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All Journal Cannarium (Jurnal Ilmu-ilmu Pertanian) Journal of Degraded and Mining Lands Management Jurnal Biologi Tropis Agro Bali: Agricultural Journal Jurnal Pengabdian Masyarakat (ABDIRA) Wana Lstari
Widinugraheni, Sri
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Aerospace Engineering Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Nursing Social Sciences Other

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The potential of phosphate solubilizing microorganisms (PSM) isolated from different ecosystems on calcareous soils in Timor Island, Indonesia Adu Tae, Anthonius S. J.; Nur, Mahmuddin S.M.; Benggu , Yoke Ivonny; Ishaq, Lily F.; Soetedjo, I N. Prijo; Widinugraheni, Sri; Kasim, Muhammad
Journal of Degraded and Mining Lands Management Vol. 12 No. 3 (2025)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2025.123.7803

Abstract

The study was conducted in three different ecosystems: mamar (a typical local ecosystem close to water catchment areas), farm, and coastal ecosystems. In each ecosystem, soil samples were taken from the rhizosphere of five different plants to investigate the occurrence of PSM, molecularly identify the potential isolates, and evaluate their ability to increase soil phosphorus (P) availability and mungbean yield. The highest population of phosphate-solubilizing bacteria (PSB) was found in the mamar ecosystem. Phosphate-solubilizing fungi were only found in the farm and coastal ecosystems, with low populations, and restricted to the rhizosphere of a few plants. The number of PSB isolates was higher in the coastal ecosystem, followed by the farm and mamar ecosystems. The phosphate solubilizing index of the isolate was quite high, ranging from 210 to 300. One isolate from the five molecularly selected isolates from Kupang regency was identified as Aspergillus sp. (cassava rhizosphere origin), one isolate from the farm ecosystem (Moringa rhizosphere origin), and three isolates from the coastal ecosystem (Jatropha gossypiifolioa, Scheichera oleosa and Calotropis gigantea L. rhizosphere origin) were all identified as Pseudomonas sp. Two selected isolates from a previous study in Timor Tengah Selatan regency, collected from the mamar ecosystem (bamboo and Leuchaena leucocephala rhizosphere), were both identified as Acinetobacter baumannii. Inoculation of PSB resulted in higher available soil P compared to the uninoculated treatment. PSB inoculation also provided higher tissue P than the uninoculated treatment. PSB from coastal areas gave the highest pod weight and seed weight compared to other inoculation treatments.  
Peningkatan Teknik Pembuatan Pupuk Organik Majemuk pada Kelompok Kaum Bapak Jemaat GMIT Mizpa Bonen, Kecamatan Taebenu Kabupaten Kupang Pollo, Roddialek; Benggu, Yoke I.; Adu Tae, Anthonius S. J.; Widinugraheni, Sri; Taloim, Aristarkhus; Imanuel Nalle, Ryan Pieter
Jurnal Pengabdian Masyarakat (ABDIRA) Vol 5, No 3 (2025): Abdira, Juli
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/abdira.v5i3.764

Abstract

This service activity has been carried out in the GMIT Mizpa Bonen categorical group, most of whom work as farmers and livestock breeders who are members of the dry land farmer group. The aim of this training is to provide knowledge, understanding and skills about increasing productivity through the use of livestock manure, dry land weeds and plant litter as compound organic fertilizer which has higher economic value and plays a dual role in dry land plant cultivation. The approach method for this activity is lectures and direct practice regarding the application of making compound organic fertilizer. The results achieved from this PKM activity are that the community has increased knowledge and skills in utilizing plant biomass around their living environment as organic fertilizer to support the growth and development of cultivated plants.
Inhibitory response of Pectobacterium carotovorum subsp. carotovorum(Jones) Dye Applied with Betel Leaf and Areca Nut Extract as a Potential Organic Bactericide Uge, Emerensiana -; Harini, Titik Sri; Widinugraheni, Sri; Henuk, Jilinda B.D
Cannarium Vol 18, No 1 (2020)
Publisher : Universitas Khairun

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33387/cannarium.v18i1.1451

Abstract

Soft root caused by the bacterium Pectobacterium carotovorum subsp carotovorum (Jones) Dye (P.c.c), is a disease that is very detrimental to horticultural plants, especially carrot. The disease control is commonly done by chemical techniques, but because of  the negative impact, it is important to choose an environmentally friendly techniques, such as plant extracts. The aim of this research is to know response of P.c.c bacteria growth when it is applied with betel leaves extracts (BTL) and Areca nut extracts (ANE) in different concentrations.This research is begun  with pathogenecity test,  Gram coloring test, and Growing on selective media. While the inhibation testing was carried out in In Vitro. Experiment were carried out with agarose-wells method, where bacterial inoculum was grown with agarose medium, then plant extract added in to medium through the well. The observation done by measured inhibitory zone diameter (mm) around agarose-wells. The data presented the size of the inhibitory zone diameter (mm) formed on P.c.c culture media, then was analyzed  using  ANOVA followed by  Duncan’s  test.Pathogenecity test, Gram-coloring test, and growing selective media test showed that there was symptoms on 4 days after infection, red and rod-shaped bacteria, while in CVD medium, the bacteria grow uniformly at 37 0 C, although they do not form a basin in the medium. And In vitro test showed that the treatment of BTL, and ANE  at 10%, 5%, and 10% concentration respectively, had good ability to inhibit bacteria in NA medium, with the average inhibition zone diameters of 32.33 mm, 28.67 mm, and 33 .67 mm.
Identification and Distribution of Damage Intensity Caused by Sanurus spp. on Cashew Plants in East Flores Regency, Indonesia Kleden, Yasinta L.; Kadja, Don H.; Nahas, Agustina E.; Widinugraheni, Sri; Pramatana, Fadlan
Agro Bali : Agricultural Journal Vol 8, No 2 (2025)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37637/ab.v8i2.2284

Abstract

Cashew is one of Indonesia's leading commodities as an exporting country because it has a positive trade specialization index. The main pest of cashew plants that affects production and can cause crop failure is the Cashew Planthopper (WPM). Cashew Planthopper has several species. Therefore, monitoring is needed to determine the types of WPM in the field and their distribution areas on the mainland of East Flores Regency. This study was conducted from May 2024 to July 2024 by directly capturing WPM imago. The results of a survey of cashew plantation locations in East Flores Regency, 5 locations were determined with high levels of attack due to WPM, which caused crop failure, namely: Ile Mandiri District, Lewolema District, Tanjung Bunga District, Titehena District, and Demon Pagong District. The trapped WPM were then identified by looking at the characteristics of the wings, thorax, and abdomen. The identification results obtained 2 types of WPM from the genus Sanurus, namely, Sanurus flavovenosus and Sanurus indecora. The distribution area of the attack and the level of damage to Sanurus from each district have different intensities with different levels of damage, namely the intensity of heavy damage in red is in Titehena District 53.04%, the intensity of moderate damage in yellow is in Lewolema District 20.65%, Ile Mandiri District 36.91% and Demon Pagong District 36.94% while the intensity of light damage in green is in Tanjung Bunga District 0.55%.
Uji Kemampuan Trichokompos Dalam Menekan Penyakit Layu Fusarium Pada Tanaman Tomat Simamora, Agnes V.; Serangmo, Diana Y. L.; Iburuni, Yohanes U. R.; Widinugraheni, Sri; Hali, Antonius Suban; Abanat, Fani R.
Wana Lestari Vol 4 No 2 (2022): JURNAL KEHUTANAN
Publisher : Program Studi Kehutanan, Fakultas Pertanian, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/wanalestari.v7i02.9466

Abstract

Fusarium wilt is one of the deadly diseases on tomato plants that need to be controlled. Trichocompost is compost containing the antagonistic fungus Trichoderma sp., which increases soil fertility and controls plant pathogens. This study aimed to examine the effect of trichocompost doses on: (1) the growth and yield of tomato plants, (2) the incidence of fusarium wilt, and (3) the severity of fusarium wilt disease in tomato plants.This research was conducted at the Laboratory of Plant Diseases and Greenhouses of Faperta Undana from March 2021 to August 2021. This study used a Randomized Block Design consisting of seven treatments repeated three times, so there were 21 experimental units. The treatments tried were Trichocompost 25 g/polybag two weeks before F. oxysporum inoculation, Trichocompost 50 g/polybag two weeks before F. oxysporum inoculation, Trichocompost 75 g/polybag two weeks before F. oxysporum inoculation, Trichocompost 25 g/polybag two weeks after F. oxysporum inoculation, Trichocompost 50 g/polybag two weeks after inoculation of F. oxysporum, Trichocompost 75 g/polybag two weeks after inoculation of F. oxysporum, andInoculation of F. oxysporum at planting without trichocompost application. The results showed that: (1) Trichocompost 75 g/polybag applied two weeks before Fusarium oxysporum inoculation was able to increase plant height, the number of leaves, and the number of tomato fruits, (2) Trichocompost applied two weeks before and after inoculation of Fusarium oxysporum was able to reduce the incidence of fusarium wilt, (3) Trichocompost 75 g/polybag, applied two weeks before Fusarium oxysporum inoculation, reduced the severity of fusarium wilt and was included in the category of high control effectiveness.
Impact of Blood Disease Bacteria Spread and Economic Losses from Banana Supply Chain Process in Ngada Regency Mau, Maria Clara; Bao, Antonia Paulina; Uta, Maria Edmunda; Limbu, Umbu N.; Widinugraheni, Sri
Jurnal Biologi Tropis Vol. 25 No. 4b (2025): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4b.10628

Abstract

Ngada Regency is one of the regencies in Flores that produces bananas, especially plantains. Kepok banana production has declined over the past four years due to the spread of banana blood disease (BDB). The decrease in harvest yields has resulted in economic losses in the region. This research aims to determine the transportation and distribution mechanisms of banana harvest yields and the handling of bananas in Ngada Regency, the impact of blood disease bacteria on banana transportation and handling, and the economic losses at the farmer producer level, as well as the banana supply chain in Ngada Regency. Additionally, it aims to map the distribution of blood disease bacteria in Ngada Regency. This research employed a qualitative approach using a case study methodology. The data collection techniques in this study consist of three methods: observation, interviews, and documentation. Data from the observation of banana land affected by disease will be displayed in the QGIS mapping program, while data on distribution and transportation mechanisms, handling, economic losses, and the banana supply chain will be analyzed descriptively and qualitatively. The area of banana plantations affected by disease in 2025 is 2,453.21 hectares, covering all areas in Ngada Regency. Traders and distributors supply kepok bananas from the eastern regions of Flores Island, namely Sikka Regency (Maumere) and East Flores Regency (Larantuka), and then sell them to Ngada, Ende, and Manggarai at high prices. Handling of unpackaged bananas is done by covering them directly with tarpaulins during distribution. Banana farmers experienced a drastic economic loss, with a 100% decrease in yield, while traders/collectors saw a 50% decrease in yield and distributors experienced a 63% decrease in yield. The spread of BDB disease has extended throughout the Ngada region, resulting in economic losses for banana farmers, collectors/traders, and distributors. To meet the banana supply chain, bananas are distributed from the Sikka and East Flores Regency.
Co-Authors Abanat, Fani R. Adu Tae, Anthonius S. J. Bao, Antonia Paulina Benggu , Yoke Ivonny Don Harrison Kadja Hali, Antonius Suban Harini, Titik Sri Henuk, Jilinda B.D Iburuni, Yohanes U. R. Imanuel Nalle, Ryan Pieter ISHAQ, LILY Limbu, Umbu N. Mau, Maria Clara Muhammad Kasim Nahas, Agustina E. Nur, Mahmuddin S.M. Pollo, Roddialek Pramatana, Fadlan Serangmo, Diana Y. L. Soetedjo, I N. Prijo Taloim, Aristarkhus Uge, Emerensiana - Uta, Maria Edmunda Yasinta L. Kleden Yoke I. Benggu, Yoke I.