Article Per Year (5 Year)
p-Index From 2020 - 2025
2.096
P-Index
This Author published in this journals
All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Journal of Tropical Biodiversity and Biotechnology BERITA BIOLOGI Jurnal Atma Inovasia Jurnal Kefarmasian Indonesia
Widhiastuti, Stefani Santi
Universitas Atma Jaya Yogyakarta
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Environmental Science Immunology & microbiology Medicine & Pharmacology Social Sciences Other

Published : 12 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 12 Documents
Search

 1   2 
Phytochemical Properties, Antioxidant, and Cytotoxicity Activity of Knobweed (Hyptis capitata) from South Sulawesi, Indonesia To'bungan, Nelsiani; Widhiastuti, Stefani Santi; Hida, Fitriana Nur Laissya; Mahardhika, I Wayan Swarautama
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 3 (2024): September
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.90976

Abstract

Hyptis capitata Jacq. known as Sumambu plants in Sulawesi, has phytopharmaceutical importance. H. capitata extracts were evaluated for their phytochemical properties, antioxidant activity, and cytotoxicity. Using the maceration yielded five types of extracts: root chloroform (RC), root methanol (RM), leaf chloroform (LC), leaf methanol (LM), and leaf ethanol (LE). Phytochemical properties were identified by qualifying procedure and digital image analysis for quantifying Red-Green-Blue (RGB) percentage and hex colour code. 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay was used to determine the half-maximal inhibitory concentration (IC50). Cytotoxicity screening of each extract was performed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay against HeLa and 4T1 cells. Gas Chromatography-Mass Spectrometry (GC-MS) assay was used to identify the phytochemical compounds of the extracts with the most promising potential. Alkaloids were the major constituents of the phytochemicals of RC, RM, LE, LC, and LM. RM and LM have potency and weak free radical scavenging activities, with IC50 value 31.08 and 58.03 µg/mL, respectively. The IC50 of RC and RM against HeLa cells were 84.21 ± 0.63 and 172.10 ± 02.90 µg/mL, respectively. Meanwhile, the cytotoxicity of RC and RM against 4T1 cells were 86.42 ± 0.80 and 182.82 ± 7.00 µg/mL, respectively. It means RC and RM exhibit a moderate level of cytotoxicity in both HeLa and 4T1 cells. LM shows moderate cytotoxicity, but it is limited to 4T1 cells with an IC50 value of 181.86 ± 12.68 µg/mL. The cytotoxicity level of extracts was lower than doxorubicin. Campesterol, ferruginol, stigmasterol, cis-13-octadecenoic acid methyl esters, and methyl palmitate were predicted to play a role in the antioxidant activity and cytotoxicity of RC, RM, and LM. RC, RM, and LM possess the potential for development as anticancer agents. Moreover, RM shows promise as an antioxidant due to its notable radical scavenging activity. Further research is required to explore the cytotoxic effects of RC, RM, and LM on normal cells and to assess their toxicity in experimental animals. 
Efektivitas Gel Ekstrak Etanol 70% Daun Anggrek Oncidium (Oncidium Aliceara alice) terhadap Penyembuhan Luka Kulit Dorsum Tikus Sprague Dawley Onibala, Michele Liony Maria; Suntadi, Maria Ariska Happy; Wiadji, Jovitta Theachrysilla; Oktaviani, Yustina Wanda Dwi; Gunawan, Yohanes Candra; Widhiastuti, Stefani Santi
Jurnal Kefarmasian Indonesia VOLUME 13, NOMOR 1, FEBRUARI 2023
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jki.v13i1.6211

Abstract

Wounds are tissue damage that is generally caused by physical contact. Wound healing is a complex mechanism involving biochemical and biocellular processes. Various therapies can be done to speed up the wound healing process, one of which is treatment using natural ingredients. One of the plants that have the potential to heal wounds is Oncidium Aliceara alice. The purpose of this study was to determine the phytochemical content of Oncidium Aliceara alice and its activity in wound healing when it is formulated in a gel form. This research is experimental research with extraction stages, phytochemical assay, gel formulations, quality test, and wound healing activity tests in animals. The gel formulation was made with various concentrations of Oncidium Aliceara alice extract 2.5%, 5% and 10%. Male Sprague Dawley rats were given an incision wound on the dorsum of the longitudinal axis along 3 cm. Wound care by administering topical Bioplacenton as a positive control, base gel as a negative control, and gel extract concentration variants according to each treatment group. Based on the research, the results of qualitative phytochemical tests showed that the 70% ethanol extract of Oncidium Aliceara alice contains flavonoids (catechins), tannins, steroids, and saponins. The best results were seen on the 7th day of observation for the 10% gel extract treatment group with more perfect signs of wound closure than the bioplacenton treatment.
Co-Authors Abdurahman Laqif Abdurahman Laqif Ahmad Hamim Sadewa Alvina, Devi Arsiningtyas, Ines Septi Atmodjo, Patricius Kianto Bernadia Branitamahisi Bernadia Branitamahisi Bernardus Boy Rahardjo Sidharta Boy Rahardjo Sidharta Demas Bayu Handika Dewi Retnaningati Exsyupransia Mursyanti Exsyupransia Mursyanti, Exsyupransia Gunawan, Yohanes Candra Hida, Fitriana Nur Laissya Ida Ayu Preharsini Ida Ayu Preharsini Kusuma Ines Septi Arsiningtyas Jovitta Theachrysilla Wiadji Mahardhika, I Wayan Swarautama Maria Ariska Happy Suntadi Meisy Michele Liony Maria Onibala Nor Sri Inayati Nor Sri Inayati Oktaviani, Yustina Wanda Dwi Onibala, Michele Liony Maria P Kianto Atmodjo Pantalea Edeilweiss Vitara Paramitha, Prizka Kezia Patricius Kianto Atmodjo Sendy Junedi Setiawan, Pamela Felita Sidharta, Bernardus Boy Rahardjo Sofia Mubarika Haryana Subandriyo, Jessica Rieko Suntadi, Maria Ariska Happy To'bungan, Nelsiani Wiadji, Jovitta Theachrysilla Yohanes Candra Gunawan Yustina Wanda Dwi Oktaviani