Article Per Year (5 Year)
p-Index From 2020 - 2025
1.203
P-Index
This Author published in this journals
All Journal Jurnal Kesehatan Manarang Jurnal Analis Kesehatan INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY JURNAL INOVASI DAN PENGABDIAN MASYARAKAT INDONESIA
Afriansyah, Muhammad Ardi
Unknown Affiliation
Humanities Education Health Professions Immunology & microbiology Nursing Public Health Social Sciences

Published : 6 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 6 Documents
Search

 1 
Penyuluhan dan Pemeriksaan Golongan Darah Sistem ABO - Rhesus Bagi Anak-Anak dan Pengelola Panti Asuhan Sonaf Maneka Kupang, Nusa Tenggara Timur Musa, Susan Hasanah; Mutmainnah, Siti; Ayanti, Bio Putri; Dewi, Okta Yosiana; Ernanto, Aditya Rahman; Sultistyaningtyas, Ayu Rahmawati; Ethica, Stalis Norma; Afriansyah, Muhammad Ardi
JURNAL INOVASI DAN PENGABDIAN MASYARAKAT INDONESIA Vol 3 No 1 (2024): Januari
Publisher : Fakultas Kesehatan Masyarakat, Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26714/jipmi.v3i1.294

Abstract

Latar belakang: Golongan darah merupakan informasi yang penting bagi setiap individu, karena menentukan berbagai aspek medis seseorang yang meliputi antara lain keperluan transfusi darah, transplantasi organ dan kehamilan. Keberhasilan dalam berbagai aspek medis tersebut sangat ditentukan oleh kecocokan golongan darah. Hasil survei pendahuluan menunjukkan bahwa Anak-anak dan pengelola  Panti Asuhan Katolik Sonaf Maneka Kupang  banyak yang belum mengetahui manfaat mengetahui golongan darah dan belum memiliki informasi tentang golongan darahnya masing-masing. Tujuan: memberikan pengetahuan kepada anak-anak dan pengelola panti Asuhan Sonaf Manek di Kupang tentang pentingnya mengetahui golongan darah dan memberikan informasi tentang golongan darahnya masing-masing. Metode: Penyuluhan dilakukan oleh tim mahasiswa dan dosen Universitas Muhammadiyah dalam skema Pembangunan Kesehatan Masyarakat Desa (PKMD) dengan metode ceramah yang didukung penggunaan media poster. Evaluasi kegiatan penyuluhan dilakukan dengan kuesioner, sedangkan pemeriksaan golongan darah sistem ABO (A, B, AB dan O) dan Rhesus dilakukan secara langsung oleh mahasiswa Program Diploma Teknologi Laboratorium Medik. Hasil: Hasil analisis yang dilakukan menunjukkan bahwa kegiatan penyuluhan dengan media poster meningkatkan pemahaman peserta akan pentingnya pengetahuan tentang golongan darah. Kesimpulan: hasil pemeriksaan golongan darah menunjukkan bahwa anak-anak dan pengelola panti asuhan yang bergolongan darah A, B, AB, O berturut-turut 23, 43, 7, dan 27% serta seluruhnya memiliki golongan darah Rhesus Positif (Rh+). Kata kunci: media poster, panti asuhan, pemeriksaan golongan darah, pembangunan kesehatan masyarakat desa _______________________________________________________________________________ Abstract Background: Blood type is important information for every individual, as it determines various aspects of a person's medical such as the need for blood transfusions, organ transplants, and pregnancy. The compatibility of blood types largely determines success in various medical aspects. The preliminary survey results show that many children and tenants at the Sonaf Maneka Catholic Orphanage in Kupang did not know the benefits of knowing their blood type and did not yet have information about their respective blood groups. Objective: to provide knowledge to the children of the Sonaf Maneka Orphanage at Kupang about the importance of knowing their blood type and providing information about their respective blood groups. Method: Counseling was carried out by a team of students and lecturers of Muhammadiyah University in the Village Community Health Development (PKMD) scheme with the lecture method supported by media posters. Evaluation of activities carried out by questionnaires, ABO, and Rhesus system blood group examinations were carried out directly by students of the Diploma Program of Medical Laboratory Technology. Result: Results of the analysis showed that the outreach activities with posters increased participants' understanding of the importance of knowledge about blood groups. Conclusion: the results of the blood group examination showed that orphanage tenants with blood types A, B, AB, and O were 23, 43, 7, and 27% respectively and all were Rh-Positive (Rh+). Keywords: poster media, orphanage children, counseling, blood type test, village community health development
Pengaruh Sentrifugasi Spesimen Darah Anemia Terhadap Derajat Aglutinasi Pemeriksaan Golongan Darah Metode Slide Handayani, Sri Puji; Sukeksi, Andri; Afriansyah, Muhammad Ardi
Jurnal Analis Kesehatan Vol. 11 No. 1 (2022): JURNAL ANALIS KESEHATAN
Publisher : Department of Health Analyst, Politeknik Kesehatan, Kementerian Kesehatan Tanjungkarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jak.v11i1.3196

Abstract

Anemic patients often have low hemoglobin and red blood cell level, which on the slide method and ABO system blood type test is used to obtain low degree of agglutination. Addition of treatment of a centrifugation was used to obtain a stronger degree of agglutination. The purpose of this research was to determine the difference degree of agglutination between centrifuged and uncentrifuged blood specimens in anemic patients. Quasi experimental research with non-equivalent control group design was conducted in March 2022 at the Hematology Laboratory, Universitas Muhammadiyah Semarang. The research sample were 16 specimens of anemic patients for slide method and ABO system blood type test. All samples were checked for blood type testbefore being centrifuged, then re-checked after the centrifugation there only concentrated red blood cell deposit were taken with only a small amount of plasma. The data from the test were analyzed using the Kruskal Wallis method. Comparison anti-A, anti-B, and anti-AB between centrifuged and uncentrifuged blood specimens there was a significant difference (p value < 0,005 means p = 0,002). The result showed that there were effect by centrifugation for the degree of agglutination improved better in centrifuged if compared with uncentrifugedanemicpatients blood specimens on blood type test slide method. 
Efek Homogenisasi Spesimen Darah Metode Inversi Terhadap Nilai Hematokrit Fadillah, Noor; Afriansyah, Muhammad Ardi; Sukeksi, Andri; Santosa, Budi
Jurnal Analis Kesehatan Vol. 12 No. 1 (2023): JURNAL ANALIS KESEHATAN
Publisher : Department of Health Analyst, Politeknik Kesehatan, Kementerian Kesehatan Tanjungkarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jak.v12i1.3714

Abstract

Laboratory examination consists of three stages, namely pre-analytical, analytical, and post-analytic. The biggest error occurred in the pre-analytical stage, which was around 61%. Homogenization including the pre-analytical stage is the process of mixing blood and anticoagulants which are included in the pre-analytical stage. Homogenization has two ways namely manual and automatic. Inadequate homogenization can cause clots or rupture of erythrocytes/lysis and shrinkage of erythrocytes, leading to a falsely low hematocrit value. The study aimed to find out and analyze differences in hematocrit values by homogenizing the inversion method 2, 4, and 10 times. This type of research is observational analytic. The research was carried out in June - August 2021 at the Clinical Laboratory of Muhammadiyah University of Palangkaraya. The research sample consisted of 9 EDTA blood from 9 people according to the inclusion criteria. All samples were examined for hematocrit using a Hematology Analyzer Sysmex XP-300. The results showed that the average hematocrit value in 2 times inversion homogenization was 38.40%, 4 times homogenization was 38.78%, and 10 times homogenization was 39.14%. The conclusion of this study was that there was no significant effect of the inversion homogenization technique 2, 4, and 10 times on the hematocrit value (p=0.584 > 0.05).
Effect Of Donor Blood Storage Period on Hemoglobin Levels and Erythrocyte Cell Morphology Wulandari, Eka Wahyu Putri; Afriansyah, Muhammad Ardi; Sukeksi, Andri
Jurnal Kesehatan Manarang Vol 10 No 2 (2024): August 2024
Publisher : Politeknik Kesehatan Kemenkes Mamuju

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33490/jkm.v10i2.929

Abstract

Blood is a body fluid whose stability can be influenced by both internal and external factors such as storage period. Storing blood specimens for a long time can cause changes in the physiological condition of blood cells such as color, size, shape and cell formation. This study aims to determine the effect of the storage period for donor blood specimens at UTD RSUP dr. Kariadi Semarang on hemoglobin levels and erythrocyte morphology. The research design is an experiment using a modified Time Series design with a pre and posttest control group design. The samples in this study were CPDA-1 (Citrate Phosphate Dextrose Adenine) donor blood specimens at the Blood Transfusion Unit of RSUP dr. Kariadi Semarang. Observation of erythrocyte cell morphology using blood smear preparations (SHD). Primary data was taken from the results of measurements of hemoglobin levels and observations of erythrocyte cell morphology with blood specimens examined directly (day 0) and storage days 1, 2, and 3. Analysis of research data used the One-way Anova statistical test. The results of the One-Way Anova test showed that there was a significant difference in hemoglobin levels on the 3rd day of donor blood storage (p-value 0.000). The results of the evaluation of erythrocyte cell morphology showed that there were abnormalities in crenation morphology on the 3rd day of storage. Blood specimens should be processed immediately after they leave the body because the condition of the blood cells is still stable and optimal for use in laboratory tests.
In vitro anticoagulant activity of crude fibrinolytic protease HSFT-2 as an alternative anticoagulant agent Afriansyah, Muhammad Ardi; Rahajoe, Toeti; Pranindira, Raksi
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 7 No 1 (2025): Advances in biomarkers, therapeutics, and probiotics: Recent updates in medical l
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v7i1.5758

Abstract

Thrombotic disorders are one of the leading causes of death in cardiovascular patients and contribute to the incidence of non-infectious diseases. The search for new anticoagulant agents to overcome the limitations associated with existing anticoagulant therapies is increasing, especially those derived from natural and microbial sources. Staphylococcus hominis HSFT-2 is a Gram-positive coccus isolated from rusip Holothutia scabra commonly known as sand sea cucumber. Fibrinolytic protease HSFT-2 has been reported to be able to degrading fibrin, which has shown several characteristics of fibrinolytic and clot-lysis activities. This study aims to evaluate the anticoagulant activity of crude fibrinolytic protease HSFT-2 in vitro as potential alternative anticoagulant agents. The research design was completely randomized, with a sample size of 21 participants calculated using the Federer formula. The material used was crude protease derived from S. hominis cultured in Skim Milk Broth. Various tests, including blood routine tests, platelet aggregation tests, and blood smear evaluations, were conducted to assess its effectiveness as an anticoagulant. Whole blood samples were divided into three groups: a positive control, a negative control, and a group treated directly with 100 µL of crude fibrinolytic protease. The normality of the data was evaluated using the Kruskal-Wallis test, while differences between groups were analyzed with one-way ANOVA. The one-way ANOVA results from the blood routine tests indicated a significant difference between the control and the fibrinolytic protease HSFT-2 (p<0.05). The platelet aggregation percentage decreased from 75.85% in the control group to 21.75% with the addition of crude protease HSFT-2. Furthermore, the blood smear evaluations revealed no abnormalities in cell formation when HSFT-2 protease was used, compared to samples without the addition of EDTA anticoagulant. In conclusion, the fibrinolytic protease HSFT-2 exhibits anticoagulant activity; however, it does not yet meet the standards required for use as an anticoagulant in routine blood tests. Future work should focus on the purification and characterization of the protease to enhance its anticoagulant potential for laboratory diagnostic applications.
Exploration and molecular identification of proteolytic bacteria from rusip pacific oyster (Crassostrea gigas) as anticoagulant agent candidates Afriansyah, Muhammad Ardi; Sudarwin; Dewi, Sri Sinto; Akbar, Gusti Dimas Refian
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 7 No 1 (2025): Advances in biomarkers, therapeutics, and probiotics: Recent updates in medical l
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v7i1.6083

Abstract

The marine symbiont Staphylococcus epidermidis strain CGF-6, a protease-producing bacterium, has been successfully isolated from Rusip Pacific Oyster (Crassostrea gigas). S. epidermidis is a non-spore-forming, Gram-positive coccus commonly found in marine environments due to their ability to tolerate high salinity. The aim of this study was to identify proteolytic bacteria from Rusip fermented C. gigas as potential candidates for the development of anticoagulant agents. Bacterial isolation was performed through the fermentation process of Rusip. After seven days, bacterial colonies were purified three times using Nutrient Agar. The selection of proteolytic bacterial was conducted qualitatively using a skim milk agar medium. The bacterial isolates exhibiting the highest protease activity were identified through 16S rRNA gene sequencing using universal primers Bact 27F and UniB 1492R. Phylogenetic tree analysis, conducted with the MEGA X program, helped determine the relationships between species. Out of the 18 bacterial isolates obtained from the Rusip fermentation of C. gigas, three isolates (CGF-1, CGF-6, and CGF-11) exhibited hydrolysis zones around their colonies on skim milk agar, indicating protease activity.  Among these, isolate CGF-6 showed the highest proteolytic index of 0.5 and was identified as Staphylococcus epidermidis strain CGF-6. S. epidermidis strain CGF-6 has the potential to serve as a valuable source of protease production for the development of anticoagulant agents. However, further studies, including enzyme characterisation, optimisation, and both in vitro and in vivo anticoagulant activity tests, are necessary to assess the efficacy and safety of this enzyme as a candidate for anticoagulant agents.
Co-Authors Akbar, Gusti Dimas Refian Andri Sukeksi Ayanti, Bio Putri Budi Santosa Budi Santosa Dewi, Okta Yosiana Ernanto, Aditya Rahman Fadillah, Noor Handayani, Sri Puji Musa, Susan Hasanah Pranindira, Raksi Rahajoe, Toeti Siti mutmainnah Sri Sinto Dewi Stalis Norma Ethica Sudarwin Sultistyaningtyas, Ayu Rahmawati Wulandari, Eka Wahyu Putri