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POTENSI BIOINSEKTISIDA DARI EKSTRAK DAUN, KULIT BATANG DAN BIJI TUMBUHAN PANGI (PANGIUM EDULE REINW.) DALAM MENINGKATKAN MORTALITAS LARVA CROCIDOLOMIA BINOTALIS Manoppo, Jacklin Stella Salome; Sakul, Ernest Hanny; Tengker, Anita Constantin
FRONTIERS: JURNAL SAINS DAN TEKNOLOGI Vol 2, No 1 (2019): APRIL 2019
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (565.037 KB)

Abstract

Kandungan insektisida alami dari ekstrak daun, kulit batang dan biji pangi (Pangium edule Reinw.) untuk mengatasi larva Crocidolomia binotalis telah dilakukan di laboratorium. Penelitian ini telah dilakukan untuk mengetahui pengaruh penggunaan insektisida nabati untuk mengendalikan C.binotalis yang merupakan hama tanaman sawi. Penelitian ini bertujuan untuk menentukan konsentrasi ekstrak yang paling efektif dan konsentrasi ekstrak paling aktif; untuk mengevaluasi tingkatan konsentrasi ekstrak pada larva yang diujicobakan; dan untuk mengkarakterisasi kandungan fitokimia dari ekstrak daun, kulit batang dan biji pangi (Pangium edule Reinw.) Penelitian ini adalah penelitian eksperimen dan dimulai dengan tes skrining fitokimia untuk mendapatkan hasil metabolit sekunder di dalam ekstrak. Selanjutnya, diikuti dengan uji LC/LD50-48h terhadap larva instar III dari hama C.binotalis. Kemudian, ekstrak fraksi n-heksan dan etanol digunakan untuk perlakuan rancangan acak lengkap (RAL) dengan ANAVA dan diikuti dengan penghitungan nilai LD50-48h dengan menggunakan analisis probit. Hasil penelitian menunjukkan bahwa konsentrasi ekstrak biji pangi dengan menggunakan fraksi n-heksan lebih efektif dalam meningkatkan mortalitas larva dengan nilai LD50-48h = 11,25 mg/L. Sedangkan ekstrak kulit batang memiliki nilai LD50-48h = 30,20 mg/L dan nilai LD50-48h = 25,75 mg/L diperoleh dari ekstrak daun pangi. Tingkat kematian larva yang tertinggi diperoleh pada perlakuan dengan konsentrasi 50 ppm ekstrak biji fraksi n-heksan sebesar 93,5%, kemudian 86,3% untuk ekstrak daun pangi dan 75,5% untuk ekstrak kulit batang pangi. Ekstrak tumbuhan pangi dengan menggunakan fraksi etanol, ditemukan mengandung alkaloid, saponin, flavonoid, terpenoid, phenol dan tannin. Ekstrak biji pangi dengan menggunakan fraksi n-heksan merupakan insektisida nabati yang paling efektif dalam mengendalikan hama C.binotalis. Hal ini mengindikasikan bahwa insektisida botani dari ekstrak tumbuhan pangi memiliki potensi dalam mengendalikan hama C.binotalis pada tanaman sawi. Evaluasi dan karakterisasi komponen aktif dari ekstrak dan aplikasi di lapangan sangat diperlukan untuk penelitian selanjutnya.
Pemberdayaan Kelompok Tani Wanita Mandiri Dalam Meningkatkan Produksi Tanaman Cabai Varietas Nirmala-F1 Melalui Penerapan Teknologi MPHP dan BioBoost Sakul, Ernest Hanny; Tuerah, Philotheus Erwin Alex; Manoppo, Jacklin Stella Salome
VIVABIO: Jurnal Pengabdian Multidisiplin Vol 1, No 2 (2019): VIVABIO: Jurnal Pengabdian Multidisiplin
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/vivabio.1.2.2019.24930

Abstract

Teknologi Mulsa Plastik Hitam Perak (MPHP) dan BioBoost adalah teknologi yang ramah lingkungan dan mampu meningkatkan produktifitas lahan pertanian sehingga hasil pertanian akan meningkat baik mutu maupun jumlah hasil panennya. Pemanfaatan Bio-Boost sebagai pupuk organik yang mengandung mikroorganisme tanah yang unggul, diaplikasikan untuk meningkatkan kesuburan tanah sebagai hasil proses biokimia tanah dalam budidaya dan peningkatan produksi tanaman cabai varietas nirmala F-1. Hasil pengamatan dan survey di lapangan khususnya di Desa Kembuan Kecamatan Tondano Utara Kabupaten Minahasa banyak ditemukan lahan/ladang atau bekas sawah yang sudah tidak diefektifkan lagi oleh petani pemilik, sehingga menjadi lahan tidur dan hanya menjadi tempat menggembalakan hewan ternak seperti sapi dan kuda.  Selain itu juga, limbah organik yang dapat ditemukan di Desa Kembuan adalah limbah hasil peternakan ayam berupa kotoran ayam, yang bila tidak dikelola dengan baik akan berpotensi mencemari lingkungan. Potensi pemanfaatan lahan tidur dan limbah organik di pedesaan merupakan sebuah peluang usaha untuk meningkatkan kesejahteraan kelompok tani.  Berdasarkan hal tersebut di atas, maka dilaksanakanlah Program Kemitraan Masyarakat Kelompok Tani Wanita Mandiri Desa Kembuan Kecamatan Tondano Utara Kabupaten Minahasa Propinsi Sulawesi Utara. Kebutuhan aplikasi teknologi pertanian yang diperlukan oleh kelompok tani ini antara lain adalah teknologi Bio-Boost dan Effective Microorganism (EM4) sebagai agen hayati yang mempercepat proses dekomposisi limbah kotoran ayam menjadi pupuk organik berkualitas, dikombinasikan dengan teknologi mulsa plastik hitam perak dan pengendalian hama menggunakan bioinsektisida, ternyata mampu memberikan hasil yang baik dalam budidaya tanaman cabai rawit varietas nirmala F-1. Hasil panen perdana yang diperoleh kelompok tani ini yaitu cabai rawit varietas nirmala yang memiliki karakteristik morfologi buah lancip, dengan ukuran 4 x 0,8 cm, pedas, warna merah mengkilap dan dapat mulai dipanen pada umur 82-85 hari setelah tanam (HST), rata-rata berat buah cabai per tanaman adalah 0,75 – 1,0 kg per tanaman, dengan bobot per buah berkisar 2 – 3 gram dan memiliki potensi hasil 8 – 10 ton/ha.
The bioactive contents and antioxidant activity of honey bee nest extract of Apis dorsata Binghami from the North Sulawesi Mokosuli Yermia Semuel; Eva Sherly Nonke Kaunang; Jacklin Stella Manopo
Molekul Vol 14, No 2 (2019)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (411.303 KB) | DOI: 10.20884/1.jm.2019.14.2.502

Abstract

Apis dorsata Binghami is a Sulawesi endemic honey bee. Apis dorsata Binghami cannot be bred, so it still lives wildlyin the forests of Sulawesi. However, Apis dorsata Binghami produces more honey, compared to all honey bee species. Furthermore, the diversity of plants as a source of nectar, pollen and plant resin, which is used in the formation of nests and honey is more, than all types of honey bees in the world. Ethnomedically, the Minahasa community has long used honeynest for degenerative diseases such as hyperlipidemia and cancer. Nevertheless, there have been no research reports on bioactive content and bioactivity of Apis dorsataBinghami nest extract.  This research wasaimed to determinethe bioactive content of honey bee nest and to obtain the inhibitory concentration 50 (IC50) antioxidant activity of honey bee nest extractofApis dorsata Binghami. Honey bee nest was obtained directly from the forest of Minahasa peninsula, North Sulawesi, Indonesia. Extraction of fresh honey bee nest was conductedusingmaceration method. Bioactive content analysis was carried out by the Harborne method, followed by analysis using UV Vis spectrophotometer and High performance liquid chromatography. IC50antioxidant activity of honey bee nest extract was obtained using DPPH free radical reduction method. The results showed that Apis dorsata Binghami honey bee nest extract containedalkaloids, flavonoids, saponins, tannins, steroids and triterpenoids. Identified flavonoids displayed the highest phytochemical content. Based on the results of HPLC and UV Vis spectrophotometer analysis, there were 20 flavonoid derivatives found in honey bee nest samples in Minahasa. Ethanol extract and n-hexane extract showed high free radical reduction activity compared to vitamin C as a control treatment. However, ethanol extract produced the highest DPPH free radical reduction activity.
Penggunaan Pupuk Organik untuk Meningkatkan Hasil Tanaman Cabai Kelompok Tani di Kabupaten Minahasa Jacklin Stella Salome Manoppo; Ernest Hanny Sakul; Marlina Karundeng
DEDIKASI Vol 20, No 1 (2018): Jurnal Dedikasi
Publisher : Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26858/dedikasi.v20i1.7931

Abstract

After doing observations and surveys in Paslaten Village, Remboken District, it was found that manyfields or former rice fields which are no longer effective by the owner's farm. Therefore, the fields become idle landand have the potential to be no longer productive, and currently only become a place to bind livestock. Moreover,there are many wastes, especially organic waste as a result of the daily activities of community life in PaslatenVillage. One type of organic waste found is chicken manure, which if it is not reused, it will cause an environmentalpollution. Thus, it is better to manage the waste into organic fertilizer. Paslaten Village, Remboken District, thereare Masawangan and Pinaesaan farmer groups who have tried to plant chili plants in the traditional way according tothe local wisdom in their village. Nevertheless, the production of chili crops is still lacking. As a result, a training isneeded to be carried out in order to increase the production of chili crops. The necessity for the application ofagricultural technology that is required by this farmer groups is the use of effective microorganisms (EM4) asbiological agents that can accelerate the decomposition process of chicken manure into quality organic fertilizer. Itis combined with black silver plastic mulch technology and integrated pest control using bio-pesticides, with a targetto increase the production and quality of chili plants. The first crop yield which was obtained by the farmer groupswas Nirmala variety chili. It has sharp fruit, 4 x 0.8 cm, spicy, shiny red color and can be harvested at the age of 80-85 days after planting (DAP). The average weight of chili per plants are 0.5 - 1.0 kg per plant, with weights per fruitranging from 2-3 grams and potential crops are 8-12 tons/ ha.
POTENTIAL EXTRACTS OF PANGIUM EDULE REINW AND DERRIS ELLIPTICA WALLICH AS BOTANICAL MOLLUSCICIDES FOR MANAGEMENT OF GOLDEN APPLE SNAIL Pomacea canaliculata Lamarck Jacklin Stella Salome Manoppo
Agrotech Journal Vol 2, No 2 (2017): Agrotech Journal (ATJ)
Publisher : Universitas Sembilanbelas November Kolaka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31327/atj.v2i2.243

Abstract

The research purposed was compared of two extracts as molluscicidal activities from root of Derris elliptica Wallich. and Pangium edule Reinw seed, that assessed to 3-month old snails Pomacea canaliculata L. The Golden apple snails is widely regarded as worst invasive pest species in the rice growing area. It normally destroys the young stems and leaves paddy and could consume 7 – 24 rice seedlings per day. The experiment research started with the mortality test of the golden apple snail, meanwhile hexane extract fraction and etanol extract fraction have completely jumble mode; using the lethal concentration (LC) have mean to describe short term potency of poisonous (toxicity) from materials and can gave little effect or impact for environment; processing phytochemical test from n-hexane extract and etanol extract of root D.elliptica and P.edule showed positive to contain tanin, saponin and fenol. The data of LC50 from n-hexane fraction measure with probit analyze (9,905 mg L-1) by D.elliptica L. with high toxic category, and n-hexane fraction (11,574 mg L-1) by P.edule Reinw. with toxic category are more effective for golden apple snail control. The golden apple snail mortality was highest using 5000 ppm n-hexane fraction showed 93,3% from D.elliptica and using 5000 ppm n-hexane fraction from P.edule showed 63,3%. In conclusion, both of extracts from D.elliptica roots and P.edule seeds was showed potency as botanical mollusicicides and it can be apply in the field 
Entrepreneurial Development of C Pengembangan Wirausaha Cabai Rawit Varietas Bara F-1 Pada Kelompok Tani Wanita Berdikari Melalui Aplikasi Teknologi ULiBiCo, EM4 dan Plastikultur-MPHP : Entrepreneurial Development of Cayenne Pepper Variety Bara F-1 in the Berdikari Women Farmers Group through Technology Applications ULiBiCo, EM4 and MPHP Sakul, Ernest Hanny; Manoppo, Jacklin Stella Salome; Ngangi, Jantje; Rayer, Debby Jacqueline Jochebed; Nangoy, Wiesye Maya Selfia; Lawalata, Helen Joan; Rompas, Christny Ferdina Evie; Djarang, Rievo Handry; Sakul, Zefanya Escriva Rafael; Tengker, Anita Costanci Christine; Rawung, Stanny Sicilia; Silangen, Patricia Mardiana; Rumondor, Desire Atna Sridanty
Vivabio: Jurnal Pengabdian Multidisiplin Vol. 7 No. 2 (2025): VIVABIO: Jurnal Pengabdian Multidisiplin
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/vivabio.v7i2.63298

Abstract

Tonsealama is a village that has the potential to develop an environmentally friendly organic-based agribusiness farmer group business that has maximum agribusiness products and high economic value. The livelihood of the villagers is dominated by farmers and farm laborers and only a small part is engaged in services. The development of agribusiness entrepreneurship of raw chilli bara F1 variety for women's farmer groups in Keroit village is deemed necessary to be developed, in order to utilize idle land and organic household waste products. This is also a business opportunity to increase household economic income and the welfare of women's farmer groups and their members. Based on the above, the Community Partnership Program for Women Farmers Group in Tonsealama Village was implemented. This program includes the use of agricultural technology products in the form of ULiBiCo (Unima Liquid Biofertilizer Combination) and Effective Microorganism (EM4) applications that help the decomposition process of chicken manure waste into quality organic fertilizer, and combined with the use of silver black plastic mulch technology (MPHP) and bioinsecticide-based pest control, it turns out to be able to provide good yields in the cultivation of cayenne pepper plants of the bara F-1 variety. The morphological character of the first harvest obtained is the quality of the cayenne pepper fruit, which has a sharp fruit, with a length of ± 3 cm, a diameter of ± 0.4 cm, shiny red skin, spicy taste, and can be harvested at the age of 78 - 100 days after planting (HST), the average yield of this cayenne pepper is 0.85 - 1.0 kg per plant, with a weight per fruit ranging from 0.9 - 1.8 grams and has a potential yield of 8.9 - 10 tons / ha. ABSTRAK Tonsealama merupakan desa yang memiliki potensi untuk dikembangkannya sebuah usaha kelompok tani agribisnis berbasis organik ramah lingkungan yang memiliki produk hasil agribisnis yang maksimal dan bernilai ekonomi tinggi. Mata pencaharian penduduk desa masih didominasi oleh para petani. Pengembangan wirausaha agribisnis cabai rawit varietas bara F1 pada kelompok tani wanita di desa Tonsealama dirasa perlu untuk dikembangkan, guna memanfaatkan lahan tidur dan produk limbah organik rumah tangga. Hal ini juga merupakan sebuah peluang usaha untuk meningkatkan pendapatan ekonomi rumah tangga dan kesejahteraan kelompok tani wanita beserta anggotanya. Berdasarkan hal tersebut di atas, maka dilaksanakanlah Program Kemitraan Masyarakat Kelompok Tani Wanita di Desa Tonsealama. Program ini meliputi pemanfaatan produk teknologi pertanian berupa aplikasi ULiBiCo (Unima Liquid Biofertilizer Combination) dan Effective Microorganism (EM4) yang membantu proses dekomposisi limbah kotoran ayam menjadi pupuk organik berkualitas, dan dikombinasikan dengan pemanfaatan teknologi mulsa plastik hitam perak (MPHP) serta pengendalian hama berbasis bioinsektisida, ternyata mampu memberikan hasil panen yang baik dalam budidaya tanaman cabai rawit varietas bara F-1. Karakter morfologi hasil panen pertama yang diperoleh yaitu kualitas buah cabai rawit memiliki buah lancip, dengan panjang ± 3 cm, diameter ± 0,4 cm, kulit buah berwarna merah mengkilap, berasa pedas,  dan dapat dipanen pada umur 78 - 100 hari setelah tanam (HST), rata-rata hasil cabai rawit ini yaitu 0,85 - 1,0 kg per tanaman, dengan bobot per buah berkisar 0,9 – 1,8 gram dan memiliki potensi hasil 8,9 – 10 ton/ha.
The diversity and distribution of residential termites in the Minahasa Raya Region, North Sulawesi Province, Indonesia JACKLIN STELLA SALOME MANOPPO; RAMADHANI EKA PUTRA; RUDI DUNGANI; INTAN AHMAD
Biodiversitas Journal of Biological Diversity Vol. 25 No. 1 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250105

Abstract

Abstract. Manoppo JSS, Putra RE, Dungani R, Ahmad I. 2024. The diversity and distribution of residential termites in the Minahasa Raya Region, North Sulawesi Province, Indonesia. Biodiversitas 25: 39-48. Termites are major pests in urban settings, particularly due to their preference for wood, despite its ecological importance leading to structural damage to buildings. Studies showed a gap in the North Sulawesi Region, Indonesia, particularly in termite distribution, control, and economic impact in residential zones. This study aimed to (i) gather preliminary data on termite species in Minahasa and North Sulawesi from the literature; (ii) locate termite colonies via house/building surveys and baiting; (iii) map the distribution of identified termite species by Geographical Information System (GIS); and (iv) record environmental characteristics relevant to termite distribution and related damages. The study revealed a diversity of residential termite species in terms of morphological characteristics and habitats. Three termite families were identified: Rhinotermitidae, Termitidae, and Kalotermitidae, encompassing five sub-families and six genera, with the most notable species being Cryptotermes brevis Walker 1853, Odontotermes javanicus Holmgren, 1912, and Nasutitermes atripennis Haviland, 1898 among others. The Cryptotermes and Odontotermes were the most commonly encountered genera in Minahasa Raya's residential areas.
PENGENDALIAN HAMA KUMBANG LOGONG (Sitophylus oryzae L.) DENGAN MENGGUNAKAN EKSTRAK BIJI PANGI (Pangium edule Reinw.) Ernest H. Sakul; Jacklin S.S. Manoppo; Dalvian Taroreh; Revfly I.F. Gerungan; Sanusi Gugule
EUGENIA Vol. 18 No. 3 (2012)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.18.3.2012.4094

Abstract

ABSTRACT   The research was aimed to prove the effectiveness of seed extract pangi (Pangium edule Reinw.) In increasing mortality of rice-weevil or logong (Sitophylus oryzae L.) using the solvent n-hexane fraction and the fraction of ethanol, and then evaporated with a rotary evaporator to obtain viscous extract. This research was conducted in the Chemistry Laboratory of MIPA Manado State University and mini green house Gapoktan Pinaesaan Tonsealama Village during the months of January, February and March 2012 using an experimental method in a plastic box using Completely Randomized Design (CRD) with six treatments were repeated four times, so the total experimental units were 24 units. Each plastic box filled with 20 of rice-weevil or logong adult and any treatment administered doses of: 2.5 ppm, 5.0 ppm, 7.5 ppm, 15 ppm, 30 ppm and 45 ppm. The results indicate that pangi seed extract is very effective in improving mortality of rice-weevil or logong, where the concentration of the extract  45 ppm is the best pangi seed extract. Test results of Phytochemical Test showed that secondary metabolite of pangi seed extract with n-hexane solvent contains alkaloid compounds and pangi seed extract with ethanol solvent containing phenolic compounds, saponins and tannins. Keywords : extracts, maseration, Pangium edule Reinw., Sitophylus oryzae L., n-heksan fraction, ethanol fraction, bioinsecticide ABSTRAK   Penelitian ini bertujuan untuk membuktikan efektifitas ekstrak biji pangi (Pangium edule Reinw.) dalam meningkatkan mortalitas kumbang logong dengan menggunakan pelarut fraksi n-heksan maupun fraksi etanol, kemudian dievaporasi dengan rotary evaporator guna mendapatkan ekstrak kental.  Penelitian ini dilaksanakan di laboratorium kimia FMIPA Universitas Negeri Manado dan  mini green house Gapoktan Pinaesaan Desa Tonsealama selama bulan Januari, Februari dan Maret 2012 dengan menggunakan metode eksperimen pada kotak plastik menggunakan rancangan acak lengkap (RAL) dengan 6 perlakukan yang diulang sebanyak 4 kali sehingga total unit percobaan sebanyak 24 unit percobaan. Masing-masing kotak plastik diisi dengan 20  ekor kumbang logong dewasa dan setiap perlakuan  diberikan dosis 2,5 ppm, 5,0 ppm, 7,5 ppm, 15 ppm, 30 ppm, dan 45 ppm.  Hasil penelitian menunjukkan bahwa ekstrak biji pangi sangat efektif dalam meningkatkan mortalitas hama kumbang logong dimana pada konsentrasi  ekstrak sebesar 45 ppm merupakan konsentrasi ekstrak biji pangi yang terbaik dalam penelitian ini.  Hasil pemeriksaan uji fitokimia menunjukkan bahwa metabolit sekunder dari ekstrak biji pangi  dengan pelarut n-heksan mengandung senyawa alkaloid dan ekstrak biji pangi dengan pelarut etanol mengandung senyawa fenol, saponin dan tanin. Eugenia Volume 18 No. 3  Desember 2012 Kata kunci : ekstrak, maserasi, Pangium edule Reinw., Sitophylus oryzae L., fraksi n-heksan, fraksi etanol,  insektisida nabati