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All Journal HAYATI Journal of Biosciences Jurnal MIPA Wikrama Parahita : Jurnal Pengabdian Masyarakat Bioma : Jurnal Biologi Indonesia Health Information : Jurnal Penelitian

Supiyani, Atin

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Author-ID : 1816990

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Engineering Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences

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Title

Uji Efektivitas Vaksin Flu Burung Subtipe H5N1 pada Ayam Kampung di Legok, Tangerang, Banten Janovie, Arria; Rusdi, Rusdi; Supiyani, Atin
Bioma Vol. 10 No. 2 (2014): Bioma
Publisher : LPPM Universitas Negeri Jakarta

Abstract The aim of the study was to determine the effectiveness of Avian Flu Vaccine Subtype H5N1 on native chicken. There were several inclusion criteria to selected the chicks. The criteria were just native chicken in Legok, at least 8 weeks old and never been had previous vaccine program. Total of 20 chickens were used as samples. All chicken was split in two cages, cage A contained 10 chickens were treated with vaccine and cage B contained 10 chickens as a control. Then 0.5 mL of vaccine administered intramuscularly in the chicken breast. The effectiveness of vaccination determined by measuring antibody titers before and 3 weeks after vaccination program. Measurement of antibody titers obtained by HI (Hemagglutination Inhibition) test method. Result showed that antibody titers of native chicken increased after vaccination program. There were 70% of samples that have been vaccinated could be protected against the virus. Three chickens as a control had positive titers prior to vaccination with a range of 21 - 22. Key Word : test of effectiveness, homologue killed vaccine, antibody titers.

PENGAMATAN AKTIVITAS TIKUS WISTAR JANTAN DENGAN ALAT OPTO- VARIMEXÂ® PADA KONDISI DIET TINGGI MINYAK TRANS MASYITA, NURUL; SANTOSO, KOEKOEH; KUSUMORINI, NASTITI; SATYANINGTIJAS, ARYANI SISMIN; SUPIYANI, ATIN
Bioma Vol. 11 No. 1 (2015): Bioma
Publisher : LPPM Universitas Negeri Jakarta

ABSTRACT Excessive energi consumption of high-fat diet increase the bodyâ€™s adipose tissue which also affect the increased secretion of leptin, this is because leptin secretion is proportional to the mass of adipose tissue. Leptin activates the lateral hypothalamus as the center of hunger and feeding activity. The aim of this study was to obtain the relationship between high trans fat feeding on male Wistar rats and feeding activity. This method uses male Wistar rats (Rattus norvegicus) as an animal model. Sixteen rats were grouped into 4 treatment groups. The first group was given additional feed 20% trans fat, the second group was given additional feed 40% trans fat, a third group was given additional feed 60% trans fat, and the last as a control group. This feed was given once a day for 70 days. Observation of activities perform using Opto-varimexÂ® auto-track system ver. 4.31. The results showed an corellation between activity based doses of trans fat. Keywords: Opto-varimexÂ®, Trans fat, Activity, Wistar rat.

DETERMINASI PEMBERIAN MINYAK TRANS TERHADAP STRES OKSIDATIF PADA TIKUS GALUR WISTAR DENGAN MALONDIALDEHIDA PANKREAS SEBAGAI INDIKATOR Mawali, Maharja; Santoso, Koekoeh; Kusumorini, Nastiti; Satyaningtijas, Aryani Sismin; Supiyani, Atin
Bioma Vol. 11 No. 2 (2015): Bioma
Publisher : LPPM Universitas Negeri Jakarta

ABSTRACT Trans fatty acids (TFAs) are the entire unsaturated fatty acids containing one or more double bonds isolated in trans configuration believed as predisposing factor of oxidative stress, excluding conjugated double bond fatty acids. Malondialdehyde (MDA) is a product of lipid peroxidation used as oxidative stress marker. The objective of this study was to see the correlation between TFAs containing-oil (consists of 3-6% TFAs) intake at various administration doses and pancreatic MDA levels in Wistar rats. Twelve rats were grouped according to administration doses (20%, 40%, 60% of given feed total energy and control group). TFAs containing-oil was administered orally once a day for 70 days at given doses by force feeding. Pancreatic MDA were measured using TBARS assay adapted from Singh et al. (2006). Increase of TFAs containing-oil administration dose caused insignificant increase of pancreatic MDA level. Keywords: trans fatty acids, oxidative stress, malondialdehyde, Wistar rats, palm oil

DETERMINASI PEMBERIAN SUKROSA TERHADAP KADAR SGPT DAN SGOT TIKUS GALUR WISTAR SEBAGAI INDIKATOR FUNGSI HATI: Determination of High Sucrose Intake to SGPT and SGOT Concentration in Wistar Rats As Indicator of Liver Function. Andayani, Prawita Lintang; Santoso, Koekoeh; Kusumorini, Nastiti; Satyaningtijas, Aryani Sismin; Supiyani, Atin
Bioma Vol. 12 No. 1 (2016): Bioma
Publisher : LPPM Universitas Negeri Jakarta

The aim of the study was to determine the correlation between sucrose intake at various administration doses to SGPT and SGOT level in Wistar rats. SGPT and SGOT level in blood serum were used as parameter of liver function. Twelve rats were grouped according to administration doses (20%, 40%, 60% of given feed total energy and control group). Sucrose was administered orally once a day for 70 days at given doses by force feeding. SGPT dan SGOT level were measured using SGPT and SGOT Test Kit and read using spectrophotometer. The result indicated that increased administration dose caused a significant increase of SGPT and SGOT level.

RESPON AKTIVITAS TIKUS WISTAR JANTAN AKIBAT KONDISI DIET TINGGI SUKROSA DIUKUR MENGGUNAKAN PEREKAM AKTIVITAS: Activities Response Measurement on Male Wistar Rats With High Sucrose Diet Using Activity monitoring device Wijaya, Yanuar Restu; Santoso, Koekoeh; Isdoni, Isdoni; Supiyani, Atin
Bioma Vol. 12 No. 2 (2016): Bioma
Publisher : LPPM Universitas Negeri Jakarta

Excessive energi consumption play a role in increasing blood glucose levels (hyperglycemia), due to the inability of the hormone insulin to compensate the high blood glucose levels. Blood glucose receptor found in the hypothalamus of VMH (Ventro Medial Hypothalamus) and LH (the Lateral Hypothalamus) is able to detect and change the settings for the feeding activity. The aim of this study was to obtain the relationship between high sucrose feeding on male Wistar rats of behavior and activity. This method uses Wistar rats (Rattus norvegicus) as an animal model. Sixteen rats were grouped into 4 treatment groups. The first group was given additional feed 20% sucrose, the second group was given additional feed 40% sucrose, a third group was given additional feed 60% sucrose, and the last as a control group. This feed given continuously for 70 days.Observation of activities conducted using Opto-varimexÂ® auto-track system ver.4.31. The results showed an corellation between activity based doses of sucrose.

STUDI PERILAKU HARIAN DAN KESEJAHTERAAN MONYET HITAM SULAWESI (Macaca nigra Desmarest, 1822) DI PUSAT PRIMATA SCHMUTZER Octavia, Desy; Komala, Ratna; Supiyani, Atin
Bioma Vol. 13 No. 1 (2017): Bioma
Publisher : LPPM Universitas Negeri Jakarta

Monyet hitam sulawesi merupakan primata endemik yang hampir punah. Konservasi ex situ monyet hitam sulawesi harus memperhatikan segala aspek yang berhubungan dengan kehidupan hewan tersebut, termasuk aspek perilaku dan kesajahteraan hewan. Penelitian ini bertujuan untuk mengetahui perilaku harian monyet hitam sulawesi berdasarkan jenis kelamin dan umur, serta tingkat kesejahteraannya di Pusat Primata Schmutzer (PPS). Metode yang digunakan dalam penelitian ini adalah metode deskriptif dengan teknik observasi. Analisis data perilaku harian monyet hitam sulawesi berdasarkan jenis kelamin dan umur dilakukan dengan uji Mann- Whitney, sedangkan analisis data tingkat kesejahteraan hewan dilakukan secara deskriptif. Hasil penelitian menunjukan bahwa terdapat perbedaan perilaku harian monyet hitam sulawesi berdasarkan jenis kelamin, yaitu pada perilaku bergerak, mencari makan, istirahat, dan sosial. Hasil penelitian juga menunjukan terdapat perbedaan perilaku harian monyet hitam sulawesi berdasarkan umur, yaitu pada perilaku bergerak, istirahat, dan sosial. Berdasarkan hasil analisis, tingkat kesejahteraan monyet hitam sulawesi di PPS termasuk dalam kriteria baik.

EFEK PROBIOTIK DAN SELUBIOSE TERHADAP VOLATILE FATTY ACIDS (VFA) DAN NH3 RUMINAL DOMBA GARUT Supiyani, Atin
Bioma Vol. 13 No. 2 (2017): Bioma
Publisher : LPPM Universitas Negeri Jakarta

The composition of feed can improve and optimize the fermentation in sheep rumen. The purpose of this study is to determine the effect of probiotic and cellobiose to rumen fermentation of sheep. Four adult (weight Â±13.5 kg) rumen fistulae sheep were used. The fed given were King grass (Pennisetum purpureum), rice bran and soybean meal that are protected by formaldehyde 0.3% as base feed. Fed treatment were probiotics (0,5% and 1%) and cellobiose (1 ppm and 3 ppm). Parameters measured were pH, N-NH3 and VFA concentration of rumen fluid at 0, 2, 6, 12, and 24 hours after feeding with Completely Randomized Design Factorials 4x5 and continued with Duncan test (Î±=0,05). The ruminal pH range for all treatments between 6.27 - 6.89. The maximum N-NH3 concentration value has been reached at 2 hours after feeding 12.25-18.75 mM. At 0 hours, the total VFA concentration was at an average value of 294.91 mg% and then increased at 2-6 hours reaching its maximum value in the range 661.97-767.70 mg% (p<0.05). The addition of probiotics and cellobiose can optimize rumen fermentation of sheep.

KAJIAN KESEJAHTERAAN HARIMAU SUMATERA PADA KONSERVASI EX-SITU DI TAMAN MARGASATWA RAGUNAN DAN TAMAN MARGASATWA BANDUNG Yolanda, Yolanda; Rusdi, Rusdi; Supiyani, Atin
Bioma Vol. 13 No. 2 (2017): Bioma
Publisher : LPPM Universitas Negeri Jakarta

A zoo is one of an ex-situ conservation activity whice aimed to conserve plants and wildlife animal an endangered position, for example; the Panthera tigris sumatrae population are decrease. The zoo were succeed to captivate an animal only if they can adopt their normal life into that place. The aim of this research was to study the animal welfare from Sumatran tiger in two different places; Bandung zoo and Ragunan zoo. This research has been conducted in Desember â€“ February 2017. The research method that are used in this study were interviewed and filling draft animal welfare from Indonesian Ministry of Forest No.P.9/VI-SET/2011. The Result of this research were 77% point for Ragunan zoo and 60% point for Bandung zoo which different mean both of that place. Filling draft animal welfare consist beside five freedom of animal welfare; 1). Freedom from hunger and thirst, 2). Freedom from discomfort, 3). Freedom from injury, paint, and disease, 4). Freedom to express natural behavior, 5). Freedom from fear and distress. The conclusion of this research were Ragunan zoo was better than Bandung zoo to applied animal welfare rules.

TOTAL KAROTENOID IKAN SUMATRA ALBINO (Puntius tetrazona) YANG DIBERI PAKAN TAMBAHAN TEPUNG KEPALA UDANG Tania, Novita; Sukarman, Sukarman; Permana, Asep; Supiyani, Atin
Bioma Vol. 14 No. 1 (2018): Bioma
Publisher : LPPM Universitas Negeri Jakarta

ABSTRACTAlbino tiger barb (Puntius tetrazona) is a freshwater ornamental fish that has a attractive colors. Carotenoid sources should be added in the diet because fish can not synthesis carotenoids in their bodies. One source of natural carotenoids (astaxantin and cantaxantin) is shrimp head meal, whose use has not been studied in albino tiger barb (Puntius tetrazona). The research was conducted from December 2016 until April 2017, at the Balai Riset Budidaya Ikan Hias (BRBIH) Depok. The method used in this research is an experimental method, with Completely Randomized Design (RAL). The treatment in this research is TKU supplemental feeding (0%, 10%, 20%, 30%, 40%). The data taken are total carotenoid, were analyzed using One Way ANAVA at 95% confidence level and continued with Duncan test at Î± <0,05 with SPSS 17.0 program. The result of the research is the addition of the best TKU by 30% to improve the color quality, total carotenoids, and growth of albino tiger barb (Puntius tetrazona). The highest increase in total carotenoids at the fins of 13.54 Â± 0.24 ppm and on the skin was 7.54 Â± 0.11 ppm. Keyword: Shrimp Head Meal, Carotenoid, Feed, Color Quality, Puntius tetrazona.

PRE TREATMENT MINYAK JELANTAH DENGAN KARBON AKTIF AMPAS TEBU MENURUNKAN RESIKO HEPATOTOKSISITAS TIKUS (SPRAQUE DAWLEY) Rahayu, Sri; Supiyani, Atin; Darmansyah, Rizky; Amalia, Rizka
Bioma Vol. 18 No. 2 (2022): Bioma
Publisher : LPPM Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/Bioma18(2).5

Cooking oil is a human need that is increasing over time resulting in wasted cooking oil. One of the efforts to reduce negative effect of used cooking oil is the use of activated carbon in the refinery process. The aim of this study was to determine the hepatotoxicity of used cooking oil treated with activated carbon of bagasse on rat livers. The method used true experimental laboratory with Post Test Only with Control Group Design. White rats were divided into 4 treatment groups. Positive control, given 0.2 ml of used cooking oil. Negative control, given 0.2 ml of packaged cooking oil. Group 3 was given 0.2 ml of used cooking oil mixed with 5% activated carbon. Group 4 was given 0.2 ml of used cooking oil mixed with 15% activated carbon of bagasse. The liver enzyme of SGPT-SGOT was measured by spectrophotometry and analysed by ANOVA. The concentration of bagasse-activated carbon in used cooking oil affected the levels of SGOT-SGPT and rats weight. The lowest levels of SGOT were obtained in used cooking oil treated with 5% activated carbon bagasse (18.85 ± 0.544 U/L). Application of bagasse-activated carbon in used cooking oil can reduce the risk of hepatotoxicity in mice.

Co-Authors A.S. Satyaningtijas Agussetiandari, Indah Andayani, Prawita Lintang Asep Permana, Asep Dalia Sukmawati Darmansyah, Rizky Diat Nurhidayat El Enshasy, Hesham Ali Guspri Devi Artanti Isdoni Bustaman Janovie, Arria koekoeh santoso Malintan, Rizka Mawali, Maharja Nabila, Dasilva Azka NASTITI KUSUMORINI Nurul Masyita, Nurul Octavia, Desy Oktaviani, Rahayu Prasetio, Yogi R. Haryo Bimo Setiarto, R. Haryo Bimo Ratna Komala, Ratna Rizka Amalia Rusdi Rusdi SRI RAHAYU Sukarman Sukarman Supriyatin Supriyatin Tania, Novita Tri Handayani Wijaya, Yanuar Restu Yolanda Yolanda, Yolanda Zahra, Lamya Nauratu

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Journal : Bioma : Jurnal Biologi Indonesia