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Ketahanan dan Kulturabilitas Cronobacter Sakazakii terhadap Stres Kering pada Simulasi Proses Pengeringan Siti Nurjanah; Ratna Nurmalita Sari; Ratih Dewanti-Hariyadi
Jurnal Mutu Pangan : Indonesian Journal of Food Quality Vol. 4 No. 2 (2017)
Publisher : Department of Food Science and Technology (ITP), Faculty of Agricultural Technology, Bogor Agricultural University (IPB) in collaboration with the Indonesian Food and Beverage Association (GAPMMI), the National Agency of Drug and Food Control, and th

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Abstract

Cronobacter sakazakii is an opportunistic pathogen that has been isolated from several dried food products and reported can survive in dry conditions. Survival on dry stress can be observed using microscopy due to losses of their culturability in the medium. This study aimed to observe the survival and culturability of C. sakazakii on dry stress and ability to recover their culturability or resuscitation. This research was carried out in 3 steps: drying cells by desiccation, survival cell enumeration, and resuscitation. The bacteria used were two isolates Green fluorescence protein (pGFPuv) mutants of C. sakazakii, namely E2 and Yrt2a, that have ampicillin resistance and similar growth pattern with their wild-type. Desiccation was conducted by placed bacterial cells in incubator at 30, 35, 400 and 500C for 2 hours and air-drying stored at 210C for 72 hours. The culture able cells were enumerated on tryptone soy agar (TSA) and total cells include non-culturable cells were enumerated using fluorescence microscopy. Both of C. sakazakii isolates can survive for all of treatment. Total cells of E2 and Yrt2a decreased 2-3 and 4-5 log cells respectively. Both of isolates loss their ability to grow on medium, however suplementation the medium growth using pyruvate can resuscitate their cells and recover their culturability. Resuscitation of C. sakazakii cells might be as potential risk for increasing bacterial contaminant in food.
PENILAIAN ASUPAN GIZI DAN TINGKAT KECUKUPAN GIZI IBU HAMIL MENGGUNAKAN METODE 24 HOUR RECALL DI KECAMATAN MOYO HULU , SUMBAWA BESAR, NUSA TENGGARA BARAT Lukman Azis; Ratna Mandasari; Ratna Nurmalita Sari
Jurnal Teknologi Pangan dan Kesehatan (The Journal of Food Technology and Health) Vol 4, No 2 (2022): November
Publisher : Universitas Sahid

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36441/jtepakes.v4i2.1325

Abstract

ABSTRAK: Kekurangan gizi merupakan permasalahan kesehatan global yang terjadi hampir 80% negara di dunia. Ibu hamil merupakan salah satu kelompok populasi yang rentan mengalami kekurangan gizi. Dampak buruk kekurangan gizi tidak hanya pada kesehatan ibu namun juga janinnya. Tujuan penelitian ini adalah untuk menilai asupan gizi ibu hamil di kecamatan Moyo Hulu kabupaten Sumbawa Besar, Nusa Tenggara Barat. Sebuah metode cross sectional melibatkan 15 ibu hamil berusia 30 tahun dengan usia kehamilan 27 minggu. Metode 24 hour recall digunakan untuk mencatat jenis dan kuantitas konsumsi makanan ibu hamil selama 3 hari secara tidak berututan, yaitu dua hari aktif dan satu hari di akhir pekan. Data hasil recall dianalisa menggunakan software Nutrisurvey. Hasil penelitian ini menunjukkan bahwa rerata asupan energi ibu hamil adalah 2,979 Kkal dan asupan zat gizi makro seperti karbohidrat, protein dan lemak adalah 368 g/hari, 142 g/hari dan 99 g/hari. Hampir seluruh asupan zat gizi mikro ibu hamil telah melebihi Angka Kecukupan Gizi (AKG) namun asupan zat gizi mikro seperti zat besi dan folat masih dibawah AKG yaitu 17 mg/hari dan 286 µg/hari, secara berurutan. Konsumsi serat pangan diketahui juga kurang dari AKG yaitu 15 g/hari. Sementara itu, kelompok makanan yang paling tinggi dikonsumsi ibu hamil adalah bahan pokok berpati seperti nasi (893 g/hari) dan yang paling rendah dikonsumsi adalah buah dan sayur kaya vitamin A (48 g/hari). Kesimpulan penelitian ini adalah ibu hamil telah memenuhi asupan zat gizi makro dan mikro meskipun asupan zat besi, folat dan serat pangan masih belum memenuhi rekomendasi AKG karena asupan buah dan sayur yang rendah. ABSTRACT: Malnutrition is a global health problem that occurs almost 80% countries in the world. Pregnant women are one of the population groups that are vulnerable to nutritional deficiencies. The effects of nutritional deficiencies not only affect the mothers’ health but also the fetus. The aim of this study was to assess the nutritional intake of pregnant women in Moyo Hulu sub-district, Sumbawa Besar district, West Nusa Tenggara. A cross sectional method involved 15 pregnant women aged 30 years with a gestational age of 27 weeks. The 24-hour recall method was used to record the type and quantity of foods consumed by pregnant women for 3 non-consecutive days; two weekdays and one weekend. The recall data were analyzed using Nutrisurvey software. The results of this study indicated that the average energy intake of pregnant women was 2,979 Kcal and the intake of macronutrients such as carbohydrates, protein and fat were 368 g/day, 142 g/day and 99 g/day. Almost all micronutrient intakes of pregnant women had exceeded the Angka Kecukupan Gizi (AKG), but the intake of macronutrients such as iron and folate were still below the AKG of 17 mg/day and 286 g/day, respectively. Consumption of dietary fiber was also known to be less than the AKG, which was 15 g/day. Meanwhile, the highest food group consumed by pregnant women was starchy staples e.g. rice (893 g/day) and the lowest consumed were vitamin A-rich fruits and vegetables (48 g/day). The conclusion of this study was that pregnant women had fulfilled their macro- and micronutrient even though  iron, folate and dietary fiber did not meet the AKG recommendation due to low intake of  fruits and vegetables.
PENDAMPINGAN PENGOLAHAN DAN PENGEMASAN DENDENG DAGING KERBAU FERMENTASI DI KELOMPOK PEMBERDAYAAN DAN KESEJAHTERAAN KELUARGA DESA BATU BULAN KECAMATAN MOYO HULU Ihlana Nairfana; Chairul Anam Afgani; Ratna Nurmalita Sari; Veni Rori Setiawati
Jurnal Abdi Insani Vol 9 No 3 (2022): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v9i3.677

Abstract

Local residents of Batu Bulan Village have been processing buffalo meat into traditional flavored jerky called “raret”. The jerky is marinated in salt and tamarin juice therefore has a less varied taste. Besides that, the long drying time of the jerky makes the texture become tough. Therefore ready-to-eat jerky is one of the innovations to make beef jerky easier to process and maintain its nutritional content. Fermentation lactic acid bacteria (BAL) is one well-known processing technique that are considered most suitable. The aim of this program is to increase the added value of buffalo jerky through processing and packaging innovation. The method used is socialization, counseling, as well as training and mentoring. Furthermore, the implementation of the socialization event took place at the Batu Bulan Village Office which was attended by the woman empowerment and village officials. The results obtained from this activity are the increased ability of Batu Bulan woman empowerment in processing and packaging ready-to-eat buffalo jerky. There has been an increase in the ability of Batu Bulan women in developing their business from the aspect of producing and product packaging.
PENILAIAN STATUS GIZI IBU MENYUSUI EKSKLUSIF DENGAN METODE 24 H RECALL DI KECAMATAN SUMBAWA Ratna Nurmalita Sari; Murniyati; Lukman Azis
Jurnal TAMBORA Vol. 7 No. 2 (2023): EDISI 19
Publisher : Wakil Rektor 3, Direktorat Riset, Publikasi dan Inovasi, Universitas Teknologi Sumbawa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36761/jt.v7i2.2968

Abstract

Asupan gizi yang cukup merupakan faktor penting dalam pemenuhan kebutuhan ibu menyusui dan bayinya, terutama pada periode ASI eksklusif. Kekurangan gizi dapat berdampak pada kualitas air susu ibu (ASI) yang dihasilkan dan meningkatkan risiko stunting. Penelitian ini bertujuan untuk mengetahui status gizi ibu menyusui pada periode eksklusif menggunakan metode 24h recall di Kecamatan Sumbawa. Studi ini dilakukan menggunakan metode cross-sectional yang dilakukan pada 24 ibu menyusui. Asupan ibu menyusui juga dikelompokan dengan menggunakan pedoman Food Agriculture Organization (FAO), sementara asupan gizi dinilai menggunakan Nutrisurvey. Hasil penelitian menunjukkan bahwa asupan gizi makro dan mikro melebihi AKG. Namun, terdapat 6 zat gizi mikro yang berada di bawah AKG yaitu asam folat, vitamin E, vitamin C, kalsium, dan serat. Berdasarkan kelompok makanan yang dikonsumsi, kelompok makanan berpati merupakan kelompok paling banyak dikonsumsi oleh responden.
PENILAIAN DIMENSI TUBUH SEBAGAI SALAH SATU PREDIKSI KEKURANGAN NUTRISI PADA IBU MENYUSUI DAN BAYINYA DI KECAMATAN MOYO HULU Ratna Nurmalita Sari; Dwi Aprilian Nur Putri; Lukman Azis
The Journal of Teknologi Pangan Vol 3 No 2 (2022): Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering
Publisher : Faculty of Agricultural Technology

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Abstract

Menyusui merupakan salah satu cara pemberian makan secara langsung dari ibu ke bayi. Bayi yang baru dilahirkan direkomendasikan untuk disusui secara eksklusif minimal enam bulan sampai dengan dua tahun. Kerentanan nutrisi terhadap ibu menyusui dan bayi berdampak pada kekurangan nutrisi seperti kekurangan magnesium, vitamin B6, folat, kalsium, dan seng. Prediksi stunting dapat dilakukan dengan pengukuran dimensi tubuh bayi seperti lingkar kepala, tinggi badan (TB) dan berat badan (BB). Penelitian ini bertujuan untuk mengetahui dimensi tubuh sebagai salah satu metode prediksi kekurangan nutrisi pada ibu menyusui dan bayinya di Kecamatan Moyo Hulu, Kabupaten Sumbawa. Penelitian ini menggunakan metode cross sectional study yang dilakukan pada 15 ibu menyusui dan bayinya. Penilaian dilakukan dengan menggunakan Aplikasi WHO Anthro. Hasil dari penelitian menunjukkan ibu menyusui di Kecamatan Moyo Hulu memiliki rerata IMT 21,95 %. Hal ini menunjukkan bahwa ibu menyusui di Kecamatan Moyo Hulu memiliki IMT yang normal. Bayi diukur dalam 3 kategori antropometri yakni BMI-for-Age-Z score (BAZ), Height-for-Age-Z score (HAZ), dan Weight-for-Age-Z score (WAZ). BAZ pada bayi di Kecamatan Moyo Hulu terdapat 2 kategori, yakni obesitas dengan rerata 26,67%, dan normal dengan rerata 73,33%. HAZ pada bayi ada 2 kategori juga, yakni kategori normal dengan rerata 53,33% dan kerdil sampai sangat kerdil dengan rerata 46,67%. WAZ pada bayi ada 3 kategori, yakni normal dengan rerata 86,67%, kurus dengan rerata 6,67%, dan sangat kurus dengan rerata 6,67%.
The change of bile salt stimulated lipase during 6 months and the correlation with macronutrients in Chinese human milk Ratna Nurmalita Sari; Lu Jing; Lv Jiaping
jurnal1 VOLUME 6 ISSUE 2, DECEMBER 2023
Publisher : Hasanuddin University Food Science and Technology Study Program

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/canrea.v6i2.1033

Abstract

Bile salt stimulated lipase (BSSL) is the most abundant lipase in human milk which plays a pivotal role in new-born fat digestion, especially in the first six months of life. Lipid as the main energy supply for infant was very depending on BSSL activity for obtaining optimal lipid absorption under immature digestion track and pancreatic system condition. Due to the important role of BSSL, this research was addressed to study the effect of gender and lactation stage on BSSL activity and the correlation of BSSL with macronutrients content in Chinese human milk. Cross sectional and longitudinal study were used to investigate the BSSL activity and concentration pattern during 6 months of lactation. A declining pattern of both BSSL activity and concentration was observed as lactation stage progressed. The BSSL concentration significantly declined from 138.56 to 97.07 μg/ml during 6 months. Significant differences of BSSL activity and concentration were also observed in human milk for different gender babies. Male babies had breastmilk with less BSSL rather than female babies. Protein had strong correlation with BSSL activity and concentration, while carbohydrate had non-significant negative coefficient correlation. However, no correlation observed between fat content and BSSL.
PENILAIAN DIMENSI TUBUH SEBAGAI SALAH SATU PREDIKSI KEKURANGAN NUTRISI PADA IBU MENYUSUI DAN BAYINYA DI KECAMATAN MOYO HULU Ratna Nurmalita Sari; Dwi Aprilian Nur Putri; Lukman Azis
The Journal of Teknologi Pangan Vol 3 No 2 (2022): Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering
Publisher : Faculty of Agriculture Science & Technology

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Abstract

Breastfeeding is recommended way of feeding directly from mother to baby. Newborns are recommended to be exclusively breastfed for at least six months to two years. Nutritional vulnerability to breastfeeding mothers and infants has an impact on nutritional deficiencies such as lack of magnesium, vitamin B6, folate, calcium, and zinc. Prediction of stunting can be conducted by measuring the baby body dimensions such as head circumference, height, and body weight. This study aimed to determine body dimensions as a method of predicting nutritional deficiencies in breastfeeding mothers and their babies in Moyo Hulu District, Sumbawa. This study used a cross sectional study method which carried on 15 breastfeeding mothers and their babies. The assessment was carried out using the WHO Anthro apps. The results showed that breastfeeding mothers in Moyo Hulu District had an average BMI of 21.95%. This showed that breastfeeding mothers in Moyo Hulu District have a normal BMI. Therefore, the baby was included in 3 anthropometric categories, including BMI-for-Age-Z score (BAZ), Height-for-Age-Z score (HAZ), and Weight-for-Age-Z score (WAZ). There were 2 categories of BAZ in infants in Moyo Hulu District, namely obesity with an average of 26.67%, and normal with an average of 73.33%. There were also 2 categories of HAZ in infants, including the normal category with an average of 53.33% and stunted to very stunted with an average of 46.67%. There were 3 categories of WAZ in infants, namely normal with an average of 86.67%, thin with an average of 6.67%, and very thin with an average of 6.67%.
BIMBINGAN DAN PELATIHAN PEMBUATAN KOMBUCHA DARI BUAH NANAS DI DESA BATU BULAN KECAMATAN MOYO HULU KABUPATEN SUMBAWA Afgani, Chairul Anam; Sari, Ratna Nurmalita; Nairfana, Ihlana; Setiawati, Veni Rori
Jurnal Agro Dedikasi Masyarakat (JADM) Vol 3, No 2 (2022)
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jadm.v3i2.10041

Abstract

Pinneaple is one of the major commodity in West Nusa Tenggara. This commodity could be processed into commercial product such as kombucha. Kombucha is fermented tea by using Simbiotic Culture of Bacteria and Yeast (SCOBY) which incubated for 14 days. During kombucha processing, fruit juice were diluted then added by sugar which involved as main microorganism nutrition in fermentation. This community development aimed to transfer the knowledge of kombucha making from pine and other domestic fruits. Product processing explanation, disscussion, and practice were conducted to transfer the complete knowledge for the target. The result showed that Batu Bulan community-as the target, could understand the kombucha making and figure its benefit out. 
Gluten-free cookies formulation using local composite flour of gadung, brown rice, and moringa leaves Dayani, Fitri; Sari, Ratna Nurmalita
Journal of Agritechnology and Food Processing Vol 3, No 1 (2023)
Publisher : UNIVERSITAS MUHAMMADIYAH MATARAM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jafp.v3i1.15723

Abstract

Fibre-rich snacks can be made from local ingredients that are abundant and gluten-free, such as gadung and moringa leaves. This study aims to determine the correct formulation in the production of cookies using a combination of local composite flour from gadung tubers, brown rice and moringa leaves. The parameters observed include organoleptic test, fibre content and physical properties of the cookies. This research was carried out using an experimental method with a fully randomised design with 4 treatments and 3 replicates. The composites used consisted of umbi gadung flour, brown rice flour and moringa leaf flour with the formulation 60 g: 30 g: 10 g (P1), 50 g: 20 g: 30 g (P2), 40 g: 40 g: 20 g (P3) and (3 g: 25 g: 40 g (P4). Based on the results of the data analysis using the SPPS programme, it is known that the composite formulation has a significant effect on the organoleptic and physico-chemical parameters of the cookies. The more moringa leaves are added, the lower the panelists' liking, the darker the colour of the biscuits and the more brittle the texture. In conclusion, P1 is the best composite formulation based on panelist preference with yellowish-brown cookie colour, low breakage and crude fibre content of 3.51%.
Microencapsulation technology for lipase added infant formula to improve gastrointestinal digestion: a review Sari, Ratna Nurmalita
Journal of Agritechnology and Food Processing Vol 4, No 2 (2024)
Publisher : UNIVERSITAS MUHAMMADIYAH MATARAM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jafp.v4i2.27980

Abstract

Lipase role in milk lipid digestion is crucial since fat is the common source of infant nutrition. Human milk as the golden standard of infant nutrition contains bile salt-stimulated lipase which plays a significant role in gaining baby’s weight. In achieving the goal of infant formula to mimic human milk, researchers are concerned to add lipase in infant formula called LAIF (lipase-added infant formula). Since lipase is heat labile, microencapsulation is needed as an approach to stabilize lipase by adding in infant formula. This article reviews the possibility of micro-encapsulation for optimizing the lipase addition. Alginate beads encapsulate product from algae which can be used since it is usually used in commercial powder infant formula. Milk proteins also an option since the proteins contain high nutritional values and are categorized as safe materials. In the legumes group, pea protein could be the prospective option since the material has good solubility in water, stability in high temperatures, and good foaming capacity. Microencapsulation technology makes it possible to make longer shef life and improving stability of lipase in infant formula.