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All Journal Jurnal Hortikultura Indonesia (JHI) AGRIVITA, Journal of Agricultural Science Journal of Tropical Biodiversity and Biotechnology
Rahayu, Resa Sri
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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In Vitro Seed Germination and Shoot Growth of Nepenthes jamban Chi. C. Lee, Hernawati & Akhriadi, A Unique Pitcher Plant from Indonesia Prawestri, Apriliana Dyah; Rahayu, Resa Sri; Kurniajati, Wulan Septiningtyas; Sunardi, Sunardi; Mansur, Muhammad
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 2 (2024): June
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.87674

Abstract

The study to optimize in vitro propagation of the Indonesian native and critically endangered species, Nepenthes jamban, in order to support the ex-situ conservation efforts has been done.  Using Murashige and Skoog (MS) as a basal media, disinfected seeds of N. jamban were germinated on five types of germination media, viz. ¼ MS, ½ MS, MS, ¼ MS+benzyl adenine (BA)+Biotin and MS+BA+Biotin. Afterwards, in vitro shoots with 6-7 leaves were inoculated on growing media, i.e., ¼ MS, ¼ MS 60 (3:1) (MS modification with a higher concentration of nitrogen), and ¼ MS+naphtalene acetic acid (NAA)+BA. The results showed that the germination of N. jamban seeds was slow, indicated by the percentage of germination being less than 20% after 6 months of being planted on germination media. The highest percentage of germination was after the 6th month and the greatest pitcher development at the 10th month were obtained on ¼ MS medium. Furthermore, shoot growth and pitchers development consistently increased for 12 months in ¼ MS 60 (3:1) medium while other media resulted in a decrease in pitcher formation. It seemed that low concentrations of nutrient in the medium proved to be more effective to induce in vitro seed germination and enhance shoot growth which was also supported by higher nitrogen (nitrate) concentration in the medium. This study provides information that supports ex situ conservation action of native and critically endangered Nepenthes species from Indonesia. 
Bibliometric Analysis, Primer Design, and AcFT1 Expression of Shallots under In Vitro Multiplication Rantau, Deritha Ellfy; Noorohmah, Siti; Rahayu, Resa Sri; Syahid, Sitti Fatimah; Hapsari, Betalini Widhi; Wulandari, Dyah Retno; Raihan, Eldrian Daffa; Haz, Aufa Rizqia; Kumala, Ajeng Putri; Yuliawati, Yuliawati; Desriani, Desriani
AGRIVITA Journal of Agricultural Science Vol 47, No 1 (2025)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v47i1.4548

Abstract

The use of botanical seeds of shallot as planting materials is more effective than bulbs. However, the characteristics of plants are not ‘true to type’. Bibliometric analysis can identify areas that have been under- explored. Research on biomolecule compounds and gene expression is needed to support biomarker-based detection technology to predict plant productivity early.  This research aims to study the expression of the AcFT1 gene to compare two shallot plantlets with different responses (non-multiplied and multiplied). The AcFT1 gene was identified by bibliometric analysis. GapC2 (group of housekeeping genes) was selected as an internal control gene. The primer designed result were: AcFT1-F: 5’GCGAGAAACCGTCTGCTATGA3’; AcFT1-R: 5’GCAACTGGA GACCCAAGGTT3’; GapC2-F: 5’GCTGCACAACCAACTGCTTA3’; GapC2-R:  5’CCAGTGCTGCTAGGAATGAT3’. The RNA from micro bulb of shallot was then extracted and converted into cDNA with RT-PCR process. Based on the best-optimized PCR annealing temperature (55.2oC), the GapC2 and AcFT1 genes were expressed at the same thickness for both phenotypes, indicating the same level of expression in both micro bulbs. Further, this showed that AcFT1 cannot be used for comparative multiplication studies, this gene is more related to the bulb formation rather than the multiplication process.
Appropriate Duration of Drought Stress for Madura Tangerine Flower Induction: Appropriate Duration of Drought Stress for Madura Tangerine Flower Induction Rahayu, Resa Sri; Poerwanto, Roedhy; Efendi, Darda; Widodo, Winarso Drajad
Jurnal Hortikultura Indonesia (JHI) Vol. 11 No. 2 (2020): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.11.2.82-90

Abstract

Cekaman kekeringan merupakan salah satu teknik yang dilakukan untuk menginduksi bunga jeruk keprok di luar musim dalam upaya memenuhi ketersediaan buah jeruk keprok sepanjang tahun. Durasi cekaman kekeringan yang tepat penting dipelajari untuk menghindari cekaman berat dan mendapatkan hasil bunga yang optimum. Penelitian ini bertujuan menentukan durasi cekaman kekeringan yang tepat untuk menginduksi bunga jeruk keprok dataran rendah varietas Madura. Penelitian dilaksanakan di Kebun Percobaan PKHT-IPB dengan ketinggian ± 300 mdpl dari bulan April-Mei 2019. Percobaan dirancang dengan RAK (Rancangan Acak Kelompok) dengan satu faktor perlakuan yaitu durasi cekaman kekeringan dengan lima taraf: tanpa cekaman kekeringan sebagai kontrol, durasi cekaman kekeringan satu minggu, durasi cekaman kekeringan dua minggu, durasi cekaman kekeringan tiga minggu dan durasi cekaman kekeringan empat minggu. Hasil penelitian menunjukkan bahwa durasi cekaman kekeringan mempengaruhi keberhasilan induksi bunga jeruk keprok Madura. Durasi cekaman kekeringan selama 2, 3 dan 4 minggu dengan nilai kadar air secara berturut-turut 81.81%, 65.21% dan 55.39% dari kapasitas lapang berhasil menginduksi bunga. Bunga muncul pada 3, 2 dan 1 minggu setelah rewatering dan pengairan rutin secara berturut-turut pada perlakuan durasi cekaman 2, 3 dan 4 minggu. Tiga minggu tanpa irigasi merupakan durasi cekaman kekeringan optimum untuk induksi bunga jeruk keprok Madura.
Co-Authors Desriani Desriani Efendi, Darda Hapsari, Betalini Widhi Haz, Aufa Rizqia Kumala, Ajeng Putri Kurniajati, Wulan Septiningtyas MUHAMMAD MANSUR Noorohmah, Siti Prawestri, Apriliana Dyah Raihan, Eldrian Daffa Rantau, Deritha Ellfy Roedhy Poerwanto Sitti Fatimah Syahid Sunardi Sunardi Winarso D. Widodo Wulandari, Dyah Retno Yuliawati