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All Journal Majalah Kedokteran Gigi Indonesia Interdental Jurnal Kedokteran Gigi (IJKG) International Journal of Applied Science and Sustainable Development (IJASSD)
Dewi Farida Nurlitasari
Department Of Prostodontic, Faculty Of Dentistry, Mahasaraswati Denpasar University
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Dentistry Education Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Public Health

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Vitamin D inhibits TNF-α serum level in wistar rats stimulated with Porphyromonas gingivalis Hervina, Hervina; Syahriel, Dwis; Wedagama, Dewa Made; Nurlitasari, Dewi Farida; Nasutianto, Haris; Haryani, I Gusti Ayu Dewi
Majalah Kedokteran Gigi Indonesia Vol 9, No 3 (2023): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.81563

Abstract

Periodontitis is a chronic inflammation of the periodontal tissues initiated by increased proinflammatory cytokines such as TNF-α due to the induction of Porphyromonas gingivalis (Pg). This study aimed to analyze the effect of vitamin D (cholecalciferol) on TNF-α serum level in Wistar rats stimulated with Pg to induce periodontitis. Twenty-seven male Wistar rats (n = 27) were divided into three equal groups. Group I was healthy Wistar rats that received 2000 IU vitamin D once a day. Group II was Wistar rats stimulated with Pg and received 2000 IU vitamin D, while group III was Wistar rats stimulated with Pg but did not receive vitamin D. Blood was collected through the orbital sinus and centrifuged to get the serum. TNF-α serum levels were assessed using Elisa method on the 7th, 14th, and 28th. The data were normally distributed and homogeneous. The mean TNF-α data was analyzed for differences between groups using the one-way ANOVA and LSD post hoc test. Significant differences were seen in group II (418.49 ± 161.08 ng/mL) and group III (172.16 ± 104.18 ng/mL) on the 28th day (p = 0.001). The findings suggest that vitamin D inhibits the TNF-α serum level in Wistar rats stimulated with Pg on the 28th day.
Antifungal Potential of Sansevieria Trifasciata Infusion as a Cleaner for Dentures Dewi, Tri Purnami; Nurlitasari, Dewi Farida; Putra, Bagus Eda Purnariyawan
Interdental Jurnal Kedokteran Gigi (IJKG) Vol. 21 No. 2 (2025): Interdental Jurnal Kedokteran Gigi (IJKG)
Publisher : Fakultas Kedokteran Gigi, Universitas Mahasaraswati Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46862/interdental.v21i2.12202

Abstract

Introduction: Thermoplastic nylon is one of the alternative materials used for denture bases, in addition to acrylic resin. It offers good flexibility and aesthetics; however, its high water absorption makes it susceptible to microbial colonization. Sansevieria trifasciata is a herbal plant known for its antiseptic, antibacterial, and antifungal properties. Therefore, it has the potential to be developed as a natural denture cleanser. This study aims to determine the antifungal effect of a 40% S. trifasciata var. laurentii infusion on the growth of Candida albicans colonies on thermoplastic nylon denture base plates. Material And Methods: This in vitro experimental study used a post-test only control group design with four groups: one negative control (sterile distilled water) and three groups treated with 40% S. trifasciata infusion for 24, 48, and 72 hours. A Candida albicans suspension (1.5 × 10⁸ CFU/mL) was prepared using McFarland standard 0.5. Phytochemical screening was conducted to identify active compounds in the extract. Data were analyzed using One-Way ANOVA and LSD post hoc test (p < 0.05). Results and Discussions: Phytochemical analysis of the S. trifasciata var. laurentii extract indicated the presence of saponins, phenols, flavonoids, and alkaloids, all of which are known to exhibit antifungal activity. The descriptive analysis showed that the treatment group with 72-hour immersion had the lowest average colony count of C. albicans. Statistical analysis using One-Way ANOVA showed a significant difference among the groups (p = 0.001). LSD post hoc analysis revealed that all treatment groups differed significantly from the control group (p < 0.05). These results indicate that prolonged immersion in S. trifasciata var. laurentii infusion enhances its antifungal efficacy. The observed antifungal activity is likely related to the disruption of fungal cell membranes by the active compounds present in the extract. Conclusion: The 40% S. trifasciata var. laurentii infusion is effective in inhibiting the growth of C. albicans on thermoplastic nylon denture base plates. The most significant antifungal effect was observed with a 72-hour immersion duration.
Effect of Arenga pinnata Palm Wine on Surface Roughness of Acrylic and Thermoplastic Nylon: SEM Study Dewi, Tri Purnami; Nurlitasari, Dewi Farida; Putri, Ni Putu Dita Kristiana
Interdental Jurnal Kedokteran Gigi (IJKG) Vol. 22 No. 1 (2026): Interdental Jurnal Kedokteran Gigi (IJKG)
Publisher : Fakultas Kedokteran Gigi, Universitas Mahasaraswati Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46862/interdental.v22i1.13006

Abstract

Introduction: Surface roughness is an important property of denture base materials because it affects hygiene, comfort, and durability. Rough surfaces can retain debris and microorganisms, increasing the risk of halitosis and mucosal irritation. Tuak aren (Arenga pinnata palm wine), a traditional fermented beverage, contains alcohol and has an acidic pH that may alter denture base materials. This study evaluated the effect of tuak aren on the surface roughness of acrylic and thermoplastic nylon denture bases. Materials and Methods: Four groups were studied: acrylic plates (A1) and thermoplastic nylon plates (A2) immersed in distilled water as controls, and acrylic plates (B1) and thermoplastic nylon plates (B2) immersed in tuak aren as treatments. After 11 days of immersion, surface morphology was examined using scanning electron microscopy (SEM), and surface roughness was measured using a profilometer. Results: Descriptive analysis showed that A1 had the lowest surface roughness, while B2 had the highest. One-way ANOVA demonstrated significant differences among groups. LSD post hoc analysis revealed significant differences between B2 and groups A1, A2, and B1. SEM observations at 500× magnification showed microscopic surface changes after immersion in tuak aren. Profilometer measurements were consistent with SEM findings. Conclusion: Exposure to tuak aren increased the surface roughness of denture base materials, with the greatest effect observed in thermoplastic nylon plates.
Co-Authors A.A.A. Hartini Dewa Made Wedagama Dewi, TP Dewi, Tri Purnami Dsk Pt Bunga Raditya Pradnyadena Dwis Syahriel Haris Nasutianto Hervina I G A Pt Asri Septyanti I GUSTI AYU ARI AGUNG I Gusti Ayu Dewi Haryani I Gusti Ayu Putu Mahendri I Nyoman Panji Triadnya Palgunadi Nugrahini, Sintha Putra, Bagus Eda Purnariyawan Putri, Ni Putu Dita Kristiana Septyanti, I G A Pt Asri Suhendra TP Dewi