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Pooled Serum in House sebagai Bahan Kontrol untuk Pemantapan Mutu Internal Nuraeni, Hanny Siti; Armal, Hadits Lissentiya; Astriani, Ranti Dwi
Journal of Medical Laboratory Research Vol. 3 No. 1 (2025): Journal of Medical Laboratory Research
Publisher : Politeknik Kesehatan Kementerian Kesehatan Banten

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36743/jomlr.v3i1.827

Abstract

One method used to carry out internal quality control at the analytical stage is to use control serum. . For some clinical laboratories, especially primary clinical laboratories where the number of clinical examinations is still small, the use of commercial control serum for quality control is not affordable because the cost is very expensive. Production of control materials from pooled blood serum can be used as an alternative to expensive production controls. The aim of this study was to find out whether serum collected at home can be used as a control material to carry out quality tests by determining its homogeneity and stability.The implementation process includes taking samples, sample from 48 person and then making pooled serum, examining pooled serum, homogeneity and stability tests, determining reference value ranges, and analyzing results. The combined serum that has been made is homogeneous and stable in the parameters of glucose and total cholesterol, while it is unstable in uric acid. The pooled serum value range for glucose is 49-86 mg/dL, total cholesterol 196-288 mg/dL and uric acid 1.36-3.70 mg/dL. The results of making pooled serum as a control material are declared homogeneous and stable in terms of glucose and total cholesterol parameters so that it can be used as quality control in daily activities. Suggestions for future researchers to add other parameters related to clinical chemistry examinations.
Stabilitas Bahan Kontrol Buatan Sendiri Untuk Pemeriksaan Hemoglobin Astriani, Ranti Dwi; Nuraeni, Hanny Siti; Barlian; Hamtini
Journal of Medical Laboratory Research Vol. 3 No. 1 (2025): Journal of Medical Laboratory Research
Publisher : Politeknik Kesehatan Kementerian Kesehatan Banten

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36743/jomlr.v3i1.828

Abstract

Pemantapan Mutu Laboratorium bertujuan menjamin ketelitian dan ketepatan hasil pemeriksaan laboratorium. Di bidang hematologi, pemeriksaan hemoglobin merupakan beban kerja utama, maka diperlukan suatu bahan kontrol yang selalu tersedia. Salah satu aspek pemantapan kualitas laboratorium adalah penggunaaan bahan kontrol sebagai pemantauan kinerja pemeriksaan. Di negara berkembang, ketidaktersediaan dan harga bahan kontrol komersial menjadi masalah tersendiri. Bahan kontrol buatan sendiri dapat menjadi alternatif untuk memangkas biaya kendali mutu pemerikasaan laboratorium. Bahan kontrol buatan sendiri berasal dari 2 kantong darah yang dibuat menjadi hemolisat dengan kadar hemoglobin rendah, normal, dan tinggi, kemudian masing-masing ditentukan kadarnya menggunakan rerata ± standar deviasi. Selanjutnya, dilakukan uji homogenitas dan uji stabilitas untuk mengetahui apakah hemolisat (kontrol Hb) yang telah dibuat stabil dan homogen sehingga layak dijadikan bahan kontrol Hemoglobin. Pada bahan kontrol buatan sendiri kadar Hb rendah yaitu 5,4-5,6 g/dL, Hb normal 12,48-12,60, dan Hb tinggi 16,27-16,40 sedangkan pada kontrol Hb yang berasal dari pabrikan, kadar Hb rendah 5,2-5,8 g/dL, Hb normal 12,2-13,2 g/dL, dan Hb tinggi 15,9-17,3 g/dL. Uji homogenitas dan stabilitas menunjukan bahwa baik hemolisat maupun pabrikan memberikan hasil uji homogen dan stabil. Perbedaan bermakna terdapat pada nilai homogenitas dan stabilitas Hb rendah antara Hemolisat dan pabrik (Sig.000) sedangkan nilai homogenitas dan stabilitas Hb normal dan tinggi pada hemolisat dan pabrik tidak terdapat perbedaan yang bermakna. Bahan kontrol buatan sendiri dengan kadar Hb rendah, normal, dan tinggi didapatkan homogen dan stabil. Uji t independen menunjukan adanya perbedaan bermakna kadar Hb rendah antara hemolisat dan pabrikan.
PENENTUAN NILAI RUJUKAN LAJU ENDAP DARAH PADA WANITA USIA 20-50 TAHUN Nuraeni, Hanny Siti; Astriani, Ranti Dwi; Shufiyani, Shufiyani; Destriana, Destriana
jitek Vol 11 No 1 (2023): September 2023
Publisher : Poltekkes Kemenkes Jakarta III

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32668/jitek.v11i1.1113

Abstract

ESR (Erythrocyte Sedimentation Rate) is one of the parameters used for health checks. The ESR value is used to determine the presence of inflammation and infection and monitor disease. The reference value is an examination used to see a normal rather than a laboratory examination of a patient. The problem is that the current ESR reference values ​​refer to lab parameter insert kits and WHO, where reference values ​​may vary between geographic regions, age groups, gender and race. Based on the International Council for Standardization in Hematology (ICSH) and ISO 15189 Clause 5.2.2, the range of reference values ​​must be stated based on the local area minimum of 120 samples. The population used in this research was Periuk sub-district, Tangerang City, is 191 and the sample was 126 people with a random sample. Determining the reference value range in this study used primary data using statistical calculations based on CLSI EP28-A3C guidelines, namely the 2.5 and 97.5 percentiles. The research results obtained a degree of ESR reference values ​​of 0-39 mm/hour. Suggestions for further research are to determine reference values ​​based on gender and racial distance.
Preeclampsia support group sebagai upaya pemberdayaan kader dalam pencegahan dan pengendalian preeklampsia Patricia, Venny; Yani, Ahmad; Nuraeni, Hanny Siti; Astriani, Ranti Dwi; Rumiatun, Darti
SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Vol 8, No 1 (2024): March
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jpmb.v8i1.22228

Abstract

AbstrakPreeklampsia merupakan salah satu penyumbang utama angka kematian ibu di Indonesia. Salah satu upaya menurunkan preeklampsia adalah melalui penyuluhan terhadap kader mengenai preeklampsia, deteksi dini, serta pencegahannya. Kader merupakan perpanjangan tangan dari tenaga kesehatan di tingkat desa sehingga dapat diberdayakan dalam upaya pencegahan dan pengendalian preeklampsia, terutama dalam memberikan pemahaman kepada ibu hamil di wilayah masing-masing. Dalam pengabdian ini, dilakukan penyuluhan dan pendampingan mengenai deteksi dini preeklampsia kepada para kader. Sasaran kegiatan ini adalah para kader di 14 desa di kecamatan Padarincang, kabupaten Serang, Banten. Kegiatan ini bertujuan meningkatkan pengetahuan dan keterampilan kader mengenai pencegahan dan pengendalian preeklampsia serta deteksi dini preeklampsia pada ibu hamil melalui pemeriksaan tekanan darah dan protein urine melalui pemanfaatan support group. Kegiatan dilakukan dalam 4 tahapan yaitu pengurusan perizinan, penyuluhan, skrining preeklampsia, serta monitoring dan evaluasi keberlanjutan program. Hasil pengabdian menunjukkan bahwa pengetahuan dan keterampilan kader mengenai preeklampsia, deteksi dini, dan upaya pencegahannya mengalami peningkatan sesudah diberi penyuluhan. Pengetahuan dan keterampilan kader dengan kategori baik yaitu sebesar 15% sebelum diberikan penyuluhan dan meningkat menjadi sebesar 55% setelah diberikan penyuluhan. Support group yang dibentuk terbukti efektif dalam membangun komunikasi dan koordinasi dalam upaya pencegahan dan pengendalian preeklampsia di wilayah kecamatan Padarincang. Untuk menjamin keberlanjutan program, preeclampsia support group tetap digunakan sebagai sarana koordinasi antara para kader, bidan desa, tenaga kesehatan lainnya di puskesmas, aparatur desa dan kecamatan, serta tim pengabdian. Kata kunci: bidan; hipertensi; kader; preeklampsia; proteinuria. AbstractPreeclampsia is one of the main contributors to maternal mortality rates in Indonesia. One effort to reduce preeclampsia is through educating cadres since they are an extension of health workers so that they can be empowered in efforts to prevent and control preeclampsia, especially in providing understanding to pregnant women in their areas. In this community service, education and assistance regarding the early detection of preeclampsia were provided to cadres. The target was cadres in 14 villages in Padarincang, Serang district, Banten. This activity aimed to increase cadres' knowledge and skills regarding the prevention and control of preeclampsia as well as the early detection of preeclampsia in pregnant women through checking blood pressure and urine protein through the use of support groups. Activities were carried out in 4 stages, namely processing activity permits, counseling, preeclampsia screening, and monitoring and evaluating program sustainability. The results of the service showed that the cadres' knowledge and skills regarding preeclampsia, early detection, and prevention efforts had increased after being given counseling. The knowledge and skills of cadres in the good category were 15% before being given counseling and increased to 55% after being given counseling. The support group was proven to be effective in building communication and coordination in efforts to prevent and control preeclampsia in the Padarincang sub-district area. To ensure the continuation of the program, the support group is still used as a means of coordination between cadres, midwives, community health center authorities, village and sub-district officials, and the community service team. Keywords: midwife; hypertension; cadre; preeclampsia; proteinuria.
Pemanfaatan Ekstrak Bunga Telang (Clitoria Ternatea L.) Sebagai Alternatif Zat Warna Lpcb Pada Pewarnaan Fungi hamtini, Hamtini; Hakim, Syamsir; Astriani, Ranti Dwi; Fadillah, Muhammad Arief; Rahmawati, Juli
Journal of Medical Laboratory Research Vol. 2 No. 3 (2024): Journal of Medical Laboratory Research
Publisher : Politeknik Kesehatan Kementerian Kesehatan Banten

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The eagle flower is a compound flower that is identical to the purple color on the petals. Vines, one of which is eagle flowers, can be found anywhere from the yard of the house to the edge of the rice field. The traditional use for this plant is as an eye medicine and food coloring, and it can also be grown as an ornamental plant. There are two types of dyes, namely dyes made from chemicals and dyes made from plants. Most of the dyes used in laboratories are generally chemicals such as KOH, methylene blue, and lactofenol, which are not good for the environment and are quite expensive. This means that it is necessary to make other dyes that are better for the environment. The purpose of this study was to determine whether or not striped leaf extract can be used as an alternative to LPCB staining in fungi. The method used in this study was maceration of telang flower extract with 96% ethanol solvent, after obtaining the extraction results then dilution was carried out with concentrations of 1%, 2.5%, 5%, 7.5% and 10% using aqueous solvents. The result of this study is that striped flower extract cannot be used as a dye in fungal coloring, this can be seen from the morphological picture of fungi that are not clearly visible and do not absorb dyes from eagle flower extract compared to LPCB dyes used as standard controls.