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All Journal Sainteks Jurnal Periodic Jurusan Kimia UNP Jurnal Sumberdaya Hayati CHEESA: Chemical Engineering Research Articles Eksakta : Berkala Ilmiah Bidang MIPA Pelita Eksakta Afeksi: Jurnal Penelitian dan Evaluasi Pendidikan BIOCHEPHY: Journal of Science Education Journal of Multidisciplinary Inquiry in Science, Technology and Educational Research
Parbuntari, Hesty
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Identification of Flavonoid Compounds in Ethanol Extract of Majapahit plant (Crescentia cujete) Leaves and their Potential as Anticancer Martha, Rahma Diyan; Fatimah, Fatimah; Danar, Danar; Parbuntari, Hesty
CHEESA: Chemical Engineering Research Articles Vol. 6 No. 1 (2023)
Publisher : Universitas PGRI Madiun

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25273/cheesa.v6i1.14056.34-48

Abstract

The Majapahit plant is commonly found in Indonesia but is rarely used due to a lack of information about its potential. One of the secondary metabolites commonly found in this plant are flavonoids. Therefore, this study aims to determine the distribution of flavonoid compounds in the Majapahit plant, particularly their potential as anticancer activty. Leaf material from Majapahit plants was extracted using the maceration technique, while the flavonoid compounds in the extract were identified using LCMS (Shimadzu LCMS-8040 LC/MS). The identification results showed that about 97 compounds were detected, including 14 flavonoids. The flavonoid compounds found include Quercetin, Chlorogenic acid, Kaempferol 3-O rhamnoside, Acacetin 7-rutinoside, Fortunellin, Kaempferol 3-[6"-(3-hydroxy-3-methyl glutarl) glucoside], Didymin, Diosmin, Hesperidin, Rutin, Citrusoside C, Citrusoside D, Narirutin 4'-glucoside, and Kaempferol 3-[6"-(3-hydroxy-3-methylglutaryl)glucoside] -7-glucoside. The highest composition of the identified flavonoid compounds was found in Kaempferol 3-O rhamnoside, with a 3.90%
The Antibacterial Potential of Ethyl Acetate Fraction from Plectranthus amboinicus Leaves and Identification of Active Compounds Using LC-MS Martha, Rahma Diyan; Safitri, Yunita Diyah; Lestari, Nasa Bela Dwi; Danar, Danar; Parbuntari, Hesty; Maida, Margarita Claudya; Muadifah, Afidatul; Huda, Choirul
CHEESA: Chemical Engineering Research Articles Vol. 7 No. 1 (2024)
Publisher : Universitas PGRI Madiun

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25273/cheesa.v7i1.13826.15-23

Abstract

The Indian Borage plant (Plectranthus amboinicus) is a traditional medicinal ingredient in Indonesia, containing flavonoids, alkaloids, polyphenols, saponins, and essential oils. Therefore, this study aimed to determine the antibacterial activity of P. amboinicus leaves ethyl acetate fraction using the disc diffusion method and identify the composition with LC-MS. The antibacterial activity test was conducted with concentration variations of 15%, 20%, and 25% with Chloramphenicol 500 mg and 5% DMSO as a positive and negative control, respectively. The results showed that the ethyl acetate fraction at a concentration of 20% had the largest inhibition zone diameter, with an average of 30 mm. The LC-MS results identified 115 compounds, and the highest concentration values were found in kaempferol 3-(6”-caffeoylglucoside) and kaempferol 3-glucosyl-(1→2) galactosyl-(1→2)-glucoside, with a composition of 3.26109% and 3.26141%, respectively.
Identifikasi Senyawa Metabolit Sekunder pada Daun Salam (Syzygium polyanthum) Erwan, Meriska Octavia; Parbuntari, Hesty
Periodic Vol 12, No 3 (2023): PERIODIC
Publisher : Departemen Kimia FMIPA UNP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/periodic.v12i3.118432

Abstract

Syzygium polyanthum or bay leaf is a plant that belongs to the Myrataceae family. Almost all parts of this plant can be used, such as roots, bark, leaves, flowers, fruit, and young shoots. But the part that is most often consumed is the leaves, which are widely used traditionally as a remedy for stomach aches, overcoming gout, high cholesterol, stroke, stomach ulcers, and improving blood circulation. In this study, identification of secondary metabolites contained in Syzygium polyanthum was carried out. The results of the identification indicate that Syzygium polyanthum contains secondary metabolites such as flavonoids, alkaloids, terpenoids, and saponins.
Skrining Fitokimia Ekstrak Metanol Dari Daun Jati Belanda (Guazuma ulmifolia) Yuliandari, Fadhira; Parbuntari, Hesty
Periodic Vol 12, No 3 (2023): PERIODIC
Publisher : Departemen Kimia FMIPA UNP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/periodic.v12i3.118434

Abstract

Dutch teak (Guazuma ulmifolia) is a plant that belongs to the family Sterculiaceae and grows in Ecuador, Panamaa, and other American Latin countries. This Dutch teak is found in bushes, as well as in forests. This Dutch teak plant is often the most needed for health, especially the leaves. Dutch teak leaves have been used as traditional medicine to maintain a healthy body, lose weight as a slimming herb, to lowers cholesterol levels in the body. Research has been done phytochemical screening to determine the secondary metabolites contained in Guazuma ulmifolia. Phytochemicals test results showed that Guazuma ulmifolia contains secondary metabolites in the form of flavonoids, steroids, alkaloid, and saponins.
Adsorpsi Ion Ca2+ Menggunakan Adsorben Silika GPTMS Termodifikasi Sulfonat Putri, Reza Athia; Oktavia, Budhi; Alizar, Alizar; Parbuntari, Hesty
Periodic Vol 13, No 2 (2024): PERIODIC
Publisher : Departemen Kimia FMIPA UNP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/periodic.v13i2.124505

Abstract

Silica is a metal oxide compound that is widely found in nature and is used as an adsorbent. Silica has the ability to adsorb ions because it has two groups of active sites that can be modified, namely siloxane (Si-O-Si) and silanol (Si-OH) groups. This modification was made to increase the ability of silica to absorb ions, one of which is the metal ion Ca2+. Silica modification was carried out by adding a bridging compound, namely glycidoxypropyltrimethoxysilane (GPTMS). The purpose of this research was to determine the optimum conditions of pH, contact time, and adsorption concentration of Ca2+ metal ions on sulfonate-modified GPTMS silica using batch method. The filtrate resulting from adsorption was analyzed using an atomic absorption spectrophotometer (AAS). The results showed that the optimum conditions for the adsorption of Ca2+ metal ions by sulfonate-modified GPTMS silica occurred at pH 5, contact time of 60 minutes, and concentration of 20 ppm. The adsorption process of Ca2+ metal ions by sulfonate-modified GPTMS silica follows the langmuir isotherm equation with an absorption capacity of 2.7824 mg/g. The results of the comparison of the absorption capacity of silica after modification obtained greater results than before modification, where the absorption capacity after modification was 2.225375 mg/g with an absorption percentage of 57.17%, before modification the absorption capacity was 1.677275 mg/g, and the absorption percentage was 43.07%.
Skrining Fitokimia Ekstrak Metanol Buah Labu Siam Maulidina, Freshilla; Parbuntari, Hesty
Periodic Vol 12, No 3 (2023): PERIODIC
Publisher : Departemen Kimia FMIPA UNP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/periodic.v12i3.118448

Abstract

Sechium edule. or chayote is a plant that belongs to the Cucurbitaceae family. Almost all parts of this plant can be consumed, one of which is the fruits. chayote can be cooked as a vegetable and is a good source of vitamin C . In this study, phytochemical screening was performed to identify secondary metabolites containing in Musa x paradisiaca L. The results of phytochemical screening showed that Sechium edule. contained secondary metabolites of flavonoids, steroids and saponins, but did not contain alkaloids and terpenoids.
Pengaruh Proses Pengeringan Terhadap Kandungan Fitokimia Jamur Tiram Putih (Pleurotus ostreatus L.) Azura, Nur; Parbuntari, Hesty
Periodic Vol 13, No 2 (2024): PERIODIC
Publisher : Departemen Kimia FMIPA UNP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/periodic.v13i2.123966

Abstract

Pleurotus ostreatus or oyster mushroom is a fungi that usually consumed by people because they have a lot of nutrients and contains bioactive secondary metabolites. However, some bioactive compounds will be damaged if the sample is trough the drying process. The purpose of this study was to know the effect of the drying process on the content of bioactive compounds from oyster mushroom (Pluerotus ostreatus). The results showed a different in the content of bioactive compounds from dried and orginal Pluerotus ostreatus.. Original Pluerotus ostreatus contain bioactive secondary metabolites of alkaloids, steroids, flavonoids and saponins. While dried Pluerotus ostreatus contain alkaloids, steroids, saponin and do not contained flavonoid. Both of sample do not contained terpenoid.
Identifikasi Senyawa Flavonoid Daun Jinten (Plectranthus amboinicus) Menggunakan Liquid Chromatography-Mass Spectrometry (LC-MS) dan Potensinya sebagai Antikanker Secara In-Vitro Martha, Rahma Diyan; Danar, Danar; Safitri, Yunita Diyah; Parbuntari, Hesty
Sainteks Vol 21, No 1 (2024): April
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/sainteks.v21i1.21171

Abstract

Penelitian ini mengungkapkan potensi senyawa flavonoid dalam daun jinten (Plectranthus amboinicus) sebagai agen antikanker. Melalui identifikasi menggunakan Liquid Chromatography-Mass Spectrometry (LC-MS), ditemukan delapan senyawa flavonoid, termasuk Kaempferol 3-(5''-feruloylapioside), Kaempferol 3-(6''-caffeoylglucoside), dan Kaempferol 3-glucosyl-(1 2)-galactosyl-(1 2)-glucoside. Senyawa-senyawa ini telah terbukti memiliki potensi antikanker melalui penelitian sebelumnya. Metode LC-MS dipilih untuk identifikasi senyawa karena memberikan data yang informatif, termasuk informasi tentang massa molekul senyawa yang teridentifikasi. Proses penelitian melibatkan beberapa langkah, mulai dari pembuatan simplisia daun jinten, pembuatan ekstrak melalui maserasi, fraksinasi, hingga identifikasi senyawa menggunakan LC-MS. Temuan ini mengindikasikan bahwa daun jinten memiliki potensi sebagai sumber senyawa antikanker yang bernilai, yang dapat menjadi dasar untuk penelitian lebih lanjut dalam memanfaatkannya dalam terapi kanker.
Analisis In Vitro Aktivitas Antioksidan Ekstrak Aseton dari Ranting Horsfieldia macrothyrsa Menggunakan Beragam Metode: In Vitro Analysis of Antioxidant Activity of Horsfieldia macrothyrsa Twig Acetone Extract Using Various Methods Asnah, Nur; Megawati, Megawati; Parbuntari, Hesty
Jurnal Sumberdaya Hayati Vol. 10 No. 2 (2024): 2024
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.10.2.48-53

Abstract

Horsfieldia macrothyrsa (Miq) Warb. is a plant that contains secondary metabolite compounds that can act as antioxidants. This research aims to determine the antioxidant potential of the acetone extract of the H. macrothyrsa plant by looking at the IC50 value. Twigs of the H. macrothrysa plant were extracted using the maceration method using acetone solvent. The results of the antioxidant activity test using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method showed very strong antioxidant activity with an IC50 value of 6.348 µg/ml whereas the IC50 value of the standard antioxidant quercetin is and quercetin 1.261 µg/ml. In antioxidant testing using the ABTS (2,2-azinobis (3-ethylbenzothiazoline-6-sulfonate) method, an IC50 value of 12,772 µg/ml was obtained and FRAP (Ferric Reducing Antioxidant Power) obtained an IC50 value of 7,511 µg/ml. Based on the results, it can be concluded that the acetone extract of H. macrothrysa plant twigs has very strong antioxidant activity towards reducing DPPH (2,2-diphenyl-1-picrylhydrazyl) compared to using the ABTS and FRAP methods.
Identifikasi dan Kuantifikasi Kadar Protein pada Putih Telur Bebek (Anas domesticus) Yulistia, Siska; Parbuntari, Hesty
Journal of Multidisciplinary Inquiry in Science, Technology and Educational Research Vol. 1 No. 4 (2024): AGUSTUS-OKTOBER 2024
Publisher : UNIVERSITAS SERAMBI MEKKAH

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32672/mister.v1i4.2132

Abstract

Proteins found in animal foods such as eggs are said to be perfect proteins. One example of high protein is duck eggs. In this practicum, qualitative analysis of protein was carried out using the biuret color reagent test, while quantitative analysis was carried out using UV-Vis spectrophotometry. The results of the experiment showed that the protein content of the duck egg white was positive for protein, which was indicated by the presence of a purple color. In qualitative analysis with UV-Vis spectrophotometry method with variations in the concentration of standard solutions, namely 0.2, 0.4, 0.6, 0.8, 1 mg/mL. The maximum wavelength obtained at a concentration of 0.6 mg/ml is 815 nm.
Co-Authors Aini, Syamsi Alizar, Alizar Annisa Annisa Asnah, Nur Azura, Nur Azzahra, Wenalda Hanifah Budhi Oktavia Danar Danar Deski Beri Desy Kurniawati Erwan, Meriska Octavia fatimah Fatimah Febrianti, Latifah Intan Firdaus, Salsabila Kiki Amelia, Kiki Lestari, Nasa Bela Dwi Mahmud Mahmud Maida, Margarita Claudya Martha, Rahma Dyan Maulidina, Freshilla Megawati - Muadifah, Afidatul Putri, Reza Athia Rahma Diyan Martha Safitri, Yunita Diyah Saputra, Anwar Winnugroho Wiratman, Manfaluthy Hakim, Tiara Aninditha, Aru W. Sudoyo, Joedo Prihartono Yuliandari, Fadhira Yulistia, Siska