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All Journal PROSIDING SEMINAR KIMIA Science and Technology Indonesia JURNAL ATOMIK Jurnal Abdimas Mahakam
Ruga, Ritbey
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Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Energy Environmental Science Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Physics Public Health Social Sciences

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SKRINING AMILASE, LIPASE DAN PROTEASE DARI BAKTERI ENDOFIT DAUN CIPLUKAN (Physalis angulata L.) Nursanti, Oktavia Widya; Astuti, Winni; Ruga, Ritbey
JURNAL ATOMIK Vol 7 No 2 (2022)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

Endophytic bacteria is one of the source of extracellular enzymes producer. Enzymes that produced by endophytic bacteria are more beneficial because they can be cultured in small spaces with big quantities, relatively fast time and cheaper production costs compared to the sources of enzyme from plants and animals. This study was conducted to obtain amylase, lipase and protease from endophytic bacteria of ciplukan leaves (Physalis angulata L.). The research method used is experimental, by isolating endophytic bacteria from ciplukan leaves (Physalis angulata L.) in Nutrient Agar (NA) media and screening tests of extracellular enzyme activity from endophytic bacteria isolates. The isolation results was obtained 24 endophytic bacteria isolates that 4 bacteria isolates produce amylase, 3 bacteria isolates produce lipase and 2 bacteria produce protease.
UJI AKTIVITAS ANTIBAKTERI RIMPANG KUNYIT PUTIH (Kaempferia rotundaL.) TERHADAP Streptococcus sobrinus DAN Salmonella typhi -, Nurfadzillah; Marliana, Eva; Ruga, Ritbey
JURNAL ATOMIK Vol 6 No 2 (2021)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

An infectious disease caused by bacteria is a disease that often occurs in tropical areas. The used of plant extract is an interesting object of study to overcome these infectious diseases and because of an increase in bacterial resistance to antibiotics. White turmeric rhizome widely used by Indonesia society as an herb which has been used for the treatment of diarrhea and abdominal pain. This study aims to determine the content of secondary metabolities and inhibitory of white turmeric rhizome (Kaempferia rotunda L.) against Streptococcus sobrinus KCCM 11898 and Salmonella typhi ATCC 422 bacteria. The results of phytochemical screening white turmeric rhizome contained compound triterpenoids, steroids, phenolics, and saponins. An antibacterial activity test used agar diffusion method against Streptococcus sobrinus KCCM 11898 and Salmonella typhi ATCC 422 bacteria. Antibacterial activity white turmeric rhizome (Kaempferia rotunda L.) with concentration 1, 2, 4, 8% have an inhibition zone diameter against Streptococcus sobrinus KCCM 11898 bacteria is 10.33, 10.67, 17 and 25.17 mm respectively with the antibacterial strength is strong, meanwhile in Salmonella typhi ATCC 422 bacteria have an inhibition zone diameter was 8, 8.3, 11.83 and 13 mm respectively with the antibacterial strength is intermediate. Keywords: Antibacterial, White Turmeric Rhizome (Kaempferia rotunda L.), Agar Difussion
POTENSI AKTIVITAS ANTIOKSIDAN EKSTRAK METANOL BUNGA ASOKA (Ixora coccinea L.) Ramadhan, Aulia; Saleh, Chairul; Hairani, Rita; Ruga, Ritbey
JURNAL ATOMIK Vol 9 No 1 (2024)
Publisher : Jurusan Kimia FMIPA UNMUL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/ja.v9i1.1273

Abstract

Extraction of asoka (Ixora coccinea L.) flowers, phytochemical screening, and antioxidant activity of methanol extract of asoka flowers have been conducted. This study aimed to determine the content of secondary metabolites in the methanol extract of asoka flowers and its antioxidant activity using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method. The phytochemical screening test revealed that the methanol extract of asoka flowers contained secondary metabolites such as flavonoids, alkaloids, triterpenoids and saponins. With 1 mg/mL, the methanol extract exhibited potential as an antioxidant with an inhibition percentage of 88.10% while vitamin C as a standard used in this study at 1 mg/mL showed an antioxidant activity of 99.23%.
SKRINING BAKTERI ENDOFIT PENGHASIL AMILASE, LIPASE DAN PROTEASE DARI DAUN Syzygium myrtifolium Rahmaniah, Rahmaniah; Astuti, Winni; Ruga, Ritbey
JURNAL ATOMIK Vol 7 No 2 (2022)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

Endophytic bacteria are microorganisms that throughout or part of their life cycle inside plants and will not appear certain symptoms on the plant itself. Endophytic bacteria can produce enzymes. Enzymes produced by bacterial endophytes are more beneficial because the production time is faster. One of the plants that contain bacteria is Pucuk Merah (Syzygium myrtifolium). This study aims to isolate endophytic bacteria from leaves of Pucuk Merah (Syzygium myrtifolium) and to screen these endophytic bacteria as amylase, lipase and protease producers. The results of this study were obtained there are 24 isolates of endophytic bacteria from Pucuk Merah leaves. A total of 19 bacterial isolates from 24 bacterial isolates produce amylase, 1 isolate produce lipase and 24 isolates produce protease. Keywords: Endophytic bacteria, Syzygium myrtifolium, Amylase, Lipase, Protease
PENGHAMBATAN AKTIVITAS α-GLUKOSIDASE DARI EKSTRAK METANOL BUNGA BUGENVIL (Bougainvillea glabra Choisy) Puspitasari, Intan Dessy; Saleh, Chairul; Hairani, Rita; Ruga, Ritbey
JURNAL ATOMIK Vol 9 No 1 (2024)
Publisher : Jurusan Kimia FMIPA UNMUL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/ja.v9i1.1276

Abstract

The test of Phytochemical screening and antidiabetic activity of methanol extract of bugenvil (Bougainvillea glabra Choisy) flower against α-glucosidase inhibition have been conducted. This study aims to determine the content of secondary metabolite compounds and the effectiveness of the methanol extract of bugenvil flower against α-glucosidase activity. Phytochemical test showed that the methanol extract contained secondary metabolite compounds namely alkaloids, flavonoids, steroids, phenolics and saponins. By 50 µg/mL, the methanol extract of bugenvil flower can inhibit α-glucosidase activity with a percent inhibition value of 79.42% while acarbose as a positive control in this study exhibited antidiabetic activity against α-glucosidase of 99.30% at a concentration of 0.00006 µg/mL.
Strategi Peningkatan Kualitas Pembelajaran Kimia bagi Siswa SMA Negeri 1 Muara Badak melalui Praktikum Kimia Hairani, Rita; Koesnarpadi, Soerja; Wirawan, Teguh; Ruga, Ritbey; Ersanto, Tri Edi
Jurnal Abdimas Mahakam Vol. 8 No. 02 (2024): Juli
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24903/jam.v8i02.2595

Abstract

Tersedianya sarana dan prasarana di tingkat sekolah menengah atas (SMA) untuk melaksanakan praktikum kimia berupa laboratorium serta alat dan bahan praktikum kimia sangatlah penting terhadap peningkatan kualitas pembelajaran bagi siswa. SMA Negeri 1 Muara Badak merupakan salah satu SMA yang berada di kecamatan Muara Badak Kalimantan Timur dan memiliki sarana laboratorium untuk melakukan praktikum. Namun kegiatan praktikum kimia terkendala untuk rutin dilaksanakan yang dikarenakan kurang memadainya alat dan bahan praktikum di sekolah tersebut. Sehubungan dengan kendala yang dihadapi dan sebagai upaya implementasi salah satu poin Tri Dharma Perguruan Tinggi, yaitu pengabdian kepada masyarakat, maka tim dosen Jurusan Kimia FMIPA Universitas Mulawarman pada 5 Juni 2023 berinisiatif untuk melakukan pendampingan praktikum kimia dan juga menyediakan alat dan bahan praktikum kimia yang dapat dilaksanakan di sekolah tersebut. Kegiatan ini juga diparalelkan dengan penyampaian materi mengenai keselamatan dan kesehatan kerja (K3) di laboratorium oleh pihak PT. Global Environment Laboratory (PT. GEL) Samarinda yang merupakan mitra kerjasama. Pada kegiatan ini, pengumpulan data menggunakan instrument berupa angket pre-test dan post-test yang disebarkan ke 30 siswa SMA Negeri 1 Muara Badak. Hasil analisis terhadap jawaban angket pre-test menunjukkan bahwa sekitar 71% siswa setuju bahwa kimia adalah subjek yang menarik dan 72% siswa setuju bahwa kimia berguna untuk memahami fenomena sehari-hari. Selanjutnya, berdasarkan analisis data angket post-test menunjukkan bahwa sebagian besar siswa setuju dengan uraian yang dijabarkan di angket. Secara keseluruhan hasil analisis angket-angket tersebut menunjukkan bahwa kegiatan yang telah dilakukan memiliki dampak yang cukup baik bagi siswa SMA Negeri 1 Muara Badak dalam hal motivasi belajar terhadap pembelajaran kimia melalui metode praktikum.
SKRINING FITOKIMIA DAN UJI TOKSISITAS MENGGUNAKAN BRINE SHRIMP LETHALITY TEST (BSLT) DARI EKSTRAK DIKLOROMETANA RIMPANG TEMU KUNCI (Boesenbergia rotunda) Jonathan, Jonathan; Ruga, Ritbey; Hairani, Rita
JURNAL ATOMIK Vol 9 No 2 (2024)
Publisher : Jurusan Kimia FMIPA UNMUL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/ja.v9i2.1407

Abstract

The rhizome of Boesenbergia rotunda (temu kunci) is one of medicinal plants having potential for medicinal, supplementary food and drink due to its biological activities. The aims of this research are to identified the secondary metabolite compounds present in the dichlorometane extract of finger-root rhizomes through phytochemical screening using thin layer chromatography (TLC) method and to investigated its toxicity against Artemia salina L. shrimp larvae by using Brine Shrime Lethality Test (BSLT). The result of phytochemical analysis showed that the dichloromethane extract contains secondary metabolite compounds including flavonoids, phenolics and triterpenoids while the toxicity test of the extract showed its LC50 value of 303.68 µg/mL. It was indicated that the extract could be classified as toxic.
POTENSI AKTIVITAS ANTIBAKTERI EKSTRAK DIKLOROMETANA DAN METANOL BUNGA TEROMPET EMAS (Allamanda cathartica L.) TERHADAP BAKTERI Staphylococcus aureus ATCC 25923 Ayu, Linda; Kuspradini, Harlinda; Ruga, Ritbey
JURNAL ATOMIK Vol 9 No 2 (2024)
Publisher : Jurusan Kimia FMIPA UNMUL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/ja.v9i2.1357

Abstract

Potential antibacterial activity of dichloromethane and methanol extracts of the golden trumpet flower (A.cathartica L.) against S. aureus ATCC 25923 has been conducted. This study aimed to determine the values of thedichloromethane and methanol extracts of A. cathartica L. The antibacterial activity test was conducted using agardiffusion method and carried out in triplicate. The obtained results from the dichloromethane extract and methanol extractof the golden trumpet flower (A. cathartica L.) showed antibacterial activity with inhibition zone diameters of 9.66 mmfor dichloromethane extracts and 8.66 mm for methanol extracts against S. aureus ATCC 25923 bacteria.
Macaranga hullettii King ex Hook.f : SOURCE OF FLAVONOIDS Pramana, Aditiya Hadi Ryan; Marliana, Eva; Ruga, Ritbey
JURNAL ATOMIK Vol 5 No 1 (2020)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

Flavonoid compound had been isolated and characterized on fraction ethyl acetate of Macaranga hullettii King ex Hook.f leaves. The separation process in fraction ethyl acetate was performed using vacuum and gravity column chromatography with a gradient polarity method. The isolate was characterized as MH-1 flavonoid compound in the yellow crystals and 0.0432 g. Analysis of MH-1 with spectrophotometer UV showed two peaks at λ 268.46 (band II) and 340 nm (band I). Analysis of MH-1 with spectrophotometer IR showed OH functional groups, C-O-C, C=O, aliphatic C-H, aromatic C-H, and aromatic C=C. The MH-1 is assumed to be a flavonoid.
ENZYMATIC HYDROLYSIS WITH Saccharomyces cerevisiae FOR ETHANOL PRODUCTION FROM MULI BANANA PEEL (Musa acuminata Linn) Julianto, Faisal Reja; Kartika, Rudi; Ruga, Ritbey
JURNAL ATOMIK Vol 4 No 2 (2019)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

Ethanol production from starch of muli banana peel by enzymatic hydrolysis with the addition of gelatin as a fermentation nutrient for Saccharomyces cerevisiae was done. This research consists of hydrolysis, fermentation, distillation and testing carried out by Gas Chromatography. This research was conducted to determine the optimal time and the optimum nutrient concentration, that can be seen that the optimum time at 7 days and the optimum nutrient concenctration in addition of 2% nutrient with ethanol concentration of 36.344%.
Co-Authors -, Nurfadzillah Ayu, Linda Chairul Saleh Daniel Djihan Ryn Pratiwi, Djihan Ryn Ersanto, Tri Edi Eva Marliana, Eva Hairani, Rita Indra Masmur Jonathan Jonathan Julianto, Faisal Reja Magdaleni, Agustina Rahayu Masmur, Hestina Matutu, Deni Ari Nursanti, Oktavia Widya Pramana, Aditiya Hadi Ryan Puspitasari, Intan Dessy Rahmaniah, Rahmaniah Ramadhan, Aulia Ritson Purba, Ritson Rudi Kartika, Rudi Saibun Sitorus Soerja Koesnarpadi Subur P Pasaribu Supratman, Rafif Ghanaifalah Dely Teguh Wirawan, Teguh Winni Astuti, Winni