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Analisis Kebocoran Tepi pada Glass Ionomer Kaca dengan Penambahan Bioactive Glass Berbasis Silica dari Ampas Tebu Catur Putri Kinasih; Didin Erma Indahyani; Izzata Barid; Niken Probosari
STOMATOGNATIC - Jurnal Kedokteran Gigi Vol 15 No 2 (2018)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/stoma.v15i2.17931

Abstract

The use of Glass Ionomer Cement (GIC) has some limitation, such as water sensitivity which leads to formation of microleakage due to shrinkage and brittle. Incorporation of 0.04 wt% bioactive glass nano silica (BAG) with GIC enhances its bioactivity to forming hydroxycarbonate apatite (HCA) leading to preventing of marginal gap formation. The objective this study was To determine the difference of microleakage means value between GIC and GIC which has been added by BAG from bagasse. In this study making BAG from bagasse, than choose the samples (24 bovine) randomly and grouping them to be 4 groups, which are group 1, GIC; group 2, GIC+Vaseline, group 3, GIC+BAG; group 4 GIC+BAG+Vaseline. All of the samples are supposed to be preparation, placed of the restoration, and stored in the aquadest then methylen blue 1% in sequence at 37oC until 24 h. The microleakage means value is decided by scoring system depend on the penetration of methylen blue 1% at occlusal wall and gingival wall. The microleakage means value on GIC+BAG is smaller than GIC, but their differences were not significant.
Aktivitas Antioksidan dan Total Polisakarida Ekstrak Rumput Laut Merah, Hijau dan Coklat dari Pantai Jangkar Situbondo Didin Erma Indahyani; Depi Praharani; Izzata Barid; Ari Tri Wanodyo Handayani
STOMATOGNATIC - Jurnal Kedokteran Gigi Vol 16 No 2 (2019)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/stoma.v16i2.23094

Abstract

The antioxidant activity and polysaccharide composition of seaweed is influenced by species (red, green and brown seaweed), geographical conditions, extraction methods, solvents, temperature and the length of time of extraction. The purpose of this study was to known the total polysaccharide and antioxidant activity in red, green and brown seaweed extracts from Jangkar Situbondo beach. Method. The study was conducted on red, green and brown seaweed extracted with ethanol and water to determine total polysaccharides. Antioxidant analysis uses the method 2,2-diphenyl1-picrylhydrazyl (DPPH) radical. Total polysaccharide and antioxidant activity were observed statistically using two-way ANOVA. The results of this study show that the polysaccharide content in seaweed water extract was significantly higher than ethanol extract, especially in brown seaweed. Seaweed water extracts showed higher antioxidant activity than ethanol extracts. The antioxidant activity of brown seaweed was significantly higher than that of green and red seaweed. Conclusion. Seaweeds have high antioxidant activity in water extracts. Brown seaweed water extract contains high polysaccharides than green and red seaweed. Antioxidant activity in brown seaweed is also higher than in green and red seaweed.
Kelarutan kalsiumemail pada saliva penderita tuna netra (Dissolution of enamel calcium in saliva of patients with visual impairment) Hendri Jaya Permana; Didin Erma Indahyani; Yenny Yustisia
Journal of Dentomaxillofacial Science Vol. 13 No. 3 (2014): Formerly Jurnal Dentofasial ISSN 1412-8926
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v13i3.406

Abstract

Patients with visual impairment experienced debris, calculus and a high index of oral hygiene. Low salivary flow anddifficulty in cleaning the oral cavity in patients with visual impairment caused high accumulation of plaque that causesa decrease in the pH of saliva. Low pH of saliva affects the balance of the composition of inorganic email. Calcium isthe largest inorganic content of the email, thus dissolving calcium email can be an indication of enameldemineralization. This study aimed to determine the solubility of calcium in saliva of blind patients, and salivary pHrelationship with the solubility of calcium. This experimental laboratory study used saliva taken from 8 blind patientsand artificial saliva as a medium of enamel pieces soaking for 24 hours. Dissolved calcium levels were measured byatomic absorption spectrophotometer. The results showed that the solubility of calcium in the saliva of blind patientsgreater than the control group (p<0.05). Pearson correlation test demonstrate the relationship between salivary pHand solubility of calcium enamel, so the lower the pH the greater enamel calcium dissolution. It was concluded that themagnitude of the solubility of calcium enamel due to decreased of salivary pH.
Studi pendahuluan silika amorf sekam padi sebagai scaffold sintetis bone graft: efeknya terhadap aktivitas sel osteoblas (In-vitro) Didin Erma Indahyani; Zahreni Hamzah; Pujiana Endah Lestari
Jurnal Material Kedokteran Gigi Vol 1 No 1 (2012): JMKG Vol 1 No 1 Maret 2012
Publisher : Ikatan Peminat Ilmu Material dan Alat Kedokteran Gigi (IPAMAGI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (286.742 KB)

Abstract

Synthetic bone graft material was developed as a scaffold that serves to template the formation of bone. The ideal scaffold should be biodegradable, osteoindukctive, and osteoconductive. Silica proven to support and promote bone growth. Rice husk contains silica is quite high. The purpose of this study is to analyze the amorphous silica from rice husk as synthetic bone graft material (scaffold), particularly on osteoblast activity in vitro. The research was conducted by isolating silica from rice husk.The results of isolation would be conditioned on the culture medium of primary osteoblasts. Primary osteoblast cultures derived from rat calvaria wistar age of 2 days, growing in Alpha-Modified Eagle Medium (α-MEM) (as a negative control), α-MEM in conditioning with silica 58s (as a positive control) and α-MEM that conditioned with silica from rice husk (as treatment group). Osteoblast activity was observed by analyzing the expression of alkaline phosphatase, using Alkaline phosphatase Kits at days 7 and 14. The results obtained that the rice husk silica were significantly (p <0.05) has the expression of alkaline phosphatase higher than the control group and potitif group. This may imply that the amorphous silica of rice husk affects the increase in osteoblast activity in vitro. It was concluded that rice husk silica increases osteoblast cell activity that is potentially as scaffold for bone graft synthetic material.
Viskositas dan Porositas Bahan Cetak Alginat dari Alga Merah Kappaphycus alvarezii Rosi Endah Pratiwi; Izzata Barid; Didin Erma Indahyani
STOMATOGNATIC - Jurnal Kedokteran Gigi Vol 19 No 2 (2022)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/stoma.v19i2.34743

Abstract

Alginate impression material is a material that is often used in dentistry to produce study models. The main active ingredient in the alginate impression material is sodium alginate, which can be obtained naturally from algae. Alginate from turbulent water (Waters on village Agel, Situbondo) and the structure of aliginate gluronic acid can form gel and influence the viscosity and the porosity of alginate impression material. Purpose was to analyze the viscosity and porosity of the alginate impression material from the red algae Kappaphycus alvarezii. This study is a laboratory experimental study with the research design of the post-test only control group design. This study consisted of 4 groups, namely the (1) viscosity control group, (2) porosity control, (3) viscosity treatment, and (4) porosity treatment. The porosity measurement results showed that there was no significant difference and the viscosity was significantly different from the control group because of the type of algae, the surface area of the particles, the preparation stage, the extraction that affected sodium alginate. Kappaphycus alvarezii type red alginate impression material has lower porosity than the standard Hygedent alginate impression material with a mean yield of 9%. The viscosity of the Kappaphycus alvarezii red alginate impression material based on the sigma standard is classified as high viscosity with an average of 33,200 cP.
Analisis Ukuran Lengkung Rahang Anak Usia 10-13 Tahun dan 14-16 Tahun di Wilayah Dataran Tinggi (Studi di Kecamatan Jelbuk Kabupaten Jember) Izzata Barid; Didin E. Indahyani; Sigiq E. Utami
e-GiGi Vol. 11 No. 1 (2023): e-GiGi
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/eg.v11i1.44391

Abstract

Abstract: The growth and development of the arch is influenced by genetics, race, and environment. Genetic factors are the important basic in growth and development while environmental factors in the form of geographical, socio-cultural and economic locations can interact with the genetic factors. This study aimed to analyze the size of the jaw arch of children aged 10-13 years and 14-16 years at the highland of Jelbuk District, Jember Regency. This was an analytical and observational study with a cross sectional design. Measurement of the jaw arch on the model was performed using the Raberin method. The results obtained 136 subjects consisting of 59 boys and 77 girls aged 10-16 years. All subjects were grouped into two age groups, 10-13 years and 14-16 years. The Mann-Whitney U test on the difference in jaw arch size between the ages of 10-13 years and 14-16 years showed that most of them had p-values of >0.05, which meant that there was no significant difference between the two age groups, except at L31 of mandibular male. In conclusion, there is no significant difference in the size of the jaw arch of children aged 10-13 years and 14-16 years at the highland of Jelbuk District, Jember Regency Keywords: highlands; the size of the jaw arch; growth spurts; socio-cultural; economy Abstrak: Pertumbuhan dan perkembangan lengkung rahang dipengaruhi oleh genetik, ras, dan lingkungan. Faktor genetik merupakan modal dasar dalam tumbuh kembang sedangkan faktor lingkungan berupa letak geografi, sosial budaya dan ekonomi dapat berinteraksi dengan faktor genetik. Penelitian ini bertujuan menganalisis ukuran lengkung rahang anak usia 10-13 tahun dan usia 14-16 tahun di wilayah dataran tinggi Kecamatan Jelbuk, Kabupaten Jember. Jenis penelitian ini observasional analitik dengan desain potong lintang. Pengukuran lengkung rahang pada model menggunakan metode Raberin. Hasil penelitian mendapatkan 136 subjek yang terdiri dari 59 anak laki-laki dan 77 anak perempuan berusia 10-16 tahun. Keseluruhan subjek dikelompokkan menjadi dua kelompok usia yakni usia 10-13 tahun dan usia 14-16 tahun. Berdasarkan Mann-Whitney U Test pada uji beda ukuran lengkung rahang antara usia 10-13 tahun dengan 14-16 tahun sebagian besar menunjukkan nilai p>0,05 yang artinya tidak terdapat perbedaan bermakna antara kedua kelompok usia, kecuali pada L31 rahang bawah anak laki-laki. Simpulan penelitian ini ialah tidak terdapat perbedaan bermakna dalam ukuran lengkung rahang antara kelompok usia 10-13 tahun dan 14-16 tahun di wilayah dataran tinggi Kecamatan Jelbuk, Kabupaten Jember. Kata kunci: dataran tinggi; ukuran lengkung rahang; growth spurt; sosial budaya; ekonomi
Aktivitas Antibakteri Bahan Cetak Berbasis Ekstrak Natrium Alginat dari Alga Merah (Kappaphycus alverezii) terhadap Lactobacillus acidophilus Putu Sadaka Putri Rahayu; Depi Praharani; Niken Probosari; Didin Erma Indahyani; Izzata Barid
STOMATOGNATIC - Jurnal Kedokteran Gigi Vol 20 No 1 (2023)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/stoma.v20i1.38592

Abstract

Alginate impression material is a material that is often used for impression tissue in the oral cavity, and can be one of the agents of infection transmission in dental practice. Microorganisms can adhere to the mold such as Lactobacillus acidophilus bacteria. L. acidophilus were isolated from active caries and were considered as secondary invaders in the dental caries invasion process. The development of impression materials that have antibacterial capabilities needs to be done, one of which is by using materials derived from red algae (Kappaphycus alverezii). This plant contains a lot of alginate which is the main components of the cell wall, consisting of calcium, magnesium, sodium and potassium alginate salts. Synthesizing sodium alginate from red algae and analyzing the antibacterial activity of sodium alginate-based impression materials from red algae against L. acidophilus. Extraction of alginate from red algae using the acid method and conversion of alginic acid to sodium alginate by adding NaOH. The impression material is made with the addition of sodium alginate. Antibacterial activity test by well diffusion method. This study consisted of 3 groups, namely control (K), red algae sodium alginate extract (P1), and impression material with red algae sodium alginate extract (P2). Groups K, P1 and P2 had an average inhibition zone of 0 mm, 15.56 mm, 17.22 mm, respectively. Kruskal Wallis and Mann Whitney test showed significant differences between groups. Impression material based on sodium alginate extract from red algae has strong antibacterial activity against L. acidophilus.
Stabilitas Dimensi dan Setting Time Bahan Cetak Anatomis Kedokteran Gigi Dari Ekstrak Natrium Alginat Rumput Laut Merah (Kappaphycus alvarezii) Dari Desa Agel, Kecamatan Jangkar, Situbondo Didin Erma Indahyani; Izzata Barid; Agus Sumono; Fitria Arifka Rahman
STOMATOGNATIC - Jurnal Kedokteran Gigi Vol 20 No 1 (2023)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/stoma.v20i1.38597

Abstract

Alginate impression material is important in dentistry. In its clinical application, setting time is still considered too fast, and the alginate impression material must have dimensional stability in accordance with ANSI / ADA standard specification no.18. Alginate impression material has a main component, namely sodium alginate which is produced from the seaweed extraction process. In Indonesia, there are several types of seaweed, one of which is the Kappaphycus alvarezii (K. alvarezii). The seaweed from the village of Agel Situbondo has the potential to be a source of sodium alginate and has antibacterial and antioxidant effects. The aim of this study to analyze the dimensional stability and setting time of the alginate printing material with sodium alginate extract of kappaphycus alvarezii seaweed. The study was conducted using laboratory experimental methods with post-test only control group design, there were 2 groups, namely the commercial alginate printing material control group, and the alginate printing material treatment group with sodium alginate extract from the acid method, then the FTIR test was carried out. Then the two groups were tested for setting time and dimensional stability. The results of the FTIR test of red seaweed extract showed an absorption peak that was identical to that of sodium alginate. The results of the setting time test significantly took longer for the red seaweed alginate impression material than the control group, while the dimensional stability showed the same results. Conclusion: The results of the setting time test showed that the treatment group had a longer setting time than the control group and both groups had dimensional stability according to the standard. The results of the setting time test showed that the treatment group had a longer setting time than the control group and both groups already had dimensional stability in accordance with the standard.
POTENSI BAHAN CETAK EKSTRAK NATRIUM ALGINAT RUMPUT LAUT MERAH (Kappaphycus alvarezii) DALAM MENGHAMBAT PERTUMBUHAN Streptococcus mutans Inneke Dwi Lestari; Depi Praharani; Achmad Gunadi; Izzata Barid; Didin Erma Indahyani; Niken Probosari
B-Dent: Jurnal Kedokteran Gigi Universitas Baiturrahmah Vol 10, No 1 (2023): Vol 10 No 1, Juni 2023
Publisher : Universitas Baiturrahmah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33854/jbd.v10i1.1120

Abstract

Introduction: Synthetic alginate is an impression material that is widely used in dentistry. Dental impressions may carry Streptococcus mutans bacteria, which is cariogenic bacteria. Red seaweed has the potential as a source of sodium alginate and has antibacterial activity. Aim: The purpose of this study is to analyze the ability of impression materials containing sodium alginate from red seaweed in inhibiting the growth of S. mutans bacteria. Methods: This study uses a laboratory experimental method with a post-test-only control group design. The number of research samples is 24, which are divided into group A (control), group B (red seaweed sodium alginate extract), and group C (red seaweed sodium alginate extract impression material). Sodium alginate was extracted from red seaweed (Kappaphycus alvarezii) by the acid method. Inhibition test of the growth of S. mutans bacteria using well diffusion method. Growth inhibition is characterized by the formation of an inhibition zone. Result: The test results showed that group A (control) does not have the ability to inhibit the growth of S. mutans bacteria. Meanwhile, group B (RLM Na- alginate extract) and group C (RLM Na-alginate extract impression material) have the same ability to inhibit the growth of S. mutans bacteria. Conclusions: Red seaweed sodium alginate extract impression material can inhibit the growth of S. mutans
Pembentukan Hidroksikarbonat Apatit Oleh Pasta Injeksi Bioactive Glass Berbasis Silika Abu Ampas Tebu Dan Penambahan Karageenan Rumput Laut Didin Erma Indahyani; Izzata Barid; Riris Aria Dewanti
STOMATOGNATIC - Jurnal Kedokteran Gigi Vol 20 No 2 (2023)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/stoma.v20i2.44009

Abstract

Root caries is common in the elderly, usually detected late, involving enamel, cementum, and dentin below the cementoenamel junction (CEJ). Remineralisation is used to treat it when cavitation has not occurred. Bioactive glass triggers remineralisation by forming hydroxycarbonate apatite (HCA) at an early stage. Injectable paste preparations are needed to overcome these problems. The use of hyaluronic acid and carrageenan polymers supports the physical and mechanical properties of the paste preparation. The purpose this research to analyse the formation of HCA on the tooth root surface by administering bioactive glass (BAG) injection paste with the addition of seaweed carrageenan. This study was a laboratory experimental study with three research groups, namely treatment group 1 (BAG paste and hyaluronic acid), treatment 2 (BAG paste, hyaluronic acid and carrageenan), and control group (BAG without polymer). All groups were immersed in artificial saliva, incubated at 37oC for six days and then tested by Scanning Electron Microscope (SEM) to see the formation of HCA and FTIR. HCA was quantified by a scoring method according to predetermined score criteria. SEM showed HCA formation in both treatment groups, not the control group. There is HCA formation on the tooth surface by administering BAG injection paste with seaweed carrageenan.