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All Journal Majalah Kedokteran Bandung Jurnal Kedokteran Hewan Jurnal Veteriner
Lubis, Alkaustariyah
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Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Old Mice Epididymal Sperm Quality After Short Term Gavage of Cogon Grass Root Ethanol Extract Lubis, Alkaustariyah; Widyastuti, Rini; Robianto, Sondi; Priscilla, Madeleine; Syamsunarno, Mas Rizky A. A.
Majalah Kedokteran Bandung Vol 50, No 2 (2018)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v50n2.1238

Abstract

Cogon grass (Imperata cylindrica) has been known as traditional herb because of its antioxidant activity. The oxidant level in the body increases linearly with age. Antioxidant works by decreasing the vulnerability of mammalian spermatozoa towards free radical attack. This study aimed to determine whether the active compound of Imperata cyclindrica root extract improved or damaged  sperm function in old mice. This was a complete randomized study performed in the Mouse Animal Laboratory Faculty of Medicine, Universitas Padjadjaran during the period of August to December 2017. This study was carried out by oral administration of three different doses i.e 115, 230, and 345 mg/kg body weight of cogon grass root ethanol extract into 24- weeks-old DDY strain mice for 14 days. Results showed no significant difference in body weight  and testicular index before and after treatment (p<0.05). Interestingly, sperm concentration decreased significantly in 345 mg/kgBW group when compared  to control (2.03x106 and 6.43x106 respectively, p<0.05). On the other hand, sperm motility and sperm with normal morphology increased following dose pattern, although not significantly, when compared to control (p<0.05). Hence, cogon grass root ethanol extract cannot maintain optimum epididymal sperm quality in old mice.  Key words: Aging, Imperata cylindrica, sperm quality Kualitas Sperma Epididimis Mencit Tua setelah Pemberian Ekstrak Akar Alang-Alang secara Oral Jangka PendekAlang-alang (Imperata cylindrica) dikenal sebagai obat tradisional karena efek antioksidan yang dimiliki. Kadar oksidan pada tubuh meningkat seiring pertambahan usia. Antioksidan bekerja dengan cara mengurangi kerentanan spermatozoa terhadap radikal bebas. Penelitian ini bertujuan untuk menentukan apakah komponen aktif ekstrak akar alang-alang dapat memperbaiki atau merusak fungsi sperma pada mencit tua. Penelitian ini dilakukan di Laboratorium Hewan Fakultas Kedokteran Universitas Padjadjaran periode Agustus hingga Desember 2017. Eksperimen dibagi menjadi satu grup kontrol dan tiga grup perlakuan dengan dosis 115, 230 dan 345 mg/kgBB selama 14 hari. Hasil menunjukkan tidak ada perbedaan signifikan pada berat badan dan index testis sebelum dan sesudah perlakuan. Menariknya, jumlah sperma menurun dengan signifikan pada kosentrasi tertinggi 345 mg/kgBB dibanding kontrol, yaitu 2,03x106 dan 6,43x106 (p<0.05). Selain itu, terjadi peningkatan motilitas sperma dan morfologi normal secara gradual hingga dosis tertinggi dibanding dengan kontrol walaupun tidak signifikan. Berdasar atas hasil yang didapat, ekstrak akar alang-alang dapat menjaga kualitas sperma pada mencit tua walaupun tidak optimal.Kata kunci: Imperata cylindrica, kualitas sperma, penuaan 
THE EFFECT OF COGON GRASS ROOT ETHANOL EXTRACT ON REPRODUCTIVE ORGAN AND EPIDIDYMAL SPERM QUALITY OF MOUSE WITH DIFFERENT AGES Sudiman, Jaqueline; Widyastuti, Rini; Lubis, Alkaustariyah; Hartady, Tyagita; Syamsunarno, Mas Rizky Anggun Adipurna; Sumarsono, Sony Heru
Jurnal Kedokteran Hewan Vol 14, No 1 (2020): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v14i1.14969

Abstract

This study aims to determine changes related to epididymal sperm parameters in mice given cogon grass root ethanol extract (CGREE). The experiment was carried out by dividing three groups of mice aged 4-week old (early pubertal), 8-week old (adult), and 24-week old (old) as control and treatment groups. The treatment groups were given 115 mg/kg body weight of CGREE for 14 days. The mice were sacrificed on day 15 to asses reproductive organ indexes and quality of the epididymal sperm. Index of epididymis and ductus deferens indices significantly reduced in the 4-week old treatment group compared to control (0.23 vs 0.33; 0.08 vs 0.16, respectively), and vesical seminal significantly reduced in 8- week old treatment group compared to control (0.76 vs 0.68). The epididymal sperm quality significantly decreased in all treatment groups, but the sperm concentration in early pubertal group showed an increase. These results indicate that CGREE can enhance several semen parameters in prepubertal and adult mice but this effect is not apparent in old mice.
Vaginal Cytological Changes and Estrus Response Following Estrus Synchronization Using a Double Prostaglandin-F₂α and Gonadotropin Releasing Hormone in Pasundan Heifers Rini Widyastuti; Azis, Azhar Abdul; Setiawan, Rangga; Hilmia, Nena; Lubis, Alkaustariyah; Ridlo, Muhammad Rosyid
Jurnal Veteriner Vol. 27 No. 1 (2026)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/

Abstract

Pasundan cattle, an indigenous breed native to West Java, Indonesia, demonstrate significant adaptability and reproductive potential. However, their declining populations pose a threat to genetic conservation. Effective estrus synchronization is essential for enhancing reproductive efficiency and supporting fixed-time artificial insemination (FTAI) programs. Vaginal cytology profiling offers a reliable, noninvasive method for assessing estrus by evaluating the dynamics of epithelial and leukocyte cells, which reflect hormonal fluctuations. This study was aimed to investigate the cytological dynamics of vaginal epithelial, leukocyte cells and estrus response in Pasundan heifers subjected to double injection of prostaglandin F₂α (PGF₂α) and gonadotropin-releasing hormone (GnRH) synchronization. Nine Pasundan heifers, aged 1.5–2.0 years, were treated with PGF₂α with 2.75 μg/kg BW on days 0 and 11, followed by 2 μg/kg BW GnRH on day 12. Vaginal smears were collected daily from days 0 to 15, stained with 5% Giemsa, and examined microscopically to determine the proportions of parabasal, intermediate, superficial, keratinized, and leukocyte cells. The results indicated that the initial cytology showed a luteal phase dominated by parabasal (31.2%) and intermediate (30.6%) cells. Following hormonal treatment, superficial cells peaked at 70.9% on day 13, indicating estrus and estrogen dominance. By days 14–15, keratinized and parabasal cells had increased, confirming post-ovulatory luteal activity. The protocol achieved complete (100%) estrus synchronization among all heifers. It can be concluded that the double PGF₂ α-GnRH protocol effectively synchronized estrus in Pasundan heifers. Vaginal cytology has proven to be a sensitive and practical tool for monitoring reproductive phases, thereby supporting breeding management and conservation of this native genetic resource.
Co-Authors Azis, Azhar Abdul Mas Rizky A.A. Syamsunarno Nena Hilmia Priscilla, Madeleine Rangga Setiawan Ridlo, Muhammad Rosyid Rini Widyastuti Robianto, Sondi SONY HERU SUMARSONO Sudiman, Jaqueline Syamsunarno, Mas Rizky A. A. Tyagita Hartady