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All Journal Jurnal Ilmu dan Teknologi Kesehatan Journal of Medical Laboratory Research
Astriani, Ranti Dwi
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Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Health Professions Immunology & microbiology Medicine & Pharmacology

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Pooled Serum in House sebagai Bahan Kontrol untuk Pemantapan Mutu Internal Nuraeni, Hanny Siti; Armal, Hadits Lissentiya; Astriani, Ranti Dwi
Journal of Medical Laboratory Research Vol. 3 No. 2 (2025): Journal of Medical Laboratory Research
Publisher : Politeknik Kesehatan Kementerian Kesehatan Banten

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36743/jomlr.v3i1.827

Abstract

One method used to carry out internal quality control at the analytical stage is to use control serum. . For some clinical laboratories, especially primary clinical laboratories where the number of clinical examinations is still small, the use of commercial control serum for quality control is not affordable because the cost is very expensive. Production of control materials from pooled blood serum can be used as an alternative to expensive production controls. The aim of this study was to find out whether serum collected at home can be used as a control material to carry out quality tests by determining its homogeneity and stability.The implementation process includes taking samples, sample from 48 person and then making pooled serum, examining pooled serum, homogeneity and stability tests, determining reference value ranges, and analyzing results. The combined serum that has been made is homogeneous and stable in the parameters of glucose and total cholesterol, while it is unstable in uric acid. The pooled serum value range for glucose is 49-86 mg/dL, total cholesterol 196-288 mg/dL and uric acid 1.36-3.70 mg/dL. The results of making pooled serum as a control material are declared homogeneous and stable in terms of glucose and total cholesterol parameters so that it can be used as quality control in daily activities. Suggestions for future researchers to add other parameters related to clinical chemistry examinations.
Stabilitas Bahan Kontrol Buatan Sendiri Untuk Pemeriksaan Hemoglobin Astriani, Ranti Dwi; Nuraeni, Hanny Siti; Barlian; Hamtini
Journal of Medical Laboratory Research Vol. 3 No. 2 (2025): Journal of Medical Laboratory Research
Publisher : Politeknik Kesehatan Kementerian Kesehatan Banten

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36743/jomlr.v3i1.828

Abstract

Pemantapan Mutu Laboratorium bertujuan menjamin ketelitian dan ketepatan hasil pemeriksaan laboratorium. Di bidang hematologi, pemeriksaan hemoglobin merupakan beban kerja utama, maka diperlukan suatu bahan kontrol yang selalu tersedia. Salah satu aspek pemantapan kualitas laboratorium adalah penggunaaan bahan kontrol sebagai pemantauan kinerja pemeriksaan. Di negara berkembang, ketidaktersediaan dan harga bahan kontrol komersial menjadi masalah tersendiri. Bahan kontrol buatan sendiri dapat menjadi alternatif untuk memangkas biaya kendali mutu pemerikasaan laboratorium. Bahan kontrol buatan sendiri berasal dari 2 kantong darah yang dibuat menjadi hemolisat dengan kadar hemoglobin rendah, normal, dan tinggi, kemudian masing-masing ditentukan kadarnya menggunakan rerata ± standar deviasi. Selanjutnya, dilakukan uji homogenitas dan uji stabilitas untuk mengetahui apakah hemolisat (kontrol Hb) yang telah dibuat stabil dan homogen sehingga layak dijadikan bahan kontrol Hemoglobin. Pada bahan kontrol buatan sendiri kadar Hb rendah yaitu 5,4-5,6 g/dL, Hb normal 12,48-12,60, dan Hb tinggi 16,27-16,40 sedangkan pada kontrol Hb yang berasal dari pabrikan, kadar Hb rendah 5,2-5,8 g/dL, Hb normal 12,2-13,2 g/dL, dan Hb tinggi 15,9-17,3 g/dL. Uji homogenitas dan stabilitas menunjukan bahwa baik hemolisat maupun pabrikan memberikan hasil uji homogen dan stabil. Perbedaan bermakna terdapat pada nilai homogenitas dan stabilitas Hb rendah antara Hemolisat dan pabrik (Sig.000) sedangkan nilai homogenitas dan stabilitas Hb normal dan tinggi pada hemolisat dan pabrik tidak terdapat perbedaan yang bermakna. Bahan kontrol buatan sendiri dengan kadar Hb rendah, normal, dan tinggi didapatkan homogen dan stabil. Uji t independen menunjukan adanya perbedaan bermakna kadar Hb rendah antara hemolisat dan pabrikan.
PENENTUAN NILAI RUJUKAN LAJU ENDAP DARAH PADA WANITA USIA 20-50 TAHUN Nuraeni, Hanny Siti; Astriani, Ranti Dwi; Shufiyani, Shufiyani; Destriana, Destriana
jitek Vol 11 No 1 (2023): September 2023
Publisher : Poltekkes Kemenkes Jakarta III

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32668/jitek.v11i1.1113

Abstract

ESR (Erythrocyte Sedimentation Rate) is one of the parameters used for health checks. The ESR value is used to determine the presence of inflammation and infection and monitor disease. The reference value is an examination used to see a normal rather than a laboratory examination of a patient. The problem is that the current ESR reference values ​​refer to lab parameter insert kits and WHO, where reference values ​​may vary between geographic regions, age groups, gender and race. Based on the International Council for Standardization in Hematology (ICSH) and ISO 15189 Clause 5.2.2, the range of reference values ​​must be stated based on the local area minimum of 120 samples. The population used in this research was Periuk sub-district, Tangerang City, is 191 and the sample was 126 people with a random sample. Determining the reference value range in this study used primary data using statistical calculations based on CLSI EP28-A3C guidelines, namely the 2.5 and 97.5 percentiles. The research results obtained a degree of ESR reference values ​​of 0-39 mm/hour. Suggestions for further research are to determine reference values ​​based on gender and racial distance.
Pemanfaatan Ekstrak Bunga Telang (Clitoria Ternatea L.) Sebagai Alternatif Zat Warna Lpcb Pada Pewarnaan Fungi hamtini, Hamtini; Hakim, Syamsir; Astriani, Ranti Dwi; Fadillah, Muhammad Arief; Rahmawati, Juli
Journal of Medical Laboratory Research Vol. 3 No. 1 (2024): Journal of Medical Laboratory Research
Publisher : Politeknik Kesehatan Kementerian Kesehatan Banten

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The eagle flower is a compound flower that is identical to the purple color on the petals. Vines, one of which is eagle flowers, can be found anywhere from the yard of the house to the edge of the rice field. The traditional use for this plant is as an eye medicine and food coloring, and it can also be grown as an ornamental plant. There are two types of dyes, namely dyes made from chemicals and dyes made from plants. Most of the dyes used in laboratories are generally chemicals such as KOH, methylene blue, and lactofenol, which are not good for the environment and are quite expensive. This means that it is necessary to make other dyes that are better for the environment. The purpose of this study was to determine whether or not striped leaf extract can be used as an alternative to LPCB staining in fungi. The method used in this study was maceration of telang flower extract with 96% ethanol solvent, after obtaining the extraction results then dilution was carried out with concentrations of 1%, 2.5%, 5%, 7.5% and 10% using aqueous solvents. The result of this study is that striped flower extract cannot be used as a dye in fungal coloring, this can be seen from the morphological picture of fungi that are not clearly visible and do not absorb dyes from eagle flower extract compared to LPCB dyes used as standard controls.
Co-Authors Armal, Hadits Lissentiya Barlian DESTRIANA DESTRIANA, DESTRIANA Fadillah, Muhammad Arief Hakim, Syamsir Hamtini - Hamtini Nuraeni, Hanny Siti Rahmawati, Juli Shufiyani, Shufiyani