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All Journal agriTECH Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Indonesian Journal of Chemistry Indonesian Food and Nutrition Progress Journal of Functional Food and Nutraceutical Journal of Applied Agricultural Science and Technology

Setyaningsih, Widiastuti

Department Of Food And Agricultural Product Technology, Faculty Of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia

Author-ID : 1813628

Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Nursing

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Articles

Experimental Design-Assisted Optimization of Chromatographic Method for the Simultaneous Quantitation of Phenolic Compounds in Dried Flowers Extract Thorvaldsson , Mira; Mutmainah , Nurul; Briliantama , Asadin; Rahmawati , Sitti; Priyanto, Ashari; Pargiyanti; Setyaningsih, Widiastuti
Journal Akademik Universitas Swiss German Vol. 3 No. 2 (Feb 2022)
Publisher : Swiss German University & Perhimpunan Penggiat Pangan Fungsional dan Nutrasetikal Indonesia (P3FNI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33555/jffn.v3i2.88

This research aimed to develop and validate a reversed phase-high performance liquid chromatography method to determine phenolic compounds in dried flowers extract simultaneously. The research was divided into two parts: (1) optimization of the separation condition employing a Box Behnken design, and (2) validation test including assessment for the precision, accuracy, and method applicability of a High-Performance Liquid Chromatography (HPLC) coupled with Diode Array Detector (DAD). The studied factors for the optimization of the separation condition were flow rate (0.8−1.2 ml min-1), percentage of the mobile phase at the beginning (0−20% phase B), and end (70−100% phase B) of the gradient program. It was statistically evinced that the chromatographic resolutions (Rs>1.0) indicated acceptable separation for protocatechuic acid, p-hydroxybenzoic acid, protocatechuic aldehyde, vanillic acid, p-coumaric acid, and ferulic acid. A fast separation method (8.00 min) was achieved by applying the optimum condition of a flow rate of 1 mL min-1, mobile phase composition of 20% acidified methanol at the beginning, and 100% acidified methanol at the end of the gradient program. The validation was then performed for the developed method assuring high precision and accuracy. Additionally, the HPLC-DAD method was successfully applied to determine the phenolic compounds in three dried flower extracts revealing that the method was reliable for routine analyses.

Optimasi Sintesis Hydroxypropyl Methyl Cellulose (HPMC) dari Kulit Koro Pedang Putih (Canavalia ensiformis L. (DC) Yanti Nopiani; Agnes Murdiati; Widiastuti Setyaningsih
agriTECH Vol 41, No 4 (2021)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Kulit koro pedang putih dapat digunakan sebagai sumber selulosa. Salah satu alternatif untuk meningkatkan aplikasi selulosa adalah dengan memodifikasi selulosa menjadi produk turunan selulosa yaitu Hydroxypropyl Methyl Cellulose (HPMC). Tujuan dari penelitian ini adalah melakukan optimasi terhadap sintesis dan karkaterisasi HPMC dari selulosa kulit koro pedang putih. Proses optimasi didahului dengan kajian literatur untuk menentukan kisaran titik percobaan dengan variabel terikat berupa molar subtitusi (MS) dan Derajat Subtitusi (DS). Diperoleh titik percobaan dengan variasi konsentrasi NaOH (5, 22,5, dan 40%), variasi Dimetil Sulfat (DMS) (40, 80, dan 120%), dan variasi Proilen Oksida (PO) (80, 120, dan 160%). Kemudian optimasi sintesis HPMC dilakukan dengan mengunakan Box-Behnken design (BBD) lalu dianalisis menggunakan Response Surface Methodology (RSM) Berikutnya HPMC dikarakterisasi meliputi molar subtitusi (MS), Derajat Subtitusi (DS), water holding capacity (WHC), oil holding capacity (OHC), lightness, rendemen, kristalinitas dan spektra FT-IR untuk mengetahui gugus fungsi HPMC. Hasil optimasi sintesis HPMC dari selulosa kulit koro pedang putih berdasakan RSM diperoleh titik optimum pada konsentrasi NaOH 23,11%, DMS 43,4% dan PO 81,8%. dengan karakterisasi kadar air 9,04% (wb); MS 0,15; DS 1,18; WHC 2,20 g/g; OHC 2,09 g/g; lightness 90,93; rendemen 114,78% dan kristalinitas 64%. Spektra FT-IR HPMC koro pedang putih terbaca pada bilangan gelombang 2924 cm-1 (CH dan CH2 Streching), 1373 cm-1 (CH3 Bonding), 1118 cm-1 (C-O-C), 1319 cm-1 (O-H Plane Bonding) dan 848, 68 cm-1 (C-O-C pada 1,4 β glikosidic linkage) yang merupakan ciri khas dari gugus fungsional HPMC.

An Information and Marketing System for Agrocomplex Sector Based on Reliable Digital Technology as a Supporting Capacity for Food Security in a Force Majeure Event Endang Sulistiyaningsih; Valentina Dwi Suci Handayani; Widiastuti Setyaningsih; Wahdan Fitriya; Arif Wahyu Widada; Candra Aryudiawan; Taufan Alam; Imas Masithoh Devangsari
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 7, No 3 (2021): September
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

The pandemic situation experienced by the world are currently threatening food security in various regions including Indonesia. In these regions, the flow of traffic in the agricultural and fisheries sectors, from the means of production to the distribution of the harvest, is choked up due to social restrictions in various parts of the region. As a strategic step to reduce the impact on the agricultural sector, it is necessary to accelerate information to support the stability of production and also to distribute production results. Digital media in agriculture are needed to provide education with a variety of contents, such as technical production, marketing, and agricultural finance information. The Faculty of Agriculture of Universitas Gadjah Mada (UGM) has supported digital information dissemination through the Desa Apps application. Desa Apps aims to be an application platform that provides agricultural extension and develops digital farmer communities. The information that has been carried out by Desa Apps so far includes agrocomplex sector (agriculture, animal husbandry and fisheries) problems related to cultivation methods and obstacles faced in the land such as attack by plant pest and disease. As a digital media, the Desa Apps has educated farmer and aquaculturists. Optimization needs to be done to improve the performance of Desa Apps as a digital media, one of which is the marketing system that this application actually has. This study evaluates whether farmers and aquaculturists under the auspices of the Faculty of Agriculture are interested in being able to get education and at the same time be able to market their product directly to consumers with one application, the Desa Apps. The findings of the study showed that the pandemic period greatly affected the incomes of the assisted target community, and they required media that can help market their product. The decline in turnover due to pandemic conditions reached 70% with an average decrease of 39.2%. The method of selling products has changed from conventional to online sales by 20% of the total respondents. Changes in sales methods occurred due to distribution limitations due to regional and activity restrictions during the pandemic. Therefore, it is necessary to optimize the Desa Apps application as a digital media to support food security.

The Use of Chemometrics for Classification of Sidaguri (Sida rhombifolia) Based on FTIR Spectra and Antiradical Activities Abdul Rohman; Asefin Nurul Ikhtiarini; Widiastuti Setyaningsih; Mohamad Rafi; Nanik Siti Aminah; Muhamad Insanu; Irnawati Irnawati; Djoko Santosa
Indonesian Journal of Chemistry Vol 21, No 6 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.64360

Sidaguri (Sida rhombifolia) is one of the herbal components used in traditional medicine. The application of chemometrics in the standardization of herbal medicine is common. The objective of this study was to classify Sidaguri from different regions based on FTIR spectra with chemometrics of principal component analysis (PCA) and to correlate the antioxidant activities with FTIR spectra using the multivariate calibration of partial least square regression (PLSR). The extraction of Sidaguri powder was performed using ultrasound-assisted extraction (UAE) at optimum conditions. The obtained extracts were subjected to antiradical scavenging activities using DPPH (2,2’-diphenyl-1-picrylhydrazyl) and ABTS (2,2′-azinobis-3-ethylbenzothiazoline-6-sulfonic acid) radicals. The PCA result shows that Sidaguri from different regions could be separated using 14 wavenumbers of FTIR spectra based on the PCA's loading plot. PLSR regression using the second derivative FTIR spectra at wavenumbers of 3662–659 cm–1 could predict radical scavenging activities (RSA) of Sidaguri with R2 values of 0.9636 and 0.9024 for calibration and validation models, with RMSEC and RMSEP values of 1.45% and 2.65%, respectively. It can be concluded that FTIR spectra treated by PCA were reliable for classifying Sidaguri from different regions. At the same time, PLSR was accurate and precise enough to predict the RSA of Sidaguri.

Oligo-Glucomannan Production from Porang (Amorphophallus oncophyllus) Glucomannan by Enzymatic Hydrolisis Using β-Mannanase Anggela Anggela; Widiastuti Setyaningsih; Santad Wichienchot; Eni Harmayani
Indonesian Food and Nutrition Progress Vol 17, No 1 (2020)
Publisher : Indonesian Association of Food Technologists

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ifnp.57217

Porang (Amorphophallus oncophyllus) is an indigenous tuber of Indonesia that rich in glucomannan. An alternative approach to produce porang oligo-glucomannan (POG) as prebiotic from porang glucomannan (PGM) was made by enzymatic hydrolysis using β-mannanase. This study aimed to produce POG under optimal conditions by controlled enzymatic hydrolysis process. The PGM flour contained 96.12% of indigestible carbohydrates. The optimum condition of enzymatic hydrolysis producing the highest reducing sugar was as follows: temperature 37 °C, pH 5.5, a ratio of enzyme to the substrate (E/S) 1:1000, and reaction time 4 h. HPLC analysis confirmed that 99.45% of the resulting POG consisted of oligosaccharides with a degree of polymerization (DP) 3. Hence, the PGM utilized in this study has been proven as a potential substrate for POG production. Additionally, the resulting POG was considered as a functional ingredient due to has prebiotic potential.

Effect of Different Coagulants and Various Concentrations on Quality of Jack Bean Tofu Rini Yanti; Widiastuti Setyaningsih; Priyanto Triwitono; Richa Yuniansyah; Ermi Saraswati Maha Admi
Journal of Applied Agricultural Science and Technology Vol. 6 No. 1 (2022): Journal of Applied Agricultural Science and Technology
Publisher : Green Engineering Society

Jack bean (Canavalia ensiformis), Indonesian local bean, has a high content of protein and great potential either for substituting or overcoming the shortage of imported soybean in tofu production. However, an appropriate coagulant in a proper amount is needed to make tofu with acceptable characteristics. This study set out to investigate the appropriate type and concentration of coagulant. The experiment was carried out by using GDL (glucono delta lactone) and nigari as coagulants with concentration levels of 0.25; 0.5; 0.75 and 1%. Some quality parameters such as yield, color, texture (hardness, cohesiveness, chewiness, springiness), and microscopic structure of tofu were observed. Consumer preference tests on aroma, color, taste, texture, and overall acceptability were directed to determine tofu with the best characteristics. The result showed nigari at concentration level 0.75% delivered the most preferred tofu for consumers.

Enhancement in Bioaccessibility and Bioavailability of Phenolic Compounds during Black Glutinous Rice Tape Fermentation Azkia, Mita Nurul; Setyaningsih, Widiastuti; Mayangsari, Yunika; Cahyanto, Muhammad Nur
agriTECH Vol 44, No 4 (2024)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/agritech.92729

Black glutinous rice (Oryza sativa var. glutinosa) tape fermented with various yeast, mold, and bacteria is often rich in phenolics compounds and can contribute positively to health through its antioxidants activity. Despite the potential, these compounds have limited bioavailability value due to their structure, degree of glycosylation or polymerization, and interactions with other components. Therefore, this study aims to determine the effect of fermentation on bioavailability and bioaccessibility of phenolics compounds in black glutinous rice tape. During the procedures, cooked black glutinous rice was inoculated with ragi tape for 72 hours. Sampling was then performed every 24 hours to analyze bioaccessibility of phenolics compounds, flavonoids, and antioxidants activity. Subsequently, absorption was carried out using an everted gut sac model. The results showed that phenolics compounds were released from the food matrix during gastric and small intestine digestion. Fermentation was shown to increase the content of accessible phenolics compounds from 19.89% to 27.31%, flavonoids from 68.88% to 81.72%, and antioxidants activity from 13.56% to 22.89%. During fermentation, the highest increments were obtained after 72 hours, with 27.31% for total phenolics compounds, 81.72% for flavonoid compounds, and 22.89% for antioxidants activity. The products obtained after 72 hours of fermentation exhibited significantly highest absorption, but no significant differences were observed between the duodenum and ileum segments. The absorption of these compounds in the jejunum from the extract was significantly higher in fermented samples. Therefore, fermentation significantly enhanced bioavailability of phenolics compounds in black glutinous rice tape.

Ultrasound-Assisted Extraction of Patin Fish Oil: Optimization Using Response Surface Methodology – Box Behnken Design Putri, Anggita Rosiana; Setyaningsih, Widiastuti; Palma, Miguel; Fernandez, Ceferino Carrera; Rohman, Abdul; Riyanto, Sugeng; Jang, Young Pyo
Indonesian Journal of Chemistry Vol 25, No 5 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.105223

The oil extracted from Pangasius micronemus, commonly known as patin fish, is highly regarded for its omega-3 and omega-6 fatty acids content, which offer numerous health benefits. Producing high-quality patin fish oil (PFO) rich in these essential fatty acids requires an optimized extraction process. This study utilized ultrasound-assisted extraction (UAE) combined with response surface methodology – Box Behnken Design (RSM-BBD) to optimize the extraction of PFO. Five variables were examined: temperature (30–60 °C), solvent composition (n-hexane in isopropanol, 30–90%), amplitude (30–90%), solvent-to-sample ratio (10:1–20:1), and cycle duration (0.2–0.8 s−1). The analysis identified solvent composition, amplitude, and solvent-to-sample ratio as significant factors influencing the response value (p < 0.005). Optimal UAE conditions were achieved at a temperature of 59 °C, solvent composition of 42%, amplitude of 41%, solvent-to-sample ratio of 20:1, cycle duration of 0.8 s−1, and an extraction time of 25 min. Fatty acid profiling revealed that PFO extracted using UAE contained omega-3 fatty acids, including α-linolenic acid (ALA) at 1.36% and eicosapentaenoic acid (EPA) at 0.46%, as well as omega-6 fatty acids, namely linoleic acid (LA) at 19.06% and arachidonic acid (AA) at 0.85%. These results demonstrate the efficiency of UAE in extracting high-quality PFO.

Co-Authors Abdul Rohman Abdul Rohman Agnes Murdiati Anak Agung Istri Sri Wiadnyani Anggela Anggela Anggita Rosiana Putri Arif Wahyu Widada Asefin Nurul Ikhtiarini Azkia, Mita Nurul Briliantama , Asadin Candra Aryudiawan Djoko Santosa Endang Sulistiyaningsih Eni Harmayani Ermi Saraswati Maha Admi Fernandez, Ceferino Carrera Imas Masithoh Devangsari, Imas Masithoh Irnawati Irnawati Jang, Young Pyo Mayangsari, Yunika Mohamad Rafi Muhamad Insanu Muhammad Nur Cahyanto Mutmainah , Nurul Nanik Siti Aminah Nopiani, Yanti Palma, Miguel Pargiyanti Priyanto Triwitono Priyanto, Ashari Richa Yuniansyah Rini Yanti Santad Wichienchot Sugeng Riyanto Taufan Alam Thorvaldsson , Mira Valentina Dwi Suci Handayani Wahdan Fitriya Wahdan Fitriya

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