during colonize and infection, so itsbecome the focus of a number of researchers. Thesesurface-antigen proteins have an effect in theattachment of S. mutans with acquired pellicles ontooth surfaces (Yuliati, 2005). The immune responsesof dental caries were associated with cytokine suchas IL-1 β, α, IL-1 and TNF-α that were expressedodontoblast layer. It is said that bacteria excretion aextracellular virulence-immunomodulatory protein(VIP, which has a mitogenic effect on lymphocytes,suppress the immune response of the host andinduces production of IL-10, which is animmunosuppressor cytokines. So, VIP is animportant virulence factor produced microorganismsand closely associated with the pathogenicity ofbacteria (Gomes et al., 2006).Thereby, it is said that coffee could be expectedto inhibit dental caries by means of modulate theimmune response. The paradigm change stated thatthe tissue replacement turns into tissueregeneration that was cause approach of medicalmateria that geared to a biologically tissueimprovement. Biocompatible materials indicate thatit can be accepted by the body (Harty and Ogston(1995), and the coffee was clearly meets theserequirements.The current utilization of the coffee plant in themodulates an immune response against S. mutansthat is cause a dental caries that have not beenstudied, so arises problem how theimmunomodulatory potential of robusta coffeebeans against dental caries ?The general objective of this research is to analyzethe immunomodulatory potential of robusta coffeebeans against dental caries, whereas the aim inparticular is analyze the influence of robusta coffeebeans against TNF-α levels in response to S. mutans.
The role of Kuniran (U. moluccensis) and Gurami (O. goramy) fish thorns and scales in increasing salivary leukocyte and monocyte cells viability against Streptococcus mutans I Dewa Ayu Ratna Dewanti; I Dewa Ayu Susilawati; P. Purwanto; Pujiana Endah Lestari; Roedy Budirahardjo; Dyah Setyorini; Ristya Widi Endah Yani; Erawati Wulandari; Melok Aris Wahyukundari
Dental Journal (Majalah Kedokteran Gigi) Vol. 52 No. 1 (2019): March 2019
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

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Abstract

Background: Kuniran thorns and Gurami fish scales are rich in protein and minerals such as dentin believed to increase cell viability against Streptococcus mutans (S. mutans) that causes dental caries. These, in turn, can cause systemic diseases if left untreated. Purpose: This study aims to analyze the influence of Kuniran thorns and Gurami fishes scales on the viability of monocytes and salivary leukocytes against S. mutans. Methods: Monocytes and leukocytes salivary cells were placed on a microtiter plate and treated according to the nature of each group. This study comprised the following groups: control group: untreated; S. mutans group: induced by S. Mutans; Gurami thorn group: thorns + S. Mutans; Gurami scales group: scales + S. Mutans; Kuniran thorn group: thorns + S. Mutans; Kuniran scales group: scales + S. mutans. Viability analysis involved staining with Tripan Blue. Furthermore, the number of viable cells (white) was calculated under an inverted microscope at 200 times magnification from five fields of view. Data was analyzed by means of an ANOVA test followed by LSD test. Results: The ANOVA and LSD tests confirmed significant differences (0.01<P). Kuniran thorns and Gurami fish scales increased the viability of monocytes and salivary leukocytes, but not significantly. The content of flavonoids, amino acids, omega 3, omega 6 and antioxidants increased leukocyte metabolism, thereby increasing cell resistance to S. mutans infection. Conclusion: Kuniran thorns (U. moluccensis) and Gurami (O. goramy) fish scales increase the viability of salivary leukocyte and monocyte cells against Streptococcus mutans.
Robusta Coffee (Coffeacanephora) Decreasing IL-1α (Interleukin-1α) Expression and Increasing the Number of Fibroblasts in Healing Process in Dental Pulp in Wistar Rats I Dewa Ayu Ratna Dewanti; I Dewa Ayu Susilawati; Pujiana Endah Lestari; Ristya Widi Endah Lestari; Erawati Wulandari; Roedy Budirahardjo; Dyah Setyorini; Sunlip Wibisono
Journal of Mathematical and Fundamental Sciences Vol. 51 No. 1 (2019)
Publisher : Institute for Research and Community Services (LPPM) ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.math.fund.sci.2019.51.1.6

Abstract

Fibroblasts and IL-1α are important components of inflammation and healing in dental caries. This healing allegedly could be enhanced by robusta coffee. The aim of this study was to investigate the effect of varying concentrations of robusta coffee on IL-1α expression and the number of fibroblasts. Wistar rats were used, divided into 4 groups and 3 subgroups (7-, 14"‘, and 21-day rats) with each group containing 4 animals: Control group: untreated group; 25% Coffee Group: cavities + treated with 25% coffee bean paste; 50% Coffee Group: cavities + treated with 50% coffee bean paste; 75% Coffee Group: cavities + treated with 75% coffee bean paste. The 7-, 14-, and 21-day rats were sacrificed serially for analysis of the number of dental pulp fibroblasts by staining with HE and IL-1α expression by immunohistochemistry. The resulting data were analyzed descriptively and using ANOVA, followed by an LSD test. The higher the concentration of robusta coffee, the more the expression of IL-1α decreased and the more the number of fibroblasts increased. It is suspected that immunomodulatory proteins inhibit IL-1α expression. It was concluded that robusta coffee could decrease IL-1α expression and increase the number of fibroblasts during the healing process in the dental pulp of Wistar rats.
Co-Authors Atik Kurniawati, Atik Didin Erma Indahyani Dyah Setyorini Erawati Wulandari Erna Sulistyani Happy Harmono I Dewa Ayu Ratna Dewanti I Dewa Ayu Susilawati Ichdanisa Fitriasani Iin Eliana Triwahyuni Leni Rokhma Wati Lutfi Lailia Sofidiana Mahardiani Dwi Astanti Maheswari, Gardenisti Putri Masari, Al Melok Aris Wahyukundari Mindiya . Morauli, Fatricia Nailufar, Khanun Nazala Zetta Zettira Niken Probosari Nindita Cahya Mumpuni P. Purwanto Peni Pujiastuti Prihanti, Ayu Mahartini Raditya Nugroho Ristya Widi Endah Lestari Ristya Widi Endah Yani Roedy Budirahardjo Salsabilla Milatul Mirza Sri Hernawati Sunlip Wibisono Syahrani, Nafila Yumnaina Nurhadi Zahreni Hamzah