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Prevalence and Predisposing factors of Subclinical Mastitis in Dairy Cows in Bakir, Sukomulyo Village, Batu Regency Fatmawati, Mira; Setianingrum, Ani; Haskito, Ajeng Erika Prihastuti; Dameanti, Fidi Nur Aini Eka Puji
Veterinary Biomedical and Clinical Journal Vol. 1 No. 2 (2019)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Peradangan ambing pada sapi perah yang lebih dikenal sebagai mastitis masih menjadi masalah utama dalam industri susu. Kerugian peternak karena mastitis disebabkan oleh penurunan produksi susu dan penurunan kualitas susu yang dihasilkan. Pada umumnya pengetahuan peternak rakyat akan mastitis sebatas pada mastitis klinis karena gejala klinis yang dapat teramati. Mastitis yang sifatnya subklinis sulit dideteksi dan apabila dibiarkan tanpa penanganan dapat menjadi sumber penularan ke sapi perah lainnya. Deteksi mastitis subklinis pada sapi perah menggunakan uji tidak langsung yaitu california mastitis test (CMT) atau menggunakan uji langsung dengan penghitungan jumlah sel somatis (JSS). Penelitian ini merupakan studi lintas seksional dengan menggunakan sampel susu kwartir pada peternakan sapi perah rakyat di dusun Bakir, desa Sukomulyo, kabupaten Batu. Pengujian mastitis dilakukan di lapangan dengan uji CMT dan di laboratorium untuk penghitungan JSS. Pengambilan data dengan kuesioner untuk mengetahui faktor predisposisi mastitis. Prevalensi mastitis subklinis berdasarkan JSS adalah 60,22% dan berdasarkan uji CMT di lapangan adalah 18,20% dari total sampel kwartir. Faktor predisposisi yang mempengaruhi masih adanya kejadian mastitis pada peternakan antara lain kebersihan sapi, kandang, dan sanitasi sebelum dan sesudah pemerahan.

SUPLEMENTASI YOGURT FORTIFIKASI KONSENTRASI BERTINGKAT EKSTRAK ROSELLA UNGU TERHADAP KADAR MALONDIALDEHIDA (MDA) DAN GAMBARAN HISTOLOGI GINJAL MENCIT Ratna, Novia Kumala Dian Nur; Setianingrum, Ani; Nugroho, Widi; Haskito, Ajeng Erika Prihastuti; Noviatri, Aldila
JURNAL KAJIAN VETERINER Vol 10 No 1 (2022): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v10i1.5504

Purple roselle has ingredients such as organic acids, anthocyanins, polysaccharides, and flavonoids. The antioxidant content in purple roselle has benefits in preventing cell damage due to free radicals. This study was conducted to discoverthe effect of supplementation yogurt fortified with a graded concentration of purple roselle extract on MDA levels and histology of the kidney organs of mice. Laboratory experimental study using 16 male Balb-C mice divided into four groups. The control (C) group was not given any treatment. The treatment groups 1,2, and 3 (T1, T2, T3) were given fortified purple roselle yogurt with concentrations of 5%, 10%, and 15%, respectively. The treatment was carried out for 14 days with a volume of 0.4 ml/day. Kidney organs were collected for histological analysis. Kidney malondialdehydelevels were assayed using the TBARS test and statistically analyzed with a 95% confidence level by one-way ANOVA analysis. Histology score analyzed using the Kruskal-Wallis test followed by the Mann- Whitney test. This study showedthat at concentrations of 10% and 15%, MDA levels of the mice kidneys reduce significantly compared to the control group. The kidney histology score results show some changes in treatment groups compares to control group. The average score decreasing from different treatment group, and the lowest score was group given yogurt fortified roselle extract 15%. The administration of fortified yogurt with purple roselle extract at a concentration of 15% can reduce MDA levels in the kidneys of mice, and the higher the concentration can reduce kidney damage in mice.

Pengaruh Penyimpanan Suhu Refrigerasi terhadap Karakteristik Fisik Organoleptik, Nilai pH, Cemaran Coliform dan Salmonella spp., Yoghurt Susu Kambing dengan Penambahan Tepung Bekatul Beras Merah Haskito, Ajeng Erika Prihastuti; Mahdi, Chanif; Mawarni, Riski Eka; Immauliate, Sinthia Medchera
JURNAL KAJIAN VETERINER Vol 12 No 1 (2024): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v12i1.7946

Yogurt is a fermented milk product made with the help of Lactic Acid Bacteria (LAB). Goat milk yogurt with the addition of red rice bran flour contains high antioxidants, making it very beneficial for health. This study aims to evaluate the effect of storage duration on the organoleptic physical characteristics, pH value, and contamination by Coliform and Salmonella spp. in goat milk yogurt with the addition of red rice bran flour. The study used 20 samples divided into five treatment groups based on storage duration (0, 1, 2, 3, and 4 weeks) at 4 ℃, with four repetitions. Each week, organoleptic physical characteristics were tested hedonically and hedonic quality assessed by 20 semi-trained panellists, pH measured with a digital pH meter, Coliform contamination tested using the Most Probable Number (MPN) method, and Salmonella spp. contamination tested using SSA media followed by TSIA media. Data analysis for organoleptic physical characteristics was conducted using the Kruskal-Wallis test followed by the Mann-Whitney test. Data analysis for pH and Coliform contamination was conducted using one-way ANOVA followed by the Tukey test. Data analysis for Salmonella spp. contamination was done descriptively. The results showed that the storage duration significantly affected the physical characteristics of appearance, taste, and aroma (p<0.05) but did not significantly affect colour (p>0.05). There were very significant differences in pH values and Coliform contamination (p<0.01), whereas Salmonella spp. contamination was not affected by the storage duration. The conclusion of this study is that goat milk yogurt with the addition of red rice bran flour should be consumed within three weeks based on organoleptic physical characteristics, pH value, and levels of Coliform and Salmonella spp. contamination.

Purple Roselle Fortified Yogurt Supplementation Prevents Dioxin-Induced Oxidative Testicular Impairment in Rats Setianingrum, Ani; Nindhyasari, Shintya; Pratiwi, Herlina; Haskito, Ajeng Erika Prihastuti; Noviatri, Aldila
The Journal of Experimental Life Science Vol. 14 No. 2 (2024)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2024.014.02.03

Dioxins are highly toxic and hurt multiple organs and systems, including the reproductive organs. This study determined the supplementation of purple roselle (Hibiscus sabdariffa var. altissima) extract into yogurt to prevent testicular deterioration induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). The fortification of purple roselle extract into yogurt (RY) was used as a supplement because bioactive components are known to be efficacious in preventing cell damage. Five groups of male Wistar rats were group as follows: 1) negative control (C), 2) TCDD (T), 3) TCDD and 0.5% RY (P1), 4) TCDD and 1% RY (P2), 5) TCDD and 1.5% RY (P3). All treatments were carried out orally for 12 days. Testicular Malondialdehyde (MDA) level and histopathological changes were described quantitatively by one-way ANOVA. The study's results showed that treatment groups P1 (0.5% RY) and P2 (1% RY) had significantly (P<0,05) lower MDA levels than the T group, while group P3 had no significant decrease in MDA level. It proved that supplementation of purple roselle yogurt reduced the formation of MDA in testicular tissue. Histopathology changes show that all groups exposed to TCDD have depleted spermatogenic, Sertoli, and Leydig cells compared to the C group. Suggests that dioxin exposure can have adverse effects on male reproductive organs. This research concludes that supplementing yogurt fortified with purple roselle extract can prevent increased testicular MDA levels. The decrease in testicular cells due to TCDD toxicity was inevitable. However, antioxidant activity from roselle yogurt supplementation can prevent further damage in rat testicular cells. Keywords: Histopathology, MDA, Purple Roselle, Testis, TCDD, Yogurt.

Acidity and Alcohol Levels of Goat’s Milk Yoghurt with the Addition of Black Rice Bran Flour During Cold Storage Haskito, Ajeng Erika Prihastuti; Fatra, Ilhami Maulia; Mahdi, Chanif; Noviatri, Aldila; Adrenalin, Sruti Listra
JURNAL ILMIAH PETERNAKAN TERPADU Vol 12, No 3 (2024)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jipt.v12i3.p354-366

The development of yoghurt variations is increasingly diverse nowadays. The addition of black rice bran flour into yoghurt processing can increase the functional value of yoghurt, including increasing taste, antioxidant content, and dietary fiber. This research was to determine the effect of storage time in goat's milk yoghurt with the addition of black rice bran flour at a cold temperature of 4c on acidity and alcohol levels. The research used with 5 treatments, 4 repetitions. The treatment includes storage periods i.e., 0, 1, 2, 3 and 4 weeks. The acidity level will be calculated for each treatment group using the acid-base and alcohol titration method using the pycnometer method. The data obtained were analyzed using the one way ANOVA test followed by the Duncan Multiple Range Test. The results show that storage time has a very significant effect (p˂0.01) on acidity and alcohol levels. The highest acidity level was in week 2 (0.47%), then decreased continuously in weeks 3 and 4 of storage time. The alcohol content increased in line with storage time, reaching 0.0043% in week 4. If we look at the acidity and alcohol levels, the recommendation for consuming goat's milk yoghurt with the addition of black rice bran flour is no more than 1 weeks of storage time.

Preventive Effect of Antioxidant From Purple Rosella Yoghurt on Duodenum and Colon Histopathology of Rattus norvegicus Exposed to Rhodamine B And Saccharin Haskito, Ajeng Erika Prihastuti; Nariswari, Bintari; Safitri, Anna; Noviatri, Aldila; Untari, Handayu; Sari, Citra
The Journal of Experimental Life Science Vol. 14 No. 1 (2024)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2024.014.01.04

Rodhamine B and Saccharin are often misused by food producers as food additive, which, if their use is not monitored, can produce free radicals that cause oxidative stress conditions in the body, leading to dangerous damage to the body. The addition of purple rosella in yoghurt can increase the functional value of the yoghurt, namely increasing the antioxidant content. The purpose of this study was to determine the benefits of giving purple rosella yoghurt in preventing damage to the duodenum and colon of (Rattus norvegicus) caused by Rodhamine B and Saccharin. This study used Completely Randomized Design (CRD) with 28 male R. novergicus, Wistar strain in aged 8-12 weeks. Group K was not given any treatment; R was given Rodhamine B, S was given Saccharin, KRS was given Rodhamine B and Saccharin, YR was given Rodhamine B and yoghurt, YS was given Saccharin and yogurt, YRS was given Rodhamine B, Saccharin and yogurt. The dose of Rodhamine B was 22.5 mg.kg-1, Saccharin was 157.77 mg.kg-1, and the volume of yoghurt was 1 mL.head-1. They were administered orally with a gastric tube for 14 days. The concentration of purple rosella extract added to yoghurt was 15% (v/v). Parameters were observed in histopathology of the duodenum and colon with the scoring method. Data analysis was done with the Kruskal-Wallis test followed by the Mann-Whitney test. The KRS group showed the heaviest damage, namely necrosis, villi erosion, and inflammatory cell infiltration of >75% of the total visual field in the duodenum and colon. The YR, YS, and YRS groups showed significant changes compared to the R, S, and KRS groups, namely necrosis, villous erosion, and inflammatory cell infiltration, which appeared to be 25-50% of the total visual field. However, this did not match the condition of group K, which appeared to be <25% of the total visual field. The provision of purple rosella yoghurt can prevent 50% damage to the duodenum and colon of Rattus novergicus due to Rodhamine B and Saccharin. Keywords: colon, duodenum, Rodhamine B, Saccharin, yoghurt.

Pembelajaran Pembuatan Yoghurt Bekatul Beras Putih, Merah, dan Hitam Sebagai Alternatif Diet Kondisi Diabetes Melitus pada Masyarakat Desa Kepuharjo Kabupaten Malang Haskito, Ajeng Erika Prihastuti
TRI DHARMA MANDIRI: Diseminasi dan Hilirisasi Riset kepada Masyarakat (Jurnal Pengabdian kepada Masyarakat) Vol 5, No 1 (2025)
Publisher : JTRIDHARMA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtridharma.2025.005.01.43

Yoghurt merupakan pangan fungsional dengan kandungan gizi kompleks yang bermanfaat bagi kesehatan tubuh. Penambahan bekatul beras putih, merah, dan hitam pada pembuatan yoghurt dapat meningkatkan kandungan gizi yoghurt karena bekatul memiliki kandungan serat pangan yang tinggi dan komponen bioaktif seperti antosianin. Sebuah penelitian menunjukkan hasil terapi yoghurt bekatul beras putih, merah, dan hitam secara efektif dapat menurunkan kadar glukosa darah pada penderita diabetes melitus melalui mekanisme penghambatan enzim α-glukosidase oleh bakteri asam laktat dan peningkatan aktivitas GLUT4 oleh antosianin. Pengabdian kepada masyarakat di Desa Kepuharjo Kabupaten Malang ini meliputi edukasi manfaat yoghurt bekatul beras putih, merah, dan hitam dilengkapi dengan pembelajaran pembuatannya. Hasil kegiatan ini menunjukan adanya peningkatan pemahaman dan kemampuan dalam pembuatan yoghurt bekatul beras putih, merah, dan hitam yang signifikan pada masyarakat.

Pembelajaran Pembuatan Yoghurt Bekatul Beras Putih, Merah, dan Hitam Sebagai Alternatif Diet Kondisi Diabetes Melitus pada Masyarakat Desa Kepuharjo Kabupaten Malang Haskito, Ajeng Erika Prihastuti
TRI DHARMA MANDIRI: Dissemination and Downstreaming of Research to the Community (Journal of Community Engagement) Vol 5 No 1 (2025)
Publisher : SMONAGENES Research Center, Univeritas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtridharma.2025.005.01.43

Yoghurt merupakan pangan fungsional dengan kandungan gizi kompleks yang bermanfaat bagi kesehatan tubuh. Penambahan bekatul beras putih, merah, dan hitam pada pembuatan yoghurt dapat meningkatkan kandungan gizi yoghurt karena bekatul memiliki kandungan serat pangan yang tinggi dan komponen bioaktif seperti antosianin. Sebuah penelitian menunjukkan hasil terapi yoghurt bekatul beras putih, merah, dan hitam secara efektif dapat menurunkan kadar glukosa darah pada penderita diabetes melitus melalui mekanisme penghambatan enzim α-glukosidase oleh bakteri asam laktat dan peningkatan aktivitas GLUT4 oleh antosianin. Pengabdian kepada masyarakat di Desa Kepuharjo Kabupaten Malang ini meliputi edukasi manfaat yoghurt bekatul beras putih, merah, dan hitam dilengkapi dengan pembelajaran pembuatannya. Hasil kegiatan ini menunjukan adanya peningkatan pemahaman dan kemampuan dalam pembuatan yoghurt bekatul beras putih, merah, dan hitam yang signifikan pada masyarakat.

Detection of feline panleukopenia with antigen test kit Priambudi, M. Zuliono Dwi Risky; Haskito, Ajeng Erika Prihastuti; Inayah, Khoirul; Adrenalin, Sruti Listra
ARSHI Veterinary Letters Vol. 6 No. 1 (2022): ARSHI Veterinary Letters - Februari 2022
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.6.1.3-4

Feline panleukopenia is an infectious disease caused by the feline panleukopenia virus (FPV). The signs of infected cats in-clude fever, lethargy, anorexia, diarrhoea, and vomit. A secondary infection can exacerbate this condition, such as bacteria. The aim of this study to find out the diagnostic technique and the FPV detection method using the FPV antigen test kit. On August 2nd, 2021, the cat was brought to Griya Fauna Mojokerto with severe flu. In addition, based on the information, the cat has decreased appetite. The physical examination results that the temperature was 39.5 °C, bodyweight of 2.9 kg, and the cat looked weak when observed. Further, the FPV antigen test kit was used to confirm the diagnosis in this case. The FPV antigen test kit showed positive results that indicated by forming two lines on the test line (T) and the control line (C).

Total Lactic Acid Bacteria (LAB) and Antioxidant Activity of Goat's Milk Yoghurt with The Addition Of Red Bran During Refrigeration Storage Jangnga, Indah Puspadyah; Haskito, Ajeng Erika Prihastuti; Sari, Citra; Adrenalin, Sruti Listra
Journal of Applied Veterinary Science And Technology Vol. 4 No. 2 (2023): October 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V4.I2.2023.61-66

Background: Goat milk yogurt is a functional food made from processed food of animal origin with the help of LAB fermentation, which is healthy for the body. The utilization of red rice bran flour as an additional ingredient in making yogurt can increase the level of antioxidant activity and become a food fiber content in yogurt. Purpose: This study aims to determine the effect of refrigerated storage temperatures (4â„ƒ) of goat milk yogurt with the addition of red rice bran flour on total LAB and antioxidant activity. Methods: Using a CRD with five treatments and four replications. The treatment included 0, 1, 2, 3, and 4 weeks of storage. Each treatment group will be calculated for total LAB using the TPC method and antioxidant activity testing using the DPPH method. The data obtained were analyzed using the One Way ANOVA Test followed by the Duncan Test to determine the effect between treatment groups. Results: The results showed that storage time significantly affected (p<0.01) total LAB and antioxidant activity. The results of total LAB successively from 0 to 4 weeks of storage were 8,0 x 108 CFU/ml, 7,8 x 107 CFU/ml, 7,6 x 107 CFU/ml, 6,4 x 106 CFU/ml, and 6,3 x 106 CFU/ml. The results of antioxidant activity successively from 0 to 4 weeks of storage were 534,29 mg/ml, 443,96 mg/ml, 358,61 mg/ml, 282,32 mg/ml, and 230,88 mg/ml. Conclusion: Goat's milk yogurt with the addition of brown rice bran flour is recommended to be consumed for up to 2 weeks of storage.

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