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The Effects of Propolis Ethanol Extract on The Decreasing Number of The Mouses Spermatids Induced by Cigarettes Smoke Mugi Tri Sutikno; . Muthmainah; Riza Novierta Pesik
Nexus Biomedika Vol 4, No 1 (2015): Nexus Biomedika
Publisher : Nexus Biomedika

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Background:The propolis ethanol extract contains flavonoid, terpenoid, Caffeic Acid Phenethyl Ester (CAPE), vitamin A, vitamin C and vitamin E which are potential for being antioxidant to protect the spermatids from the danger of free radical. The aims of this research were to know the effects of propolis ethanol extract on the decreasing number of the mouses spermatids induced by cigarettes smoke and to know the effect of dosage raising level of the propolis ethanol extract towards the research subject. Methods:This research was a laboratory experiment using the posttest only controlled group design. The sampels were 25 Swiss Webster mice (age around 2-3 months and approximately 20 grams of weight). They were classified into 5 groups which consisted of 5 mice in each group. KK group was without any intervention. KI group was only induced by the cigarettes smoke. KP1, KP2 and KP3, all were induced by the cigarettes smoke and in a row, got 5.6 mg (dosage level I), 11.2 mg (dosage level II) and 16.8 mg (dosage level III) of propolis ethanol extract. These interventions were done for two weeks. The decreasing number of spermatids was counted through the inspection under microscope and counting of five chosen rounded-looks seminiferous tubules. The data were analyzed by One-Way ANOVA test and Post Hoc Multiple Comparisons test. Results:The result of One-way ANOVA p = 0.000 (p < ?) showed differences between each research groups. The Post Hoc test of the spermatids number showed significant differences between KK-KI, KK-KP1, KK-KP2, KK-KP3, KI-KP1, KI-KP2, KP1-KP2, KP1-KP3, KP2 -KP3 groups but KI and KP3 wasnt. Conclusions:The propolis ethanolic extract can inhibit the decreasing number of the mouses spermatids which is induced by cigarettes smoke and the raising dosage from 5.6 mg to 11.2 mg is the succeeded dosage to inhibit the decreasing number of the spermatids. Keywords: propolis ethanol extract, spermatid, mouse, cigarettes smoke.

Pengaruh Ekstrak Air Buah Nanas terhadap Ekspresi Caspase-3 dan Fragmentasi DNA Sel Adenokarsinoma Kolorektal WiDr Sintin Khotijah Pribadi; Dyah Ratna Budiani; Riza Novierta Pesik
Nexus Biomedika Vol 3, No 1 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: Colorectal adenocarcinoma is the third most diagnosed neoplasm in the world. Many approaches have been taken for therapy, especially targeted therapy. However, the resistance event still occurs due to constitutive activation of intracellular signaling. In the other hand, pineapple fruit [Ananas comosus (L.) Merr.] has bromelain enzyme that can specifically influence intracellular signaling and not only lead to inhibit proliferation but also lead apoptosis induction in neoplasm cell. The purpose of this study was to elucidate the effect of pineapple fruit aqueous extract to caspase-3 expression and DNA fragmentation of apoptosis process in WiDr colorectal adenocarcinoma cells. Methods: This research was an experimental laboratory study with the post test only control group design. The samples of this research were WiDr colorectal adenocarcinoma cells divided into 6 groups: Negative control group, Positive control group with doxorubicin 5 ?g/ml and four Treatment groups with 270 ?g/ml, 202.5 ?g/ml, 135 ?g/ml and 67.5 ?g/ml of extract concentration, each. The samples were incubated for 48 hours in 37oC and 5% CO2. The caspase-3 expression was determined by cytologic score of samples stained with caspase-3 antihuman antibody. The DNA fragmentation was determined by 1% agarose gel electrophoresis. The cytologic score was analyzed by one way ANOVA test and LSD test. Result: The result showed that there was significant difference between Positive control group and the other groups (p=0.000), but there wasnt significant difference between the Negative control group and the Treatment groups (p>0.05). The result from electrophoresis showed that there wasnt DNA fragmentation in WiDr colorectal adenocarcinoma cells. Conclusion: The pineapple fruit aqueous extract did not increase caspase-3 expression and did not lead DNA fragmentation to WiDr colorectal adenocarcinoma cells for 48 hours of treatment. Keywords: pineapple fruit aqueous extract, WiDr colorectal adenocarcinoma cell, caspase-3 expression, DNA fragmentation

Molecular Docking Analysis of Family Fabaceae Phytochemistry to Estrogen Receptor Alpha Compared to Tamoxifen in Breast Cancer Ega Caesaria Pratama Putra; Riza Novierta Pesik; Ratna Kusumawati
Nexus Kedokteran Translasional Vol 4, No 1 (2015): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Background: Breast cancer is the second most common cancer.Estrogen is the risk factor of breast cancer. Estrogen receptor ? is expressed by 50-80% of breast cancer. The mechanism of estrogen is mediated by estrogen receptor ? and estrogen receptor ?. Tamoxifen is a selective estrogen receptor modulators (SERMs) that can be bound to the estrogen receptor ?, therefore prevent bonding between estrogen and estrogen receptor ?. Methods: This research used molecular docking analysis of family Fabaceaes Phytochemistry as an inhibitor of estrogen receptor ? activation. This study was observational bioinformatics study to observe interaction between family Fabaceaes phytochemistry and estrogen receptor?. Molecular docking analysis observed binding energy and binding location. This virtual screening analysis was done using PyRx, AutoDock Vina, PyMOL, Open Babel, and UCSF Chimera. Results: We obtained 471 Family Fabaceaes phytochemistry from database, only five compounds that have equal or lower binding energy compared with tamoxifen, that compound werebeta-amyrine (9.6 KJ/Mol), obovatin( 9.6 KJ/Mol), erythrabyssin II (9.6 KJ/Mol), Cajaflavanone (10.2 KJ/Mol), and tomentosanol E (10.5 KJ/Mol). The visualization of binding location analysis showed that only cajaflavanone which have relatively similar binding site location with tamoxifen. Conclusions: Cajaflavanone have a similar characteristic with tamoxifen, and have a potency to be used as partial antagonist of estrogen receptor alpha in breast cancer based molecular docking analysis. However, In vitro and In vivo researchneeded to determine the effectiveness. Keywords: Breast Cancer, Molecular Docking, Fabacea, Tamoxifen, Estrogen Receptor ?

Molecular Docking Analysis of Estrogen Receptor ? to Phytochemistries in Asteraceae Family Compared to Tamoxifen in Breast Cancer Dzulfiar Nasir Umam; Riza Novierta Pesik; Ratna Kusumawati
Nexus Kedokteran Translasional Vol 4, No 2 (2015): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Background:Tamoxifen is the first line hormonal therapy for Estrogen Receptor ?(ER?) breast cancer. Some of phytochemistries pose anticancer activities. However, data of those from Asteraceae family in Indonesia are lacking. The aim of this research is to know the binding affinities and binding locations of phytochemistries in Asteraceae family from Indonesia compared to tamoxifen on ER? in breast cancer. Methods: This research wasa bioinformatic study. Subjects were3-dimensional structures ofER?, tamoxifen, and phytochemistriess Asteraceae family obtained from Indonesian HerbalDB database, Pubchem Compound, and Protein Data Bank. Subjects were prepared by Chimera 1.10 and Open Babel. Tamoxifen was docked on ER? by AutoDock Vina to know its binding affinities of ligand-receptor complex, compared to the other chosen ligands. Binding locations of ligand-reseptor were visualized by Pymol 1.7.2. Results: The docking results showed four phytochemistriess binding affinities whichwere stronger than tamoxifen (-9.6 kcal/mol), such as lappadilactone, friedelin, benperidol, and beta-amyrin. The visualization results showed that lappadilactone, friedelin, benperidol, beta-amyrin, taraxerol, andepifriedelanol had similar binding areas totamoxifen on ER?. Lappadilactone had hydrogen bond with Tyr526 and benperidol had hydrogen bond with Trp383 on ER?. Conclusions:Lappadilactone and benperidol have stronger binding affinities than tamoxifen and they have hydrogen bond with ER?, but not in active sites of ER?. Therefore, lappadilactone and benperidol may have ability to inhibit ER? activities computationally. Keywords: Estrogen receptor ?, molecular docking, phytocemistry, tamoxifen.

A Potential Candidate of Lactate Dehydrogenase Inhibitor Derived from Indonesia Herbal Compounds Adam Haviyan Nasrullah; Dono Indarto; Riza Novierta Pesik; R. AJ. Sri Wulandari
Nexus Kedokteran Translasional Vol 6, No 1 (2017): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Introduction: Lactate dehydrogenase A (LDHA) is an enzyme that catalyzes pyruvate into lactate. LDHA plays an important role in promotion of cancer cells growth through increasing aerobic glycolysis. Because LDHA has a central role in energy metabolism, it become a molecular target for development of anticancer drug. This was a biocomputational study that aimed to identify Indonesian herbal compounds which became a potential candidate of LDHA inhibitor via molecular docking analysis. Methods: Samples in this study were Indonesian herbal compounds that met the following criteria: (1) Registered on Database Herbal Indonesia, (2) had three-dimensional structure, and (3) met the criteria Lipinski rule of five. Oxamate used as a ligand standard and was validated using Autodock Vina software. Herbal compounds were also docked using the same program. Docking results were visualized using PyMOL software. LDHA inhibitor candidate is determined by comparing herbal compounds and standard ligand in terms of binding energy, binding site and Lipinski criteria. Result: Oxamate interacting with LDHA had -4.26 0.06 kcal / mol binding energy and bound to six amino acid residues at Gln 99, Arg 105, Asn 137, Arg 168, His 192, and Thr 247. A lower binding energy was observed in 23 herbal compounds and these compounds bound to LDHA at least five amino acid residues like Oxamate. Herbal compounds Phaseolic Acid, Sebacic Acid, D (-) - Fructose, Suberic Acid and Pimelic Acid interacted with amino acid residues of LDHA as same as Oxamate. The other herbal compounds interacted with less or more than six amino acid residues of LDHA. Based on characteristics of five herbal compounds, Phaseolic Acid, Sebacic Acid and Suberic Acid were probably the best candidates of LDHA inhibitor. Conclusion: Phaseolic Acid, Sebacic Acid and Suberic Acid become biocomputationally the best LDHA inhibitor. Enzymatic assays are needed to investigate whether or not all these compounds can inhibit LDHA enzyme activity. Keywords : Cancer, Inhibitor LDHA, Molecular Docking, Herbal Indonesia

Herbal Usage History as a Risk Factor for Delay in Treatment on Breast Cancer Patients in Surakarta Niluh Ayu Anissa Hanum; Kristanto Yuli Yarso; Riza Novierta Pesik
Nexus Kedokteran Klinik Vol 5, No 3 (2016): Nexus Kedokteran Klinik
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Introduction: Breast cancer is a most commonly diagnosed cancer in women with an estimated 1.67 million cases diagnosed in 2012, i.e. 25% of all cancer cases. As many as 69% of breast cancer incidence was found to have reached stage III and IV, which has lower five-years survival rate. Various factors play a role in the delay of treatment, one of them is an herbal usage history. This study aims to determine whether an herbal usage history is a risk factor of delay in breast cancer patients in Surakarta. Methods: This study design was observational analytic with cross sectional approach. The samples were 45 breast cancer patients with delayed treatment and 55 breast cancer patients without delay treatment in RSUD Dr. Moewardi and RSU Kasih Ibu Surakarta. Samples were taken by fixed disease sampling, which then selected based on inclusion and exclusion criteria. The instrument used is the patient records and supplementary questionnaire. The variables studied were delays in treatment with an herbal usage history along with other confounding factors that may affect the delay of treatment. Data then analyzed using bivariate test and logistic regression. Results: The results of the bivariate analysis showed that an herbal usage history is a risk factor of delay in treatment, with p = 0.002 and OR = 3.69. While the results of the multivariate analysis showed that a low level of knowledge affect the most to delays in treatment of breast cancer with OR = 3.699. Conclusions:Herbal usage history was proved as a risk factor for delay in treatment on breast cancer patients in Surakarta. Keywords:Herbal Usage History, Delay in Treatment, Breast Cancer

Upaya preventif Kanker Cervix dan Kanker Payudara di Masa Pandemi melalui Seminar Daring bagi Masyarakat Kota Solo dan Sekitarnya Brian Wasita; Nanang Wiyono; suyatmi suyatmi; Ratih Dewi Yudhani; Rachmi Fauziah Rahayu; Kristanto Yuli Yarso; Riza Novierta Pesik
PLACENTUM: Jurnal Ilmiah Kesehatan dan Aplikasinya Vol 9, No 1 (2021): February
Publisher : Program Studi Kebidanan Fakultas Kedokteran Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/placentum.v9i1.45483

Latar belakang dan tujuan : Kanker merupakan penyebab kematian tertinggi setelah penyakit kardiovaskuler (jantung dan stroke). Penyakit kanker banyak memakan korban meninggal karena kebanyakan baru terdeteksi saat sudah memasuki stadium lanjut sehingga sulit disembuhkan.. Pada wanita kanker yang terbanyak adalah kanker payudara dan kanker leher rahim. Oleh karena itu perlu usaha pencegahan dan deteksi dini pada kanker payudara dan kanker leher rahim apalagi pada masa pandemic dimana akses ke fasilitas kesehatan terbatas.Metode Pelaksanaan : Kegiatan pengabdian masyarakat berupa seminar daring untuk memberikan pemahaman tentang kanker payudara dan kanker leher rahim meliputi gejala-gejala awal, cara deteksi dini, pencegahan dan tatalaksana apabila sudah didapatkan kanker. Peserta yang terdaftar sejumlah 164 orang dari berbagai kalangan masyarakat dan berbagai wilayah di Indonesia. Pada hari H seminar peserta yang hadir mencapai 121 orang, peserta antusias mengikuti presentasi dan diskusi hingga akhir acara. Hasil dan Pembahasan : Setelah acara dilakukan survey tentang sikap dan pemahaman mengenai kanker payudara dan kanker leher rahim. Sebanyak 89,6 persen khawatir kalau-kalau menderita kanker dan sebanyak 96,9 % responden berusaha mencari informasi tentang kanker, dengan demikian seminar daring ini dapat menjebatani masyarakat untuk mencari informasi yang tepat tentang kanker. Dari hasil survey pemahaman tentang kanker payudara dan kanker leher rahim diperoleh nilai rata-rata 90,73 namun ada beberapa hal penting seperti penyebab kanker leher rahim, penyeberan dan factor yang berpengaruh pada kanker payudara masih belum paham (yang menjawab benar 11 s/d 20 % responden). Peserta webinar mendapatkan sertifikat dan juga doorprize berupa masker. Setelah acara seminar peserta memberikan respon positif untuk ditindaklanjuti dengan acara berikutnya,Kata kunci : penyuluhan, kanker payudara, kanker leher rahim, deteksi dini

Pengaruh Ekstrak Etanolik Lidah Buaya (Aloe vera) Terhadap Tingkat Maturasi Sel Sperma pada Testis Tikus Wistar (Rattus norvegicus) Model Sindrom Metabolik Terinduksi Nadzifah Nur Firdaus; Setiawan, Novan Adi; Budiani, Dyah Ratna; Riza Novierta Pesik
Plexus Medical Journal Vol. 3 No. 5 (2024): Oktober
Publisher : Fakultas Kedokteran, Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jhfbgj54

Pendahuluan: Sindrom metabolik memunculkan gangguan spermatogenesis, akibat adanya peningkatan ROS. Peningkatan ROS mengganggu maturasi sel sperma. Ekstrak etanolik daun lidah buaya memiliki potensi sebagai antioksidan. Tujuan penelitian ini mengetahui pengaruh ekstrak etanolik daun lidah buaya (Aloe vera) terhadap tingkat maturasi sel sperma menggunakan Johnson score. Metode: Penelitian eksperimental laboratorik dengan pretest-posttest control group design. Dua puluh lima tikus Wistar jantan dibagi menjadi 5 kelompok, masing-masing kelompok sebanyak 5 tikus. K1-kelompok kontrol, K2-kelompok sindrom metabolik, K3, K4, dan K5 adalah kelompok tikus dengan sindrom metabolik yang diberi ekstrak etanolik daun lidah buaya dosis 200, 250, dan 300 mg/KgBB/hari. Penghitungan Johnson score menggunakan perparat histopatologi dengan pengecatan Hematoxilin Eosin (HE) setelah terminasi pada hari ke-57. Analisis data menggunakan uji one-way ANOVA, dilanjutkan dengan uji post-hoc Tukey HSD, dan uji korelasi Pearson, untuk mengetahui hubungan antara dosis ekstrak etanolik Aloe vera dengan tingkat maturasi sperma. Hasil:. Berdasarkan hasil uji korelasi Pearson’s kenaikan Johnson score (tingkat maturasi sel sperma) sejalan dengan peningkatan dosis ekstrak etanolik daun lidah buaya dengan R2= 0,783. Hasil uji beda one-way ANOVA terdapat perbedaan antar kelompok p<0,05. Uji post-hoc Tukey HSD K1 secara signifikan berbeda dengan K2. K1 tidak berbeda dengan kelompok K3, K4 dan K5. K2 secara signifikan berbeda dengan K3,K4 dan K5. Rata-rata skor Johnson K1 9,92 0,05, K2 7,04±1,49, K3 8,93 0,76, K4 9,50 0,23, dan K5 9,56 0,53. Kesimpulan: Pemberian ekstrak etanolik daun lidah buaya (Aloe vera) dosis 200 mg/KgBB/hari, 250 mg/KgBB/hari dan 300 mg/KgBB/hari berpengaruh positif terhadap maturasi sperma tikus Wistar model sindrom metabolik.

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