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The potential of traditional balinese spices against the growth of Salmonella sp in vitro Jirna, I Nyoman; Sudarmanto, I Gede; Kurniawan, Surya Bayu; Ratih, Gusti Ayu Made; Rasyid, Burhannuddin
Jurnal Teknologi Laboratorium Vol 9 No 1 (2020): 2020 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29238/teknolabjournal.v9i1.200

Prevention by utilizing medicinal plants can be used as an alternative treatment. This study aims to identify active antimicrobial substances and test the antimicrobial potential of traditional Balinese spices, namely Basa Selem, Basa Gede, Basa Wangen, Basa Bawang Jahe, and Basa Rajang against Salmonella sp bacteria in vitro. The True-experimental method with Posttest only-control design was used in this study, by intervening in the treatment group as well as the presence of positive and negative controls. The maceration method with 96% EtOH solvent was used to extract active substances and identify the levels of antimicrobial active substances. The TLC Spectrophotodensitometer instrument and the diffusion method (discs) were used to test antimicrobial potential. Data analysis was performed using the one-way ANOVA test. All five samples showed flavonoids, alkaloids, tannins, and phenols in qualitative tests. Based on quantitative test results of five samples, the highest compound content obtained in Basa Wangen (6.66 mg/ml of tannins), Basa Gede (3.74 mg/ml of flavonoids), Basa Bawang Jahe (2.49 mg/ml of tannins), Basa Selem (2.87 mg/ml of tannin), and Basa Rajang (6.96 mg/ml of flavonoids). There are differences in the antimicrobial potential of various types of traditional Balinese spices against the growth of Salmonella sp in vitro (sig = 0.037). The traditional Balinese spices have the antimicrobial potential of the intermediate category with a range of inhibition (16-20 mm) based on the NCCLS standard.

ISOLASI DAN UJI AKTIVITAS ANTIMIKROBA BAKTERI ASAM LAKTAT DARI BLONDO VIRGIN COCONUT OIL TERHADAP BAKTERI Staphylococcus aureus Rasyid, Burhannuddin; Sandi, Ketut Meina; Sudarmanto, I Gede; Karta, I Wayan
Biomedika Vol 13, No 1 (2021): Biomedika Februari 2021
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v13i1.11070

ABSTRAKBlondo merupakan limbah produksi virgin coconut oil (VCO) dapat menjadi sumber untuk mengisolasi bakteri asam laktat. Bakteri asam laktat mampu menghasilkan zat antimikroba yang dapat dikembangkan untuk mengobati kasus infeksi oleh bakteri Staphylococcus aureus. Bakteri Staphylococcus aureus dapat menyebabkan infeksi mulai dari keracunan makanan, infeksi kulit hingga infeksi sistemik. Penelitian ini bertujuan untuk mengisolasi dan menguji aktivitas antimikroba bakteri asam laktat dari blondo terhadap bakteri Staphylococcus aureus.Â Penelitian ini merupakan penelitian deskriptif cross-sectional. Sampel blondo diambil dari kelompok wanita tani di Tabanan yang berjumlah 16 sampel dengan pengambilan sampel secara simple random sampling.Â Bakteri diisolasi menggunakan media selektif yaitu agar de Man Rogosa and Sharpe (MRS) dan identifikasi dengan uji pewarnaan gram, uji katalase dan uji produksi gas dari glukosa. Uji aktivitas antimikroba bakteri asam laktat dilakukan dengan menggunakan metode difusi sumuran. Hasil penelitian ini menunjukkan bahwa di dalam sampel blondo terdapat bakteri asam laktat dari genus Lactobacillus dan Streptococcus. Bakteri asam laktat yang diisolasi dari blondo VCO mampu menghambat Staphylococcus aureus dengan terbentuknya zona hambat berupa zona bening dengan rata-rata diameter zona hambat 11,02 mm. Zona hambat yang terbentuk termasuk dalam kategori sedang dan kuat, dengan zona hambat tertinggi yang dihasilkan yaitu 20,8 mm dan terendah yaitu 9,2 mm.Â Bakteri asam laktat dari blondo memiliki aktivitas antimikroba terhadap bakteri Staphylococcus aureus.Kata Kunci: Bakteri Asam Laktat, Blondo, Potensi AntimikrobaÂ ABSTRACTBlondo is a waste from the virgin coconut oil (VCO) producted which can be used as a source of lactic acid bacteria. Lactic acid bacteria are capable of producing antimicrobial compounds to inhibit the growth of pathogenic bacteria. The purpose of this study was to isolate and test the antimicrobial activity of lactic acid bacteria from blondo against Staphylococcus aureus bacteria. This research is a descriptive crossÂ¬-sectional. Blondo samples were taken at group of farm women in Tabanan Regency, which was 16 samples with simple random sampling. Bacteria were isolated using selective medium de Man Rogosa and Sharpe (MRS) agar and identified with gram staining test, catalase test and gas production test from glucose. The antimicrobial activity test of lactic acid bacteria Â was performed by using wells-diffusion method. The results of this study indicated there was lactic acid bacteria from the genus Lactobacillus and Streptococcus in the blondo sample. Lactic acid bacteria isolated from blondo were able to inhibit Staphylococcus aureus by forming a clear zone with an average diameter of inhibition zone was 11.02 mm. There were two antimicrobial potential category, medium and strong category, with the highest resulting inhibit zone was 20.8 mm and the lowest was 9.2 mm. Lactic acid bacteria from blondo had antimicrobials potential against Staphylococcus aureusÂ Keywords: Lactic Acid Bacteria, Blondo, Antimicrobial Potential

PEMBERDAYAAN PENGELOLA PASAR DESA PAKRAMAN PANJER DALAM DETEKSI DINI PENYAKIT TIDAK MENULAR Jirna, I Nyoman; Sudarmanto, I Gede; Suyasa, I Nyoman Gede
Jurnal Pengabmas Masyarakat Sehat Vol 7, No 1 (2025): JANUARI
Publisher : Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/jpms.v7i1.3787

Pakraman Panjer Village Market is the economic center of the Panjer traditional village located on Jalan Pakerisan Denpasar Bali. The results of the initial survey of 10 traders showed that the problem was that 50% of traders had low activity levels, 10% had a heavy physical appearance and tended to be obese, 50% were in the elderly category, 30% consumed foods high in cholesterol and carbohydrates/sugar, 10% were active smokers, 60% had a level of knowledge. low, 10% routinely carry out early examinations. This data shows that the targeted 100% health services/screening and early detection of NCD risk factors have not been achieved.The service method is in the form of counseling, training, empowerment, checking PTM (cholesterol, glucose, uric acid, blood pressure) for traders and market managers in Pakraman Panjer village. Partner's contribution as a role model through cadres formed for the sustainability of this Community Service activity.There was an increase in knowledge from 50 targets before and after counseling, namely 30 people (60%). an increase in normal cholesterol in 8 people (16%) s, an increase in normal glucose in 8 people (16%), an increase in uric acid in 8 people (16%) and an increase in normal blood pressure in 13 people (26%) before and after coaching. As a result of coaching 10 market managers, there was an increase in their ability as instructors, namely 6 people (60%) to 9 people (90%).Extension workers formed from market managers carry out continuous guidance to traders and related agencies (puskesmas) provide assistance and supervision.Keywords:. Empowerment, Early Detection, Non-Communicable Diseases,

PEMBERDAYAAN PENGELOLA PASAR DESA PAKRAMAN PANJER DALAM DETEKSI DINI PENYAKIT TIDAK MENULAR Jirna, I Nyoman; Sudarmanto, I Gede; Suyasa, I Nyoman Gede
Jurnal Pengabmas Masyarakat Sehat Vol 7, No 1 (2025): JANUARI
Publisher : Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/jpms.v7i1.3787

Pakraman Panjer Village Market is the economic center of the Panjer traditional village located on Jalan Pakerisan Denpasar Bali. The results of the initial survey of 10 traders showed that the problem was that 50% of traders had low activity levels, 10% had a heavy physical appearance and tended to be obese, 50% were in the elderly category, 30% consumed foods high in cholesterol and carbohydrates/sugar, 10% were active smokers, 60% had a level of knowledge. low, 10% routinely carry out early examinations. This data shows that the targeted 100% health services/screening and early detection of NCD risk factors have not been achieved.The service method is in the form of counseling, training, empowerment, checking PTM (cholesterol, glucose, uric acid, blood pressure) for traders and market managers in Pakraman Panjer village. Partner's contribution as a role model through cadres formed for the sustainability of this Community Service activity.There was an increase in knowledge from 50 targets before and after counseling, namely 30 people (60%). an increase in normal cholesterol in 8 people (16%) s, an increase in normal glucose in 8 people (16%), an increase in uric acid in 8 people (16%) and an increase in normal blood pressure in 13 people (26%) before and after coaching. As a result of coaching 10 market managers, there was an increase in their ability as instructors, namely 6 people (60%) to 9 people (90%).Extension workers formed from market managers carry out continuous guidance to traders and related agencies (puskesmas) provide assistance and supervision.Keywords:. Empowerment, Early Detection, Non-Communicable Diseases,

Improving Work Attitudes And Assistive Devices Can Reduce Subjective Complaints And Increase The Productivity Of Weavers Sudarmanto, I Gede; Purna, I Nyoman; Jirna, I Nyoman; Mastra, I Nyoman; Rusminingsih, Ni Ketut; Sudiadnyana, I Wayan
International Journal of Islamic Education, Research and Multiculturalism (IJIERM) Vol 5 No 1 (2023)
Publisher : The Islamic Education and Multiculturalism Foundation

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47006/ijierm.v5i1.205

Gringsing weaving products is a part of handicraft or home industry. They are still confessed at present and produced by simple technology with hand in hand. This activity as a business of income from the most of women in Tenganan Pegringsingan Village of Manggis Sub District, Karangasem Regency. A work attitude with no planned well and they do not use a tool instruments for helping then working correctly, it is caused a subjective complaints of the workers so the production is not maximally and will cause the productivity of the workers come down. Woven products workers is required a carefulness and neatness of the workers, with long sitting posture on the flour covered by something material. It is not aware of this problem in to the anatomy and physiology of the workers and they will have a lot of complaint, sick, or pain on their next, hand, low back, anus, thigh, eyes. It is caused static work load frequently, so that they will be to come a decrease activity and low motivations, it’is activity and the work is not done efficient and effective. The variable that is measured in this study is decrease workload, subjective complaint, work efficiency (long work), and work productivity. The research has been studied to the sixteen weaving workers that were chosen without rules with treatment by subject program with cross over design, its analyses unit considering group variance. It has been intervened by work attitude with supporting tool for instant ‘peliper, tingklik, por (supported by sponge), barble, and using eyes glasses and group workers. This study is analyzed by t-paired, and there is a significant work load reduction (p<0.005), which is the pulse of the workers come down from 89,62 beats per minutes to be 82,56 beats per minutes (±5,60) in the same of the light work load category. The subjective complaints of the work load come down showed significant value (p<0.005) that is from Nordic Body Map score 56,92 (16.14) to be 28.86 (7.32). By the efficient work 7 hours a day with support tools, it is to be come the productivity increase (0,34%) and work productivity (7,9%) significantly (p<0.005).

Differences in Bleed Sedimentation Rate and Hematocrit Values in Chronic Kidney Failure Patients Examined Before and After Hemodialysis at Tabanan Regional Hospital Cahyana, I Made Dwipa; Bekti, Heri Setiyo; Sudarmanto, I Gede
MEDICA (International Medical Scientific Journal) Vol. 6 No. 2 (2024): MEDICA (Internasional Medical Scientific Journal)
Publisher : Borneo Scientific Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53770/medica.v6i2.501

Chronic Kidney Disease (CKD) is a pathological condition that is irreversible and has various underlying causes. The diagnosis is based on structural or functional abnormalities of the kidneys, with the primary criterion being a glomerular filtration rate (GFR) of less than 60 mL/min/1.73 m² for more than three months. Common symptoms include fatigue, loss of appetite, and muscle cramps. Management of CKD consists of two stages: conservative treatment aimed at slowing disease progression and renal replacement therapy, such as dialysis or kidney transplantation. Erythrocyte Sedimentation Rate (ESR) and Hematocrit (Hct) tests are important for detecting decreased erythropoietin production, which impacts red blood cell formation. The objective of this study was to examine the differences in ESR and Hct values in patients with chronic kidney disease before and after undergoing hemodialysis. The study employed a pre-experimental design using a One-Group Pretest-Posttest approach, involving a single experimental group without a control group. Measurements were taken both before and after the intervention. The results from 10 CKD patients at Tabanan Regional Public Hospital indicated that all respondents (100%) had elevated ESR levels both before and after hemodialysis. In contrast, Hct values decreased following hemodialysis, which is likely due to reduced erythropoietin production associated with CKD, leading to anemia. In conclusion, no significant difference was observed in ESR values before and after hemodialysis. However, there was a notable difference in Hct values, which declined after the procedure.

DESCRIPTION OF URIC ACID LEVELS BUS DRIVER PT ARSA BUANA MANUNGGAL KARANGASEM Yulia, Ni Putu Mentari; oka suyasa, ida bagus; sudarmanto, i gede; suyasa, i nyoman gede
Meditory : The Journal of Medical Laboratory Vol 12, No 1 (2024): Meditory, Volume 12 No. 1 Tahun 2024
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v12i1.3209

Uric acid is the end result of breaking down a substance called purine. Purine substance is a natural substance which one of the chemical structure groups is the formation of DNA and RNA. Uric acid can be influenced by several factors such as age, long sitting while driving, consumption of alcoholic beverages, and consumption of foods high in purines. The aim of this study was to determine uric acid levels in PT.ABM drivers. The study sample consisted of 45 people selected by random sampling using the POCT method with the Autoclick Easy Touch GCU tool. The results showed normal uric acid levels in 32 people (71.1%) and high uric acid levels in 13 people (28.9%). High uric acid levels were more common at the age of 43-51 years in 5 people (45.5%), duration of sitting while driving was dominated by driving duration for ? 7 hours/day in 10 people (29.4%), while drivers 11 people (34.4%) consumed alcoholic beverages and 25 people (55.6%) consumed high-purine foods. The conclusion of this study is that high uric acid levels occur in respondents aged 43-51 years, driving for ? 7 hours/day, consuming alcoholic beverages and consuming foods high in purines.

Kadar Debu Terhirup Menurunkan Kapasitas Vital Paru-Paru Tenaga Kerja Iron Candle Sudarmanto, I Gede; Purna, I Nyoman; Suyasa, I Nyoman Gede; Jirna, I Nyoman; Posmaningsih, D.A.A
Jurnal Kesehatan Lingkungan (JKL) Vol 12, No 2 (2022)
Publisher : Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/jkl.v12i2.2220

Dust particles are a collection of compounds in the form of solid or liquid which are scattered in the air with a very small diameter, less than 1 micron to a maximum of 500 microns. The size of dust particles that are harmful to health generally ranges from 0.1 microns to 10 microns. The dust particles will be in the air for a relatively long time in a hovering state. The work of crafting candle holders (iron candles), in the production process, allows for accidents and occupational diseases to arise from the stages of the manufacturing process. At the stage of the production process in the sanding room, a lot of dust is produced. There are workers in this section when working, some use masks and some do not. Even those who use masks are carried out infrequently, if by chance there is an inspection from the Regional Government or from the local Ministry of Manpower. The work environment is classified as hazardous to the health of the workers because dust particles can enter the body through the respiratory tract. A case control study has been conducted on 48 male and female workers at four iron candle craft companies in Tabanan district for 6 months, which is divided into 24 case groups and 24 control groups taken by cluster random sampling. The variables measured were the level of inhaled dust and the vital capacity of the workers' lungs. Analysis with the t-Paired test showed that there was a high causal relationship (significance) between the level of dust inhaled in the sanding work environment of the iron candle craft business in Tabanan district and the decrease in vital lung capacity of male and female workers.Keywords: Dust Content, Lung Vital Capacity.

Efektifitas Pendidikan Kesehatan Dengan Media Booklet Terhadap Peningkatan Partisipasi Masyarakat Pada Bank Sampah Di Kabupaten Badung Suyasa, I Nyoman Gede; Sudarmanto, I Gede; Purna, I Nyoman
Jurnal Kesehatan Lingkungan (JKL) Vol 13, No 2 (2023)
Publisher : Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/jkl.v13i2.2315

Waste dumps are affected by overcrowding and human activity. Badung Regency produces 250 tons of garbage per day, to handle the waste problem Badung District Government is one of its efforts to establish a waste bank. This garbage bank was formed in the neighborhood of banjar, villages, schools and private institutions. The purpose of the study was to find out the increase in community participation in the waste bank program before and after health education with the media booklet. This type of research is pre-experimental research with one group pre-test-post-test design. The study population is the head of the family in Badung Regency. The sample size calculation obtained a sample of 337 people. Sampling with cluster random sampling, data collected using a questionnaire. Data collection before treatment and one month after treatment. Data analysis with paired t-test statistics. The results obtained by the level of community participation in waste banks before health education with the media booklet is very less 106 people (31.45%), less 14 people (4.15%), 40 people enough (11.87%), both 105 people ( 31.16%) and very good 72 people (21.36%). The level of community participation in the waste bank after health education with the media booklet is 31 people (9.20%), 175 people (51.93%) and very good 131 people (38.87%). And there are differences in community participation in waste banks before and after health education with a media booklet with a significance of 0,000. It is expected that the management of the official waste bank related to socialize the waste bank program to the public with the media booklet, facilitate prices to be stable and prepare transportation and personnel to take garbage to the homes of residents.

KENYAMANAN BALE METEN RUMAH TRADISIONAL DITINJAU DARI SUDUT ERGONOMIS DI DESA ADAT BATUAN SUKAWATI GIANYAR BALI Sudarmanto, I Gede; Mahayana, I Made Bulda; Jirna, I Nyoman
Jurnal Kesehatan Lingkungan (JKL) Vol 14, No 1 (2024)
Publisher : Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/jkl.v14i1.3204

Abstract. Residential houses have forms and functions to protect humans from natural influences. A good house must meet several requirements such as; environmental planning, spatial planning, building planning and utilities. Traditional Balinese architecture basically combines two main human needs, namely "scale-niskala" (both inner and outer), which is based on Hindu religious philosophy starting from spatial planning, forms, materials and structures. Problems with the original "Bale Meten" such as: dust, stuffiness, smell, humidity 59-63%, lighting 10-60 lux. Due to the times, "Bale Meten" automatically changes to a new function, namely for studying and sleeping, so that many people make modifications to their "Bale Meten". This research was conducted in the Batuan Traditional Village, Sukawati District, Gianyar Regency, Bali, in 2022, with the aim of finding out whether the original "Bale Meten" and modified "Bale Meten" meet ergonomic requirements or not. The sample consists of 60 "Bale Meten", with variables including: lighting, temperature, humidity, air circulation (micro climate). This type of research is "descriptive exploratory" with an "analytical cross-sectional" design. Data collection was carried out by way of interviews, observation, and measurement, while data analysis was in the form of "descriptive and analytic". The results obtained stated that the comfort of the original "Bale Meten" and the modified "Bale Meten" did not meet the ergonomic requirements. Microclimate affects comfort, while windows and ventilation affect microclimate. The solution is to socialize to the community how to build houses that meet technical and ergonomic requirements, so that they become healthy and livable homes.

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