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All Journal Jurnal Sain Veteriner JURNAL ILMIAH PETERNAKAN TERPADU
Prihanani, Nur Ika
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Earth & Planetary Sciences Veterinary Other

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Deteksi Bakteri Penyebab Mastitis Subklinis pada Kambing Peranakan Etawah di Kokap, Kulon Progo, Daerah Istimewa Yogyakarta Artdita, Clara Ajeng; Andityas, Morsid; Prihanani, Nur Ika; Budiyanto, Yohanes Wawan
Jurnal Sain Veteriner Vol 38, No 1 (2020): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (717.872 KB) | DOI: 10.22146/jsv.57365

Abstract

Resident in Kokap, Kulon Progo, Yogyakarta Province has another income sector, that is dairy goat (Etawah Crossbreed) farming and they have been joining into farm group, which is called Kelompok Ternak. There are many bacteria that causing dairy goat intra mamary infection, thus the milk production will be contaminated. Two hundred and four (204) milk samples from 103 lactation goat in four farm groups in Kokap, Kulon Progo, Yogyakarta is used in this study. Out of these, 21 samples (10,29%) were detected strong positive (++ and +++) for subclinical mastitis by using California Mastitis Test (CMT). Among this positive result, the next step was bacteria detection. This tests were held in Preclinic Laboratory Animal Health Study Program, Vocational School, UGM. The samples were inoculated in blood agar, then colony was identify by Gram staining. The next steph is biochemical tests using McConkey Agar (MCA) and eosin methylene blue (EMB) as a selective media for Gram negative bacteria, manitol salt agar (MSA) as selective media for Staphylococcus, sugar fermented broth (glucose, lactose, mannitol, maltose, and sacharose), Kigler iron agar (KIA), lysine iron agar (LIA), motility indole ornithin (MIO), and Simmon’s citrate. The result was Staphylococcus aureus (80%), Staphylococcus epidermidis (20%), Escherichia coli (10%), and Klebsiella pneumoniae (10%). 
Biofilm Properties and Their Association to Antibiotic Resistance of Staphylococcus aureus from Animal Isolates Aziz, Fatkhanuddin; Fitriana, Fauziah; Setyorini, Dian Ritma; Prihanani, Nur Ika; Andityas, Morsid
JURNAL ILMIAH PETERNAKAN TERPADU Vol. 13 No. 2 (2025)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jipt.v13i2.p558-571

Abstract

Staphylococcus aureus (S. aureus) is a Gram positive bacterium that has many virulence factor including the ability to produce biofilm. Biofilm formation is one of the important ability in the persistance and resistant to antibiotic treatment. This study aimed to determine the phenotypic and genotypic characteristics of S. aureus isolates from animal origin in their capacity to produce biofilm in vitro. Eight S. aureus isolates collection from goat mastitis and raw chicken meat origin were phenotypically evaluated the ability to produce biofilm in 96 well culture plate, while genotypic were determinated by detecting the icaA, icaC and icaD genes. We employed  minimum of inhibitory concentration (MIC) data from previous research to clarify their correlation to biofilm production in respected isolates. The results showed that 75% (6/8) of S. aureus isolates had ability to produced biofilm, whereas 50% (4/8) showed the elevation of biofilm production after glucose was added. PCR determination showed that majority isolates were positive for icaA, icaC and icaD genes, while one of the isolates was negative for the icaA. The statistical analysis tests indicated no correlation between the optical density of biofilm production and MIC of antibiotics. Further research is needed to clarify the association of biofilm and antibiotic resistance.
Komparasi Metode coa Polymorphism dan coa Typing pada Bakteri Staphylococcus aureus yang Diisolasi dari Sumber Berbeda Aziz, Fatkhanuddin; Fitriana, Fauziah; Setyorini, Dian Ritma; Prihanani, Nur Ika; Putri, Shafira Amalia; Maulina, Tifa Restyka; Dewi, Vira Kartika; Andityas, Morsid; Lestari, Fajar Budi; Hidayah, Nurulia; Ummami, Risa; Fauzi, Achmad
Jurnal Sain Veteriner Vol 42, No 1 (2024): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.89815

Abstract

Staphylococcus aureus (S. aureus) merupakan golongan bakteri koagulase positif yang menyebabkan berbagai macam penyakit klinis pada manusia dan hewan. Determinasi pola gen coa merupakan salah satu metode yang sering digunakan dalam lingkup studi epidemiologi S. aureus. Penelitian ini dilakukan untuk membandingkan metode coa polymorphism dan coa typing pada isolat S. aureus yang diisolasi dari sumber berbeda. Tujuh belas koleksi isolat S. aureus asal susu pasteurisasi, susu mastitis kambing peranakan etawa, dan daging ayam segar ditumbuhkan dari stok gliserol -80oC, kemudian dilakukan ekstraksi DNA dan amplifikasi gen penyandi koagulase (coa) dengan teknik PCR menggunakan primer untuk coa polymorphism dan coa typing. Diketahui, level diskriminasi coa typing lebih variatif dibandingkan dengan coa polymorphism pada 17 isolat yang diuji. Metode coa typing menunjukkan 7 tipe berbeda, sedangkan coa polymorphism hanya 3. Empat dari 17 isolat tidak dapat ditentukan  coa typing, menunjukkan potensi pengembangan tipe baru coa typing untuk isolat-isolat asal Indonesia, untuk kepentingan studi epidemiologi.
Co-Authors Achmad Fauzi Budiyanto, Yohanes Wawan Clara Ajeng Artdita, Clara Ajeng Dewi, Vira Kartika Fajar Budi Lestari Fatkhanuddin Aziz, Fatkhanuddin Fitriana, Fauziah Hidayah, Nurulia Maulina, Tifa Restyka Morsid Andityas Putri, Shafira Amalia Risa Ummami, Risa Setyorini, Dian Ritma