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All Journal Klinikal Sains (Jurnal Analis Kesehatan) Proceedings of the International Conference on Nursing and Health Sciences Indonesian Journal on Medical Science (IJMS) Prosiding Seminar Informasi Kesehatan Nasional Indonesian Journal of Community Empowerment (IJCE)
Prabandari, Anggraeni Sih
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Nursing Public Health

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Isolasi dan Identifikasi Jamur Penyebab Onikomikosis pada Petani di Kabupaten Sukoharjo Jawa Tengah Prabandari, Anggraeni Sih; Pramonodjati, Fredericus; Sari, Ajeng Novita; Cahyaningtias, Agatha Dinda Ayu
Indonesian Journal on Medical Science Vol 11 No 1 (2024): IJMS 2024
Publisher : Unit Penelitian dan Pengabdian Masyarakat Politeknik Kesehatan Bhakti Mulial

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55181/ijms.v11i1.470

Abstract

Farmers are susceptible to dermatophyte infection because of their daily contact with the soil which is the habitat of mold. Onychomycosis (tinea unguium) is a disease of the nails and toenails caused by dermatophytes. Although it does not cause death, this disease can reduce the productivity of sufferers. Personal hygiene, the use of personal protective equipment and the long time of being a farmer, are closely related to the incidence of Onychomycosis. This study aims to determine the species of mold that causes onychomycosis. This type of research is descriptive with a cross sectional approach. The respondents of the study amounted to 24 rice farmers in Mayang Village, Gatak District, Sukoharjo Regency. Data in this study were obtained from interviews, observations and nail examinations. Onychomycosis is seen based on changes in structure and color in the fingernails and toenails. The infected nail is removed and examined microscopically. The specimen was then cultured on Saboraoud Dextrose Agar (SDA) media. Growing colonies are identified macroscopically by looking at the morphology and characteristics of the colony and examined microscopically by staining Lactophenol Cotton Blue (LCBP). The results of the study found two types of dermatophyte and non-dermatophyte fungi. Onychomycosis infection is dominated by dermatophyte fungi with a prevalence of 63% (15/24), while the rate of non-dermatophyte fungal infection is 37% (9/24). Dermatophyte species that infected Trichopyton rubrum (33%) and Trichopyton mentagophytes (29%). While non-dermatophyte fungi found are Aspergilus fumigatus (17%) and Aspergilus niger (21%).
Kadar Timbal (Pb) dalam Darah Sukarelawan Pengatur Lalu Lintas (Supeltas) di Kota Surakarta Ditinjau dari Usia, Lama Kerja dan Kebiasaan Merokok Prabandari, Anggraeni Sih; Sari, Ajeng Novita; Pramonodjati, Fredericus; Wulandari, Tata
Indonesian Journal on Medical Science Vol 11 No 2 (2024): IJMS 2024
Publisher : Unit Penelitian dan Pengabdian Masyarakat Politeknik Kesehatan Bhakti Mulial

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70050/ijms.v11i2.488

Abstract

Lead (Pb) can enter the blood through inhalation. Traffic management volunteers (supeltas) has high risk of Pb exposure because they are often exposed to vehicle smoke containing Pb. In addition to occupational risks, high blood Pb levels can be affected by age, duration of exposure and smoking habits. This study aims to determine the level of lead in the blood of supeltas based on age, working period and smoking habits. It was descriptive analytical research with a cross sectional design. Sixteen men were involved in this research. Blood was drawn from their vein and added with EDTA anticoagulant. Pb levels in the blood were analyzed by the Flame Atomic Absorption Spectrophotometer (FAAS). The Spearman test was used to determine the strength of correlation of age, length of work and smoking habits on Pb levels. The average Pb level in the blood of the supeltas was 117.45 ?g/dL. The lowest level of Pb was found in the P10 and P5 samples, which was 3,7 ?g/dL, while the highest level in P16 was 668,7 ?g/dL. About 75% (12/16) of supeltas had high Pb blood levels. The results of Spearman's analysis showed that there was no significant relationship between blood lead levels and age (p-value 0,507>0,05), working age (p-value 0.976>0,05) and smoking habits (p-value 0,543>0,05).
Anal Swab Examination of Salmonella in Typhoid Patients after Antibiotics Treatment Prabandari, Anggraeni Sih; Ismail, Nor Isnida; Utami, Nataly Ayu
Proceedings of the International Conference on Nursing and Health Sciences Vol 5 No 1 (2024): January-June 2024
Publisher : Global Health Science Group

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/picnhs.v5i1.3887

Abstract

Thypoid is an endemic infectious disease in several countries caused by Salmonella thypi and Salmonella parathypi bacteria. These bacteria can be found in the blood, feces and urine of sufferers. Ciprofloxacin, Amoxicillin, Chloramphenicol and Ceftriaxone are commonly prescribed antibiotics in the treatment of thypoid. A stool examination to find the bacteria that causes thypoid can be used to evaluate the success of treatment and the patient's recovery rate. S. thypi Ag Rapid Test is an easy and fast method to detect the presence of thypoid bacteria. Objective: This study aims to detect the presence of thypoid bacteria in the feces of patients who receive antibiotic treatment on days 5, 10 and 15. Method: It was an observation research with a cross sectional approach. Samples in the form of anal swabs from 36 thypoid fever patients were taken on days 5, 10 and 15 after receiving antibiotic treatment. The examination uses S. thypi Ag Rapid Test. Results: A total of 27.8% of the samples showed positive results on the 5th day of examination, 22.2% were positive on the 10th day of examination and 11.1% of the samples were positive on the 15th day of the examination regardless of the type of antibiotic given. Conclusion: There was a decrease in the number of positive samples from day 5 to day 15, indicating improvement or recovery in some samples.
Edukasi Pencegahan Anemia dan Pemeriksaan Kadar Hemoglobin pada Remaja Putri di Kelurahan Gedangan Kecamatan Grogol Kabupaten Sukoharjo Prabandari, Anggraeni Sih; Novita Sari, Ajeng; Fredericus Pramonodjati; Anastasia Indarwati Utomo; Pradita Yudi Saputro
INDONESIAN JOURNAL OF COMMUNITY EMPOWERMENT (IJCE) Vol. 5 No. 2 (2023): Indonesian Journal of Community Empowerment November 2023
Publisher : UNIVERSITAS NGUDI WALUYO

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35473/ijce.v5i2.2621

Abstract

Growth spurt, lack of consumption of animal protein sources, consuming tea/coffee along with main meals and menstruation every month can be causes of anaemia in female adolescent. The incidence of anaemia in female adolescent in Sukoharjo Regency is 28.08% and it is predicted that it will continue to increase if preventive measures are not taken, one of which is by education on anaemia prevention. Therefore, hemoglobin (Hb) levels have been checked to determine the status of anaemia in female adolescent in Gedangan Village, Grogol District, Sukoharjo Regency. In addition to measuring Hb levels, education is also carried out in the form of scientific lectures and discussions. The number of participants in this activity was 23 people. Based on the results of Hb measurement, data were obtained as many as 20% suffer from anaemia and 80% are not anaemic. The knowledge level of female adolescent was increased after being educated, with the result that 82.6% had knowledge at a "good" level.   ABSTRAK                 Lonjakan pertumbuhan, kurangnya konsumsi sumber protein hewani, mengkonsumsi teh/kopi bersamaan dengan makanan utama dan menstruasi setiap bulan dapat menjadi penyebab anemia pada remaja putri. Angka kejadian anemia pada remaja putri di Kabupaten Sukoharjo sebesar 28,08% dan diperkirakan akan terus meningkat jika tidak dilakukan tindakan preventif, salah satunya dengan edukasi pencegahan anemia. Oleh karena itu, telah dilakukan pemeriksaan kadar hemoglobin (Hb) untuk mengetahui status anemia pada remaja putri di Kelurahan Gedangan Kecamatan Grogol Kabupaten Sukoharjo. Selain pengukuran kadar Hb, dilakukan juga edukasi dalam bentuk ceramah ilmiah dan diskusi. Jumlah partisipan dalam kegiatan ini sebanyak 23 orang. Berdasarkan hasil pengukuran Hb diperoleh data sebanyak 20% menderita anemia dan 80% tidak anemia. Tingkat pengetahuan remaja putri meningkat setelah diberikan edukasi, dengan hasil sebanyak 82,6 % memiliki pengetahuan pada tingkat “baik”.
The PERBANDINGAN EFEKTIVITAS AIR PERASAN JERUK NIPIS (Citrus aurantifolia) DAN BELIMBING WULUH (Averrhoa blimbi L) DALAM PEMERIKSAAN HITUNG JUMLAH LEUKOSIT Prabandari, Anggraeni Sih; Sari, Ajeng Novita; Darwati, Mening Sri
Klinikal Sains : Jurnal Analis Kesehatan Vol 12 No 1 (2024): Juni
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i1.3342

Abstract

Juice of lime (Citrus aurantifolia) and starfruit (Averrhoa blimbi L) contain citric acid which has the same characteristics as glacial acetic acid in Turk's solution. This study was carried out to determine the potential of them as a substitute for glacial acetic acid in the leukocyte count and compare it with Turk's solution as a standard. We used three variations of concentration, namely 1%, 2% and 3%. The specimen used was venous blood with EDTA anticoagulant which was examined using an improved Neubauer counting chamber. The result showed average number of leukocytes calculated using a solution of star fruit and lime at a concentration of 1% was 6287 cells/mm3 vs 4537 cells/mm3, a concentration of 2% was 3525 cells/mm3 vs 3387 cells/mm3 and at a concentration of 3% was 2775 cells/mm3 vs 3225 cells/mm3. The number of leukocytes in the control (Turk's solution) was 7050 cells/mm3. Starfruit juices with a concentration of 1% gave the results closest to the control. Based on the results of the one-way ANOVA test, the sig value was 0.000 (p<0.05) and the independent t-test showed a value (p>0.05), which means that there was no difference in the number of leukocytes examined with Turk's solution and modified starfruit solution. We concluded, starfruit juices with a concentration of 1% was recommended as a substitute for Turk's solution than lime juice.
PEMANFAATAN EKSTRAK UMBI TANAMAN BIT (Beta vulgaris L.) PADA PEMERIKSAAN TELUR CACING METODE KATO KATZ Suyono, Agnes Regita Pramesthi; Prabandari, Anggraeni Sih; Sari, Ajeng Novita
Prosiding Seminar Informasi Kesehatan Nasional 2024: SIKesNas 2024
Publisher : Fakultas Ilmu Kesehatan Universitas Duta Bangsa Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47701/sikenas.vi.3885

Abstract

Latar Belakang : Prevalensi kecacingan di Indonesia tergolong masih tinggi. Spesies yang sering menginfeksi manusia adalah soil transmitted helminths, diantaranya Ascaris lumbricoides, Trichuris trichiura, dan hookworm. Salah satu metode yang digunakan dalam mendiagnosis kecacingan adalah metode Kato-katz menggunakan pewarna malachite green, namun pewarna ini bersifat toksik sehingga perlu dilakukan subsitusi menggunakan pewarna alami. Umbi bit (Beta vulgaris L.) sering dijadikan sebagai pewarna karena mengandung betasianin. Tujuan: Untuk mengetahui kemampuan larutan umbi tanaman bit dalam mewarnai latar belakang dan telur cacing pada pemeriksaan feses semi kuantitatif metode Kato-Katz. Metode : Jenis penelitian adalah eksperimental menggunakan 3 feses anak yang positif telur Trichuris trichiura. Konsentrasi larutan pewarna umbi bit yang digunakan adalah 50% dan 95% yang diamati dengan mengukur kontras dan kejelasan latar belakang. Hasil : Rerata hasil scoring yang paling tinggi yaitu malachite green dengan 2.85 untuk kontras dan 2.67 untuk kejelasan latar belakang. Konsentrasi yang paling optimal yaitu konsentrasi 95% yang dapat terlihat dari nilai rata-rata skoring yang mendekati kontrol (malachite green) yaitu untuk kontras 2.80 dan untuk kejelasan latar belakang 2.81, sedangkan rata-rata konsentrasi 50% yaitu untuk kontras 1.40 dan untuk kejelasan latar belakang 1.41. Pada uji beda Oneway ANOVA tidak menunjukkan adanya perbedaan kontras dan kejelasan latar belakang antara larutan konsentrasi 95% dengan malachite green (nilai sign >0.005). Simpulan : Larutan konsentrasi umbi bit 95% efektif dapat digunakan sebagai pengganti malachite green dalam pemeriksaan feses metode Kato-katz (pvalue kontras 0.097>0.005 dan pvalue kejelasan latar belakang 0.128>0.005).
PERBEDAAN HASIL PEMERIKSAAN DARAH RUTIN YANG DISIMPAN PADA SUHU 4-8ï‚°C DITUNDA 1 JAM DAN 3 JAM Wijaya, Silvia Monika; Pramonodjati, F. Pramonodjati; Prabandari, Anggraeni Sih; Sari, Ajeng Novita
Prosiding Seminar Informasi Kesehatan Nasional 2024: SIKesNas 2024
Publisher : Fakultas Ilmu Kesehatan Universitas Duta Bangsa Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47701/sikenas.vi.3887

Abstract

Latar Belakang: Pemeriksaan darah rutin dilakukan untuk membantu diagnosa penyakit dan mengetahui adanya kelainan. Pemeriksaan ini meliputi hemoglobin, hematokrit, laju endap darah, hitung sel leukosit, hitung sel eritrosit, dan hitung sel trombosit. Pemeriksaan terkadang bisa tertunda karena saluran listrik yang rusak, alat mengalami gangguan, stok reagen habis dan keterbatasan ATLM. Pada kondisi tersebut, sampel dapat disimpan di lemari pendingin. Tujuan Penelitian: Mengetahui perbedaan hasil pemeriksaan darah rutin pada sampel yang disimpan suhu 4-8℃ dan ditunda pemeriksaannya selama 1 dan 3 jam dibandingkan dengan spesimen yang diperiksa segera. Metode Penelitian: Jenis penelitian adalah eksperimental dengan jumlah sampel sebanyak 10 darah EDTA. Pemeriksaan dilakukan secara otomatis menggunakan hematology analyzer. Uji statistik yang digunakan adalah uji normalitas Shapiro Wilk dan uji beda oneway ANOVA. Hasil Penelitian: Kadar hemoglobin dan hematokrit relatif stabil terhadap pengaruh waktu dan suhu dibandingkan parameter lainnya. Leukosit, trombosit dan laju endap darah cenderung meningkat pada spesimen yang disimpan pada suhu dingin, sebaliknya kadar eritrosit justru menurun. Hasil uji beda oneway ANOVA menunjukkan tidak ada perbedaan hasil antara spesimen yang diperiksa langsung dan penundaan pemeriksaan selama 1 dan 3 jam pada spesimen yang disimpan pada suhu dingin (nilai sig seluruh parameter >0,05). Simpulan: Spesimen pemeriksaan darah rutin yang disimpan pada suhu 4-8℃ dengan penundaan pemeriksaan 1 dan 3 jam memberikan hasil yang sama baik dengan spesimen yang langsung diperiksa.
Co-Authors Anastasia Indarwati Utomo Cahyaningtias, Agatha Dinda Ayu Darwati, Mening Sri Fredericus Pramonodjati Ismail, Nor Isnida Novita Sari, Ajeng Pradita Yudi Saputro Pramonodjati, F. Pramonodjati Pramonodjati, Fredericus Sari, Ajeng Novita Suyono, Agnes Regita Pramesthi Utami, Nataly Ayu Wijaya, Silvia Monika Wulandari, Tata