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All Journal Jurnal Sain Veteriner Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Sarwahita : Jurnal Pengabdian Kepada Masyarakat Jurnal Pengabdian dan Pengembangan Masyarakat Journal of Livestock and Animal Health (JLAH) Buletin Veteriner Udayana
Clara Ajeng Artdita
Department Of Bioresources Technology And Veterinary, Vocational College, University Of Gadjah Mada, Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Public Health Social Sciences Veterinary Other

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Deteksi Bakteri Penyebab Mastitis Subklinis pada Kambing Peranakan Etawah di Kokap, Kulon Progo, Daerah Istimewa Yogyakarta Artdita, Clara Ajeng; Andityas, Morsid; Prihanani, Nur Ika; Budiyanto, Yohanes Wawan
Jurnal Sain Veteriner Vol 38, No 1 (2020): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (717.872 KB) | DOI: 10.22146/jsv.57365

Abstract

Resident in Kokap, Kulon Progo, Yogyakarta Province has another income sector, that is dairy goat (Etawah Crossbreed) farming and they have been joining into farm group, which is called Kelompok Ternak. There are many bacteria that causing dairy goat intra mamary infection, thus the milk production will be contaminated. Two hundred and four (204) milk samples from 103 lactation goat in four farm groups in Kokap, Kulon Progo, Yogyakarta is used in this study. Out of these, 21 samples (10,29%) were detected strong positive (++ and +++) for subclinical mastitis by using California Mastitis Test (CMT). Among this positive result, the next step was bacteria detection. This tests were held in Preclinic Laboratory Animal Health Study Program, Vocational School, UGM. The samples were inoculated in blood agar, then colony was identify by Gram staining. The next steph is biochemical tests using McConkey Agar (MCA) and eosin methylene blue (EMB) as a selective media for Gram negative bacteria, manitol salt agar (MSA) as selective media for Staphylococcus, sugar fermented broth (glucose, lactose, mannitol, maltose, and sacharose), Kigler iron agar (KIA), lysine iron agar (LIA), motility indole ornithin (MIO), and Simmon’s citrate. The result was Staphylococcus aureus (80%), Staphylococcus epidermidis (20%), Escherichia coli (10%), and Klebsiella pneumoniae (10%). 
Klebsiella pneumoniae Isolated from Subclinical Mastitis Milk of Etawah Crossbreed Goat Clara Ajeng Artdita; Fajar Budi Lestari; Achmad Fauzi; Erian Pemila Ayu Tanzila
Jurnal Sain Veteriner Vol 36, No 2 (2018): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (9907.598 KB) | DOI: 10.22146/jsv.34319

Abstract

Klebsiella spp. is a common bacteria causing mastitis. Klebsiella pneumoniae and Klebsiella varicola is the most infected Klebsiella spp in the dairy farm. This study used 16 milk samples from 8 lactation goats in Sahabat Ternak farm, Sleman, Yogyakarta. Samples were tested by california mastitis test (CMT) reagent to determine the mastitis status, inoculated on blood agar (BA) then on Mac Conkey Agar (MCA) as a selective media for Gram negative bacteria. The colonies from BA and MCA were stained by Gram staining to determine Gram-positive or Gram-negative bacteria and its cell morphology. The colonies from MCA were identified by biochemical tests such as sugar fermentation tests (glucose, lactose, maltose, and saccharose), arginine, ornithine decarboxylase, indole, citrate, urea hydrolysis (Christensen’s method), lysin and malonate. The result of mastitis test showed 5 goats were positive result and 3 samples were negative. Bacterial staining showed 2 samples were Gram-negative, rods and the others were Gram-positive, coccus. The samples with rods shapes were continued by biochemical tests. The characterization result of biochemical test indicated that the rods shapes bacteria were Klebsiella pneumoniae. These bacteria identification conducted in Laboratorium Preklinis Program Studi Kesehatan Hewan, Sekolah Vokasi Universitas Gadjah Mada and the result confirmation using positive control of  Klebsiella pneumoniae conducted in Balai Laboratorium Kesehatan Yogyakarta. These results showed that milk from Etawah crossbreed goat was infected by Klebsiella pneumoniae.
Bacterial Detection Causing Subclinical Mastitis on Etawah Crossbreed Goat in Kokap, Kulon Progo, Yogyakarta Province Clara Ajeng Artdita; Morsid Andityas; Nur Ika Prihanani; Yohanes Wawan Budiyanto
Jurnal Sain Veteriner Vol 38, No 1 (2020): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.39818

Abstract

Resident in Kokap, Kulon Progo, Yogyakarta Province has another income sector, that is dairy goat (Etawah Crossbreed) farming and they have been joining into farm group, which is called Kelompok Ternak. There are many bacteria that causing dairy goat intra mamary infection, thus the milk production will be contaminated. Two hundred and four (204) milk samples from 103 lactation goat in four farm groups in Kokap, Kulon Progo, Yogyakarta is used in this study. Out of these, 21 samples (10,29%) were detected strong positive (++ and +++) for subclinical mastitis by using California Mastitis Test (CMT). Among this positive result, the next step was bacteria detection. This tests were held in Preclinic Laboratory Animal Health Study Program, Vocational School, UGM. The samples were inoculated in blood agar, then colony was identify by Gram staining. The next steph is biochemical tests using McConkey Agar (MCA) and eosin methylene blue (EMB) as a selective media for Gram negative bacteria,  manitol salt agar (MSA) as selective media for Staphylococcus, sugar fermented broth (glucose, lactose, mannitol, maltose, and sacharose), Kigler iron agar (KIA), lysine iron agar (LIA), motility indole ornithin (MIO), and Simmon’s citrate. The result was Staphylococcus aureus (80%), Staphylococcus epidermidis (20%), Escherichia coli (10%), and Klebsiella pneumoniae (10%). 
Identifikasi Molekuler Bakteri Staphylococcus sp. dan Staphylococcus aureus Penyebab Mastitis Subklinis pada Ternak Kambing Perah Clara Ajeng Artdita; Fatkhanuddin Aziz; Nurulia Hidayah; Achmad Fauzi; Triastuti Septi Wulandari; Reza Luthfi Hamid
Jurnal Sain Veteriner Vol 39, No 2 (2021): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.60557

Abstract

Mastitis merupakan radang pada glandula mammae (ambing) ternak perah. Mastitis tipe subklinis sering dikaitkan pada kejadian mastitis di peternakan ruminansia kecil seperti kambing perah (kambing Peranakan Etawah, Saanen, dan Sapera). Patogen utama yang berperan dalam kejadian mastitis ini adalah genus Staphylococcus. Tujuan penelitian adalah untuk melakukan identifikasi bakteri Staphylococcus sp. dan Staphylococcus aureus sebagai penyebab mastitis subklinis pada kambing perah dengan menggunakan metode polymerase  chain reaction (PCR). Tahapan metode yang dilakukan adalah ekstraksi DNA dengan teknik spin-collumn system terhadap 26 isolat bakteri yang telah dilakukan uji biokimia sebelumnya dan amplifikasi gen spesifik 23s rRNA Staphylococcus sp. dan Staphylococcus aureus, serta methicillin resistant Staphylococcus aureus (MRSA), dilanjutkan dengan visualisasi menggunakan UV-transluminator. Hasil menunjukkan bahwa sebanyak 12 isolat sampel teridentifikasi Staphylococcus sp. dan 1 diantaranya teridentifikasi Staphylococcus aureus. Isolat yang teridentifikasi Staphylococcus aureus bukan termasuk MRSA. 
PENDAMPINGAN KEGIATAN DIPPING PUTING PADA TERNAK KAMBING PERAH DI DESA TURGO, TURI, SLEMAN, YOGYAKARTA Clara Ajeng Artdita; Morsid Andityas; Ronny Martien; Maria Advensia Eka Setyaningrum; Achmad Fauzi
Bahasa Indonesia Vol 17 No 01 (2020): Sarwahita : Jurnal Pengabdian Kepada Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/sarwahita.171.01

Abstract

Abstract Dipping is one of the main process for the stage of milking process and it serves to prevent the the bacteria infected the udder on post-milking process. Mastitis can occur because of this stage not being carried out. The program partner is in the Turgo village, Turi, Sleman, Yogyakarta. This partner has obstacles in the magement of Ettawa crossbreed (PE) goat productivity including milking goat Standard Operating Management (SOP). The purpose of t his community service is to make farmer feel easier to do the dipping method by using herbal-based products sprayer to be applied to PE goats after milking to prevent occurence of mastitis. The method of this program is introducing PE goat cultivation by applying the concept of good farming practices and the importance of preventing mastitis, utilizing home yard to plant Piper betle linn. As a basis for making teat dip sprayer, and improving the productivity of PDE goat as dairy goat with herbal teat dip sprayer using ionic gelation method. Teat dip sprayer technology on dairy goats is expected to increase the productivity of PE goat milk in this partner “Kelompok Ternak”. The result is an increasing of farmer’s understanding and the sustainability of milking SOP of dipping with herbal products which easy to apply (sprayer); So that the livestock health and productivity are increases, then it will impact in economic of this farmer community.Abstrak Dipping merupakan proses pencelupan puting ambing ternak perah yang termasuk ke dalam tahapan proses pemerahan susu dan berfungsi untuk mencegah berkembangnya bakteri paska pemerahan. Kasus mastitis dapat terjadi akibat tahapan ini tidak dilakukan usai pemerahan. Mitra program ini adalah kelompok ternak di desa Turgo, Turi, Sleman, Yogyakarta. Mitra ini memiliki kendala dalam manajemen pengelolaan kambing PE produktif termasuk Standar Operasional Prosedur (SOP) dalam pemerahan susu kambing. Tujuan dari pengabdian ini adalah untuk memudahkan petani peternak dalam melakukan metode dipping dengan produk berbahan herbal untuk diaplikasikan pada ternak perahnya usai dilakukan pemerahan sebagai agen antibakterial untuk mencegah kejadian mastitis. Metode yang akan dilakukan melalui program ini yaitu memperkenalkan budidaya kambing PE dengan menerapkan konsep good farming practices dan pentingnya pencegahan mastitis, memanfaatkan pekarangan rumah untuk ditanami daun sirih hijau sebagai dasar pembuatan dipping sprayer, dan memperbaiki produktivitas ternak perah dengan herbal dipping sprayer menggunakan metode ionic gelation. Pemanfaatan teknologi dipping sprayer pada kambing perah diharapkan dapat meningkatkan produktivitas susu kambing PE di Kelompok Ternak mitra ini. Hasil akhir berupa terbentuknya pemahaman peternak serta keberlanjutan SOP pemerahan berupa dipping dengan produk yang mudah pengaplikasiannya (spray dipping) sehingga ternak sehat, produktivitas meningkat, dan ekonomi masyarakat pedesaan mampu terangkat.
Pembentukan dan Pembinaan Kelompok Tani Kelinci dalam Rangka Pengembangan Desa Wisata di Soropati, Hargotirto, Kokap, Kulon Progo Naela Wanda Yusria Dalimunthe; Clara Ajeng Artdita; Fajar Budi Lestari
Jurnal Pengabdian dan Pengembangan Masyarakat Vol 2, No 1 (2019): MAY
Publisher : Pengabdian dan Pengembangan Masyarakat Sekolah Vokasi UGM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jp2m.48334

Abstract

Community Service Activities in 2018 have been implemented in Soropati Village, Hargotirto, Kokap, Kulon Progo. The purpose of this activity was to provide information on the maintenance and formation of rabbit farmer groups by involving the academics of the Diploma in Animal Health Program, Department of Biological and Veterinary Technology, Vocational School, Gadjah Mada University with Animal Health Centre in Kokap so that a better  communication between academics, officers, and breeders community should be conducted well. The activity is carried out by providing information and training transfers regarding the management of rabbit maintenance including rabbit species, housing management, feed, and breeding. The implementation of this program was carried out by giving professional speech and interactive discussions then comparative studies to rabbit farmers who had been successful, counseling and fostering. The result of this activity is that the Mantep Makaryo farmer group has been formed which is engaged in the maintenance and cultivation of rabbits. Development of sustainable breeders needs to be carried out to improve health management and the development of rabbit farms.
Komparasi Lima Jenis Primer Polymerase Chain Reaction Untuk Mengidentifikasi Kelamin Burung Famili Columbidae Yang Akurat Fauziah Fitriana; Riza Resita; Yuda Disastra; Gioknio Happy Alfatik; Clara Ajeng Artdita; Aris Haryanto; Fatkhanuddin Aziz
Jurnal Sain Veteriner Vol 40, No 2 (2022): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.68787

Abstract

Penentuan jenis kelamin pada beberapa spesies burung cukup sulit dilakukan dikarenakan jantan dan betina memiliki ciri morfologi yang sama (monomorfik), salah satunya famili Columbidae. Teknik penentuan jenis kelamin burung secara molekuler yang populer adalah metode Polymerase Chain Reaction (PCR) dengan gen target Chromo Helicase DNA-binding (CHD), namun keberhasilan amplifikasi gen target pada PCR tersebut dipengaruhi salah satunya kesesuaian DNA template dengan primer yang digunakan. Penelitian ini bertujuan untuk mengevaluasi 5 jenis primer PCR P2/P8, 2550F/2718R, CHD1F/CHD1R, 1237L/1272H dan CHD1LF/CHD1LR untuk menentukan jenis kelamin famili Columbidae. Penelitian ini dilakukan dengan menguji 5 jenis primer tersebut di atas pada sampel DNA tiap pasang jantan dan betina dari burung merpati, balam jambi, punai, derkuku, dan perkutut. Hasil penelitian diketahui primer CHD1LF/CHD1LR menunjukkan hasil terbaik dan direkomendasikan untuk menentukan jenis kelamin famili Columbidae.
Faktor Risiko Kejadian Mastitis pada Kambing Peranakan Etawah (PE) di Kelompok Ternak Tirto, Kokap, Kulonprogo, Yogyakarta: The Risk Factor for Mastitis in Peranakan Etawah (PE) Goat at Tirto Farmer Group, Kokap, Kulonprogo, Yogyakarta Artdita, Clara Ajeng; Hidayah, Nurulia; Lestari, Fajar Budi; Budiyanto, Yohanes Wawan; Hidayatullah, Muhammad Fatan; Rahmayanti, Desyah
Journal of Livestock and Animal Health Vol. 4 No. 2 (2021): August
Publisher : Politeknik Pertanian Negeri Payakumbuh

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (200.352 KB) | DOI: 10.32530/jlah.v4i2.300

Abstract

Kelompok Ternak Tirto merupakan salah satu peternakan kambing Peranakan Etawah (PE) yang cukup besar dan berada di wilayah Kulonprogo, Yogyakarta. Kambing Peranakan Etawah dikembangbiakkan untuk produksi susu. Intra Mammary Infection (IMI) atau mastitis, khususnya mastitis subklinis, merupakan salah satu penyakit yang menurunkan produksi susu kambing. Tujuan penelitian ini adalah untuk mengetahui faktor risiko yang berkontribusi terhadap terjadinya mastitis subklinis pada kambing PE di Kelompok Ternak Tirto tersebut. Data faktor risiko dikumpulkan menggunakan kuesioner. Metode kuesioner berupa wawancara langsung kepada 19 peternak anggota kelompok ternak ini dan observasi pada saat dilakukan kegiatan pemerahan. Faktor risiko pada kambing PE ditentukan dengan menggunakan odds ratio (OR). Faktor risiko yang berhubungan positif dengan mastitis subklinis pada kambing PE kelompok tani Tirto di Kokap, Kulonprogo, Yogyakarta adalah kebersihan kandang (OR = 1,2) dan pembersihan ambing (OR = 8,6), sedangkan faktor risiko lainnya adalah asosiasi negatif.
Variasi Karakter Resistensi Antibiotik Bakteri Penyebab Mastitis Subklinis pada Kambing Peranakan Ettawa di Yogyakarta Lestari, Fajar Budi; Artdita, Clara Ajeng; Wasissa, Madarina; Hidayah, Nurulia
Jurnal Sain Veteriner Vol 43, No 1 (2025): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.52529

Abstract

Salah satu penyakit yang sering dijumpai dalam budidaya kambing Peranakan Etawa (PE) adalah mastitis. Beberapa bakteri patogen dapat menyebabkan mastitis diantaranya adalah Staphylococcus sp., E. coli, Klebsiella spp., dan Streptococcus spp. Meningkatnya kejadian penyakit menyebabkan meningkatnya penggunaan antibiotik yang berakibat pada peningkatan potensi resistensi dan residu antibiotik. Penelitian ini bertujuan untuk mengetahui variasi karakter fenotipik resistensi bakteri penyebab mastitis yang diisolasi dari susu kambing PE yang mengalami mastitis subklinis terhadap berbagai golongan antibiotik yang umum dipakai petugas medis veteriner di wilayah Yogyakarta. Sebanyak 8 isolat diperoleh dari peternakan rakyat di wilayah kecamatan Kokap, Kulon Progo,, sedangkan 4 isolat diperoleh dari wilayah Pakem, Sleman, Yogyakarta. Dua belas isolat tersebut terdiri dari 3 jenis bakteri yang berbeda yaitu Staphylococcus sp., Escherichia coli, dan Klebsiella sp. Berdasarkan hasil uji antibiogram diketahui bahwa terdapat perbedaan pola resistensi antibiotik antara isolat bakteri penyebab mastitis dari Kulon Progo dan Sleman. Ampicillin, penicillin G, tetracyclin, dan cefoxitin efektif digunakan untuk pengobatan mastitis di daerah Sleman, sedangkan di Kulon Progo gentamicyn, tetracyclin dan erythromycin adalah pilihan antibiotik yang efektif digunakan
SEMI-EXTENSIVE BEEF CATTLE BREEDING MANAGEMENT IN DOMPU REGENCY, WEST NUSA TENGGARA Febri Ariyanti; M. Yandi Fadilah; Fatkhanuddin Aziz; Muhammad Rosyid Ridlo; Nurulia Hidayah; Nur Ika Prihanani; Ida Tjahajati; Fajar Budi Lestari; Morsid Andityas; Clara Ajeng Artdita
Buletin Veteriner Udayana Bul. Vet. Udayana. December 2025 Vol. 17 No. 6
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i06.p18

Abstract

Beef cattle farming in Dompu Regency, West Nusa Tenggara, is conducted semi-extensively for various purposes, including breeding. This study aimed to identify the beef cattle breeding management practices used by smallholder farmers in Dompu Regency. Data were collected through field observations and interviews using questionnaires administered to 133 farmers in two villages: Dorebara and Adu. The research variables included farmer characteristics, reproductive issues, preferred cattle breeds, breeding management, feeding practices, and livestock health control. The results showed that most farmers were of productive age, had completed secondary education, and operated their farms as a side business driven by economic and socio-religious motivations. The main challenges in semi-extensive beef cattle breeding were limited access to bulls, long calving intervals, and low body condition scores in cows. Farmers preferred Bali cattle and crossbred cattle, and continued to rely on natural mating because it is accessible and cost-effective. The use of legumes such as Leucaena leucocephala during pregnancy and lactation remains suboptimal, as knowledge of feed management is largely based on tradition. Livestock health programs have been supported by the government through annual vaccination campaigns, but farmers’ awareness of livestock health remains low. Beef cattle productivity in semi-extensive systems can be improved through technical assistance, extension services, and better access to superior bulls and high-quality forage.
Co-Authors Achmad Fauzi Achmad Fauzi Achmad Fauzi Aris Haryanto Budiyanto, Yohanes Wawan Erian Pemila Ayu Tanzila Fajar Budi Lestari Fajar Budi Lestari Fajar Budi Lestari Fatkhanuddin Aziz Fatkhanuddin Aziz Fatkhanuddin Aziz, Fatkhanuddin Fauziah Fitriana Febri Ariyanti Gioknio Happy Alfatik Hidayah, Nurulia Hidayatullah, Muhammad Fatan Ida Tjahajati M. Mukhlis Aditya Pangestu M. Yandi Fadilah Maria Advensia Eka Setyaningrum Maria Advensia Eka Setyaningrum Meiandra Ragil Pinesthy Mitra Slipranata Morsid Andityas Morsid Andityas Morsid Andityas Muhammad Rosyid Ridlo Naela Wanda Yusria Dalimunthe Nur Ika Prihanani Nur Ika Prihanani Nur Ika Prihanani Nurulia Hidayah NURULIA HIDAYAH Prihanani, Nur Ika Rahmayanti, Desyah Reza Luthfi Hamid Riza Resita Ronny Martien Sidna Artanto Siti Isrina Oktavia Salasia Suryanto Suryanto Triastuti Septi Wulandari Vidya Maharani Wasissa, Madarina Yohanes Wawan Budiyanto Yuda Disastra