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All Journal Jurnal Sains dan Teknologi Meditory : The Journal of Medical Laboratory Jurnal Pengabmas Masyarakat Sehat Jurnal Kesehatan Lingkungan (JKL) Jurnal Pengabmas Masyarakat Sehat JURNAL SKALA HUSADA: THE JOURNAL OF HEALTH
Suyasa, I Nyoman Gede
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Biochemistry, Genetics & Molecular Biology Computer Science & IT Dentistry Education Environmental Science Health Professions Nursing Public Health Veterinary

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Journal : JURNAL SKALA HUSADA: THE JOURNAL OF HEALTH

 1 
UJI AKTIVITAS ANTIBAKTERI Staphylococcus aureus BERDASARKAN PERBEDAAN JENIS PELARUT EKSTRAK BIJI BUAH PEPAYA (Carica papaya L.) Lisa Anjani, Ni Putu; Suyasa, I Nyoman Gede; Habibah, Nur; Purna, I Nyoman
Jurnal Skala Husada : The Journal of Health Vol 22, No 1 (2025): Jurnal Skala Husada (JSH): The Journal of Health
Publisher : Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/jsh:tjoh.v22i1.3946

Abstract

Staphylococcus aureus is an opportunistic pathogenic bacteria on the surface of the skin that causes impetigo. Treatment therapy for impetigo can be done by administering natural medication from papaya seeds (Carica papaya L). This study aims to determine the antibacterial activity of Staphylococcus aureus papaya seed extract based on the different types of extract solvents used. This research is true experimental research with a posttest only control design research form. The research method used was the disk diffusion method with three treatments of papaya seed extract, namely 96% ethanol extract, ethyl acetate and n-hexane, positive control using chloramphenicol and negative control using 96% ethanol, ethyl acetate and n-hexane. The research results showed that each papaya seed extract treatment was able to inhibit Staphylococcus aureus with an inhibition zone of 96% ethanol extract of 18.09 mm, ethyl acetate extract of 7.93 mm, n-hexane extract of 0.36 mm. The One Way Anova test showed a value of ρα(0.05) so that there was a significant difference in the diameter of the inhibition zone in each extract treatment. The Tukey test showed that there were significant differences in the inhibition zone of Staphylococcus aureus bacteria (ρα(0.05)) in almost all treatments. The conclusion of this research is that there is a difference in the antibacterial activity of 96% ethanol extract, ethyl acetate extract and n-hexane extract of papaya seeds on the growth of Sthaphylococcus aureus bacteria.
PENGARUH PENGGUNAAN EKSTRAK BUNGA PACAR AIR (Impatiens balsamina L.) SEBAGAI ANTISEPTIK TERHADAP ANGKA KUMAN PADA TELAPAK TANGAN MAHASISWA POLTEKKES DENPASAR Pradnyani Putri, Dewa Ayu; Suyasa, I Nyoman Gede; Widhya HS, Cok Dewi; Suyasa, Ida Bagus Oka
Jurnal Skala Husada : The Journal of Health Vol 21, No 1 (2024): Jurnal Skala Husada (JSH)-The Journal of Health
Publisher : Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/jsh:tjoh.v21i1.3314

Abstract

Pacar air flowers (Impatiens balsamina L.) hold potential as natural antibacterial agents, rich in compounds like flavonoids, alkaloids, steroidal saponins, tannins, quinones, and phenols. Pacar air flowers are utilized in traditional medicine for various medical conditions, including antibacterial properties. The purpose of this study is to determine the effect of using balsam flower extract (Impatiens balsamina L.) as a natural antiseptic in reducing total plate count on the hands of Poltekkes Denpasar students. Sterile hand swab samples were collected using a Pretest-Posttest Control Group Design to assess differences before and after the application of various concentrations. Statistical analysis was performed using One-way ANOVA. The study revealed differences in total plate count before and after the application of pacar air flower extract (Impatiens balsamina L.) on the hands of Poltekkes Denpasar students. The mean total plate count before treatment was 29,98 CFU/cm2, while after treatment, it was 4,70 CFU/cm2. Total plate count significantly decreased (p0.05) after applying the pacar air extract compared to before (Impatiens balsamina L.). There was a significant reduction in total plate count with the application of extract concentrations of 5%, 10%, and 15%
Co-Authors I Nyoman Jirna, I Nyoman I Nyoman Purna Jirna, I Nyoman Nyoman Gede Lisa Anjani, Ni Putu Mayumi, Ni Kadek Sintya Nur Habibah Oka Suyasa, Ida Bagus Posmaningsih, D.A.A Pradnyani Putri, Dewa Ayu Rusminingsih, Ni Ketut Sudarmanto, I Gede Suyasa, Ida Bagus Oka Widhya HS, Cok Dewi Yulia, Ni Putu Mentari