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All Journal HAYATI Journal of Biosciences JFMR (Journal of Fisheries and Marine Research)
Syahidah, Dewi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Engineering Environmental Science

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Isolation and Identification of Vibrio parahaemolyticus Bacteria in Bottlenose Dolphins (Tursiop truncates) in Kendal Conservation Pond, Central Java Nafiqoh, Nunak; Setiadi, Setiadi; Novita, Hessy; Lusiastuti, Angela Mariana; Indrawati, Agustin; Elmanaviean; Jannah, Siti Nur; Gardenia, Lila; Penataseputro, Tanjung; Andriyono, Sapto; Ningrum, Siti Gusti; Oktaviani, Dian; Syahidah, Dewi; Wattiheluw, Muhammad Subhan; Purwaningsih, Uni
HAYATI Journal of Biosciences Vol. 31 No. 5 (2024): September 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.5.811-819

Abstract

Aquatic mammals in Indonesia are officially protected. However, there is a lack of research on these mammals, particularly in relation to potential disease-causing bacteria. A study was conducted in a conservation pond to address this gap, where swab samples were taken from the blowholes of aquatic mammals. The focus was on identifying bacteria that could potentially cause infectious diseases in these animals. The results revealed V. parahaemolyticus bacterial isolates, which showed a 98% similarity to the registered V. parahaemolyticus in NCBI. These bacterial isolates exhibited hemolysin properties and demonstrated resistance to trimethoprim, streptomycin, cephalothin, and penicillin antibiotics.
HISTOPATHOLOGY of MIXED INFECTIONS in REDCLAW CRAYFISH (Cherax quadricarinatus) TISSUES Syahidah, Dewi; Owens, Leigh
JFMR (Journal of Fisheries and Marine Research) Vol. 4 No. 2 (2020): JFMR
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2020.004.02.4

Abstract

Redclaw crayfish (C. quadricarinatus) diseases have become an important issue with a number of reports from farms in Australia since the 1990s. About 30 Adult crayfish were housed at SVBS JCU and were given mixed food as required for 3wks. Crayfish samples were preserved at D21 by injecting the cephalothorax and abdominal muscles with Davidson’s fixative (10mL of Davidson per 1g crayfish). Histological examination was conducted by longitudinally slicing the cephalothorax, abdomen, and gill tissues at approximately 2-3mm thickness to obtain separate histological sections of the tissues. Sections were cut at 5µm and stained with Mayer’s hematoxylin and eosin (H&E), Gram-Twort, Periodic Acid-Schiff (PAS), and phosphotungstic acid-eosin. An Olympus EC microscope was used to view the sections and the photographs were taken using QCapture.Pro. The results showed melanisation in the hepatopancreatic tissues, hemolymph and gills; Haemocytic aggregation in the hemolymph; Fungal hyphae in the gills; Multiple nodule formation surrounded by melanisation with continued haemocytic aggregation and whirling, early stage of catabolism and bacterial colonies were identified in the muscles. In conclusion, histopathological analyses in this study that redclaw crayfish is susceptible for mixed infection from bacteria, fungi, and virus.
Co-Authors Agustin Indrawati ANGELA MARIANA LUSIASTUTI Elmanaviean Lila Gardenia Nafiqoh, Nunak Novita, Hessy Oktaviani, Dian Owens, Leigh Penataseputro, Tanjung Sapto Andriyono Setiadi Setiadi Siti Gusti Ningrum, Siti Gusti Siti Nur Jannah Uni Purwaningsih Wattiheluw, Muhammad Subhan