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Pengaruh Hemodialisis Terhadap Kejadian Kurang Pendengaran Sensorineural Pada Penderita Gagal Ginjal Kronik Ulfa, Loriana; Muyassaroh, Muyassaroh; Naftali, Zulfikar; Arwanto, Arwedi; Murbani, Ita
Majalah Kedokteran Bandung Vol 48, No 2 (2016)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (153.479 KB) | DOI: 10.15395/mkb.v48n2.763

Abstract

Kurang pendengaran sensorineural (KPSN) dapat terjadi pada kasus gagal ginjal kronik (GGK) yang dilakukan hemodialisis (HD). KPSN akibat HD terjadi pada berbagai frekuensi. Faktor yang diduga dapat mempengaruhi adalah usia, hipertensi, diabetes melitus (DM). Menganalisis pengaruh HD terhadap kejadian KPSN pada penderita GGK. Penelitian kohort pada 52 penderita GGK yang memenuhi kriteria inklusi. Terdiri dari dua kelompok, 26 penderita GGK mendapat HD dan 26 penderita GGK tanpa HD. Kelompok HD dilakukan pemeriksaan timpanoaudiometri sebelum HD dan setelah HD III. Kelompok tanpa HD dilakukan pemeriksaan timpanoaudiometri dalam waktu yang sama. Data usia, hipertensi, DM diperoleh dari rekam medik. Analisis menggunakan uji Chi-Square. Didapatkan 30,7% penderita KPSN dari kelompok HD, terdiri dari 26,9% derajat ringan dan 3,8% derajat sedang. Rerata penurunan NAP adalah 8,13 ± 5,30. Tidak didapatkan KPSN pada kelompok tanpa HD. Hemodialisis berpengaruh terhadap kejadian KPSN (p=0,004). Usia (p=0,084), hipertensi (p=0,215), DM (p=0,683) tidak berpengaruh terhadap kejadian KPSN. Hemodialisis berpengaruh terhadap kejadian KPSN. Usia, hipertensi dan DM tidak berpengaruh terhadap kejadian KPSN. [MKB. 2016;48(2):98–104]Kata kunci: Gagal ginjal kronik, hemodialisis, kurang pendengaran sensorineuralThe Effect of Hemodialysis on Sensorineural Hearing Loss in  Chronic Renal Failure PatientsSensorineural hearing loss (SNHL) can occur in patients with chronic renal failure (CRF) who receive hemodialysis (HD). SNHL is caused by hemodialysis occured in any frequency. The factors affect the disease include, among others, age, hypertension, and diabetes mellitus (DM).  The effect of hemodialysis onSNHL occurrence in patients with CRF was analyzed in this study. It was a cohort study on 52 patients with CRF who met the inclusion criteria. The sample consists of two groups, 26 patients with CRF who received hemodialysis and 26 patients with CRF who did not received hemodialysis. Timpanoaudiometry was performed on HD group (patient with HD) before the first HD and after the third HD. Timpanoaudiometry was performed on HD group (patient without HD) at the same time with  the first group. The data on age, hypertension, and DM were obtained from medical records. The results were analyzed with Chi-Square test. It was found that 30.7% SNHL patients  were in the HD group consisting of 26.9% mild degree patients and 3.8% moderate degree patients . Mean of hearing threshold decrease was 8.13 ± 5.30. There was no patient with SNHL in the non-HD group. Analysis with Chi-Square test showed that hemodialysis affects SNHL occurrence (p=0.004). Age (p=0.084), hypertension (p=0.215), and DM (p=0.683) do not affect SNHL occurrence. It is concluded that hemodialysis affects SNHL occurrence whileage, hypertension, and DM do not. [MKB. 2016;48(2):98–104]Key words: Chronic renal failure, hemodialysis, sensorineural hearing loss
Therapeutic potential of thymoquinone in regulating p63, claudin, and periostin in chronic rhinosinusitis with nasal polyps: An animal model study Ulfa, Loriana; Munir, Delfitri; Rambe, Andrina YM.; Farhat, Farhat; Wardani, Retno S.; Amin, Mustafa M.; Zahara, Devira; Ardinata, Dedi
Narra J Vol. 5 No. 1 (2025): April 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i1.1728

Abstract

High recurrence rate and the necessity for repeated surgical interventions contribute to the chronicity and treatment-resistant nature of chronic rhinosinusitis with nasal polyps (CRSwNP). Thymoquinone, known for its protective effects on epithelial integrity, has not been previously explored in CRSwNP. The aim of this study was to investigate the therapeutic potential of thymoquinone to restore epithelial integrity by assessing p63 transcription factor and claudin protein expressions, as well as periostin mRNA expression in an animal model. An in vivo study using post-test-only control group design was conducted in which male Wistar rats were randomly assigned to three groups, each consisting of 10 animals: healthy group, CRSwNP group, and thymoquinone-treated group for three weeks. Immunohistochemistry was used to analyze the p63 and claudin protein expressions, while periostin mRNA expression was quantified using quantitative reverse transcription polymerase chain reaction (qRT-PCR). This study found that thymoquinone significantly reduced p63 transcription factor expression compared to the untreated CRSwNP group (p=0.009). Claudin protein expression was significantly higher in thymoquinone-treated group compared to CRSwNP group (p=0.007), indicating improved epithelial barrier function. Periostin mRNA expression showed no significant difference between healthy and thymoquinone-treated groups (p=0.564), but a significant decrease was observed in CRSwNP group compared to thymoquinone-treated group (p=0.000) and between the healthy and CRSwNP groups (p=0.002), suggesting attenuation of tissue remodeling and inflammation. In conclusion, thymoquinone could enhance sinonasal epithelial barrier integrity in CRSwNP by downregulating p63 transcription factor, upregulating claudin protein expression, and reducing periostin mRNA expression. These findings emphasize the potential of thymoquinone as a therapeutic agent to mitigate inflammation and tissue remodeling in CRSwNP, warranting further investigation as a novel treatment option.