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Estrous Cycle Response in Mice (Mus musculus) with CSE (Crude Sperm Extract) Injected Intraperitoneally Mutiati, Enni; Rahayu, Sri; Ciptadi, Gatot; Nasich, Moch.
The Journal of Experimental Life Science Vol. 6 No. 1 (2016)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (976.426 KB) | DOI: 10.21776/ub.jels.2016.006.01.03

Abstract

Sperm protein has an important role in fertilization process. It becomes antigenic when it is injected to body and can increase TNF-α secretion. TNF-α in blood vesssel can inhibit estradiol synthesis. Estradiol has a significant role in repduction cycle, especially in estrous cycle. The study aims to understand the influence of Crude Sperm Extract (CSE) to mice (Mus musculus) estrous cycle. 16 fertile mices strain Balb-C aged 3-4 months, weighed 20-30 g was divided into 4 groups. P0 is a control group injected by PBS, group P1, P2, and P3 injected by CSE with 1.5 mg.ml-1, 2 mg.ml-1, and 2.5 mg.ml-1. CSE is injected intraperitoneally during mice's  diestrus phase. CSE is injected in day 0, day 12, day 24 and observed daily. The data are descriptively analyzed. The results show that CSE with molecule weight between 26.8-176.8 kDa influences estrous cycle. Keywords: Crude Sperm Extract, estrous cycle, folliculogenesis, Mus musculus
The effects of combined black pepper extract and yogurt on histology of mice digestive organs Mutiati, Enni; Inayah, Nurul; Putri, Kanza Nabila
JPBIO (Jurnal Pendidikan Biologi) Vol 11, No 1 (2026): April 2026
Publisher : STKIP Persada Khatulistiwa Sintang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31932/jpbio.v11i1.5881

Abstract

Black pepper contains piperine, which can irritate the digestive organs. Therefore, this study combined black pepper with yogurt containing probiotic bacteria, which are known to play a role in maintaining the balance of microbiota and digestive tract health. The objective of this study was to analyze the histopathology and histomorphometry of the digestive organs, particularly the stomach and small intestine, of mice (Mus musculus) after administration of a combination of black pepper extract and yogurt. The present study was conceptualized as an experimental study, employing a completely randomized design (CRD), encompassing five distinct treatments (distilled water, yogurt, a black pepper extract at a dose of 25, 50, 75 mg/kg BB, and yogurt. The results demonstrated that the thickness of the stomach wall in all treatment groups remained within the normal range. Treatment P2 demonstrated optimal outcomes, exhibiting a thickness of each layer of the stomach wall that ranged between the minimum and maximum values. In the small intestine, all histomorphometric parameters exhibited values that remained within the normal range. The conclusion of this research is combination did not result in an increase in gastric acid secretion and did not induce irritation to the gastric and small intestinal mucosa of mice.
EKSPLORASI GULA SIWALAN PADA PEMBUATAN SIRUP JAMU ANAK MELALUI PEMBERDAYAAN PEREMPUAN DESA LEMBUNG TIMUR KABUPATEN SUMENEP Inayah, Nurul; Mutiati, Enni; Hidayatullah, Mohammad Nurul
Jurnal Abdi Insani Vol 13 No 4 (2026): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v13i4.3399

Abstract

Lembung Timur Village is an area surrounded by siwalan trees, which are known for their brown sugar production. Brown sugar is a natural sweetener with a low glycemic index. Therefore, it is suitable for use as an ingredient in food and beverages as a substitute for cane sugar. Jamu herbal medicine tends to be unpopular with children due to its distinctive taste and smell. Therefore, it is necessary to explore the use of palm sugar in making herbal medicine for children to reduce the bitter taste. This community service activity involved 23 participants, namely mothers from the Lembung Timur Village PKK. The purpose of implementing Community Service (PKM) is to improve the quality and quantity of low-calorie palm sugar processing as an ingredient in herbal medicine that meets standardized quality standards. The implementation method consisted of preparation, counseling, training in making herbal syrup for children with the addition of palm sugar, TTG provision, and evaluation. The results of this community partnership program were 1) an increase in the partners' knowledge in making palm sugar herbal syrup. The enthusiasm of the participants was reflected in their active participation in asking questions and expressing their opinions. The evaluation showed a 50%-80% increase in knowledge compared to before the PKM activity. In addition, the tabulation of data on the partners' satisfaction with all PKM activities was above 50%. 2) An increase in the quantity of products produced, namely with the provision of TTG, which was able to produce 8-15 liters per cycle, or the equivalent of 32-60 bottles of ready-to-consume syrup. Through this program, it is hoped that the welfare of the community will improve through health and economic independence and that it will contribute to the progress of the village.