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PENGUJIAN FITOKIMIA DAN PENENTUAN PARAMETER KINETIK ENZIM DENGAN PENAMBAHAN EKSTRAK INFUSA AIR SELEDRI Rahma Diyan Martha; Atiqoh Zummah
Jurnal Wiyata Penelitian Sains dan Kesehatan Vol 5, No 2 (2018)
Publisher : LP2M IIK (Lembaga Penelitian dan Pengabdian Masyarakat Institut Ilmu Kesehatan) Bhakti Wiy

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Abstract

Latar belakang: Hiperurisemia merupakan kelainan metabolisme karena produksi asam urat yang berlebih. Prevalensi hiperurisemia di Indonesia diperkirakan antara 2,3-17,6%, sehingga penelitian mengenai obat antihiperuresemia sangat dibutuhkan saat ini. Tujuan: untuk mengetahui aktivitas infusa batang seledri terhadap parameter kinetic enzim xantin oksidase. Metode: spektrofotometri UV untuk mengukur kadar asam urat. Hasil: pengujian kandungan senyawa bahan aktif menunjukkan hasil positif pada pengujian flavonoid. Parameter kinetik enzim Km dan Vmaks menunjukkan perubahan nilai dengan adanya penambahan infusa batang seledri. Nilai Km enzim adalah sebesar 90,00 ppm, dan nilai Vmaks enzim sebesar 3,51 U/mL sebelum adanya penambahan ekstrak air batang seledri dan nilai Km dan Vmaks berubah menjadi 125,42 ppm dan 2,97 U/mL dengan adanya penambahan ekstrak infusa air batang seledri. Simpulan dan saran: pengujian kandungan senyawa bahan aktif menunjukkan hasil positif pada pengujian flavonoid, sedangkan uji parameter kinetik enzim menunjukkan kenaikan Km dan Vmaks menunjukkan penurunan  dengan adanya penambahan ekstrak infusa air batang seledri.
PENENTUAN PARAMETER KINETIK ENZIM XANTIN OKSIDASE TERINHIBISI EKSTRAK SELEDRI BERDASARKAN METODE GRAFIK LINEWEAVER-BURK DAN LANGMUIR ATIQOH ZUMMAH; RAHMA DIYAN MARTHA
Jurnal Wiyata Penelitian Sains dan Kesehatan Vol 7, No 1 (2020)
Publisher : LP2M IIK (Lembaga Penelitian dan Pengabdian Masyarakat Institut Ilmu Kesehatan) Bhakti Wiy

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Abstract

Latar belakang: Seledri merupakan tanaman obat yang diketahui mampu menghambat aktivitas enzim xantin oksidase yang berperan dalam pembentukan asam urat. Prevalensi penyakit asam urat (gout) yang tinggi di Indonesia, yaitu 1,6-13,6% per seribu penduduk membuat penelitian tentang sifat penghambatan terbentuknya produk asam urat dalam tubuh sangat diperlukan. Tujuan: mengetahui nilai parameter kinetik enzim xantin oksidase dengan adanya penambahan ekstrak seledri. Metode: Ekstrak seledri dari daun dan batang diperoleh menggunakan metode soxhletasi dan pelarut hidroetanol. Penentuan parameter kinetik enzim dilakukan dengan metode grafik linear Lineweaver-Burk dan Langmuir. Hasil: Ekstrak hidroetanol daun seledri menunjukkan inhibisi terbaik sebesar 86.86% (konsentrasi 30%) dan ekstrak hidroetanol batang seledri menunjukkan inhibisi terbaik sebesar 87.71% (konsentrasi 40%). Penambahan ekstrak daun seledri pada enzim memberikan nilai Km sebesar 27.74 ppm dan Vmaks sebesar 3,55 U/mL pada metode grafik Lineweaver-Burk, sedangkan pada metode grafik Langmuir nilai Km mencapai 327.66 ppm dan Vmax sebesar 6.45 U/mL. Penambahan ekstrak batang seledri menghasilkan nilai Km sebesar 145.24 ppm dan Vmax sebesar 4,55 U/mL pada metode grafik Lineweaver-Burk, sementara pada metode grafik Langmuir nilai Km sebesar 321,09 ppm dan Vmaks sebesar 5.21 U/mL. Kesimpulan: Metode grafik Langmuir lebih sesuai digunakan untuk penentuan parameter kinetik dalam inhibisi enzim xantin oksidase oleh ekstrak seledri.
SINTESIS ANALOG KURKUMIN 2,6-BIS-(E)-4-HIDROKSI-3-METOKSI BENZILIDIN)-SIKLOHEKSA-1-ON BERBAHAN DASAR VANILIN DENGAN KATALIS HCl Rahma Diyan Martha; Tutik Dwi Wahyuningsih; Chairil Anwar
Jurnal Penelitian Saintek Vol 25, No 2 (2020)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v25i2.34106

Abstract

Tujuan penelitian ini memodifikasi gugus β-diketon sehingga dapat meningkatkan stabilitas dari analog kurkumin.Metode yang digunakan yaitu sintesis senyawa analog kurkumin berbahan dasar vanillin sebagai sumber benzaldehida dan sikloheksanon sebagai sumber keton dengan katalis asam (HCl). Dianalisis dengan KLT dan instrumentasi FTIR,direct-MS,1H NMR dan 13C NMR. Dari hasil analisis titik leleh, KLT, FTIR, direct-MS, 1H- dan 13C-NMR, bahwa senyawa target telah terbentuk berupa padatan warna kuning kehijauan, memiliki titik leleh 210-212 °C dan rendemen 53,00% serta hasil KLT terdapat spot baru yang berfluorosensi. Sehingga, dapat disimpulkan bahwa senyawa analog kurkumin (2,6-bis-((E)-4-hidroksi-3-metoksibenzilidin)-sikloheksa-1-on) dapat disintesis melalui kondensasi aldol menggunakan starting material vanilin sebagai sumber benzaldehida dan sikloheksanon sebagai sumber keton dengan katalis asam (HCl).THE SYNTHESIS OF CURCUMINE ANALOGUE 2,6-BIS-(E)-4-HIDROKSI-3-METOKSI BENZILIDIN)-SIKLOHEKSA-1-ON FROM VANILIN WITH HYDROCLORIDE ACID CATALYSTThe aim of this study was to modify the β-dicetone group so as to increase the stability of the curcumin analogue. The method used is the synthesis of a curcumin analogue compound based on vanillin as a source of benzaldehyde and cyclohexanone as a source of ketones with an acid catalyst (HCl). Analyzed by TLC and FTIR instrumentation, direct-MS, 1H NMR and 13C NMR. From the analysis of melting point, TLC, FTIR, direct-MS, 1H- and 13C-NMR, that the target compound has been formed in the form of a greenish yellow solid, has a melting point of 210-212 ° C and a yield of 53.00% and the results of TLC are new fluorescent spot. Thus, it can be concluded that the curcumin analogue compound (2,6-bis - ((E) -4-hydroxy-3-methoxy benzylidine) -cyclohexa-1-on) can be synthesized through aldol condensation using vanillin as a source of benzaldehyde and cyclohexanone. as a source of ketones with an acid catalyst (HCl).
Analisis toksisitas dan potensi antikanker ekstrak metanol daun Majapahit (Crescentia cujete) dengan metode Brine Shrimp Lethality Test Fatimah Fatimah; Rahma Diyan Martha; Danar Danar
Jurnal Penelitian Saintek Vol 27, No 1 (2022)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v1i1.43889

Abstract

Tanaman Majapahit (Crescentia cujete) merupakan salah satu tanaman di Indonesia yang diketahui memiliki senyawa fenolat, saponin, tanin, alkaloid, dan terpenoid. Beberapa jenis senyawa yang termasuk kedalam golongan fenol diketahui memiliki aktivitas antikanker. Pada penelitian ini dilakukan untuk mengetahui toksisitas ekstrak metanol daun Majapahit menggunakan metode Brine Shrimp Lethality Test (BSLT). Pada uji toksisitas menggunakan metode BSLT digunakan lima konsentrasi ekstrak metanol daun Majapahit, yaitu; 500, 400, 300, 200, dan 100 ppm. Ekstrak tersebut kemudian di ujikan pada larva udang Artemia salina L. umur 48 jam. Pada tiap konsentrasi digunakan 10 ekor larva dengan tiga kali pengulangan. Data kematian larva tiap konsentrasi, kemudian di lakukan analiais probit untuk menentukan nilai LC50. Berdasarkan hasil analisis probit dari kematian larva udang pada tiap konsentrasi ekstrak, dapat diketahui bahwa nilai LC50 ekstrak metanol daun Majapahit adalah 642,877ppm. Oleh karena itu, dapat dikatakan bahwa ekstrak bersifat toksik dan berpotensi dikembangkan sebagai antikanker karena memiliki nilai LC50 1000 ppm.Analysis of toxicity and anti-cancer potential of Majapahit (Crescentia cujete) leaf methanol extract using Brine Shrimp Lethality Test methodThe Majapahit plant (Crescentia cujete) is one of the plants in Indonesia which is known to have phenolic compounds, saponins, tannins, alkaloids, and terpenoids. Several types of compounds belonging to the phenol group are known to have anticancer activity. The study aimed to determine the toxicity of the methanol extract of the leaves of Majapahit using the Brine Shrimp Lethality Test (BSLT) method. Toxicity test was done using five concentrations of Majapahit leaf methanol extract, there were 500, 400, 300, 200, and 100 ppm. The Majapahit extract was then tested on Artemia salina L. shrimp larvae aged 48 hours. At each concentration, ten larvae were used with three repetitions. Larvae mortality data for each concentration of the extract would be used to carried out probit analysis to determine the LC50 value. Based on the results of the probit analysis of the mortality of shrimp larvae at each extract concentration, it could be seen that the LC50 value of the Majapahit leaf methanol extract is 642.877ppm. So, it means that the extract is toxic and has the potential to be developed as an anticancer because it has an LC50 value of 1000 ppm.
Antihyperurisemic Activity of Aqueous Celery Infusion by Xanthine Oxidase Enzyme Inhibition Atiqoh Zummah; Rahma Diyan Martha
Majalah Obat Tradisional Vol 23, No 3 (2018)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (18.388 KB) | DOI: 10.22146/mot.38500

Abstract

Hyperuricemia is a metabolic disorder because of excessive uric acid production. The high prevalence of hyperuresemia in the world, especially in Indonesia makes the research of antihyperurisemia drugs very needed today. The purpose of this study was to determine the activity of water infusion extract from celery stems and leaves to inhibited xanthine oxidase enzyme. Xanthine oxidase enzyme plays a role in change of hypoxanthine to xanthine and then becomes uric acid. The activity of xanthine oxidase enzyme was determined by UV spectrophotometry method and using xanthine substrate. Absorption at 290 nm indicates the presence of a uric acid product from xanthine oxidation by the xanthine oxidase enzyme. Infused extract which has the smallest absorption at 290 nm showed a better inhibitory ability of the xanthine oxidase enzyme because the change of xanthine substrate to uric acid is inhibited. The result showed the celery stems water infusion extract had better inhibition activity than celery leaves water infusion extract. The best inhibition percentage of celery stems water infusion was 90,25%, while the celery leaves water infusion was 69.07% both of at 20% concentration. The type inhibition of celery stems water infusion extract to xanthine oxidase enzyme showed an uncompetitive inhibition type because showed change in Km and Vmax values. Km and Vmax values before the addition of water infusion of celery stems were 104.33 ppm and 3.83 U/mL respectively changed to 74.49 ppm and 2.69 U/mL after the addition of extract.
UJI AKTIVITAS ANTIBAKTERI FRAKSI BATANG PEPAYA (Carica Papaya Linn.) TERHADAP BAKTERI Staphylococcus Aureus Kristina Handayani; Amalia Eka Putri; Rahma Diyan Martha
JURNAL FARMASI DAN MAKANAN Vol 4 No 1 (2020): Journal Of Pharmacy and Science
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/jops.v4i1.1446

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Penyakit infeksi merupakan salah satu penyakit yang penting di Indonesia. Penyakit infeksi dapat disebabkan dari mikroorganisme patogen seperti, bakteri Staphylococcus aureus ATCC 25923. Penyakit infeksi dapat diobati dengan pemberian antibiotik, akan tetapi antibiotik sekarang mengalami resistensi. Oleh karena itu, diperlukan alternatif lain seperti batang papaya yang dapat di manfaatkan sebagai antibakteri terhadap S.aureus ATCC 25923. Tujuan penelitian ini adalah untuk mengetahui aktivitas antibakteri terhadap S.aureus ATCC 25923 dan mengetahui nilai KHM dalam fraksi teraktif fraksi batang papaya. Sampel batang papaya diekstraksi menggunakan metode soxhletasi dengan etanol 96% dilanjutkan fraksinasi menggunakan tiga pelarut yaitu aquadestilata, diklorometana dan n-heksan. Kontrol positif yang digunakan adalah klindamisin dan kontrol negatif adalah pelarut yang sesuai. Fraksi teraktif dilakukan pengujian untuk mengetahui Konsentrasi Hambat Minimum (KHM) menggunakan pengamatan secara visual dan spektrofotometer Uv-Vis. Analisa statistik dilakukan dengan Kruskal Wallis dan Mann Whitney. Hasil pengujian aktivitas antibakteri fraksi batang papaya menunjukkan adanya aktivitas antibakteri terhadap S.aureus ATCC 25923. Hasil zona hambat pada fraksi aquadestilata, diklorometana dan n-heksan berturut-turut adalah 20,63±0,28 mm, 17,86±0,18 mm dan 15,33±0,47 mm. Fraksi batang papaya yang memiliki zona hambat paling aktif adalah pada fraksi aquadestilata. Nilai Konsentasi Hambat Minimum fraksi aquadestilata adalah 0,312%.
Uji Toksisitas Ekstrak Etanolik Batang Tanaman Majapahit (Crescentia cujete) Terhadap Artemia salina Leach Rahma Diyan Martha; Fatimah
Sistem Informasi Vol 11 No 1 (2020): Jurnal Photon
Publisher : LPPM Universitas Muhammadiyah Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37859/jp.v11i1.2146

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Cancer is a disease characterized by uncontrolled cell growth and the ability of these cells to attack biological tissue. Various attempts have been made, one of them with treatment to kill cancer cells, but not a few of these efforts actually cause side effects. This fact demands the need for a safe alternative way to treat cancer using natural ingredients. One of the local natural resources that has the potential to treat cancer is Majapahit (Crescentia cujete). In this study, Majapahit (C. cujete) stem extracts were carried out which have anticancer activity. The first stage was screening by conducting a qualitative analysis of the ethanol extract of the stem of the majapahit plant (C. cujete). Furthermore, a toxicity test was carried out against Artemia salina Leach using the Brine Shrimp Lethality Test (BST) method. Phytochemical testing was carried out to check the content of secondary metabolite compounds contained in the majapahit stem extract, and the results were positive for flavonoids, tannins, saponins and phenolics. This data, supported by spectrophotometric tests, proved that the largest concentration of the ethanolic extract of the majapahit (C. cujete) stem was 24.05% tannin. Then the LC50 test from the toxicity test of the active component of majapahit plant stem extract (C. cujete) against Artemia salina Leach showed a concentration of 0.01 µg / mL and 0.04 µg / mL, namely with a percentage below 50%.
Synthesis of Curcumin Derivatives (2.5-Bis((E)-4-Hydroxy-3-Methoxy Benzylidine) Cyclopenta-1-On) from Vanillin and Its Activity Test Against Α-Glucosidase Enzymes Rahma Diyan Martha; Danar Danar; Rini Retnosari
Walisongo Journal of Chemistry Vol 5, No 1 (2022): Walisongo Journal of Chemistry
Publisher : Department of Chemistry Faculty of Science and Technology Walisongo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/wjc.v5i1.8905

Abstract

Synthesis of curcumin analogs (2.5-Bis((E)-4-Hydroxy-3-Methoxy Benzylidine) Cyclopenta-1-On) based on Claisen-Schmidt condensation using an acid catalyst and inhibition of glucosidase enzyme was carried out. The antidiabetic activity of curcumin analogs was carried out by the use of α-glucosidase enzyme from moldy rice (Oryza sativa). The curcumin analogs were synthesized by reacting the vanillin with cyclopentanone.  The structure of all products was confirmed by FTIR, GC-MS, 1H-13C-NMR spectrometers, and the activity enzyme. The results showed the curcumin analogs were yielded in 52.27%, respectively as yellowish-green solid. The inhibition test of the α-glucosidase enzyme showed that the curcumin analogs were the potential to inhibit the α-glucosidase enzyme with an inhibition percentage of about 94.26% in 5 mM.
Anti-Cancer Activity Testing of Cumin (Plectranthus Amboinicus) Ethanol Extract Against Artemia Salina Leach by Using Brine Shrimp Lethality Test (BSLT) Method ika Rachutami; Rahma Diyan Martha; Afidatul Muadifah; Algafari Bakti Manggara
Walisongo Journal of Chemistry Vol 5, No 1 (2022): Walisongo Journal of Chemistry
Publisher : Department of Chemistry Faculty of Science and Technology Walisongo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/wjc.v5i1.9086

Abstract

The purpose of this study was to determine the anticancer activity of cumin leaf extract (Plectranthus amboinicus), which was applied using the Brine Shrimp Lethality Test (BSLT) method to determine the level of acute toxicity (LC50) of the extract against Artemia salina LEACH larvae. Cumin leaf samples were extracted using the maceration method with 96% ethanol. The cumin leaf extract was then tested qualitatively and quantitatively. Toxicity testing used concentrations of 500 ppm, 1000 ppm, 1500 ppm, 2000 ppm, and 2500 ppm, which were given to Artemia salina LEACH larvae for 24 hours. Calculate the LC50 value in this study using probit analysis. The results of qualitative and quantitative testing showed the presence of saponins by 5.20%, tannins by 8.21%, flavonoids by 23.93%, and alkaloids by 4.37%. The results of the acute toxicity test of the extract using the BSLT method showed that there was anticancer activity with the acquisition of LC50 of 1000 ppm, which was 697.99 ppm.
Pelatihan Pembuatan Lilin Aromaterapi Berbasis Minyak Jelantah Rahma Diyan Martha; Fatimah Fatimah; Ayu Insa; Nasa Bella; Sri Wahyuningsih; Danar Danar
Jurnal Kreativitas Pengabdian Kepada Masyarakat (PKM) Vol 5, No 3 (2022): Volume 5 No 3 Maret 2022
Publisher : Universitas Malahayati Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/jkpm.v5i3.5667

Abstract

ABSTRAKMinyak jelantah merupakan minyak goreng yang digunakan secara berulang-ulang untuk menggoreng makanan dengan menggunakan minyak goreng yang sama. Penggunaan minyak jelantah tersebut menimbulkan bahaya bagi tubuh kita dan pembuangan minyak jelantah secara sembarangan dapat menimbulkan dampak negatif bagi lingkungan maupun perairan. Permasalah ini ditemukan di Desa Bendiljati Wetan Kecamatan Sumbergempol Kabupaten Tulungagung. Tujuan pengabdian kepada masyarakat ini adalah memberikan sosialisasi dan pelatihan (edukasi) kepada ibu-ibu rumah tangga terkait minyak jelantah dan pemanfaatan kembali limbah tersebut. Metode yang digunakan dalam kegiatan ini adalah ceramah dan tanya jawab. Evaluasi dalam kegiatan ini dengan cara membagikan angket berupa pretest dan postest yang dibagikan sebelum dan sesudah kegiatan. Hasil kegiatan ini menunjukkan bahwa penyuluhan dan pelatihan tentang pembuatan lilin dari minyak jelantah telah memberikan informasi dan pengetahuan baru kepada ibu-ibu rumah tangga Desa Bendiljati Wetan. Kegiatan ini telah dapat diikuti dan dilaksanakan dengan baik oleh para peserta.Kata Kunci: Minyak jelantah, lilin, Ibu-ibu rumah tangga ABSTRACT Used cooking oil is cooking oil that is used repeatedly to fry food using the same cooking oil. Using used cooking oil poses a danger to our bodies, and indiscriminate disposal of used cooking oil can harm the environment and waters. This problem was found in Bendiljati Wetan Village, Sumbergempol District, Tulungagung Regency. This community service aims to provide homemakers with socialization and training (education) regarding used cooking oil and the reuse of the waste. The method used in this activity is lecture and question and answer. This activity evaluation by distributing questionnaires in the form of pretest and post-tests shared before and after the action. This activity indicates that counseling and training on making candles from used cooking oil have provided new information and knowledge to homemakers in Bendiljati Wetan Village. This activity has been followed and carried out well by the participants. Keywords: Used cooking oli, candles, homemaker
Co-Authors - Fatimah Adisyaningrum, Ega Nurgia Aena Laili Afidatul Muadifah Afidatul Muadifah Ahmad Yudianto Algafari Bakti Manggara Amalia Putri Eka Anas Fadli Wijaya Anisa Azizah Annisah Mahanani Aprilia, Chatrina Apriliani, Herlina Arif Santoso Arif Santoso Asmarani Kusumawati Atiqoh Zummah Ayu Insa Ayu Insa Fajri Azriel Restu Azriel Restu Chairil Anwar Choirul Huda Choirul Huda Danar Danar Danar Danar Danar, Danar Dara Pranidya Tilarso Dita Arindea Tunggal Putri Dyah Setyaningrum, Dyah Ega Nurgia Adisyaningrum Ega Nurgia Adisyaningrum Elen Vikelavianis Elen Vikelavianis Ema Krismawar Sari Ema Krismawar Sari Fadila, Natasya Nurul Fajri, Ayu Insa Fatimah Fatimah Fatimah fatimah Fatimah Fatimah Fatimah Fuzianingsih, Eka Novia Hesty Parbuntari ika Rachutami Ika Rachutami Ita Rhosida Ita Rhosida Jalu Prakoso Jalu Prakoso Kartika Arum Wardani Kharisma, Mei Nabila Revina Kholifah, Siti Nurriyatul Kristianto, Sonny Kristina Handayani Kumalasari, Nurul Chamidah Lestari, Nasa Bela Dwi Maida, Margarita Claudya Mashudi Mashudi Maya Diva Maya Diva Siswidiani Muadifah, Afidatul Muadifah, Afidhatul Mutia Hariani Murjanah Mutia Hariani Nurjanah Nasa Bella Ngibad, Khoirul Niken Desi Wulandhari Nurin, Wachidatun Nurjanah, Mutia Hariani Nurul Chamidah Kumalasari Nurul Chamidah Kumalasari Nurul Chamidah Kumalasari Nurul Chamidah Kumalasari Parbuntari, Hesty Putri, Amalia Eka Rahmadani, Anindita Mutiara Rahmawati, Ayu Sintya Citra Ramadhani, Melati Apriliana Retnosari, Rini Rian Anggia Wijaya Rury Eryna Putri Safitri, Yunita Diyah Sefrina Sefrina Siti Awwalul Amanatur Rohmah Siti Nurriyatul Kholifah Sri Wahyuningsih SULASTRI Sulastri Sulastri Tilarso, Dara Pranidya Tutik Dwi Wahyuningsih Ulayya, Nida Asma vifta, rissa laila Widayanti, Sindi Dwi Wimbuh Tri Widodo Winnugroho Wiratman, Manfaluthy Hakim, Tiara Aninditha, Aru W. Sudoyo, Joedo Prihartono Wulandhari, Niken Desi Yuliandari, Fadhira