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Aktivitas Bagian Tumbuhan Sirih Hutan (Piper aduncum Linnaeus) yang Berasal dari Lokasi Berbeda dalam Menekan Pertumbuhan Colletotrichum gloeosporioides secara Invitro: english Kumalasari, Lisa; Sulyanti, Eri; Hamid, Hasmiandy
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 2 No. 1 (2018): June 2018
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.2.1.18-27.2018

Abstract

The boiled water of Leaves and inflorescences of spiked pepper (Piper aduncum) is one alternative to control the pathogen of anthracnose (Colletotrichum gloeosporioides) in chili. The purpose of this study was to determine the activity of leaves and inflorescences of spiked pepper from different locations in suppressing the growth of C. gloeosporioides in vitro. The research was carried out in Phytopathology Laboratory of Pests and Plant Diseases Department, Faculty of Agriculture, Universitas Andalas from March to May 2017. The study used a Completely Randomized Design (CRD) with 5 treatments and 5 replications. Data from observations were analyzed by ANOVA and LSD at the level of 5%. The parameters observed were the growth of fungal colony area, wet and dry weight, number of conidia/ml of suspension and conidia germination. The results showed that the application of leaves and inflorescences of spiked pepper from different locations could suppress the growth of C. gloeosporioides in chili in vitro. Boiled water of inflorescences of spiked pepper from Bukit Lampu - Bungus - Padang City became the best treatment because it had the highest ability in suppressing C. gloeosporioides colony area with an effective suppression of 73.5% and reducing the highest germination capacity with an effective suppression of 23.28%. Its ability to reduce wet and dry weight were not different from inflorescences boiled water from Limau Manis, then the ability to suppress the number of conidia was not significantly different from leaves and inflorescences boiled water from Bukit Lampu and Limau Manis.
Evaluasi Isolat Pseudomonad fluoresens Indigenus dari Rizosfir Berbagai Kultivar Tanaman Pisang Sehat di Lahan Endemik Penyakit Layu Fusarium untuk Pengendalian Penyakit Layu Fusarium: english Sulyanti, Eri; Habazar, Trimurti; Husin, Eti Farda; Nasir, Nasril; Dharma, Abdi
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 2 No. 2 (2018): December 2018
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.2.2.87-96.2018

Abstract

Fusarium wilt diseases caused by Fusarium oxysporum f sp cubense (Foc) is the most important diseases on banana in the world. Once Foc is present in the soil, it cannot be eliminated. The aim of research was to evaluate the potential of Pseudomonad fluorescens indigenous to control Fusarium wilt in planta. This experiment was arranged by completely randomized design with 17 treatments and 10 replications. Sixteen isolates of P. fluorescens indigenous had been successly taken from several different cultivars of banana rhizospheres at endemic area of Fusarium wilt in the centre of banana production in West Sumatra that were introduced to banana seedlings cv Cavendish inoculated with Foc. The variable observed were incubation period, percentage of leaf infection, discoloration of pseudostem and the intensity of damaged corms. The result showed that The PfCvP1 isolate (from rhizosphere of Cavendish at low altitude area) was the most effective to inhibit the development of Fusarium wilt: 97.89 % (prolong incubation period), 67.26 % (reduced infected leaves), 63.63 % (reduced damaged corm), and 72.62 % (reduced disclorotion of pseudostem) and increased plant growth.
Aktivitas Air Rebusan Beberapa Kulit Jeruk (Citrus spp) untuk Menekan Pertumbuhan Colletotrichum gloeosporioides pada Tanaman Buah Naga secara In Vitro: Activity of Orange Peel Decoction (Citrus spp) to Suppress the Growth of Colletotrichum gloeosporioides on Dragon Fruit Plants In vitro Sulyanti, Eri; Yaherwandi, Yaherwandi; Ulindari, Restu Monika
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 3 No. 2 (2019): December 2019
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.3.2.56-64.2019

Abstract

Penelitian bertujuan untuk mendapatkan air rebusan kulit jeruk yang efektif dalam menekan pertumbuhan patogen Colletotrichum gloeosporioides penz. & Sacc. penyebab penyakit antraknosa pada tanaman buah naga (Hycoleceurus polyrhizus, L.) secara in vitro. Hasil penelitian diharapkan dapat membantu petani dalam mengendalikan penyakit antraknosa pada tanaman buah naga, serta mampu mempertahankan kualitas dan kuantitas dalam memproduksi buah naga. Penelitian ini dilaksanakan di laboratorium Fitopatologi Fakultas Pertanian Universitas Andalas dengan Rancangan Acak Lengkap, 6 perlakuan dan 5 ulangan. Perlakuannya adalah air rebusan dari beberapa kulit jenis jeruk dengan konsentrasi 5 g/100 ml, diantaranya: A = Tanpa perlakuan (kontrol); B = Kulit jeruk purut (Citrus histrix DC); C = Kulit jeruk manis (Citrus sinensis L.); D = Kulit jeruk nipis (Citrus aurantifolia); E = Kulit jeruk kasturi(Citrus madurensis Lour.) ; F = Pestisida berbahan aktif (tebukonazol, dengan dosis anjuran 2 g/100 ml, sebagai pembanding). Data yang diperoleh dianalisis secara sidik ragam (uji F) dan jika berbeda nyata dilanjutkan dengan (Least Significance Different) LSD pada taraf nyata 5%. Hasil penelitian menunjukkan bahwa dari perlakuan beberapa kulit jeruk dapat menekan pertumbuhan jamur C. gloeosporioides in vitro. Perlakuan air rebusan kulit jeruk manis adalah yang paling baik dengan efektivitas penekanan: luas koloni 76,86%, jumlah konidia 94,58%, dan daya perkecambahan konidia 38,18%. Namun efektivitasnya masih rendah jika dibandingkan dengan penggunaan pestisida berbahan aktif tebukonazol lebih efektif 100%.
Activity of Castor Bean Leaves Extract (Ricinus Communis Linnaeus) to Development of Nematode Meloidogyne Spp. on Tomato Plant (Lycopersicum esculentum Miller) Oktavia, Elisa; Winarto, Winarto; Sulyanti, Eri
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 5 No. 1 (2021): June 2021
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.5.1.34-45.2021

Abstract

Meloidogyne spp. is one of the nematodes that cause root-knot on tomato plants. The botanical nematicide that potentially suppresses the nematode is castor bean leaves (Ricinus communis Linnaeus) extract. This research aimed to get the lethal concentration (LC50 and LC95) of castor bean leaves extract against nematode larvae in-vitro and their effect on the development of root-knot nematode (Meloidogyne spp.) in-planta on tomato plant. The research was carried out in Plant Pest and Disease Laboratory and Greenhouse of Agriculture Faculty, Universitas Andalas, using a completely randomized design (CRD) with two stages; in-vitro and in-planta. The research was used the experiment method in-vitro testing consists of six treatments and five replications, and in-planta testing consists of three treatments and nine replications. The result showed that the application of castor bean leaves extracts invitro can suppress Meloidogyne spp. larvae with LC50 by 0,27% and LC95 by 0,87%. The application of castor bean leaves extract in-planta with treatment concentration 2xLC95 (1,74%) showed effectiveness by 74,69% against the developments of Meloidogyne spp. on tomato plant roots.
The Effectiveness of Ginger Rhizome Extract to Inhibit the Growth of Sclerotium rolfsii in Peanut In-vitro: Potensi Ekstrak Rimpang Jahe dalam Menghambat Pertumbuhan Sclerotium rolfsii pada Kacang Tanah secara In Vitro Syafitri, Syafitri; Sulyanti, Eri; Arneti, Arneti; Swandi, Fradilla
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 6 No. 1 (2022): June 2022
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.6.1.54-63.2022

Abstract

Sclerotium rolfsii is a fungus that causes stem rot disease in peanuts which causes losses of up to 59%. One technique of controlling the S. rolfsii is using a botanical fungicide, such as ginger (Zingiber officinale Rosc.) rhizome extract. This study aimed to determine the proper concentration of ginger rhizome extract to control S.rolfsii, the causes of stem rot, and damping-off diseases in peanut in-vitro. This study used a completely randomized design with five treatments and five replications. The treatments were arranged in the concentration of 0, 4%, 6%, 8%, and 10% by in-vitro. The data were analyzed using ANOVA, followed by an LSD of 5%. The results showed that ginger rhizome extract could suppress the growth of S. rolfsii, which causes stem rot disease in peanut plants. Ginger rhizome extract reduced the thickness of the colonies, suppressed colony expansion, reduced the wet and dry weight of the colonies, slowed the appearance of Sclerotia, and reduced the number of Sclerotia formed. The higher concentration, the higher the emphasis on S. rolfsii. Ginger rhizome extract at a concentration of 10% can inhibit colony growth by 81.63%, inhibit the formation of sclerotia by 100%, reduce the wet weight of the colony to 66.88% and the dry weight of the colony to 44.11% and inhibit the formation of Sclerotia reaching 100%.
Eksplorasi dan Karakterisasi Rizobakteri untuk Mengendalikan Jamur Fusarium fujikuroi secara In vitro Rahma, Zola Puti; Rahma, Haliatur; Sulyanti, Eri
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 8 No. 2 (2024): December 2024
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.8.2.123-137.2024

Abstract

Rhizobacteria is bacteria that found in the root zone of plants. Rhizobacteria can act as biocontrol agents against Fusarium fujikuroi, the fungus that causes bakanae disease in rice plants. This study aimed to obtain rhizobacteria isolates from the roots of rice plants and to determine their characteristics as biocontrol agents against F. fujikuroi in vitro. This research consisted of two stages: 1) Exploration of rhizobacteria from the roots of rice plants in Padang City, West Sumatera, Indonesia and 2) Antagonistic character of Rhizobacteria against F. fujikuroi in vitro including: antagonistic test, hydrolytic enzyme production test (protease, amylase, and chitinase), and HCN compound production test. Based on the research, 32 rhizobacterial isolates were obtained as candidates for biological agents. All rhizobacterial isolates can suppress the growth of Fusarium fujikuroi in vitro with an inhibition percentage of 19.43-73.53%. The 4 best isolates in suppressing the growth of Fusarium fujikuroi are Kr 2.2 from Kuranji, KG 2.2 from Korong Gadang, PA 2.1 and PA 1.2 from Pasar Ambacang with an inhibition percentage of 60.39-73.53%. The four isolates are also able to produce hydrolysis enzymes (protease, amylase and chitinase) and HCN compounds