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Phytochemical Screening and Quantitative Analysis of Coleus arthropurpureus Ethyl Acetate Fraction and Antibacterial Activity Against Staphylococcus aureus Tarigan, Indra Lasmana; Sari, Ayu Kumala; Huda, Choirul; Jovanncha, Czellicya; Muadifah, Afidatul
ALKIMIA Vol 4 No 1 (2020): ALKIMIA
Publisher : SCIENCE AND TECHNOLOGY FACULTY OF UNIVERSITAS ISLAM NEGERI RADEN FATAH PALEMBANG

Staphylococcus aureus (S. aureus) is a pathogenic microbe that is caused by various diseases in humans and animals. Infectious diseases caused by S. aureus in Asia reached 70% in 2007, while in Indonesia reached 23.5%. The plant provided several bioactive compounds that might function as an antibacterial which inhibits both bacterial growth and damaging the cell system and protein synthesis. Coleus arthropurpureus known contains alkaloids and tannins that supposed to be an antibacterial compound. Tannins have antibacterial activity, in general, the mechanism is to damage the bacterial cell membrane and induce the formation of complex compound bonds to enzymes or microbial substrates. This study aims to analyze the bioactive compounds contained in C. arthropurpureus in qualitative and quantitative which have an antimicrobial function using high-performance liquid chromatography (HPLC) in the reverse phase C-18 column and screening of antibacterial activity was carried out by disc diffusion method. The results of both qualitative and quantitative analysis by HPLC has obtained the presence of tannin bioactive compounds (1.48 ppm at a retention time of 2.806 minutes) and alkaloids (1.11 ppm at a retention time of 7.015). Moreover, we verified the diameter of inhibition of growth zone against S. aureus at a concentration of 15% extract was 12.80 mm. It was found that the highest percentage of the bioactive compound in C. arthropurpureus is tannin, and that is might an antibacterial agent.

The Antibacterial Potential of Ethyl Acetate Fraction from Plectranthus amboinicus Leaves and Identification of Active Compounds Using LC-MS Martha, Rahma Diyan; Safitri, Yunita Diyah; Lestari, Nasa Bela Dwi; Danar, Danar; Parbuntari, Hesty; Maida, Margarita Claudya; Muadifah, Afidatul; Huda, Choirul
CHEESA: Chemical Engineering Research Articles Vol. 7 No. 1 (2024)
Publisher : Universitas PGRI Madiun

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25273/cheesa.v7i1.13826.15-23

The Indian Borage plant (Plectranthus amboinicus) is a traditional medicinal ingredient in Indonesia, containing flavonoids, alkaloids, polyphenols, saponins, and essential oils. Therefore, this study aimed to determine the antibacterial activity of P. amboinicus leaves ethyl acetate fraction using the disc diffusion method and identify the composition with LC-MS. The antibacterial activity test was conducted with concentration variations of 15%, 20%, and 25% with Chloramphenicol 500 mg and 5% DMSO as a positive and negative control, respectively. The results showed that the ethyl acetate fraction at a concentration of 20% had the largest inhibition zone diameter, with an average of 30 mm. The LC-MS results identified 115 compounds, and the highest concentration values were found in kaempferol 3-(6”-caffeoylglucoside) and kaempferol 3-glucosyl-(1→2) galactosyl-(1→2)-glucoside, with a composition of 3.26109% and 3.26141%, respectively.

Antibacterial Activity of Ethyl Acetate and Cream Formulation of Coleus atropurpureus leaves Against Staphylococcus aureus Tarigan, Indra Lasmana; Muadifah, Afidatul; Susanto, Nindita Clourisa Clourisa Amaris; Huda, Choirul
Pharmaceutical Journal of Indonesia Vol. 7 No. 1 (2021)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.pji.2021.007.01.1

Infectious diseases caused by Staphylococcus aureus (Sa) bacteria reaching 70% of cases in Asia and can attack and survive in epithelial cells including endothelial cells. Attempts to overcome the infection caused by Sa by giving antibiotics, which work as antibacterial. Myana (Coleus atropurpureus L. Benth) leaves contain several active compounds that might act as antibacterial agents. This study aimed to analyze the antibacterial activity of Coleus Extract in vitro and to evaluate the formulation of the extract cream against the bacteria causing infection, Sa. Coleus leaves ethyl acetate extract contains alkaloids and tannins, the absorbance of Sa at the 3rd hour was getting smaller, from 0.668 to 0.552, this showed that the longer the incubation time of the extract against bacterial inoculation, the more bacteria died. Our cream formulation with criteria: the pH value, homogeneity, spreadability, adhesion, and protection power. Our cream formulation results were then evaluated based on the standard, with criteria: the pH value, homogeneity, spreadability, adhesion, and protection power. It seems that our cream is per the cream standards. It is semi-solid, brownish-white in color, smells typical of Coleus, and pH 6. Moreover, the cream is homogeneous with 5 cm of spreadability, 7 seconds for adhesion, and colorless. Moreover, we found that Coleus extract cream has better antibacterial activity than positive control with a wound closure time of 7-14 days for extract while more than 14 days for positive control.

Antibacterial Activity of Ethyl Acetate and Cream Formulation of Coleus atropurpureus leaves Against Staphylococcus aureus Tarigan, Indra Lasmana; Muadifah, Afidatul; Susanto, Nindita Clourisa Clourisa Amaris; Huda, Choirul
Pharmaceutical Journal of Indonesia Vol. 7 No. 1 (2021)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.pji.2021.007.01.1

Infectious diseases caused by Staphylococcus aureus (Sa) bacteria reaching 70% of cases in Asia and can attack and survive in epithelial cells including endothelial cells. Attempts to overcome the infection caused by Sa by giving antibiotics, which work as antibacterial. Myana (Coleus atropurpureus L. Benth) leaves contain several active compounds that might act as antibacterial agents. This study aimed to analyze the antibacterial activity of Coleus Extract in vitro and to evaluate the formulation of the extract cream against the bacteria causing infection, Sa. Coleus leaves ethyl acetate extract contains alkaloids and tannins, the absorbance of Sa at the 3rd hour was getting smaller, from 0.668 to 0.552, this showed that the longer the incubation time of the extract against bacterial inoculation, the more bacteria died. Our cream formulation with criteria: the pH value, homogeneity, spreadability, adhesion, and protection power. Our cream formulation results were then evaluated based on the standard, with criteria: the pH value, homogeneity, spreadability, adhesion, and protection power. It seems that our cream is per the cream standards. It is semi-solid, brownish-white in color, smells typical of Coleus, and pH 6. Moreover, the cream is homogeneous with 5 cm of spreadability, 7 seconds for adhesion, and colorless. Moreover, we found that Coleus extract cream has better antibacterial activity than positive control with a wound closure time of 7-14 days for extract while more than 14 days for positive control.

Analisis Mutu Aktivitas Antioksidan Fraksi Daun Miana (Coleus atropurpureus L. Benth) Terhadap Masa Simpan Permen Jelly Muadifah, Afidatul; Ima, Ernisa Afidatul; Putri, Amalia Eka
Majalah Farmaseutik Vol 19, No 4 (2023)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/farmaseutik.v19i4.79364

Tanaman miana (Coleus artropurpureus L. Benth) menjadi salah satu sumber antioksidan alami yang dapat menangkal radikal bebas karena mengandung senyawa flavonoid, saponin, tanin, dan alkaloid. Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan fraksi daun miana, mutu fisik permen jelly fraksi daun miana dan umur simpan permen jelly fraksi daun miana berdasarkan laju perubahan kadar air dan aktivitas antioksidan. Fraksi daun miana dibuat variasi konsentrasi yaitu 20 ppm, 40 ppm, dan 60 ppm. Kemudian diuji aktivitas antioksidannya menggunakan metode DPPH dengan asam askorbat sebagai pembanding. Hasil nilai IC50 pada fraksi daun miana terbaik yaitu pada fraksi aquadestilata sebesar 79,943 ppm yang tergolong memiliki aktivitas antioksidan kuat. Kemudian variasi konsentrasi fraksi aquadestilata daun miana dimasukkan kedalam formulasi sediaan permen jelly. Permen jelly fraksi daun miana diukur aktivitas antioksidan dan kadar air setiap 4 hari sekali selama 12 hari dari hari ke 0. Diperoleh nilai IC50 pada sediaan permen jelly dari hari ke-0 sampai ke-12 yaitu 84,065 ppm, 91,238 ppm, 99,971 ppm, 110,335 ppm dan sediaan telah memenuhi persyaratan uji mutu fisik. Kadar air permen jelly fraksi daun miana untuk F1 pada hari ke-0 sampai ke-12 yaitu 11,73; 13,99; 16,91; 18,21. F2 pada hari ke-0 sampai ke-12 yaitu 12,81; 14,01; 16,89; 20,00. F3 pada hari ke-0 sampai ke-12 yaitu 11,97; 14,10; 17,03; 20,00. F4 pada hari ke-0 sampai ke-12 yaitu 11,99; 13,83; 16,87; 19,98. Berdasarkan perubahan kadar air pada permen jelly fraksi daun miana dihitung umur simpannya, didapat rata-rata pada keempat formulasi memiliki umur simpan selama 11 hari.

EDUKASI KIMIA BERBASIS BAHAN ALAM UNTUK KUALITAS HIDUP DESA TANGGUNGGUNUNG TULUNGAGUNG Martha, Rahma Diyan; Danar, Danar; Muadifah, Afidatul; Putri, Amalia Eka; Huda, Choirul; Nurjanah, Mutia Hariani
GEMAKES: Jurnal Pengabdian Kepada Masyarakat Vol. 4 No. 2 (2024): GEMAKES: Jurnal Pengabdian Kepada Masyarakat
Publisher : Politeknik Kesehatan Kemenkes RI Jakarta I

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36082/gemakes.v4i2.1605

Kesehatan dan kualitas hidup masyarakat desa menjadi fokus utama dalam upaya pembangunan sosial yang berkelanjutan. Artikel ini membahas kegiatan pengabdian masyarakat di Desa Tanggunggunung dengan tujuan meningkatkan budaya hidup sehat dan kualitas hidup melalui edukasi kimia berbasis bahan alam. Pengabdian Masyarakat diawali dengan mencatat tantangan kesehatan masyarakat desa, terutama dalam akses terhadap informasi dan praktik kesehatan yang tepat. Selain itu, minimnya pemahaman tentang pentingnya menjaga kesehatan dan pemanfaatan sumber daya alam desa juga menjadi perhatian. Metode pelaksanaan kegiatan pengabdian melibatkan penyuluhan, pre-test dan post-test menggunakan kuesioner, serta analisis statistik. Hasil pengabdian menunjukkan peningkatan signifikan dalam pengetahuan peserta setelah program edukasi, dengan nilai rerata pengetahuan meningkat dari 72,3% menjadi 83,2%. Kesimpulan dari kegiatan pengabdian ini menegaskan pentingnya edukasi dan pemberdayaan masyarakat dalam memahami dan menerapkan pengetahuan kimia berbasis bahan alam untuk meningkatkan kualitas hidup. Saran dari penelitian ini adalah pentingnya pengembangan program edukasi yang komprehensif dan berkelanjutan untuk mengatasi tantangan tersebut.

Variasi Konsentrasi Kombinasi Ekstrak Buah Belimbing Wuluh (Averrhoa bilimbi L.) dan Daun Sirih (Piper betle) Menggunakan Metode Hidroekstraksi Seabagai Antibakteri Seara In Vitro Tilarso, Dara Pranidya; Muadifah, Afidatul; Khusna, Mursyidah Lathifatul
Jurnal Farmasi & Sains Indonesia Vol 7 No 2 (2024)
Publisher : LPPM Sekolah Tinggi Ilmu Farmasi Nusaputera

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52216/jfsi.vol7no2p1-9

Infectious diseases caused by bacteria are diseases that often arise in society. Bacteria that cause infection include Staphylococcus aureus and Escherichia coli. Bacterial growth can be inhibited with antibiotics. However, some microbes have become resistant to antibiotics so that the use of antibacterials from natural ingredients has begun to be used. Star fruit (Averrhoa bilimbi L.) contains flavonoids, tannins, saponins which are useful as antibacterials. Betel leaf (Piper betle) contains saponins, tannins, flavonoids and phenolic compounds which have activity in inhibiting bacteria. The purpose of this study was to determine the antibacterial activity of a combination of star fruit and betel leaf extracts. The method used in this study was the hydroextraction method with various concentrations of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% and 100%. Antibacterial activity testing using disc diffusion. The results showed that the combination of star fruit and betel leaf extracts had optimum inhibitory activity at a concentration of 100% with an inhibition diameter of 11.05 mm for Staphylococcus aureus which was strong and with a diameter of 9.17 mm for Escherichia coli which was moderate.

Uji Efektivitas Antioksidan Serum Ekstrak Daun Randu (Ceiba Pentandra (L.) Gaertn.) Dengan Metode DPPH Muadifah, Afidatul; Ummah, Ratih Salimil; Tilarso, Dara Pranidya
Jurnal Farmasi & Sains Indonesia Vol 6 No 2 (2023)
Publisher : LPPM Sekolah Tinggi Ilmu Farmasi Nusaputera

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52216/jfsi.vol6no2p43-54

The development of cosmetics from natural ingredients has been widely applied in several cosmetic industries, one of the developing cosmetic dosage forms is serum. One of the plants that can be used for serum is randu leaf (Ceiba pentandra (L.) Gaertn.) which contains secondary metabolites including flavonoids, alkaloids, saponins, and tannins which act as natural antioxidants for antiaging. This study aims to determine the optimum levels of flavonoids in randu leaf extract using a UV-Vis spectrophotometer, physical quality of preparations and antioxidant activity in serum preparations using the DPPH method. In determining the levels of flavonoids in the randu leaf extract with concentration series of 20 ppm, 40 ppm, 60 ppm using a UV-Vis spectrophotometer, the results obtained were the optimum concentration of cotton leaf extract in the 60 ppm concentration series, which was 229.3 µg/ml. Then the serum preparation was made, it was found that the serum preparations of randu leaf extract and vitamin C had fulfilled the physical quality test requirements. The antioxidant activity test of serum preparations was continued using the DPPH method using a UV-Vis spectrophotometer and ascorbic acid as a comparison. The ????????50 value for the randu leaf extract serum preparation was 75.0159 ppm which was classified as having strong antioxidant activity while the vitamin C serum preparation was 4.674 ppm which was classified as having very strong antioxidant activity

Pengaruh Suhu Pemanasan dan Variasi Konsentrasi Kombinasi Ekstrak Buah Belimbing Wuluh (Averrhoa bilimbi L.) dan Daun Sirih (Piper betle) Terhadap Bakteri Staphylococcus Aureus dan Escherichia Coli Tilarso, Dara; Muadifah, Afidatul; Indah Pratiwi, Putri; Hariani Nurjanah, Mutia
FASKES : Jurnal Farmasi, Kesehatan, dan Sains Vol. 2 No. 1 (2024): Bulan Juli 2024 Faskes : Jurnal Farmasi, Kesehatan, dan Sains
Publisher : Program Studi Farmasi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32665/faskes.v2i1.3047

Latar belakang Buah belimbing dan daun sirih telah terbukti berkhasiat sebagai antibakteri yang dapat menghambat bakteri Staphylococcus aureus dan Escherichia coli karena mengandung senyawa sebagai antibakteri yaitu flavonoid, tanin, alkaloid, terpenoid, dan saponin. Tujuan: Mengetahui pengaruh suhu pemanasan dan variasi konsentrasi terhadap aktivitas antibakteri kombinasi ekstrak buah belimbing wuluh dan daun sirih menggunakan perbandingan 1:2 terhadap bakteri Staphylococcus aureus dan Escherichia coli. Metode: Metode hidroekstraksi yang digunakan dengan variasi suhu 40°C, 50°C, 60°C, dan 90°C serta dengan variasi konsentrasi 10%, 50%, dan 100%, serta difusi cakram untuk uji aktivitas bakteri. Hasil: Kombinasi ekstrak buah belimbing wuluh dan daun sirih memiliki aktivitas daya hambat optimum pada suhu 50°C pada konsentrasi 100% dengan diameter hambat Staphylococcus aureus sebesar 25,5 mm yang bersifat sangat kuat dan dengan diameter hambat Escherichia coli sebesar 22,17 mm yang bersifat kuat. Simpulan dan saran: Kombinasi ekstrak buah belimbing dan daun sirih memiliki potensi sebagai antibakteri. Diperlukan uji lanjutan untuk mengetahui senyawa spesifik yang terkandung dalam kombinasi ekstrak.

Pengaruh Metode Maserasi dan Soxhletasi Terhadap Kandungan Senyawa Penangkap Radikal Bebas Daun Kersen Menggunakan LC-MS Muadifah, Afidatul; Putri, Amalia Eka; Rahmawati, Desti Linda Dwi; Yudhantara, Sandi Mahesa
Majalah Farmaseutik Vol 21, No 2 (2025)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/farmaseutik.v21i2.99625

Daun Kersen (Muntingia Calabura L.) mempunyai senyawa metabolit sekunder seperti fenol, flavonoid, terpenoid, tanin, saponin dan alkaloid yang berpotensi menjadi penangkap radikal bebas alami. Penelitian ini bertujuan untuk membandingkan kandungan senyawa penangkap radikal bebas di daun kersen (Muntingia Calabura L.) yang diekstraksi secara maserasi dan soxhletasi. Analisis senyawa pada daun kersen (Muntingia Calabura L.) menggunakan Liquid Chromatograph Mass Spectrometry (LCMS). Hasil analisis LCMS ekstrak maserasi daun kersen (Muntingia Calabura L.) yaitu terdapat 85 senyawa terdiri dari flavonoid 68,63%, fenol 11,67%, terpenoid 2,77%, alkaloid 0,3%, saponin 2,2%, dan tanin 1,3%. Pada ekstrak soxhletasi terdapat 92 senyawa terdiri dari flavonoid 71,47%, fenol 10,16%, terpenoid 23,91%, alkaloid 1,04%, tanin 1,4%, dan saponin 1,5%. Analisis senyawa LCMS juga diperkuat dengan uji aktivitas penangkapan radikal bebas menggunakan DPPH pada ekstrak soxhletasi nilai 69,428 ppm sedangkan ekstrak maserasi 74,407 ppm, kedua ekstrak dikategorikan penangkap radikal bebas kuat. Dengan demikian, kandungan senyawa penangkap radikal bebas hasil analisis dengan LC-MS pada daun kersen (Muntingia Calabura L.) dari metode ekstraksi soxhletasi memperoleh persentase (% kadar) yang lebih tinggi dibandingkan dengan ekstraksi maserasi, didukung dengan uji DPPH ekstraksi soxhletasi dapat menghasilkan nilai lebih kecil dari pada metode maserasi.

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