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Ulyarti
Department Of Agriculture Product Technology, Faculty Of Agriculture, University Of Jambi Centre Of Excellent In Bio-Geo Material, University Of Jambi
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Journal : Indonesian Food Science and TechnologyJournal

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Measuring Protein Synthesis Rate In Living Object Using Flooding Dose And Constant Infusion Methods Ulyarti, Ulyarti
Indonesian Food Science and Technology Journal Vol. 1 No. 1 (2017): Volume 1. Number 1, December 2017 |IFSTJ|
Publisher : Department of Technology of Agricultural product (THP) Jambi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (522.406 KB) | DOI: 10.22437/ifstj.v1i1.4268

Abstract

Constant infusion is a method used for measuring protein synthesis rate in living object which uses low concentration of amino acid tracers. Flooding dose method is another technique used to measure the rate of protein synthesis which uses labelled amino acid together with large amount of unlabelled amino acid. The latter method was firstly developed to solve the problem in determination of precursor pool arise from constant infusion method. The objective of this writing is to compare the results from several researches on measuring protein synthesis rate using flooding dose and constant infusion methods and to obtain the criteria or prerequisite on the type of sample and tracer for each method. The measurements of protein synthesis rate using both methods are presented and results from both methods are compared. Both methods are eligible for measuring protein synthesis rate as long as the true precursor can be measured. The uncertainties in determining the precursor in constant infusion method and the effect of flooding on the stimulation of protein are the limitation on using the two methods. Bolus injection which imitates the flooding dose method but using smaller dose of tracer can be a good alternative in measuring protein synthesis rate.
Co-Authors Abu Bakar Abu Bakar Achmad, E Addion Nizori Asrial D. Wulansari Dedi Gusriadi Edy Panda Gusriadi, Dedi Irma Rahmayani Kanto, Rizky Lavlinesia Lavlinesia Lavlinesia Lavlinesia, L Lavlinesia, lavlinesia Leny Marlinda Marlinda, Leny Monica E. Mursalin . Mursalin Mursalin Mursalin, M Nazarudin Pamungkas, Putra Panda, Edy Pratami, Tesy Purba, Daniel Tua Putri, Mega Bunda Rafael, Fadhil Luthfi Rahayu Suseno Rahayu Suseno Rahmawati, Medina Alia Rahmayani, Irma Ramadani, Suci Putri Rizky Kanto S. E. Sitinjak S.L Rahmi Silvi Leila Rahmi Slamet Slamet Sulistiana, Ika Putri Surhaini Surhaini, Surhaini Suseno, Rahayu Yani, Zunarta Yusfi, Liza Aulia Zunarta Yani