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All Journal JURNAL PENDIDIKAN TAMBUSAI Jurnal Onoma: Pendidikan, Bahasa, dan Sastra SCRIPTA SCORE Scientific Medical Journal Narra J Jurnal Elkasista Electrician : Jurnal Rekayasa dan Teknologi Elektro Indonesian Journal of Auditing and Accounting JIMKI: Jurnal Ilmiah Mahasiswa Kedokteran Indonesia
Maulana, Rafi
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Biochemistry, Genetics & Molecular Biology Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Other

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Novel of Tuberculosis Vaccine Candidates through In Silico and In Vivo Analysis of Single Epitope Protein PE-PGRS Mycobacterium Tuberculosis Adha, Muhammad Alghifary; Maulana, Rafi; Mifindra, Rafhy
JIMKI: Jurnal Ilmiah Mahasiswa Kedokteran Indonesia Book of Abstrack RCIMS 2025
Publisher : BAPIN-ISMKI (Badan Analisis Pengembangan Ilmiah Nasional - Ikatan Senat Mahasiswa Kedokteran Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53366/jimki.vi.955

Abstract

Tuberculosis (TB) is an infectious disease that remains a serious global health threat. The use of the Bacille Calmette–Guérin (BCG) TB vaccine has so far shown unsatisfactory results due to its inconsistent and limited effectiveness. In this study, a comprehensive preclinical study pipeline was developed to design a novel single-epitope subunit vaccine targeting the PE_PGRS protein family of Mycobacterium tuberculosis. VaxiJen screened antigenic proteins, IEDB and NetMHCpan predicted B- and T-cell epitopes. Selected epitopes were assembled with linkers and an adjuvant. The construct was expressed, purified, and tested in vivo in mice for antibody and cytokine responses. The construct result showed, which had a molecular weight of 35.1 kDa and an instability score of 16.58, was found to be stable, soluble, and somewhat hydrophilic by physicochemical examination. The three-dimensional model showed a tight and stable fold that was dominated by ?-sheets and ?-helices. Strong binding affinities with MHC class I (?G = ?22.7 kcal/mol) and class II (?G = ?10.9 kcal/mol) were confirmed by molecular docking and PRODIGY studies. In the in vivo test, the single epitope exposure group had an average value of 0.090 ± 0.017. This indicates that single epitope exposure provides a significant effective antigen presentation and T-cell activation and increase in the measured parameters compared to the control group. Collectively, these findings highlight the potential of the designed single-epitope construct as a safe, stable, and immunogenic vaccine candidate against M. tuberculosis, meriting further experimental validation.
Co-Authors Adha, Muhammad Alghifary Afdhal, Fitrah Al Habsy, Muhammab Nandito Azriz, Helena Buana, Gherriandi R. Carmenita, Mutiara A. Cuandra, Kevin N. Dekki Widiatmoko Firjatullah, Muhammad F. Hanifah, Wafiq Hazmi, Auliya A. Hibatullah, Muhammad N. Ibrahim, Filzatuz Z. Ibrahim, Raihan Syah Jamsari Jamsari Kasiyanto Kristaningtyas, Nathania A. Maharani, Annisa D. Mifindra, Rafhy MUHAMMAD TEGUH, MUHAMMAD Nasri, Syahidatul A. Nayu, Balqist K. Prayogi, Norbertus M. Rahman, Awalil RK. Ramadhana, Reza Rasendriya, Daffa Z. Rauza Sukma Rita Resdiawan, Reski Rita, Rauza S. Syafaat, Mokhammad Tristan, Christopher D. Wahyu Imam Fauzi Wibisono Wahyudi, Dhyani P. Widiatmoko, Dekki Yahono, Angela S. Yenni Hayati Yenni Hayati Yenny, Satya W.