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All Journal E-Jurnal Medika Udayana Biology, Medicine, & Natural Product Chemistry BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains Jurnal Ners Jurnal Bali Membangun Bali Prominentia Medical Journal Indonesian Journal of Pharmacology and Therapy
Udiyani, Desak Putu Citra
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EKSTRAK ETANOL UMBI UBI JALAR UNGU (Ipomoea batatas L) MENINGKATKAN KETEBALAN ENDOMETRIUM Udiyani, Desak Putu Citra; Jawi, , I Made; Sugiritama, Wayan; Nyoman Arijana, I Gusti Kamasan; Sumadewi, Komang Trisna
E-Jurnal Medika Udayana Vol 12 No 9 (2023): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2023.V12.i09.P11

Abstract

Menopause merupakan salah satu proses yang alami untuk Wanita, dengan terapi Hormone Replacement Therapy (HRT) menimbulkan efek endometriosis dan tromboemboli vena. Umbi ubi jalar ungu (Ipomoea batatas L) merupakan tumbuhan dengan antosianin yang mampu meningkatkan ploriferasi uterus. Tujuan penelitian: penelitian ini membuktikan ekstrak etanol umbi ubi jalar ungu (Ipomoea batatas L) mampu meningkatkan ketebalan endometrium uterus. Metoda penelitian: rancangan penelitian True Experimental-Post Test Only Control Group Design dimana 28 ekor tikus Wistar betina dikelompokkan menjadi 4 kelompok (@7 ekor tikus): P0 merupakan kelompok kontrol, P1 dengan esktrak dosis 10 mg/kgBB, P2 dengan dosis 30 mg/kgBB, dan P3 dosis 100 mg/kgBB diberikan selama 30 hari melalui sonde, pengangkatan uterus pada hari ke 30 dan mengamati ketebalan endometrium uterus. Hasil penelitian: ketebalan endometrium uterus antar kelompok perlakuan dengan rerata berbeda secara signifikan (p=0,000). Kelompok P3 dengan ketebalan endometrium paling tinggi. Kesimpulan: disimpulkan bahwa ekstrak etanol umbi ubi jalar ungu mampu meningkatkan ketebalan endometrium uterus tikus putih yang diovariektomi. Kata kunci: Ekstrak, ubi jalar ungu, antosianin, uterus
Phytochemical Screening and Antioxidant Activity Analysis of N-Hexane Extract of Sonneratia alba Mangrove Leaves Surya, Putu Rissa Almadea; Wijaya, Made Dharmesti; Udiyani, Desak Putu Citra
Biology, Medicine, & Natural Product Chemistry Vol 12, No 2 (2023)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2023.122.631-635

Abstract

Mangrove plants have been widely used by people in various regions of Indonesia as traditional medicine for the treatment of wounds, fever, diarrhea, and others. Extreme habitat in coastal areas with high salinity and low oxygen level makes mangrove plants produce a variety of unique secondary metabolites and are rich in antioxidants. Although there have been studies on phytochemicals and antioxidant activity conducted, the study of mangroves in Bali, Indonesia, has not been widely explored. This study aims to determine the compounds contained in mangrove leaf extract and their antioxidant activity. The species to be studied is Sonneratia alba, which is commonly found in the Ngurah Rai Mangrove Forest, Bali, Indonesia. In this experimental study, compound extraction was carried out by maceration method using n-hexane solvent. Phytochemical screening using qualitative methods and GC-MS was carried out to determine the phytochemical compounds in the extract, while 1,1-diphenyl-2-picrylhydrazyl (DPPH) method was used to determine antioxidant activity. The result showed that the n-hexane S. alba mangrove leaves extract display strong antioxidant activity with IC50 value of 64.432 ± 7.675 ppm. The qualitative phytochemical tests showed that the extract contained phenol and steroid, which are known to have antioxidant properties. Moreover, GC-MS analysis showed that there are two compounds with the largest %area namely gamma-sitosterol and dl-alpha-tocopherol (52.88% and 7.77%, respectively), which have been reported to have antioxidant activities. To conclude, these findings demonstrate that n-hexane extract of S. alba mangrove leaves from Ngurah Rai Mangrove Forest, Bali, to have potential antioxidant activity.
Perbandingan Daya Ledak Otot Tungkai Antara Rasio Panjang Tungkai Atas dan Bawah > 1 dan < 1 Panditama, Yukta; Subrata, Tanjung; Udiyani, Desak Putu Citra
Bahasa Indonesia Vol 5 No 1 (2024): Prominentia Medical Journal
Publisher : Universitas Ciputra Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37715/pmj.v5i1.4320

Abstract

Muscle explosive power is a person's ability to perform explosive activities suddenly and quickly by exerting all the strength in a short time. The explosive power of the leg muscles is influenced by the length of the leg. The purpose of this study was to find out the comparison of leg muscle explosive power between ratio of upper and lower leg length more than 1 and less than 1. The method of this study was using an analytic observational design with a cross-sectional approach. This study was carried out on 50 male Warmadewa University students who participate in basketball extracurriculars. The study data was analyzed by SPSS program. The results were showed as follow: the average of leg muscle explosive power in all subject and in subjects with a leg length ratio more than 1 and less than 1 were 21,62 ± 3,43 cm; 22,02 ± 3,51 cm; and 19,14 ± 1,34 cm respectively. The conclusion could be drawn that the comparison was significant. This study suggests that the selection of basketball players/athletes should be choosen palyers/athletes with an upper and lower leg length ratio of more than 1.
Antibiotic use at the ear, nose, and throat outpatient clinic in Bangli Regional Hospital, Bali Sudiadewi, Ni Putu Eka; Permatananda, Pande Ayu Naya Kasih; Udiyani, Desak Putu Citra; Cahyawati, Putu Nita; Aryastuti, Sri Agung
Indonesian Journal of Pharmacology and Therapy Vol 6 No 2 (2025)
Publisher : Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada and Indonesian Pharmacologist Association or Ikatan Farmakologi Indonesia (IKAFARI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijpther.20992

Abstract

Ear, nose, and throat (ENT) diseases are defined as diseases that occur around the ear, nose, and throat organs, which do not recognize seasons and can affect various ages. The prevalence of ENT diseases in Indonesia is quite high, with the most common therapy being antibiotics. Irrational antibiotics use can lead to increased treatment costs, risk of side effects, and toxicity. This study aimed to evaluate the use of antibiotics among ENT outpatient. This was a cross-sectional study using medical records data from the ENT Outpatient Clinic in Bangli Regional Hospital in 2022 that meet the inclusion criteria. Data were analyzed descriptively and presented in tables or diagrams. A total of 80 samples were evaluated, predominantly aged 18-44 yo (60%), female (53.8%), and diagnosed with chronic suppurative otitis media (20%). The pattern of antibiotic prescription consisted of monotherapy antibiotics (62.5%) in solid dosage forms (58.8%), administered orally (60%), and with a duration of administration ≥ 5 d (82.5%). Patients aged 18-44 yr, both male and female, receiving either monotherapy orpolytherapy antibiotics were predominantly cases of infection (>90%). The results of this study are expected to serve as one of the sources of information for the development of practical clinical guidelines for ENT patients receiving antibiotic therapy, particularly at the Bangli Regional Hospital, Bali.
Phytochemicals and Antibacterial Activity of Impatiens balsamina L. Leaf Extracts Against Gram-Positive and Gram-Negative Bacteria Fridayanthi, Putu Utari; Wijaya, Made Dharmesti; Udiyani, Desak Putu Citra; Indraningrat, Anak Agung Gede; Setiabudy, Marta
Biology, Medicine, & Natural Product Chemistry Vol 14, No 1 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.141.327-335

Abstract

The emergence of antibiotic resistance as one of the global public health threats makes research on new antibacterial compounds urgently needed. Among natural resources, the Impatiens balsamina plant has the potential to be explored as the new source of antibacterial agents. This study aimed to identify the phytochemical composition and evaluate the antibacterial activity of I. balsamina leaf extracts against Gram-positive and Gram-negative bacteria. Extracts were prepared using the maceration method with methanol, chloroform, and n-hexane solvents at a 1:5 sample-to-solvent ratio. Phytochemical screening was performed qualitatively, and antibacterial activity was evaluated using the disc diffusion assay. Analysis of methanol extract detected the presence of flavonoids, saponins, tannins, phenols, steroids, and glycosides, while chloroform extract consisted of tannins, phenols, and steroids. On the other hand, steroids were the only compounds detected qualitatively in n-hexane extract. Antibacterial testing revealed that methanol extract exhibited the highest activity, with zones of inhibition (ZOI) of 15.10±0.18 mm, 9.40±0.30 mm, 14.75±1.28 mm, and 8.67±0.50 mm against Streptococcus mutans FNCC 0405, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, and Klebsiella pneumoniae ATCC 700603, respectively. A concentration-dependent ZOI was observed in the methanol extract, with activity increasing at higher concentrations. GC-MS analysis of the methanol extract identified 32 compounds, including n-hexadecanoic acid (12.12%), 2-acetylbenzoic acid (8.26%), 5-hydroxymethylfurfural (8.21%), and 2-methoxy-4-vinylphenol (4.67%), which are known to possess antimicrobial, antioxidant, and anti-inflammatory activities. Chloroform extract showed moderate activity against S. mutans (7.04±0.15 mm) and S. aureus (7.10±0.31 mm), while n-hexane extract exhibited no antibacterial activity. The significant antibacterial activity of methanol extract is likely due to its rich phytochemical composition, highlighting methanol as an effective solvent for extracting bioactive compounds. These findings provide a strong foundation for further exploration of I. balsamina leaf extracts as a source of antibacterial agents.
Aktivitas Antioksidan pada Ekstrak Limbah Kulit, Biji, dan Ampas Jeruk Siam Kintamani (Citrus nobilis et reticulata) dengan Pelarut Metanol Dewi, Ni Kadek Novia Puspita; Permatananda, Pande Ayu Naya Kasih; Udiyani, Desak Putu Citra; Nita Cahyawati, Putu; Wayan Erly Sintya Dewi, Ni
BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains Vol. 8 No. 5 (2025): Bioedusains: Jurnal Pendidikan Biologi dan Sains
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31539/2erqy756

Abstract

This study aims to analyze the antioxidant activity of waste extracts from the peel, seeds, and pulp of Kintamani tangerines and to implement a practical approach to by-product management based on a zero-waste system. The method used is a pure experimental study with a post-test only design. Extraction was carried out through repeated maceration using methanol as a solvent. Antioxidant activity was tested using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method and analyzed with a UV-Vis spectrophotometer at a wavelength of 517 nm. The results showed that the peel extract of Kintamani tangerines could fade the purple color of the DPPH solution, whereas the seed and pulp extracts did not show a significant change. The IC50 values were 136.17 ppm for the peel extract, 6727.86 ppm for the seed extract, and 497.57 ppm for the pulp extract. In conclusion, the peel extract of Kintamani  tangerines exhibited the highest antioxidant activity among the three extracts, categorized as moderate based on Blois' classification.   Keywords: Antioxidant Activity, Pulp, Kintamani Tangerine, Seed, DPPH, Peel
Phytochemical and Antioxidant Capacity Test on Turmeric Extract (Curcuma Longa) Traditionally Processed in Bali Permatananda, Pande Ayu Naya Kasih; Aryastuti, Anak Agung Sri Agung; Cahyawati, Putu Nita; Udiyani, Desak Putu Citra; Wijaya, D.; Pandit, I Gde Suranaya; Wirajaya, Anak Agung Ngurah Mayun
Bali Membangun Bali: Jurnal Bappeda Litbang Vol 1 No 2 (2020): August 2020
Publisher : Badan Riset dan Inovasi Daerah Provinsi Bali

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51172/jbmb.v1i2.115

Abstract

Purpose: The purpose of this study was to determine the ways and objectives of the use of turmeric by Balinese, as well as the phytochemical content and antioxidant capacity of turmeric extract which is traditionally processed in Bali. Research methods: The method and purpose of utilizing turmeric were obtained through observation and interviews with 900 Balinese respondents. Quantitative phytochemical tests include starch, protein, flavonoid, tannin, phenol and vitamin C levels and qualitatively for the presence of triterpenes, steroids, alkaloids, and saponins. Antioxidant capacity was measured using the DPPH method. Findings: Through this research, we found there were only 36.8% of respondents had ever used turmeric as a traditional medicine. Utilization of turmeric was mostly in the form of loloh or traditional drinks. Phytochemical test results showed turmeric extract had 67.38% starch, 3.42% protein, 2709.39 mg/100 gr flavonoids, tannins 291.64 mg/100gr, phenol 1584.04 mg/100 gr, and vitamin C 0.06 mg/100gr. Qualitatively, turmeric extract contained triterpenes, alkaloids, and saponins, but did not contain steroids. The antioxidant capacity of turmeric extract was 70.9 mg/L GAEAC. Implications: Turmeric extract is a traditional medicine made from nature that is most commonly used by Balinese and very potential to be developed as an antibacterial, antioxidant, anti-inflammatory, or other benefits that still need further investigation.
Aktivitas Antioksidan Ekstrak Limbah Kulit Jeruk Siam Kintamani (Citrus nobilis) dengan Pelarut Polar, Semipolar, dan Nonpolar Nirmalasari, Ni Kadek Diah Ayu; Permatananda, Pande Ayu Naya Kasih; Udiyani, Desak Putu Citra; Aryastuti, Anak Agung Sri Agung; Dewi, Erly Sintya
Jurnal Ners Vol. 8 No. 1 (2024): APRIL 2024
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jn.v8i1.19385

Abstract

Jeruk memiliki banyak komponen nutrisi yang berperan sebagai sumber antioksidan alami. Namun, sebagian besar nutrisi tersebut terdapat pada kulit jeruk yang jarang dimanfaatkan. Kulit Jeruk Siam mengandung senyawa flavonoid, alkaloid, vitamin C, likopene, pektin, serta tannin, sehingga memiliki potensi tinggi untuk dikembangkan. Penelitian ini bertujuan untuk menganalisis aktivitas antioksidan pada limbah kulit jeruk siam kintamani dengan berbagai jenis pelarut. Metode ekstraksi yang digunakan adalah maserasi, dan pengujian aktivitas antioksidan dilakukan melalui metode Diphenylpicrylhydrazil (DPPH). Hasil dari penelitian ini adalah pelarut metanol berhasil mengubah larutan DPPH yang memiliki warna ungu menjadi berwarna kuning, selain itu pengujian dengan pelarut etil asetat juga mampu mengubah larutan DPPH menjadi warna kuning atau bening. Namun perubahan warna dari ungu ke kuning tidak tampak signifikan pada pelarut n-hexana. Nilai IC50 untuk pelarut metanol sebesar 136,17 atau 0,13617 mg/ml, etil asetat sebesar 1412,05 atau 1,41205 mg/ml dan n-hexana sebesar 1736,71 atau 1,73671 ml/mg. Apabila ketiganya dibandingkan maka dapat disimpulkan pelarut metanol memiliki aktivitas antioksidan tertinggi dibandingkan dengan pelarut lainnya.
Co-Authors Anak Agung Sri Agung Aryastuti Cahyawati, Putu Nita Dewi, Erly Sintya Dewi, Ni Kadek Novia Puspita Fridayanthi, Putu Utari I Gde Suranaya Pandit I Gusti Kamasan Nyoman Arijana, I Gusti Kamasan Indraningrat, Anak Agung Gede Jawi, , I Made Made Dharmesti Wijaya Nirmalasari, Ni Kadek Diah Ayu Nita Cahyawati, Putu Pande Ayu Naya Kasih Permatananda Panditama, Yukta Setiabudy, Marta Sudiadewi, Ni Putu Eka Sugiritama, Wayan Sumadewi, Komang Trisna Surya, Putu Rissa Almadea Tanjung Subrata Wayan Erly Sintya Dewi, Ni Wijaya, D. Wirajaya, Anak Agung Ngurah Mayun