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Analisis Keragaman Genetik Tikus Hutan Ekor Putih (Maxomys hellwandii) Berbasis RAPD Mege, Revelson A.; Rompas, Christny Ferdina; Wulandari, Wulandari
JSME (Jurnal Sains, Matematika & Edukasi) Vol 8, No 2 (2020): MARET - APRIL
Publisher : Universitas Negeri Manado

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White-tailed forest mice (Maxomys hellwandii) are endemic to the Sulawesi. This study aims to determine the genetic diversity of white-tailed forest mice (Maxomys hellwandii) based on RAPD. The analysis of the variability of white-tailed forest rats was carried out in several stages, namely the extraction and purification of DNA, the amplification of the target gene using the PCR method, and finally visualization of the amplification results with 0.8% agarose gel electrophoresis. Data analysis using the SPSS IBM Ver. 20 program. Results obtained using OPA-3 primers from four different localization samples show samples of Likupang 1 and South Minahasa show similarity of 1%, and samples of Likupang 2 and Southeast Minahasa show similarity of 25%. Whereas the primary OPA-2 samples from the villages of Likupang 1, Likupang 2 and South Minahasa show a similarity of 8% and the sample of Southina Minahasa has a similarity of 25%. On the basis of the dendogram grouping of four different samples, he showed genetic diversity.

Isolasi dan Identifikasi Bakteri Asam Laktat dari Fermentasi Anggur Buah Pala (Myristica fragrans Houtt) Sebagai Penghasil Eksopolisakarida Lawalata, Helen Joane; Rompas, Christny Ferdina; Kansile, Elma Fridayanti
JSME (Jurnal Sains, Matematika & Edukasi) Vol 8, No 1 (2020): JANUARI-FEBRUARI
Publisher : Universitas Negeri Manado

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. Nutmeg fruit is widely used in the medicine formula and also various kinds of processed foods and beverages. Nutmeg meat can be processed into syrup, fruit juice, jam, dodol pala and wine. The fruit is believed to be one of the natural habitats of bacteria, including lactic acid bacteria (BAL). The study aims to determine the type of BALES isolated in nutmeg grapes (Myristica fragrans Houtt) which has the ability to produce exopolysaccharides. Based on the results of the study obtained 15 BAL isolates namely AP1, AP2, AP3, AP4, AP5, AP6, AP7, AP8, AP9, AP10, AP11, AP12, AP13, AP14, and AP15. The exopolysaccharides produced by the isolates are 400 mg/L, 530 mg/L, 1200 mg/L, 730 mg/L, 60 mg/L, 460 mg/L, 600 mg/L, 200 mg/L, 930 mg/L, 1130 mg/L, 200 mg/L, 130 mg/L, 1000 mg/L, 800 mg/L. The next 15 isolates are identified using the profile matching method. The results of the identification show that 15 BAL isolates were included in the genus Lactobacillus and are a species of Lactobacillus bulgaricus.Â Â Â Â Keyword : Isolation, Identification, nugmeg grapes (myristica fragrans houtt), lactic acid bacteria, exopolysaccharides

POTENSI ANTI-INFLAMASI EKSTRAK ETANOL KULIT BUAH MANGGIS (Garcinia mangostana L.) TERHADAP EDEMA TELAPAK KAKI TIKUS PUTIH (Rattus norvegicus) YANG DIINJEKSI FORMALIN Umboh, Aprillia V.; Mege, Revolson A.; Rompas, Christny F. E.
JSME (Jurnal Sains, Matematika & Edukasi) Vol 5, No 2 (2017): Jurusan Biologi
Publisher : Universitas Negeri Manado

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Inflamasi merupakan respon pertahanan tubuh terhadap invasi benda asing. Efek samping dari penggunaan obat-obatan anti-inflamasi steroid seperti asam lambung mendorong perkembangan obat-obatan dari tumbuh-tumbuhan. Kulit buah manggis (Garcinia mangostana L.) merupakan salah satu tumbuhan yang memiliki potensi sebagai obat anti-inflamasi karena memiliki senyawa xanthon dan flavonoid. Tujuan penelitian ini adalah untuk mengetahui potensi antiinflamasi ekstrak etanol kulit buah manggis terhadap edema telapak kaki tikus putih (Rattus norvegicus) yang diinjeksi formalin dengan pengukuran jangka sorong digital. Pengukuran potensi anti-inflamasi digunakan 3 kelompok perlakuan yang berbeda yaitu cataflam (kalium diklofenak) sebagai kontrol positif; aquades sebagai kontrol negatif dan ekstrak etanol kulit buah manggis dosis 150 mg/kgBB. Hasil penelitian menunjukkan bahwa, penurunan edema telapak kaki tikus putih kelompok perlakuan ekstrak etanol kulit buah manggis hampir sama sesuai dengan hasil penurunan besar edema telapak kaki tikus putih kelompok positif cataflam yang diukur dalam kisaran mm. Kelompok perlakuan ekstrak etanol kulit buah manggis 150mg/kgBB pada jam ke-1 3,8mm dan pada jam ke-3 3,1mm hampir sama dengan kontrol positif cataflam yaitu pada jam ke-1 3,5mm dan pada jam ke-3 3,1mm. Dari hasil diatas didapat kesimpulan bahwa ekstrak etanol kulit buah manggis (Garcinia mangostana L.) memiliki potensi anti-inflamasi terhadap edema kaki tikus putih (Rattus norvegicus) yang diinjeksi formalin. Kata kunci: Anti-inflamasi, Garcinia mangostana L., Formalin.

Analisis Kandungan Nutrisi pada Daging Tikus Hutan Ekor Putih (Maxomys hellwandii) Kamagi, Decky W.; Rompas, Christny Ferdina; Bawole, Alpon Trisno
JSME (Jurnal Sains, Matematika & Edukasi) Vol 8, No 2 (2020): MARET - APRIL
Publisher : Universitas Negeri Manado

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White-tailed forest rats (Maxomys hellwandii) are other meat-producing animals that are consumed by the Minahasa community as exotic food. This study aims to determine the nutritional content of white-tailed forest rat meat. Nutrient content analysis was performed at the UNIMA chemistry laboratory and the BARISTAND laboratory in Manado. The procedure for this study is the analysis of nutrient content using the procedure of the Association of Official Analytical Chemists (AOAC 2005). The results of research on fresh forest rat meat showed the content of water, ash, fat, crude fiber, protein and carbohydrates 71.17%, 0.97%, 3.17%, 0.03%, 19, 16%, 5.53% and roasted forest rat meat, i.e. 69.14% water, 1.29% fat and 4.5% proteinÂ Â Â

Effect of Dalugha (Cyrtosperma merkusii) Debris Supplementation on Hemoglobin, Cholesterol and Blood Glucose Levels in Broiler Chickens Debby J.J. Rayer; Emma Mauren Moko; Dino Rahardiyan; Anita C.C Tengker; Christny F. Rompas; Livana Dethris Rawung
Jurnal Ilmiah Sains Volume 23 Nomor 1, April 2023
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jis.v23i1.47598

Feed is an important factor that influences the chicken's development and growth. Meanwhile, dalugha debris is a by-product of dalugha processing which is expected to be a source of feed for the growth and development of broiler chickens. The good feed can optimize metabolic processes in the animal so that it can support the process of growth and development of the animal. This study aims to determine the levels of hemoglobin, cholesterol, and blood glucose in broiler chicken that is given dalugha debris supplementation. This study used a completely randomized design (CRD), where as many as 60 docs of unsex chicken used were divided into four treatments and three replications. Each replication consisted of five chickens. The treatment given was the concentration of dalugha debris at several doses: 0%, 0.065%, 0.13%, and 0.26%. The parameters observed were hemoglobin, cholesterol, and blood glucose levels. The results showed that the provision of dalugha debris in the feed significantly (p<0.05) affected the blood glucose level, but did not affect the cholesterol and hemoglobin levels (p>0.05) of the broiler chicken. Keywords: Broiler chicken; blood biochemistry; Cyrtosperma merkusii

IDENTIFICATION OF ANTS (Formicidae) IN THE MANGROVE FOREST AREA OF TATENGESAN VILLAGE, SOUTHEAST MINAHASA Delvy Regina Leunufna; Debby Jacqueline Jochebed Rayer; Christny Ferdina Evie Rompas; Helen Joan Lawalata; Livana Dethris Rawung
Indonesian Biodiversity Journal Vol. 4 No. 2 (2023): August 2023
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v4i2.6275

Ants are a group of social insects that belong to the order Hymenoptera and the Family Formicidae. This study aims to determine the type of ants (Formicidae) in the Mangrove Area of Tatengesan Village, Southeast Minahasa. This research occurred in Tatengesan Village, Posumaen District, Southeast Minahasa Regency, North Sulawesi. The research method uses a purposive sampling method with the placement of the paths determined based on considerations taking into account the physical and biological conditions that affect the presence of ants in the field. Observation lines were made in as many as three transects, each with four observation plots. Data was collected using adhesive paper traps (Fly Sheet) and direct capture method (hand collecting) and analyzed using the Shannon-Weiner diversity index, species richness index, and species evenness index. Placement of self-adhesive paper on each transect, including lower tree stratum, highest tide limit, middle tree stratum, and upper tree stratum. Based on the results of the research on the observation transect, there were five genera, namely Anoplolepis with 25 individual ants, Camponotus with 31 individual ants, Crematogaster with 98 individual ants, Oecophylla 2168 ants, and Tetraponera 6 individual ants from 6 species of ants consisting of Anoplolepis gracilipes, Camponotus atriceps, Camponotus sp., Crematogaster scutellaris, Oecophylla smaragdina, and Tetraponera nigra. The highest number of genera obtained was Oecophylla, the highest number of species was Oecophylla smaragdina, and the number of individuals caught was 2168.

Pengaruh Suplementasi Kurkumin dan Injeksi Hormon Tiroksin Pada Ikan Mas (Cyprinus carpio L) Terhadap Konsentrasi Hormon Tiroksin dan Laju Pertumbuhan Spesifik Livana Dethris Rawung; Christny Ferdina Evie Rompas
Buletin Anatomi dan Fisiologi Volume 8, Nomor 1, Tahun 2023
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.8.1.2023.29-35

Hormon tiroksin merupakan salah satu jenis hormon tiroid yang berperan penting dalam merangsang pertumbuhan dan perkembangan gonad. Sedangkan kurkumin merupakan senyawa bioaktif yang dapat meningkatkan proses reproduksi pada ikan. Penelitian ini bertujuan untuk mengetahui profil konsentrasi hormon tiroksin dan pertumbuhan spesifik ikan uji yang diberi suplementasi kurkumin dan penyuntikan hormon tiroksin. Ikan mas betina yang digunakan dalam penelitian ini sebanyak 40 ekor. Rancangan percobaan yang digunakan adalah rancangan acak lengkap dengan faktorial 4 x 2. Faktor pertama adalah dosis kurkumin yang terdiri dari 4 konsentrasi (0; 2.5; 5; 10 g curcumin/kg feed), dan faktor kedua adalah hormon tiroksin yang terdiri dari 2 konsentrasi (0 dan 0.1 Âµg.g-1 bobot badan). Secara statistik data hasil penelitian menunjukkan bahwa suplementasi kurkumin dan penyuntikan hormon tiroksin berpengaruh nyata (p<0,05) terhadap konsentrasi hormon tiroksin dan laju pertumbuhan spesifik. Penelitian ini menunjukkan bahwa injeksi hormon tiroksin dan suplementasi kurkumin dapat meningkatkan konsentrasi hormon tiroksin dalam serum, dan hasil ini juga menunjukkan bahwa status reproduksi ikan uji berpengaruh terhadap peningkatan konsentrasi hormon tiroksin dan laju pertumbuhan spesifik.Â Thyroxine hormone is one type of thyroid hormone that plays an important role in stimulating growth and gonad development. Otherwise, curcumin is a bioactive compound that can improve the process of reproduction in fish.Â The aim of this study is to know the profile of the thyroxine hormone concentration and specific growth of tested fish given supplementation of curcumin and injection of thyroxine hormone. 40 female carp were used in this study. The experimental design used was a completely randomized design with a 4 x 2 factorial. The first factor was the dose of curcumin (0; 2.5; 5; 10 g curcumin/kg feed), and the second factor was thyroxine hormone (0 and 0.1 Âµg.g-1 body weight). Statistically, showed that the supplementation of curcumin and injection of thyroxine hormone had a significant effect (p<0.05) on the concentration of the thyroxine hormone and the specific growth rate. This study showed that the injection of the thyroxine hormone and supplementation of curcumin can increase the concentration of the thyroxine concentration in serum, and these results also showed that the reproduction status of the tested fish also influenced the increase of the thyroxine hormone concentration in serum and the specific growth rate (SGR).

PENERAPAN STRATEGI DISCOVERY LEARNING UNTUK MENINGKATKAN HASIL BELAJAR PESERTA DIDIK Stephanie Claudy Manurung; M. H Sumampow; Christny F. E Rompas
SOSCIED Vol 6 No 2 (2023): SOSCIED - November 2023
Publisher : LPPM Politeknik Saint Paul Sorong

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32531/jsoscied.v6i2.742

One of the teacher's tasks is to develop learning methods. An example of a method that can be used is discovery learning. The aim of this research is to discuss improving student learning outcomes on virus material through the application of the Discovery Learning strategy. Due to this, this study will use this learning strategy to improve student learning outcomes on viral material. This study focuses on finding out the application of the Discovery Learning Strategy in improving learning outcomes for class X students at SMAN 1 Maesaan for the 2022/2023 academic year. The researcher used the PTK research strategy with the research subjects being 24 class X MIA 1 students. This study uses observation sheets to collect data. The results of the research show that there is an increase in student learning outcomes for viral material. This is proven by the average score in the first cycle posttest of 70.16. The average value that increased in the second cycle posttest was 77.3%. Therefore, we can conclude that Discovery Learning is able to improve the learning outcomes of class X MIA 1 SMAN 1 Maesaan students.

IDENTIFICATION OF ANTS (Formicidae) IN THE MANGROVE FOREST AREA OF TATENGESAN VILLAGE, SOUTHEAST MINAHASA Delvy Regina Leunufna; Debby Jacqueline Jochebed Rayer; Christny Ferdina Evie Rompas; Helen Joan Lawalata; Livana Dethris Rawung
Indonesian Biodiversity Journal Vol. 4 No. 2 (2023): August 2023
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v4i2.6275

Ants are a group of social insects that belong to the order Hymenoptera and the Family Formicidae. This study aims to determine the type of ants (Formicidae) in the Mangrove Area of Tatengesan Village, Southeast Minahasa. This research occurred in Tatengesan Village, Posumaen District, Southeast Minahasa Regency, North Sulawesi. The research method uses a purposive sampling method with the placement of the paths determined based on considerations taking into account the physical and biological conditions that affect the presence of ants in the field. Observation lines were made in as many as three transects, each with four observation plots. Data was collected using adhesive paper traps (Fly Sheet) and direct capture method (hand collecting) and analyzed using the Shannon-Weiner diversity index, species richness index, and species evenness index. Placement of self-adhesive paper on each transect, including lower tree stratum, highest tide limit, middle tree stratum, and upper tree stratum. Based on the results of the research on the observation transect, there were five genera, namely Anoplolepis with 25 individual ants, Camponotus with 31 individual ants, Crematogaster with 98 individual ants, Oecophylla 2168 ants, and Tetraponera 6 individual ants from 6 species of ants consisting of Anoplolepis gracilipes, Camponotus atriceps, Camponotus sp., Crematogaster scutellaris, Oecophylla smaragdina, and Tetraponera nigra. The highest number of genera obtained was Oecophylla, the highest number of species was Oecophylla smaragdina, and the number of individuals caught was 2168.

DIVERSITY OF THE ORDER HYMENOPTERA IN RICE PLANTATION IN MANEMBO-NEMBO DISTRICT AS AN ECOLOGICAL INDICATOR Gulbudin Hikmatiar Idris; Christny Ferdina Evie Rompas; Utari Satiman; Johanna Zusye Wantania
Indonesian Biodiversity Journal Vol. 4 No. 3 (2023): December 2023
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v4i3.7945

This research aims to determine habitat conditions as an ecological indicator and measure the level of diversity of insects of the order Hymenoptera on rice plantations in the Manembo-nembo sub-district. The method used in this research is quantitative descriptive. Insect samples were collected using the sweeping net and yellow pan trap methods. The Shannon-Wiener diversity index formula, Pielou's similarity index (E), and Simpson dominance index (C) are used to measure the level of diversity. The results of research on rice plantations found insects of the Hymenoptera order as many as 35 genera, 16 subfamilies and 14 families with a total of 470 individuals with normal habitat conditions where Hymenoptera insects can still carry out activities on rice plantations. Wasp insects from 24 genera also have functions as biological control agents, most of which are parasitoid and predatory wasps, which have potential as ecological indicators in rice plantations in the Manembo-nembo sub-district. The level of diversity is included in the medium criteria, as is the evenness index value. In contrast, the dominance index is included in the low criteria even though the Pimplinae family dominates rice cultivation in the Manembo-nembo sub-district.

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