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All Journal Jurnal MIPA Indonesian Journal of Community Dedication Diagnosis: Jurnal Ilmiah Kesehatan Borneo Journal Of Pharmascientech Wal'afiat Hospital Journal Kolaborasi Jurnal Pengabdian Masyarakat WAL'AFIAT HOSPITAL JOURNAL Journal of Pharmaceutical Science and Herbal Technology Jurnal Ilmiah Mahasiswa & Penelitian Keperawatan
Muin, Rahmatullah
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Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Education Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Nursing Physics Public Health Veterinary

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Journal : Jurnal MIPA

 1 
Formulasi dan Uji In Vitro Nilai Sun Protecting Factor (SPF) Krim dari Cangkang Telur Ayam Ras Usman, Yusnita; Muin, Rahmatullah
Jurnal MIPA Vol 10, No 1 (2021)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jmuo.10.1.2021.31188

Abstract

Potensi limbah cangkang telur (CT) ayam ras di Indonesia cukup besar yakni dihasilkan + 133.703 ton per tahunnya. Penyusun utama dari cangkang tersebut adalah kalsium karbonat (CaCO3) sebesar + 98% dapat berfungsi memblok sinar ultraviolet yang akan terpapar pada kulit. Penelitian ini bertujuan untuk membuat formula dan penentuan nilai sun protecting factor (SPF) secara in vitro krim dari limbah cangkang telur ayam ras. Formula dibuat  dengan perbedaan konsentrasi cangkang telur F1(10%) dan F2 (15%).  Kemudian dilakukan evaluasi karakteristik fisik yang meliputi uji organoleptis, tipe emulsi, kriming, viskositas dan pH serta inversi fase sebelum dan setelah kondisi dipercepat selama 10 siklus. Selanjutnya ditentukan nilai SPF menggunakan spektrofotometer UV-VIS pada panjang gelombang 290-320 nm dan hasil pengukurannya dihitung menggunakan rumus SPF. Data hasil evaluasi kestabilan fisik krim dianalisis menggunakan SPSS dengan uji T parsial dengan tingkat kepercayaan 95% dan signifikansi dianggap bermakna jika P<0,05. Sedangkan nilai SPF dianalisis berdasarkan kategori proteksi tabir surya. Hasil penelitian menunjukkan F1 dan F2 memiliki stabilitas fisik yang memadai. Hasil uji nilai SPF baik CT 7500; CT 9000, F1 dan F2 menunjukkan tidak ada perbedaan kategori proteksi UV. Semua kelompok menunjukkan aktivitas uv protektif dengan kategori ekstra.The potential of chicken eggshell waste in Indonesia is quite large, which is produced + 133.703 every years. The main constituent of this shell is 98% of calcium carbonate (CaCO3) which functions to block ultraviolet light that will be exposed to the skin. This study aims to make a formula and determine the sun protecting factor (SPF) by in vitro test of cream from waste of this eggshells. The formula was made with difference concetration of  this eggshell  F1 (10%) and F2 (15%). Then the evaluation of physical characteristics includes organoleptic test, emulsion type, creaming, viscosity and pH as well as phase inversion before and after the accelerated condition for 10 cycles. Furthermore, the SPF was determined using a UV-VIS spectrophotometer at a wavelength of 290-320 nm and the measurement results were calculated using the SPF formula. The data from the evaluation of the physical stability of the cream were analyzed using SPSS with a partial T test with a confidence level of 95% and the significance was considered significant if P <0.05. Meanwhile, the SPF value was analyzed based on the sunscreen protection category. The results showed that F1 and F2 had adequate physical stability. The SPF value test results are CT 7500; CT 9000, F1 and F2 showed no difference in the UV protection category. All groups showed extra category protective UV activity.
Uji Aktivitas UV Protektif Secara In Vivo pada Krim dari Bahan Aktif Cangkang Telur Ayam Ras Menggunakan Hewan Coba Kelinci Betina Usman, Yusnita; Muin, Rahmatullah
Jurnal MIPA Vol 11, No 1 (2022)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jm.v11i1.36911

Abstract

Previously, an in vitro study of the SPF value of a cream formulation with active ingredients from broiler eggshells was conducted using UV-vis spectrophotometry at a wavelength of 290-320 nm and obtained an SPF value of 8.335 at a concentration of 9000 ppm. This study aims to continue testing the UV protective activity in vivo on creams from these active ingredients with combination of adding 5% titanium dioxide. The experimental animals used consisted of 12 female rabbits divided into 4 groups, namely (1) positive control: market product spf 15, (2) Negative control: cream base, (3) Formula I: (15% egg shell), and (4 ) Formula II: (a combination of 15% eggshell and 5% titanium dioxide). The activity test was carried out by determining (SPF) against UV-B rays in vivo in 4 groups of experimental animals by comparing the Minimum Erythema Dose (MED) value on protected skin with those not protected by sunscreen. The test results were then analyzed based on the category of sunscreen uv protection. The results showed the SPF value of Group 1; 2; 3 and 4 respectively 15.3; 0 ; 8.55 and 12.37. Group 4 has the same activity as group 1 and has better activity than group 3.
Co-Authors Ariani Ariani Aulia, Riska B, Muthmainna Bakkareng, Hasma Hasma darwis darwis, darwis Datu, Jumiati Dewi, Indra Fajriansi, Andi Indrayani, Ferna La Sakka Muthmainna B Nurpati Panaungi, Andi Panaungi, Andi Nurpati Reosi, Karina Sahdi, Anhari Usman, Yusnita wahab, masyitah Yulianah Sulaiman Zulfatma