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All Journal Jurnal Kedokteran Hewan Jurnal Medika Veterinaria
AK, M Daud
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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DETECTION OF MULTIDRUG-RESISTANT Klebsiella pneumoniae IN THE UPPER RESPIRATORY TRACT OF BROILERS AND LAYER CHICKENS FROM COMMUNITY FARM IN ACEH BESAR, INDONESIA Darniati, Darniati; Erina, Erina; Abrar, Mahdi; Zainuddin, Zainuddin; AK, M Daud
Jurnal Kedokteran Hewan Vol 19, No 2 (2025): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v19i2.45844

Abstract

This study aimed to investigate the presence of multidrug-resistant (MDR) Klebsiella pneumoniae in upper respiratory tract of broiler chickensand laying hens from community farms in Aceh Besar. Tracheal swab samples were collected and cultured on eosin methylene blue agar for bacterialisolation. Suspected isolates were identified through biochemical testing and confirmed via PCR targeting the rpoB gene. Identified isolates weretested for antibiotic susceptibility against ciprofloxacin, tetracycline, gentamicin, erythromycin, and chloramphenicol, using the disc diffusionmethod. The results revealed K. pneumoniae in 6% of broiler and 14% of laying hens, with widespread multidrug resistance. All isolates wereresistant to erythromycin (100%), gentamicin (100%), tetracycline (85%), ciprofloxacin (40%), and chloramphenicol (15%). These findingsconcluded that there has been the spread of MDR of K. pneumoniae in broiler chickens and laying hens within Aceh Besars community farms inAceh Besar, Indonesia.
Resistance Testing of Salmonella sp. Isolated from Broiler Chicken against Antibiotics Syafitri, Martiana; Erina, Erina; AK, M Daud; Ferasyi, Teuku Reza; Hamzah, Abdullah; Nazaruddin, Nazaruddin; Ismail, Ismail
Jurnal Medika Veterinaria Vol 17, No 1 (2023): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v17i1.20301

Abstract

Antibiotic resistance has become a global problem, this happens because strains of bacteria in the body are resistant to antibiotics, one of which is caused by improper use. This study aims to determine the resistance of Salmonella sp. isolated from broiler chickens to antibiotics. The method used for this study is the Kirby Bauer disc diffusion method. The research samples were eight isolates from broiler chickens collected by the Laboratory of Microbiology, Faculty of Veterinary Medicine, Universitas Syiah Kuala. This study used antibiotics amoxicillin, streptomycin, chloramphenicol, ciprofloxacin, gentamicin, and tetracycline. The data obtained are processed descriptively. The results showed five isolates (62.5%) showed multidrug resistance and very high levels of resistance to antibiotics from eight samples tested, namely tetracycline 87.5% (7/8), chloramphenicol 75% (6/8), amoxicillin 62.5% (5/8), and streptomycin 62.5% (5/8). Antibiotics that are still sensitive are gentamicin 62.5% (5/8) and ciprofloxacin 50% (4/8). Therefore, it can be concluded that Salmonella sp. has been resistant to several types of antibiotics.
Antibacterial Activity and Sensory Characteristics of Low Calories Functional Candy Contains Trigonas Honey (Trigona Itama) and Patchouli Oil (Pogostemon Cablin Benth) Juliani, Juliani; Irmayanti, Irmayanti; AK, M Daud
Jurnal Medika Veterinaria Vol 16, No 2 (2022): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v16i2.28256

Abstract

Candy is a product made from sugar which can cause health problems. The proportion of sugar with high calories can be replaced with isomalt which is low in calories, and honey which is beneficial to health. In addition, the use of synthetic flavours in candy can be replaced with essential oils such as patchouli oil. Product analysis was in the form of salivary bacterial growth inhibition and hedonic organoleptic tests to determine the samples' antibacterial activity and sensory characteristics. This research was conducted using two factors of a sugar formulation consisting of 3 levels, namely, G1, G2, and G3. The second factor was patchouli oil concentration consisting of 4 levels, namely N1, N2, N3, and N4. Data on the results of the antibacterial and hedonic activity tests were analyzed using a factorial, Completely Randomized Design (CRD). From the analysis of variance, it can be concluded that sugar formulation, patchouli oil concentration, and their interactions had a significant effect (P0.05) on the growth of salivary bacteria, patchouli oil concentration had a significant effect (P0.05) on hedonic aroma, sugar formulation had a significant effect (P0.05) P0.05) on hedonic texture and had no significant effect (P0.05) on hedonic colour and taste. The best treatment was obtained from the G2N2 sample with salivary bacterial growth of 34.50 6.02 colonies and an overall hedonic value of 3.05 (likes).
Co-Authors . Darniati Abdullah Hamzah, Abdullah Erina Erina Irmayanti Irmayanti, Irmayanti Ismail Ismail Juliani Mahdi Abrar N. Nazaruddin Syafitri, Martiana Teuku Reza Ferasyi Zainuddin, Zainuddin