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Virulence Characteristic of Avian Pathogenic Escherichia coli (APEC) Isolates Adrenalin, Sruti Listra; Imanjati, Lynda Nugrahaning; Fauziah, Ima; Prakasita3, Vinsa Cantya; Widyarini, Sitarina; Wahyuni, Agnesia Endang Trihastuti
Jurnal Sain Veteriner Vol 38, No 1 (2020): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Avian pathogenic Escherichia coli (APEC) merupakan penyebab kolibasilosis pada unggas, salah satu penyakit pernapasan yang menyebabkan permasalahan yang serius di industri perunggasan. APEC dapat mengakibatkan tingginya jumlah kematian dan angka afkir, penurunan produksi, dan tingginya biaya pengobatan. Manifestasi kolibasilosis yang biasa terjadi adalah airsacculitis, perihepatitis, dan perikarditis. Serotipe APEC yang banyak diidentifikasi di lapangan adalah O1K1, O2K1, dan O78K80. Embryo lethality assay (ELA) adalah salah satu metode yang dapat digunakan untuk mengetahui virulensi serotipe APEC. Tujuan dari penelitian ini adalah untuk mengetahui karakteristik virulensi isolat APEC berbagai serotipe. Lima isolat APEC serotipe O1K1, O2K1, O78K80, O157H7, dan unknown serotype digunakan untuk pengujian virulensi dengan menginokulasikan bakteri ke dalam cairan alantois telur ayam berembrio spesific pathogenic free (TAB SPF) umur 12 hari. Masingmasing serotipe dibutuhkan telur sebanyak 10_butir, dengan dosis bakteri 100-500 CFU/ 0.1 ml. Candling dilakukan setiap hari, sampai umur embrio 18 hari, untuk menentukan jumlah kematian dan perubahan lesi patologi. Setelah diamati selama 6 hari, persentase TAB yang mati setelah diinokulasi dengan APEC serotipe O1K1, O2K1, unknown serotype adalah 100% (10/10), serotipe O78K80 90% (9/10), dan serotipe O157H7 70% (70%). Seluruh ayam yang mati mengalami lesi patologi adanya hemoragi daerah kranial dan ektremitas. Seluruh isolat E. coli dalam penelitian ini memiliki virulensi tinggi.Â

Analysis of Slaughtering Points Distribution during Eid al-Adha 1440H in Malang City, East Java, Indonesia Adrenalin, Sruti Listra; Airlangga, Gegana Wimaldy; Hardian, Andreas Bandang
Veterinary Biomedical and Clinical Journal Vol. 2 No. 2 (2020)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2020.002.02.5

Eid al-Adha is an annual Muslim celebration throughout the world indicated by sacrificing animals such as cattles, goats or sheep. This study aimed to analyse the distribution of slaughtering points, types and the number of animals being slaughtered as well as antemortem and postmortem abnormalities of the carcasses during Eid al-Adha 1440 H in Malang City. Data were collected from the geocode service location and the secondary data were obtained through questionnaire. This study listed a total of 145 slaughtering points located in Blimbing, Kedung Kandang, Klojen, Lowokwaru, and Sukun District with 3102 animals being sacrificed. The slaughtering points were taken place in the office yards, mosque yards, home yards, school yards or other public fields. As many as 75.86% of slaughtering points provided special shelters for the animals before being slaughtered. The antemortem examination resulted that animals from 21.38% of the slaughtering points suffered from conjunctivitis, lesions on the skin, broken horns, and lameness. Proportions of apparent postmortem abnormalities were measured respectively: cattle 64.83%, goat 41.38%, and sheep 4.14%.Â Hence, we recommend to further involve the veterinarians to monitor the animal condition during Eid al-Adha and to ensure the slaughtering processes were done properly.

Management of Avian Influenza H9N2 Vaccination in Broiler Chickens at JAPFA COMFEED Myanmar Pte Ltd. Verian, Bagas Ridjalu; Prasetyo, Dodik; Adrenalin, Sruti Listra; Wuragil, Dyah Kinasih; Cahyo, Budi Nur
Veterinary Biomedical and Clinical Journal Vol. 6 No. 1 (2024)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2024.006.01.3

Avian influenza virus subtype H9N2 (AIV-H9N2) has been a concern for poultry health in the last 20 years. The virus emerged in domestic poultry in the mid-1990s and has been reported in several Asian countries, including Myanmar. AI subtype H9N2 caused huge economic losses to the poultry industry in the form of respiratory distress and 25% weight loss in broiler chickens. This study discusses the administration of Avian Influenza (AI) H9N2 strain inactivated vaccine to commercial broiler chickens at JAPFA COMFEED Myanmar Pte Ltd. The vaccination process was carried out through subcutaneous injection in the neck and intramuscular injection in the pectoralis musculus in the chest area of chickens at 11 days of age, with a dose of 0.5 ml per chicken. The vaccine used was VAKSIMUNE® AI H9 which uses oil as the emulsion material. Results from the HI serology test showed that 6 cages from Myothar and Wah Net Chaung farms showed protective antibody titers against Avian Influenza H9N2 virus at 30-40 days of age, with normal titer values or ≥24. This indicates that the AI subtype H9N2vaccine used is an effective antigen, capable of stimulating the broiler immune system, and has implications for increasing antibodies every week. This study contributes to the understanding of AI H9N2 vaccination management in commercial broilers at JAPFA COMFEED Myanmar Pte Ltd. With positive results in the serology test, it can be concluded that vaccination using VAKSIMUNE® AI H9 is effective in protecting broilers from Avian Influenza H9N2 infection, providing strong support to the sustainability of production and animal health in a commercial farm environment.

Acidity and Alcohol Levels of Goat’s Milk Yoghurt with the Addition of Black Rice Bran Flour During Cold Storage Haskito, Ajeng Erika Prihastuti; Fatra, Ilhami Maulia; Mahdi, Chanif; Noviatri, Aldila; Adrenalin, Sruti Listra
JURNAL ILMIAH PETERNAKAN TERPADU Vol 12, No 3 (2024)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jipt.v12i3.p354-366

The development of yoghurt variations is increasingly diverse nowadays. The addition of black rice bran flour into yoghurt processing can increase the functional value of yoghurt, including increasing taste, antioxidant content, and dietary fiber. This research was to determine the effect of storage time in goat's milk yoghurt with the addition of black rice bran flour at a cold temperature of 4c on acidity and alcohol levels. The research used with 5 treatments, 4 repetitions. The treatment includes storage periods i.e., 0, 1, 2, 3 and 4 weeks. The acidity level will be calculated for each treatment group using the acid-base and alcohol titration method using the pycnometer method. The data obtained were analyzed using the one way ANOVA test followed by the Duncan Multiple Range Test. The results show that storage time has a very significant effect (p˂0.01) on acidity and alcohol levels. The highest acidity level was in week 2 (0.47%), then decreased continuously in weeks 3 and 4 of storage time. The alcohol content increased in line with storage time, reaching 0.0043% in week 4. If we look at the acidity and alcohol levels, the recommendation for consuming goat's milk yoghurt with the addition of black rice bran flour is no more than 1 weeks of storage time.

Komunikasi, Informasi Dan Edukasi Infeksi Penyakit Mulut Dan Kuku Pada Peternak Di KUD Kertajaya Kabupaten Kediri Putri, Salsabila Candra; Kurniawati, Siti; Adrenalin, Sruti Listra
Prosiding Seminar Nasional Unimus Vol 7 (2024): Transformasi Teknologi Menuju Indonesia Sehat dan Pencapaian Sustainable Development G
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar

Penyakit Mulut dan Kuku (PMK) merupakan penyakit yang disebabkan oleh virus Foot and Mouth DiseaseVirus (FMDV) genus Aphtovirus dan famili Picornaviridae. Penyakit Mulut dan Kuku merupakan penyakitmenular dan akut yang menyerang hewan ternak. Hewan ternak yang dapat terserang penyakit PMK adalahhewan berkuku genap atau berkuku belah seperti sapi, kambing, domba, kerbau, dan babi. Penyakit PMKmemiliki tingkat morbiditas mencapai 100% dan tingkat mortalitas sekitar 1.53% dari total kasus yang ada.Kegiatan KIE dilaksanakan di Desa Medowo, yang dikenal sebagai penghasil susu utama dengan sebagianbesar penduduknya bekerja sebagai peternak sapi perah. Kegiatan KIE bertujuan untuk meningkatkanpengetahuan dan pemahaman peternak terkait pencegahan dan penanganan infeksi PMK. Metode KIE yangdigunakan adalah menggunakan media informasi(pamflet), presentasi, diskusi secara langsung danpenilaian terukur dari hasil pretest, posttest, dan SKM yang dianalisis secara deskriptif dan kuantitatif. Hasilanalisis pretest dan posttest menunjukkan peningkatan yang signifikan, pretest menunjukkan terdapat 59%peternak yang memiliki nilai di atas standar. Setelah pelaksanaan kegiatan KIE, hasil posttest menunjukkanadanya peningkatan sebesar 41% dari hasil sebelumnya. Hasil pengisian SKM juga mendukung kegiatanpenyuluhan, peserta mengakui bahwa kegiatan tersebut relevan dan bermanfaat untuk memperluaspengetahuan para peternak. Kegiatan KIE mengenai PMK yang dilakukan kepada peternak di KUDKertajaya terbukti efektif dalam meningkatkan pemahaman peternak terhadap penyakit PMK. Kata Kunci : PMK, Komunikasi Informasi dan Edukasi, Peternak, KUD Kertajaya.

Detection of feline panleukopenia with antigen test kit Priambudi, M. Zuliono Dwi Risky; Haskito, Ajeng Erika Prihastuti; Inayah, Khoirul; Adrenalin, Sruti Listra
ARSHI Veterinary Letters Vol. 6 No. 1 (2022): ARSHI Veterinary Letters - Februari 2022
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.6.1.3-4

Feline panleukopenia is an infectious disease caused by the feline panleukopenia virus (FPV). The signs of infected cats in-clude fever, lethargy, anorexia, diarrhoea, and vomit. A secondary infection can exacerbate this condition, such as bacteria. The aim of this study to find out the diagnostic technique and the FPV detection method using the FPV antigen test kit. On August 2nd, 2021, the cat was brought to Griya Fauna Mojokerto with severe flu. In addition, based on the information, the cat has decreased appetite. The physical examination results that the temperature was 39.5 °C, bodyweight of 2.9 kg, and the cat looked weak when observed. Further, the FPV antigen test kit was used to confirm the diagnosis in this case. The FPV antigen test kit showed positive results that indicated by forming two lines on the test line (T) and the control line (C).

Total Lactic Acid Bacteria (LAB) and Antioxidant Activity of Goat's Milk Yoghurt with The Addition Of Red Bran During Refrigeration Storage Jangnga, Indah Puspadyah; Haskito, Ajeng Erika Prihastuti; Sari, Citra; Adrenalin, Sruti Listra
Journal of Applied Veterinary Science And Technology Vol. 4 No. 2 (2023): October 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V4.I2.2023.61-66

Background: Goat milk yogurt is a functional food made from processed food of animal origin with the help of LAB fermentation, which is healthy for the body. The utilization of red rice bran flour as an additional ingredient in making yogurt can increase the level of antioxidant activity and become a food fiber content in yogurt. Purpose: This study aims to determine the effect of refrigerated storage temperatures (4â„ƒ) of goat milk yogurt with the addition of red rice bran flour on total LAB and antioxidant activity. Methods: Using a CRD with five treatments and four replications. The treatment included 0, 1, 2, 3, and 4 weeks of storage. Each treatment group will be calculated for total LAB using the TPC method and antioxidant activity testing using the DPPH method. The data obtained were analyzed using the One Way ANOVA Test followed by the Duncan Test to determine the effect between treatment groups. Results: The results showed that storage time significantly affected (p<0.01) total LAB and antioxidant activity. The results of total LAB successively from 0 to 4 weeks of storage were 8,0 x 108 CFU/ml, 7,8 x 107 CFU/ml, 7,6 x 107 CFU/ml, 6,4 x 106 CFU/ml, and 6,3 x 106 CFU/ml. The results of antioxidant activity successively from 0 to 4 weeks of storage were 534,29 mg/ml, 443,96 mg/ml, 358,61 mg/ml, 282,32 mg/ml, and 230,88 mg/ml. Conclusion: Goat's milk yogurt with the addition of brown rice bran flour is recommended to be consumed for up to 2 weeks of storage.

Acidity and Alcohol Levels of Goat’s Milk Yoghurt with the Addition of Black Rice Bran Flour During Cold Storage Haskito, Ajeng Erika Prihastuti; Fatra, Ilhami Maulia; Mahdi, Chanif; Noviatri, Aldila; Adrenalin, Sruti Listra
JURNAL ILMIAH PETERNAKAN TERPADU Vol. 12 No. 3 (2024)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jipt.v12i3.p354-366

The development of yoghurt variations is increasingly diverse nowadays. The addition of black rice bran flour into yoghurt processing can increase the functional value of yoghurt, including increasing taste, antioxidant content, and dietary fiber. This research was to determine the effect of storage time in goat's milk yoghurt with the addition of black rice bran flour at a cold temperature of 4c on acidity and alcohol levels. The research used with 5 treatments, 4 repetitions. The treatment includes storage periods i.e., 0, 1, 2, 3 and 4 weeks. The acidity level will be calculated for each treatment group using the acid-base and alcohol titration method using the pycnometer method. The data obtained were analyzed using the one way ANOVA test followed by the Duncan Multiple Range Test. The results show that storage time has a very significant effect (p˂0.01) on acidity and alcohol levels. The highest acidity level was in week 2 (0.47%), then decreased continuously in weeks 3 and 4 of storage time. The alcohol content increased in line with storage time, reaching 0.0043% in week 4. If we look at the acidity and alcohol levels, the recommendation for consuming goat's milk yoghurt with the addition of black rice bran flour is no more than 1 weeks of storage time.

KOMUNIKASI INFORMASI DAN EDUKASI (KIE) PENYAKIT LUMPY SKIN DISEASE (LSD) PADA KELOMPOK TERNAK SAPI PERAH Eka Puji Dameanti, Fidi Nur Aini; Hendrawan, Viski Fitri; Oktanella, Yudit; Adrenalin, Sruti Listra; Amri, Indah Amalia; Kamulyan, Ujiati; Assifa Mardiyah, Fraya
RAMBIDEUN : Jurnal Pengabdian Kepada Masyarakat Vol. 7 No. 2 (2024): Rambideun: Jurnal Pengabdian Kepada Masyarakat
Publisher : LPPM Universitas Al Muslim

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51179/pkm.v7i2.2402

Lumpy Skin Disease is a smallpox disease on the skin caused by the Lumpy Skin Disease virus from the genus Capripox, family Poxviridae. The LSD virus is double a stranded deoxyribonucleic acid (DNA virus), has a lipid envelope, and replicates in the cytoplasm. This disease can infect cattle and buffalo, and give an economic impact on farmers. Information about LSD in Indonesia has not been published for a long time and has not been published enough, so the Faculty of Veterinary Medicine, Brawijaya University has taken the role of conveying information about LSD through Communication, Information and Education (KIE) media in order to increase awareness of the entry and spread of LSD in areas experiencing LSD outbreaks. The KIE activity was attended by 17 participants from the "Sukses Bersama" Dairy Cattle Group. The results obtained from KIE activities were an increase in participants' knowledge, known from the results of the pretest and posttest analysis. The highest average score before the presentation of the material, the teenage group respondents answered 10 questions correctly, the adult and elderly groups answered 11 questions correctly. Meanwhile, after the presentation of the material, the highest average of respondents in the teenage, adult and elderly groups answered 12 questions correctly. The participant's age influences the participant's ability to answer questions correctly.

Komunikasi, Informasi Dan Edukasi Infeksi Penyakit Mulut Dan Kuku Pada Peternak Di KUD Kertajaya Kabupaten Kediri Putri, Salsabila Candra; Kurniawati, Siti; Adrenalin, Sruti Listra
Prosiding Seminar Nasional Unimus Vol 7 (2024): Transformasi Teknologi Menuju Indonesia Sehat dan Pencapaian Sustainable Development G
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar

Penyakit Mulut dan Kuku (PMK) merupakan penyakit yang disebabkan oleh virus Foot and Mouth DiseaseVirus (FMDV) genus Aphtovirus dan famili Picornaviridae. Penyakit Mulut dan Kuku merupakan penyakitmenular dan akut yang menyerang hewan ternak. Hewan ternak yang dapat terserang penyakit PMK adalahhewan berkuku genap atau berkuku belah seperti sapi, kambing, domba, kerbau, dan babi. Penyakit PMKmemiliki tingkat morbiditas mencapai 100% dan tingkat mortalitas sekitar 1.53% dari total kasus yang ada.Kegiatan KIE dilaksanakan di Desa Medowo, yang dikenal sebagai penghasil susu utama dengan sebagianbesar penduduknya bekerja sebagai peternak sapi perah. Kegiatan KIE bertujuan untuk meningkatkanpengetahuan dan pemahaman peternak terkait pencegahan dan penanganan infeksi PMK. Metode KIE yangdigunakan adalah menggunakan media informasi(pamflet), presentasi, diskusi secara langsung danpenilaian terukur dari hasil pretest, posttest, dan SKM yang dianalisis secara deskriptif dan kuantitatif. Hasilanalisis pretest dan posttest menunjukkan peningkatan yang signifikan, pretest menunjukkan terdapat 59%peternak yang memiliki nilai di atas standar. Setelah pelaksanaan kegiatan KIE, hasil posttest menunjukkanadanya peningkatan sebesar 41% dari hasil sebelumnya. Hasil pengisian SKM juga mendukung kegiatanpenyuluhan, peserta mengakui bahwa kegiatan tersebut relevan dan bermanfaat untuk memperluaspengetahuan para peternak. Kegiatan KIE mengenai PMK yang dilakukan kepada peternak di KUDKertajaya terbukti efektif dalam meningkatkan pemahaman peternak terhadap penyakit PMK. Kata Kunci : PMK, Komunikasi Informasi dan Edukasi, Peternak, KUD Kertajaya.

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