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All Journal agriTECH Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Indonesian Journal of Chemistry Indonesian Food and Nutrition Progress Journal of Functional Food and Nutraceutical Journal of Applied Agricultural Science and Technology
Setyaningsih, Widiastuti
Department Of Food And Agricultural Product Technology, Faculty Of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Nursing

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Optimasi Sintesis Hydroxypropyl Methyl Cellulose (HPMC) dari Kulit Koro Pedang Putih (Canavalia ensiformis L. (DC) Yanti Nopiani; Agnes Murdiati; Widiastuti Setyaningsih
agriTECH Vol 41, No 4 (2021)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (58.309 KB) | DOI: 10.22146/agritech.42926

Abstract

Kulit koro pedang putih dapat digunakan sebagai sumber selulosa. Salah satu alternatif untuk meningkatkan aplikasi selulosa adalah dengan memodifikasi selulosa menjadi produk turunan selulosa yaitu Hydroxypropyl Methyl Cellulose (HPMC). Tujuan dari penelitian ini adalah melakukan optimasi terhadap sintesis dan karkaterisasi HPMC dari selulosa kulit koro pedang putih. Proses optimasi didahului dengan kajian literatur untuk menentukan kisaran titik percobaan dengan variabel terikat berupa molar subtitusi (MS) dan Derajat Subtitusi (DS). Diperoleh titik percobaan dengan variasi konsentrasi NaOH (5, 22,5, dan 40%), variasi Dimetil Sulfat (DMS) (40, 80, dan 120%), dan variasi Proilen Oksida (PO) (80, 120, dan 160%). Kemudian optimasi sintesis HPMC dilakukan dengan mengunakan Box-Behnken design (BBD) lalu dianalisis menggunakan Response Surface Methodology (RSM) Berikutnya HPMC dikarakterisasi meliputi molar subtitusi (MS), Derajat Subtitusi (DS), water holding capacity (WHC), oil holding capacity (OHC), lightness, rendemen, kristalinitas dan spektra FT-IR untuk mengetahui gugus fungsi HPMC. Hasil optimasi sintesis HPMC dari selulosa kulit koro pedang putih berdasakan RSM diperoleh titik optimum pada konsentrasi NaOH 23,11%, DMS 43,4% dan PO 81,8%. dengan karakterisasi kadar air 9,04% (wb); MS 0,15; DS 1,18; WHC 2,20 g/g; OHC 2,09 g/g; lightness 90,93; rendemen 114,78% dan kristalinitas 64%. Spektra FT-IR HPMC koro pedang putih terbaca pada bilangan gelombang 2924 cm-1 (CH dan CH2 Streching), 1373 cm-1 (CH3 Bonding), 1118 cm-1 (C-O-C), 1319 cm-1 (O-H Plane Bonding) dan 848, 68 cm-1 (C-O-C pada 1,4 β glikosidic linkage) yang merupakan ciri khas dari gugus fungsional HPMC.
Enhancement in Bioaccessibility and Bioavailability of Phenolic Compounds during Black Glutinous Rice Tape Fermentation Azkia, Mita Nurul; Setyaningsih, Widiastuti; Mayangsari, Yunika; Cahyanto, Muhammad Nur
agriTECH Vol 44, No 4 (2024)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/agritech.92729

Abstract

Black glutinous rice (Oryza sativa var. glutinosa) tape fermented with various yeast, mold, and bacteria is often rich in phenolics compounds and can contribute positively to health through its antioxidants activity. Despite the potential, these compounds have limited bioavailability value due to their structure, degree of glycosylation or polymerization, and interactions with other components. Therefore, this study aims to determine the effect of fermentation on bioavailability and bioaccessibility of phenolics compounds in black glutinous rice tape. During the procedures, cooked black glutinous rice was inoculated with ragi tape for 72 hours. Sampling was then performed every 24 hours to analyze bioaccessibility of phenolics compounds, flavonoids, and antioxidants activity. Subsequently, absorption was carried out using an everted gut sac model. The results showed that phenolics compounds were released from the food matrix during gastric and small intestine digestion. Fermentation was shown to increase the content of accessible phenolics compounds from 19.89% to 27.31%, flavonoids from 68.88% to 81.72%, and antioxidants activity from 13.56% to 22.89%. During fermentation, the highest increments were obtained after 72 hours, with 27.31% for total phenolics compounds, 81.72% for flavonoid compounds, and 22.89% for antioxidants activity. The products obtained after 72 hours of fermentation exhibited significantly highest absorption, but no significant differences were observed between the duodenum and ileum segments. The absorption of these compounds in the jejunum from the extract was significantly higher in fermented samples. Therefore, fermentation significantly enhanced bioavailability of phenolics compounds in black glutinous rice tape.
Co-Authors Abdul Rohman Abdul Rohman Agnes Murdiati Anak Agung Istri Sri Wiadnyani Anggela Anggela Anggita Rosiana Putri Arif Wahyu Widada Asefin Nurul Ikhtiarini Azkia, Mita Nurul Briliantama , Asadin Candra Aryudiawan Djoko Santosa Endang Sulistiyaningsih Eni Harmayani Ermi Saraswati Maha Admi Fernandez, Ceferino Carrera Imas Masithoh Devangsari, Imas Masithoh Irnawati Irnawati Jang, Young Pyo Mayangsari, Yunika Mohamad Rafi Muhamad Insanu Muhammad Nur Cahyanto Mutmainah , Nurul Nanik Siti Aminah Nopiani, Yanti Palma, Miguel Pargiyanti Priyanto Triwitono Priyanto, Ashari Richa Yuniansyah Rini Yanti Santad Wichienchot Sugeng Riyanto Taufan Alam Thorvaldsson , Mira Valentina Dwi Suci Handayani Wahdan Fitriya Wahdan Fitriya