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All Journal Jurnal Bios Logos BERKALA SAINSTEK Jurnal Biologi Tropis JPBIO (Jurnal Pendidikan Biologi) Jurnal Kesehatan InSTEM Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Nihayah, Husnatun
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Physics Public Health Other

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Efektivitas Gum Mimba (Azadirachta indica) sebagai Atraktan Ovitrap Nyamuk Nihayah, Husnatun; Purwatiningsih, Purwatiningsih
Jurnal Kesehatan Vol 14 No 2 (2023): Jurnal Kesehatan
Publisher : Poltekkes Kemenkes Tanjung Karang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jk.v14i2.3661

Abstract

Pemasangan ovitrap atau perangkap telur menjadi salah satu metode surveilans untuk pengendalian nyamuk. Adanya atraktan dengan kandungan senyawa organik yang tinggi dari gum mimba pada ovitrap diharapkan akan meningkatkan oviposisi nyamuk untuk meletakkan telurnya didalam ovitrap tersebut. Tujuan penelitian ini adalah untuk mengetahui efektivitas gum mimba sebagai atraktan ovitrap. Penelitian telah dilaksanakan sejak bulan September hingga November 2022. Larutan gum mimba dibuat dengan melarutkan serbuk gum mimba dengan air panas dengan konsentrasi 2% dan pH7. Sebanyak 130 ovitrap (65 ovitrap air kran, 65 ovitrap larutan gum mimba 2%) telah dipasang di lingkungan Sumbersari serta dilakukan pemanenan telur nyamuk yang terperangkap pada hari ke 8 pemasangan. Telur dan larva yang terperangkap kemudian ditetaskan, diidentifikasi dan analisis dengan uji Mann-Whitney. Hasil uji statistik menunjukkan adanya pengaruh jenis air ovitrap terhadap jumlah nyamuk terperangkap. Ovitrap dengan air kran dinilai lebih efektif karena memiliki nilai OI (61,54%), jumlah telur (3577), ODI (89,42) yang lebih tinggi dibandingkan dengan ovitrap berisi gum mimba (OI 36,92%, Jumlah telur 1463, ODI 60,96). Hasil identifikasi nyamuk menunjukkan spesies Aedes aegypti, Aedes albopictus, Armigeres kesseli, Culex quinquefasciatus. Kesimpulan dari penelitian ini adalah ovitrap dengan air kran lebih efektif dibandingkan dengan ovitrap dengan gum mimba sebagai media.
Morphological Characteristic of Adult Armigeres subalbatus from Sumbersari Jember Nihayah, Husnatun; Purwatiningsih, Purwatiningsih
BERKALA SAINSTEK Vol 11 No 1 (2023)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v11i1.37098

Abstract

Armigeres subalbatus is a type of mosquito that is widely distributed in the world, including Jember Regency, Indonesia. The larval natural habitat is mainly found in trees that can hold water, adult mosquitoes can be found indoors or outdoors. In the field of health, Ar. subalbatus acts as a vector for filariasis, Japanese Encephalitis (JE) and zika. This study aims to identify the morphological characteristics of the Ar. subalbatus from Sumbersari Village, Jember Regency. Although in Indonesia there is no significant evidence of the role of Ar. subalbatus in the health sector, it is important to detect the morphological features of Ar. subalbatus to be able to detect mosquitoes properly. Knowing the exact type of mosquito can provide consideration for appropriate mosquito control methods. The method used is through the installation of ovitrap in November 2022. The ovitrap media is in the form of tap water, the ovitrap is left for 8 days. Ar. mosquitoes were found in the larval stage of the ovitrap. The larvae were brought to the Jember University Faculty of Mathematics and Natural Sciences Zoology Laboratory for hatching and data collection on the morphological characteristics of mosquitoes. The results of this study include the morphological characteristics used for the identification of Ar. subalbatus which includes the shape of the proboscis, flagelomeres on the palps, color of the scutum, border of the scutum, size of the antepronotum, bands on the pleura, prespiracular and postspiracular areas, edges of the mesepimeron, abdomen (tergum, sterna), apex of the femur, venation of the wings, alula and upper calypter of the wings.
Aktivitas Enzim Monooksigenase pada Larva Culex Quinquefasciatus di Kabupaten Brebes Nihayah, Husnatun; Mulyaningsih, Budi; Umniyati, Sitti Rahmah
BERKALA SAINSTEK Vol 8 No 3 (2020)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v8i3.17656

Abstract

Nyamuk Culex quinquefasciatus merupakan salah satu vektor penular penyakit filariasis bancrofti. Kabupaten Brebes adalah salah satu daerah endemik filariasis di Jawa Tengah. Upaya untuk mengurangi angka kejadian filariasis adalah dengan mengendalikan vektor penularnya. Salah satu jenis insektisida yang sering digunakan di kalangan masyarakat adalah dari golongan permetrin. Penggunaan insektisida dalam waktu lama dapat memicu timbulnya resistensi pada serangga. Resistensi dari golongan sintetik piretroid dapat diketahui melalui aktivitas enzim monooksigenase. Tujuan dari penelitian ini adalah untuk mengetahui aktivitas enzim monooksigenase pada populasi nyamuk Culex quinquefasciatus di Kabupaten Brebes. Nyamuk sampel berasal dari pemasangan ovitrap di daerah endemik dan nonendemik filariasis bancrofti di Kabupaten Brebes. Nyamuk dikembangbiakkan di Laboratorium Parasitologi Universitas Gadjah Mada sampai generasi ke dua. Uji aktivitas enzim monooksigenase dilakukan dengan metode yang dilakukan oleh Lee. Hasil penelitian menunjukkan adanya peningkatan aktivitas enzim monooksigenase pada daerah nonendemik sehingga statusnya menjadi toleran. Toleran terhadap insektisida sintetik piretroid pada populasi Culex quinquefasciatus di daerah nonendemik filariasis di Kabuaten Brebes disebabkan karena penggunaan insektisida piretroid yang juga digunakan sebagai insektisida pertanian di wilayah penelitian.
Struktur Histologi Trabekula Femur Mencit (Mus musculus L) Orkidektomi Pasca Pemberian Ekstrak tempe Kedelai Fajariyah, Susantin; Muta’alimah, Siti Nafi’atul; Utami, Eva Tyas; Lelono, Asmoro; Nihayah, Husnatun
JURNAL BIOS LOGOS Vol. 14 No. 3 (2024): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v14i3.56668

Abstract

Testosterone deficiency is caused by, among other things, old age, chronic disease, and testicular cancer, which results in decreased bone density. Estrogen plays a more important role in the process of bone growth and resorption than the hormone testosterone. Therefore, to prevent a decrease in density in conditions of testosterone deficiency, it is necessary to administer external phytoestrogens, including tempeh, containing isoflavones deidzein dan genistein. The aim of this study was to determine the effect of administering tempeh extract on the histological structure of the femoral trabeculae of orchidectomized mice. Mice were divided into 4 groups, namely negative control (without orchidectomy), positive control (orchidectomy), and 2 orchidectomy treatment groups, followed by administration of tempe extract at a dose of 0.6 g/ml/day (D1) and 1.2 g/ml/day (D2). Tempeh extract was administered by gavage for 15 days after a 30-day healing period for bilateral orchidectomy. One day after the last administration of tempeh extract, the mice were anesthetized and dissected to remove the femur bones to make preparations using the paraffin method and HE staining. The parameters observed were the number of osteoblasts and osteoclasts and the thickness of the femoral trabeculae. Giving soybean tempeh extract at a dose of 0.6 mg/ml/day by gavage for 15 days can cause an increase in the number of osteoblasts and trabecular thickness, as well as reducing the number of osteoclasts in the femur of orchidectomized mice.
Blood Glucose Level of Hyperglycemia Rats (Rattus novergicus) after Neem Gum (Azadirachta indica) Administration Utami, Eva Tyas; Fajariyah, Susantin; Lelono, Asmoro; Nihayah, Husnatun
Jurnal Biologi Tropis Vol. 25 No. 2 (2025): April-Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i2.8748

Abstract

Neem, a member of the Meliaceae family, is recognized to have the ability to decrease blood glucose levels. The study’s goal is to determine the neem gum (NG) impact in overcoming hyperglycemia in Wistar rats. There were three groups of rats in this study: (1) control, (2) hyperglycemic group (which got Streptozotocin (STZ) treatment but not NG treatment), and (3) hyperglycemic group (which got STZ treatment and 15% NG treatment) for three weeks. The T test results revealed notable variations before and after giving STZ. The finding indicated the blood glucose level before STZ induction in control was 86.50 ± 3.15 mg/dL, and the diabetic rat group (treated with STZ) was 87.60 ± 4.56 mg/dL. After STZ induction (7th day), there was a noteworthy rise in amount of glucose, namely 435.34 ± 120.33 mg/dL compare to the STZ treatment. The Duncan Multiple Range (DMRT) test showed there is no discernible difference between the STZ and NG treatments; however, there was a downward trend on days 14, 21, and 28. The conclusion of this study is that 15% NG has failed to decreas ehyperglycemia to normal blood glucose levels.
Counseling on The Importance of Maintaining Women's Reproductive Health In Arjasa Village, Arjasa Sub-District, Jember District Fajariyah, Susantin; Utami, Eva Tyas; Nihayah, Husnatun
Jurnal Inovasi Sains dan Teknologi untuk Masyarakat Vol. 3 No. 1 (2025): Mei
Publisher : Faculty of Mathematics and Natural Sciences, University of Jember. Jl. Kalimantan No.37, Krajan Timur, Jemberlor, Kec. Sumbersari, Jember Regency, East Java 68121

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/instem.v3i1.5487

Abstract

Women's reproductive health is an important aspect in maintaining community welfare, especially in preventing various diseases related to the reproductive organs. This counseling aims to increase the understanding and awareness of the community, especially mothers in Arjasa Village, about the importance of maintaining reproductive health. The method used in this activity is in the form of interactive counseling and discussion. The stages of service activities carried out: situation analysis, coordination with the village government, and implementation of activities. This activity involved 35 female participants aged 18-35 years, the majority of whom were housewives. The counseling material includes the definition of reproductive health, factors that affect it, common diseases, as well as prevention and early detection measures. The results of the activity showed that the participants had high enthusiasm in participating in the counseling, which could be seen from the active interaction during the question and answer session. The results of the activity showed a fairly good understanding of the counseling participants as seen from the enthusiasm of the participants in participating in the counseling and discussion sessions. The main obstacle in this activity was the limited time which caused some materials to be delivered briefly. Overall, this counseling has a positive impact in increasing awareness of women's reproductive health. It is expected that follow-up in the form of collaboration with village health cadres can continue to be carried out to maintain the reproductive health of the local community.
Moringa oleifera gum effect on blood sugar levels and rat pancreas histology induced by Streptozotocin Utami, Eva Tyas; Fajariyah, Susantin; Lelono, Asmoro; Nihayah, Husnatun
JPBIO (Jurnal Pendidikan Biologi) Vol 9, No 1 (2024): April 2024
Publisher : STKIP Persada Khatulistiwa Sintang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31932/jpbio.v9i1.3184

Abstract

Moringa gum is a product that people can use as an anti-hyperglycemic agent. This study aimed to determine the effectiveness of Moringa gum in reducing blood sugar levels and improving the histology structure of the rat pancreas induced by streptozotocin (STZ). This study used 21 male rats divided into 3 groups including control, diabetes (STZ), and diabetes (STZ + 3% Moringa gum). STZ treatment at a single dose (45 mg/kg BW) was given intraperitoneally, while Moringa gum was given through drinking water for 2 weeks. Blood sugar levels were measured on days 0, 7th, 14th, 21st, and 28th. The results showed that  STZ administration induced diabetes in rats with blood sugar levels of 487 mg/dL on the 14th day.  Moringa gum administration was able to reduce blood sugar levels on days 21st and 28th respectively to 306 and 234 mg/dl. The histology structure of the pancreas of rats treated with gum also showed improvement. This study concludes that Moringa gum is effective in lowering blood sugar levels in rats even though it still does not show normal blood sugar levels, and can repair STZ-induced damage to the histology structure of the rat pancreas.
Mosquitoes' larval habitat characteristics and Aedes aegypti resistance status to malathion in Jember Nihayah, Husnatun; Purwatiningsih, Purwatiningsih; Utami, Eva Tyas; Fajariyah, Susantin; Lelono, Asmoro; Puspitasari, Reni; Rohmah, Hidayatur
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 10 No. 02 (2025): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v10i02.1037

Abstract

Water-holding containers serve as breeding sites for Aedes aegypti. The control of mosquito breeding sites and the use of household insecticides are some of the DHF control methods. The use of household insecticides over a long period can reduce the susceptibility of mosquitoes to these insecticides. This research aims to determine the mosquito's habitat characteristics and to determine the Aedes aegypti resistance status to malathion in Jember.  A total of 61 houses were surveyed, and the various types of containers encountered at the sampling locations were recorded. Larvae and pupae found at the sampling location are brought and hatched in the laboratory. Aedes aegypti was tested for resistance status to malathion insecticide using a CDC bottle bioassay and biochemical nonspecific esterase enzyme activity. A total of 140 containers were found in this study, and 36.43% of them are Aedes aegypti larvae/pupae habitat. Types of containers used as mosquito larval habitat are bottles/glasses, bathtubs, used basins/buckets, water storage buckets, trash cans, refrigerator water containers, used cans, gutters, livestock drinking water containers, fish ponds, plant pots, used livestock cages, used toilets, used jugs, used animal feeders, and used aquariums. The mortality of Aedes aegypti reached 42.3% (resistant). Meanwhile, the results of biochemical tests showed an increase in the activity of the nonspecific esterase enzyme. This study concludes that Aedes aegypti from Jember has been resistant to malathion insecticide
Co-Authors Budi Mulyaningsih Eva Tyas Utami Lelono, Asmoro Muta’alimah, Siti Nafi’atul Purwatiningsih Purwatiningsih Puspitasari, Reni Rohmah, Hidayatur Sitti Rahmah Umniyati Susantin Fajariyah