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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Journal of Tropical Biodiversity and Biotechnology BERITA BIOLOGI Jurnal Atma Inovasia Jurnal Kefarmasian Indonesia
Widhiastuti, Stefani Santi
Universitas Atma Jaya Yogyakarta
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Environmental Science Immunology & microbiology Medicine & Pharmacology Social Sciences Other

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Phytochemical Properties, Antioxidant, and Cytotoxicity Activity of Knobweed (Hyptis capitata) from South Sulawesi, Indonesia To'bungan, Nelsiani; Widhiastuti, Stefani Santi; Hida, Fitriana Nur Laissya; Mahardhika, I Wayan Swarautama
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 3 (2024): September
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.90976

Abstract

Hyptis capitata Jacq. known as Sumambu plants in Sulawesi, has phytopharmaceutical importance. H. capitata extracts were evaluated for their phytochemical properties, antioxidant activity, and cytotoxicity. Using the maceration yielded five types of extracts: root chloroform (RC), root methanol (RM), leaf chloroform (LC), leaf methanol (LM), and leaf ethanol (LE). Phytochemical properties were identified by qualifying procedure and digital image analysis for quantifying Red-Green-Blue (RGB) percentage and hex colour code. 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay was used to determine the half-maximal inhibitory concentration (IC50). Cytotoxicity screening of each extract was performed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay against HeLa and 4T1 cells. Gas Chromatography-Mass Spectrometry (GC-MS) assay was used to identify the phytochemical compounds of the extracts with the most promising potential. Alkaloids were the major constituents of the phytochemicals of RC, RM, LE, LC, and LM. RM and LM have potency and weak free radical scavenging activities, with IC50 value 31.08 and 58.03 µg/mL, respectively. The IC50 of RC and RM against HeLa cells were 84.21 ± 0.63 and 172.10 ± 02.90 µg/mL, respectively. Meanwhile, the cytotoxicity of RC and RM against 4T1 cells were 86.42 ± 0.80 and 182.82 ± 7.00 µg/mL, respectively. It means RC and RM exhibit a moderate level of cytotoxicity in both HeLa and 4T1 cells. LM shows moderate cytotoxicity, but it is limited to 4T1 cells with an IC50 value of 181.86 ± 12.68 µg/mL. The cytotoxicity level of extracts was lower than doxorubicin. Campesterol, ferruginol, stigmasterol, cis-13-octadecenoic acid methyl esters, and methyl palmitate were predicted to play a role in the antioxidant activity and cytotoxicity of RC, RM, and LM. RC, RM, and LM possess the potential for development as anticancer agents. Moreover, RM shows promise as an antioxidant due to its notable radical scavenging activity. Further research is required to explore the cytotoxic effects of RC, RM, and LM on normal cells and to assess their toxicity in experimental animals. 
Efektivitas Gel Ekstrak Etanol 70% Daun Anggrek Oncidium (Oncidium Aliceara alice) terhadap Penyembuhan Luka Kulit Dorsum Tikus Sprague Dawley Onibala, Michele Liony Maria; Suntadi, Maria Ariska Happy; Wiadji, Jovitta Theachrysilla; Oktaviani, Yustina Wanda Dwi; Gunawan, Yohanes Candra; Widhiastuti, Stefani Santi
Jurnal Kefarmasian Indonesia VOLUME 13, NOMOR 1, FEBRUARI 2023
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jki.v13i1.6211

Abstract

Wounds are tissue damage that is generally caused by physical contact. Wound healing is a complex mechanism involving biochemical and biocellular processes. Various therapies can be done to speed up the wound healing process, one of which is treatment using natural ingredients. One of the plants that have the potential to heal wounds is Oncidium Aliceara alice. The purpose of this study was to determine the phytochemical content of Oncidium Aliceara alice and its activity in wound healing when it is formulated in a gel form. This research is experimental research with extraction stages, phytochemical assay, gel formulations, quality test, and wound healing activity tests in animals. The gel formulation was made with various concentrations of Oncidium Aliceara alice extract 2.5%, 5% and 10%. Male Sprague Dawley rats were given an incision wound on the dorsum of the longitudinal axis along 3 cm. Wound care by administering topical Bioplacenton as a positive control, base gel as a negative control, and gel extract concentration variants according to each treatment group. Based on the research, the results of qualitative phytochemical tests showed that the 70% ethanol extract of Oncidium Aliceara alice contains flavonoids (catechins), tannins, steroids, and saponins. The best results were seen on the 7th day of observation for the 10% gel extract treatment group with more perfect signs of wound closure than the bioplacenton treatment.
Pelatihan Guru Biologi dan Siswa SD-SMA/SMK di Pontianak untuk Meningkatkan Pengalaman Belajar di Bidang Bioteknologi Mursyanti, Exsyupransia; Stefani Santi Widhiastuti; Dewi Retnaningati
Jurnal Atma Inovasia Vol. 2 No. 1 (2022)
Publisher : Lembaga Penelitian dan Pengabdian pada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/jai.v2i1.4624

Abstract

 Abstract — Pontianak is the center of education in the province of West Kalimantan. In the implementation of education and learning, the interaction between competent teachers and active students can improve the quality of education. Therefore, a service activity was made that aims to ensure quality educational services in accordance with the times. This service activity is entitled Training and Assistance for Biology Teachers and Elementary-SMA/SMK Students at Karya Yosep Elementary School, St. Peter's Middle/High School, St. Mary's Vocational School with the theme Biotechnology and its Applications for the Welfare of Human Life. The method used is contextual learning which is packaged with a webinar model which includes 2 sessions, namely the delivery of material and discussion sessions. This activity is divided into 3 stages, namely the preparation, implementation, and evaluation stages. Overall, the activity went smoothly. This activity can increase the knowledge of Biology teachers and students in partner schools about the field of biotechnology, and participants can practice and apply the material received in everyday life. More than 85% of the participants from the group of teachers, elementary-junior high school students, and high school/vocational school students thought that the material provided was in accordance with the needs.Keywords — training, teachers, students, biotechnology.
Pelatihan dan Pendampingan Prapanen, Panen dan Pascapanen kepada Petani Jahe Area Paroki Boro, Kabupaten Kulon Progo junedi, sendy; Ines Septi Arsiningtyas; P Kianto Atmodjo; Exsyupransia Mursyanti; Boy Rahardjo Sidharta; Stefani Santi Widhiastuti
Jurnal Atma Inovasia Vol. 3 No. 5 (2023)
Publisher : Lembaga Penelitian dan Pengabdian pada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/jai.v3i5.7594

Abstract

Paroki Santa Theresia Lisieux (STL) Boro terletak pada area pengembangan pariwisata sehingga berpotensi menjadi desa wisata dengan amenitas khas berupa jahe emprit. Produksi jahe pada area ini memiliki keterbatasan kuantitas, kontinuitas, dan kualitas karena minimnya pengetahuan dan ketrampilan petani dalam penanaman dan pemeliharaan tanaman serta pengolahan rimpang jahe menjadi produk jadi. Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta memberikan pelatihan dan pendampingan untuk memperbaiki pengetahuan dan ketrampilan petani jahe di area Paroki STL Boro. Pelatihan dan pendampingan dibagi menjadi tujuh kali pertemuan yang terdiri dari sosialisasi dan evaluasi pengetahuan dalam bentuk kuesioner, dilanjutkan dengan pelatihan tentang prapanen, panen, pascapanen primer dan sekunder, serta yang terakhir adalah evaluasi berupa diskusi dan kuesioner. Topik pertemuan pascapanen sekunder terdiri dari pembuatan produk kesehatan (serbuk jahe instan) dan produk kosmetik (lulur jahe). Hasil analisis data kuesioner secara semikuantitatif menunjukkan terjadi peningkatan pengetahuan petani sebesar 75% antara sebelum dan sesudah pelatihan. Dengan adanya pelatihan, petani di Paroki STL Boro mampu menghasilkan produk jahe instan yang telah dipasarkan secara terbatas.
Pelatihan Pengolahan Produk dari Bunga Mawar pada Masyarakat di Kawasan Sapuangin, Merapi, Klaten Widhiastuti, Stefani Santi; Mursyanti, Exsyupransia; Sidharta, Bernardus Boy Rahardjo; Atmodjo, Patricius Kianto; Junedi, Sendy; Arsiningtyas, Ines Septi; To'bungan, Nelsiani
Jurnal Atma Inovasia Vol. 3 No. 6 (2023)
Publisher : Lembaga Penelitian dan Pengabdian pada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/jai.v3i6.8082

Abstract

Roses are one of the commodities in the Sapuangin area, Pemalang, Klaten. The fluctuating selling price and short shelf life make fresh roses only a secondary product. Therefore, a training is needed to process rose flowers into products with high economic value and longer shelf life. This community service aims to enhance the community's knowledge in processing various products from rose flowers. The activities include survey, socialization, training implementation, and evaluation. The training consists of 7 topics from June to July 2023, where participants receive oral explanations and practical demonstrations of the materials provided. The evaluation includes assessing the participants' knowledge through questionnaires given at the beginning and end of the community service, as well as satisfaction evaluation. The entire community service runs smoothly, with participants able to practice making raw materials, solid soap, bath salts, aromatherapy candles, rosewater, and hand sanitizer. Participants also learn about the different types of roses. The average score of the questionnaires from 15 respondents before the training was 21.33 ± 2.61, increasing by 53.49% to 32.74 ± 3.90 from 19 respondents. 68.42% of participants expressed very satisfied, and 31.58% expressed satisfied with participating in the community service activities. In conclusion, the community service has improved the knowledge and skills of the Sapuangin Village community in creating products from rose flowers that have higher economic value and longer shelf life. Keywords — roses, soap, candles, hand sanitizer, rosewater   Abstrak— Mawar merupakan salah satu komoditi di kawasan Sapuangin, Pemalang, Klaten. Harga jual yang tidak menentu dan masa simpan pendek menyebabkan mawar segar hanya menjadi produk sampingan. Oleh karena itu diperlukan kegiatan pelatihan pengolahan bunga mawar menjadi produk dengan nilai ekonomis tinggi dan masa simpan panjang. Pengabdian pada masyarakat ini bertujuan untuk meningkatkan pengetahuan masyarakat dalam mengolah bunga mawar menjadi berbagai macam produk. Kegiatan meliputi tahap survey, sosialisasi, pelaksanaan pelatihan, dan evaluasi. Pelatihan terdiri dari 7 materi mulai Juni-Juli 2023, dimana peserta diberi penjelasan secara lisan dan mempraktekkan secara langsung materi yang diberikan. Evaluasi meliputi evaluasi tingkat pengetahuan melalui kuesioner yang diberikan di awal dan di akhir kegiatan pengabdian, serta evaluasi kepuasan. Seluruh kegiatan pengabdian berjalan lancar, peserta dapat mempraktekkan pembuatan simplisia, sabun padat, garam mandi, lilin aromaterapi, air mawar dan handsanitizer. Peserta juga mengetahui jenis-jenis bunga mawar. Nilai rata-rata kuesioner dari 15 responden sebelum pelatihan adalah 21,33 ± 2,61, meningkat sebesar 53,49% menjadi 32,74 ± 3,90 dari 19 responden. 68,42% peserta menyatakan sangat puas dan 31,58% menyatakan puas mengikuti kegiatan pengabdian. Dapat disimpulkan bahwa kegiatan pengabdian dapat meningkatkan pengetahuan dan kemampuan masyarakat Desa Sapuangin dalam membuat produk dari bunga mawar yang memiliki nilai ekonomis lebih tinggi dengan masa simpan panjang.   
Pelatihan propagasi anggrek skala rumah tangga kepada kelompok wisata di kawasan Sapuangin, Merapi, Klaten Mursyanti, Exsyupransia; Nelsiani To’bungan; Pantalea Edeilweiss Vitara; Bernardus Boy Rahardjo Sidharta; Patricius Kianto Atmodjo; Sendy Junedi; Stefani Santi Widhiastuti
Jurnal Atma Inovasia Vol. 4 No. 3 (2024)
Publisher : Lembaga Penelitian dan Pengabdian pada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/jai.v4i3.8912

Abstract

Sapuangin area is one of supporting areas for Mount Merapi National Park (TNGM). The endemic Merapi orchid is widely cultivated by people in the Sapuangin, using conventional cultivation. Sapuangin’s youth, who are members of a tourism group (POKWIS) have never received training regarding orchid propagation using in vitro culture principles. This is important to support orchid conservation activities carried out by POKWIS in the Sapuangin greenhouse. Based on requests from the community, lecturers and educational laboratory staff concentrating on Technobio-Industrial Studies, Faculty of Biotechnology, Universitas Atma JayaYogyakarta, provided a series of training on in vitro orchid culture, for household scale. This activity is expected to provide knowledge and skills regarding orchid cultivation techniques for the community. The activity was held on 12-20 December 2023. The training activity was divided into several topics, namely introduction to orchid types and their uses, principles of in vitro orchid culture, introduction to tools, methods for sterilizing and sowing orchid seeds, pollination/pollination, overplanting, and acclimatization. The training carried out can provide additional knowledge for the community regarding in vitro orchid cultivation techniques. The implementation of the training was considered very useful and by community needs, the training resource was competent, the implementation time was by the plan, and was supported by complete facilities and infrastructure. The community hopes for ongoing guidance and assistance regarding orchid propagation in the future.
Salep Ektrak Daun Jamblang (Syzygium cumini) sebagai Penghambat Bakteri Propionibacterium acnes Penyebab Jerawat Setiawan, Pamela Felita; Alvina, Devi; Subandriyo, Jessica Rieko; Meisy; Paramitha, Prizka Kezia; Widhiastuti, Stefani Santi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 1 (2024): February 2024
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i1.6552

Abstract

Jerawat merupakan gangguan pada permukaan tubuh yang umumnya sering muncul pada wajah. Bakteri Propionibacterium acnes merupakan salah satu bakteri penyebab jerawat. Penggunaan antibiotik memiliki efek samping pada resiko hipersensitivitas atau alergi, sehingga penggunaan bahan alami sebagai obat herbal menjadi alternatif pengobatan yang baik. Salah satu bahan alami dengan potensi antibakteri yang dapat menghambat pertumbuhan bakteri P. acnes adalah daun tanaman jamblang dengan kandungan antibakteri. Metode pengujian daun jamblang berupa pembuatan ekstrak daun jamblang, skrining fitokimia ekstrak, formulasi salep, uji zona hambat dan uji stabilitas produk salep. Tujuan dari penelitian ini adalah untuk mengetahui kemampuan salep ekstrak daun jamblang dalam menghambat bakteri penyebab jerawat P. acnes, serta sifat fisik dan stabilitasnya selama penyimpanan. Hasil yang diperoleh menunjukkan bahwa ekstrak daun jamblang memiliki kandungan flavonoid, triterpenoid, tannin, saponin, dan alkaloid. Hasil terbaik pada uji zona hambat ekstrak dan salep tampak pada konsentrasi 10% yang memiliki aktivitas antibakteri paling baik terhadap P. acnes dengan diameter zona hambat 9,17 mm, sedangkan hasil uji stabilitas terbaik pada salep konsentrasi 15% dengan pH 6,5 ± 0,5, daya sebar 3,17 ± 0,26, daya lekat >2 menit. 
Co-Authors Abdurahman Laqif Abdurahman Laqif Ahmad Hamim Sadewa Alvina, Devi Arsiningtyas, Ines Septi Atmodjo, Patricius Kianto Bernadia Branitamahisi Bernadia Branitamahisi Bernardus Boy Rahardjo Sidharta Boy Rahardjo Sidharta Demas Bayu Handika Dewi Retnaningati Exsyupransia Mursyanti Exsyupransia Mursyanti, Exsyupransia Gunawan, Yohanes Candra Hida, Fitriana Nur Laissya Ida Ayu Preharsini Ida Ayu Preharsini Kusuma Ines Septi Arsiningtyas Jovitta Theachrysilla Wiadji Mahardhika, I Wayan Swarautama Maria Ariska Happy Suntadi Meisy Michele Liony Maria Onibala Nor Sri Inayati Nor Sri Inayati Oktaviani, Yustina Wanda Dwi Onibala, Michele Liony Maria P Kianto Atmodjo Pantalea Edeilweiss Vitara Paramitha, Prizka Kezia Patricius Kianto Atmodjo Sendy Junedi Setiawan, Pamela Felita Sidharta, Bernardus Boy Rahardjo Sofia Mubarika Haryana Subandriyo, Jessica Rieko Suntadi, Maria Ariska Happy To'bungan, Nelsiani Wiadji, Jovitta Theachrysilla Yohanes Candra Gunawan Yustina Wanda Dwi Oktaviani